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Citations for Millipore Sigma :
8901 - 8950 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... HMCES-3flag protein was detected by immunoblotting with anti-flag antibody (1:10,000 dilution in PBST; Sigma, F1804-200UG) and rat anti-mouse secondary antibody peroxidase conjugate (1:20,000 dilution in PBST ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Biochemistry 2023Quote: ... Y730F)41 was cloned into pET28a vector incorporating a His6-tag and a SUMO tag at the amino terminus of the expressed protein and expressed in E.coli BL21 gold strain (Novagen). Cells were grown at 37°C in TB medium containing 0.1 mg/ml kanamycin and 0.050 mg/ml tetracyline to an OD600 of ∼3.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the eluted proteins were subjected to Western Blot analysis using a GST antibody (1:1000, Millipore 05-782).
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Plant Biology 2023Quote: ... Frozen tissue was homogenized with 2 volumes of protein extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Biophysics 2023Quote: ... As per the manufacturer’s recommendation a protein standard curve was first measured using Bovine Serum Albumin (P 0384, Sigma) standards ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μg of soluble proteins in TEAB buffer were added onto 30 kDa cutoff filter (Sigma-Aldrich, cat#MRCF0R030) and centrifuged at 11,000 g for 15 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using centrifugal filter units (Amicon, 10KDa cut-off; Millipore, Ultra-4 UFC801024 and/or Ultra-0.5 UFC501024) ...
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bioRxiv - Immunology 2023Quote: ... Proteins were subsequently transferred onto nitrocellulose membranes and immunoblot analysis was performed with following antibodies: HA (Sigma-Aldrich, H6908), FLAG (M2 ...
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bioRxiv - Microbiology 2023Quote: ... The gel was first washed with water and then incubated with c.a 10 mL of InstantBlueTM Ultrafast Protein Stain (Sigma) for at least 1 hour.
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bioRxiv - Cancer Biology 2023Quote: ... was purified as described (Noland et al., 2016) with the exception that the proteins were treated with 2.5% hydroxylamine (Sigma) at pH 7.0 for one hour at room temperature prior to size exclusion chromatography ...
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bioRxiv - Cancer Biology 2023Quote: ... equal amounts of protein per sample were subjected to SDS–PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore). For immunoprecipitation of FLAG-tagged proteins ...
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bioRxiv - Synthetic Biology 2023Quote: ... The quality of protein transfer was visualized by staining with Ponceau S (0.1% dye in 5% acetic acid, Sigma) and then blocked with 5% nonfat instant milk (Carnation ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Biochemistry 2023Quote: ... proteins were imaged using Immobilon Classico Western HRP substrate or Immobilon Ecl Ultra Western HRP substrate (Millipore, MA, USA) on the iBright FL1500 Imaging System (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... 4µg of the appropriate antibody and 20µl of Magnetic protein A/G beads (Magnachip, EMD-Millipore, Billerica, MA, USA) were used for the IP step ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was eluted with 4 CVs of imidazole-free wash buffer supplemented with 150 µg/mL 3xFLAG peptide (Sigma). This was analysed by SDS-PAGE before being concentrated ...
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bioRxiv - Microbiology 2023Quote: For top-down EF-P measurements the purified proteins were desalted on the ZipTip with C4 resin (Millipore, ZTC04S096) and eluted with 50 % (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein samples were run on EZ Protein any KD PAGE (LIFE-iLAB) and then transferred to 0.22um PVDF membranes (Millipore). Membranes were blocked using 5% non-fat milk in PBS-Tween20 0.1% for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed protein solution was then concentrated using Amicon Ultra-4 Centrifugal Filter Units 30 K (Millipore, MA #UFC803024) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The following commercially available antibodies were used to detect targeted proteins: anti-RBM17 (1:1500 dilution; HPA037478, Sigma-Aldrich), anti-SF3B1 (1:3000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess reagents were removed by MeOH/CHCl3 protein precipitation or concentration-dilution using a 0.5 mL Centrifugal Filter (3K, Millipore). Pellets were washed by 500 μL MeOH/H2O (9:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 30–50 μg of protein was fractionated by 10% SDS–PAGE and blotted onto an Immobilon PVDF membrane (Millipore). Non-specific binding sites were blocked by incubation with 5% BSA in Tris-buffered saline (TBS ...
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bioRxiv - Biophysics 2023Quote: ... Lysis was done either by resuspending the cell pellet in 0.8mL of the detergent-based BugBuster Protein Extraction Reagent (Millipore) and agitating for 20min on a rotator ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein complex was eluted with 500 μL elution buffer (0.4 mg/ml 3X Flag peptide (Millipore Sigma F4799) in wash buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were buffer exchanged into 1x PBS (pH8.0) using Amicon Ultra-15 Centrifugal Filter Unit (10 kDa) (Millipore) and flash-frozen.
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bioRxiv - Cell Biology 2024Quote: ... before clarification by centrifugation and enrichment for FLAG-tagged proteins using anti-FLAG® M2 Affinity Gel (A2220, Sigma), with elution using 3xFLAG peptide (F4799 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were concentrated by filtration of the eluate in 15-mL Amicon® Ultra 50k filters (Millipore, Burlington, MA). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: Equal amounts of proteins from total cell lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The blots were blocked with 5% w/v BSA and 0.05% NaN3 in TBST and incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Biophysics 2024Quote: ... Each protein was dialyzed and concentrated against 100LmM ammonium acetate (pH 7.4) using an Amicon 0.5-mL microconcentrator (EMD Millipore) with a 10-kDa MW cutoff and stored at –80L°C until used ...
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bioRxiv - Biophysics 2024Quote: ... The target protein was eluted by application of 5 CVs of buffer E containing 2.5mM d -desthiobiotin (Sigma-Aldrich). Elutions were pooled ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection of specific protein bands was accomplished using a rabbit monoclonal anti-GLRX3 antibody (1:1,000, HPA028941, Sigma-Aldrich), ferritin (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... and FLAG-tagged proteins were detected by alkaline-phosphatase-conjugated anti-FLAG IgG (A9469, Sigma Aldrich, St. Louis, MO) and 1-Step NBT/BCIP substrate solution (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg mL-1 catalase from bovine liver (lyophilized powder, 2000-5000 units/mg protein, Sigma-Aldrich, Missouri, US) and 50 mM SPB (pH 7.2 ...
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins were transferred from the gel to Immobilon®-P PVDF membranes (Millipore Sigma, Cat# IPVH00010, USA) in 10x Tris/Glycine buffer (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... 50 µM mutant protein solution was mixed with excess amount of Atto dye in respective binding buffer (Sigma Aldrich) and incubated overnight at 4°C in a dark place ...
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bioRxiv - Biophysics 2024Quote: ... Refolded proteins were further purified by size-exclusion chromatography using a Hiload 16/600 Superdex 75 column (Sigma-Aldrich) in buffer A.
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bioRxiv - Plant Biology 2024Quote: ... Tissue was ground in ice-cold protein extraction buffer (150 mM NaCl, 50 mM Tris-HCl [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was filtered through polyvinylidene fluoride ultra-free membrane filter with a 0.22 μm pore size (Millipore). The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were incubated at 50 µM in the presence of 10x excess of Bromo-1,1,1-trifluoroacetate (BTFA, Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated to 2.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was eluted with GFB containing 2.5 mM desthiobiotin and concentrated in a 10 kDa MWCO concentrator (Millipore Sigma). Concentrated protein was loaded onto the Superdex 75 Increase 10/300 GL (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... CHIKV nsP1 protein was concentrated to the required concentration with Amicon centrifugal filters (10,000 MWCO Millipore, Burlington, MA, USA), flash-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins from the purified PB or pre-sorting fractions were dissolved in a 8 M urea solution (Sigma-Aldrich) before trypsin digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... The fractions that contained the SsCBE2-C2 protein were collected and concentrated through an Amicon Ultra centrifugal filter (Millipore). Following that ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were detected after incubating with peroxidase conjugated secondary antibodies for 1 hour and 5min with ImmobilonR Western (Millipore) after 5x washes ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing the expressed protein were combined and concentrated to 2.5 mL with a 30-kDa cut-off centrifugal filter (Millipore), run through the desalting column again ...