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Citations for Millipore Sigma :
8851 - 8900 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells (100% confluent) were harvested and lysates prepared using CytoBuster protein extraction reagent (Novagen/EMD, Gibbstown, NJ) supplemented with complete mini EDTA-free protease inhibitor (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Different amount of protein samples were electrophoresed on 10% SDS-PAGE and then transferred to PVDF membranes (Millipore, Merck) by electroblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were resuspended in 150µL wash buffer 3 and diluted with two volumes of 10 mM Tris pH 8.5 prior to on-bead digestion of proteins via addition of 500 ng modified sequencing grade Trypsin (Sigma) with incubations of 1 hour at 4°C and then overnight at RT with continuous mixing at 1200 rpm ...
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bioRxiv - Neuroscience 2023Quote: The eluent containing the target proteins underwent filtration using a Centricon filter with a 100 kD cutoff (EMD Millipore). The Centricon filter was then centrifuged at 1,200g for 10 minutes until the sample volume was reduced to less than 1 mL ...
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bioRxiv - Plant Biology 2023Quote: Frozen leaf tissue was ground in two volumes of protein extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2023Quote: ... and 100 µM Na3VO4) before the protein was eluted with FLAG wash buffer containing 0.5 mg/mL 3xFLAG peptide (Sigma). LIMK1 concentration was determined by running alongside BSA standards on SDS-PAGE with Coomassie staining.
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Cancer Biology 2023Quote: ... 210 ug of protein was digested using Lys C (Wako) at an enzyme:protein ratio of 1:100 and trypsin (Sigma) at 1:50 ratio overnight at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... a booster injection of 100 µg of Tin protein in 200 µl of Freund’s Incomplete Adjuvant (Sigma-Aldrich 344291) was injected at four sites ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein samples were concentrated to 1-2.5 mg/mL using a concentrator (cut-off: 100 kDa, Amicon Ultra, Millipore). Representative SEC profiles and Coomassie blue stained SDS-PAGE gels for the Closed ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-BRI2 proteins were immunoprecipitated with anti-FLAG mouse monoclonal antibody M2 cross-linked to agarose beads (Sigma A2220); immunoprecipitated proteins were eluted with 3X FLAG peptide (Sigma F4799) ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins from cleared lysates was performed with Anti-Flag M2 Affinity Agarose Gel (Sigma-Aldrich) at 4°C for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: MCF10A JUN knockout cells stably expressing BirA*-c-JUN fusion proteins were treated with 50 μM biotin (Sigma-Aldrich) for 18 h ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to a PROTRAN 0.2 µm nitrocellulose membrane (Cytiva) and stained with Ponceau S (Sigma-Aldrich). Membranes were incubated in 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... An equal amount of protein was analyzed using SDS-PAGE and transferred to polyvinylidene fluoride PVDF membranes (0.22 μm pore size, Millipore). The membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Microbiology 2023Quote: ... The dialyzed protein was concentrated to the required concentration with amicon centrifugal filters (10,000 MWCO Millipore, Burlington, MA, USA) and frozen in liquid nitrogen and stored at −80 °C till its use.
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bioRxiv - Immunology 2023Quote: Male mice were sensitized with 4 mg of PN butter (∼1 mg of protein; Capitán Mani) and 5 μg of cholera toxin (C8052, Sigma) in 0.5 mL of PBS administered intragastrically ...
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bioRxiv - Molecular Biology 2023Quote: Uniformly 15N,13C-labelled recombinant Aβ40 was produced as follows: Aβ40 was expressed as a SUMO fusion protein using a modified pET32a vector (Novagen). Protein expression was performed in E ...
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bioRxiv - Microbiology 2023Quote: ... The DNA in 50 µL of water was immunoprecipitated with 20 µg/mL anti-protein A antibodies (Sigma Aldrich) and purified by phenol/chloroform extraction ...
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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Astroglia proliferate upon biogenesis of tunneling nanotubes and clearance of α-synuclein toxicitiesbioRxiv - Cell Biology 2023Quote: ... Then the protein was labelled adding two-fold molar excess of TetramethylRhodamine-5-maleimide (TMR-maleimide; Sigma Aldrich #94506) in 20 mM HEPES buffer (pH7.4) ...
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bioRxiv - Microbiology 2023Quote: ... β-mercaptoethanol (99%) for 6 h at 4 ℃. The proteins were concentrated by Amicon Ultra (M.W. 3,000 Da) (Sigma-Aldrich) and stored at −80 ℃.
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bioRxiv - Molecular Biology 2023Quote: ... and the eluted proteins were subjected to Western Blot analysis using a GST antibody (1:1000, Millipore 05-782).
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bioRxiv - Biochemistry 2023Quote: ... Y730F)41 was cloned into pET28a vector incorporating a His6-tag and a SUMO tag at the amino terminus of the expressed protein and expressed in E.coli BL21 gold strain (Novagen). Cells were grown at 37°C in TB medium containing 0.1 mg/ml kanamycin and 0.050 mg/ml tetracyline to an OD600 of ∼3.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Infected CAD5 cells were seeded in ELISPOT plates (multiscreen 96-well filter plates with high protein-binding Immobilon-P membrane, 0.45 µm, Millipore) activated with 70% ethanol ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression and native purification was performed by using His-select Nickel affinity gel resin column (Sigma Aldrich, USA) according to the manufacture’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... followed by resuspension of the dry pellets by vortexing (≈1 min) and subsequent addition of 150 μL lysis buffer (HEPES buffer containing 0.35X BugBuster Protein Extraction Reagent (Novagen) and 0.1% Lysonase Bioprocessing Reagent (Novagen)) ...
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bioRxiv - Biophysics 2023Quote: ... a solution of 50 μM αS and 1 μM N-protein in 20 mM Tris buffer (Sigma-Aldrich, UK), pH = 7.4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of total proteins (20μg) were separated by SDS-PAGE and transferred to a 0.45-mm Immobilon-P PVDF Membrane (Millipore, Merck) and the membrane was incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and Tissue Protein Extraction Reagent (Pierce, Waltham, MA, USA) supplemented with 2.5% of 2-mercaptoethanol (Sigma Aldrich, Darmstadt, Germany). Cell lysates were boiled for 8 min and stored at − 80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The total protein was then resolved on SDS-PAGE (8-12%) and transferred onto PVDF membrane (Millipore, Massachusetts, USA). After the transfer ...
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bioRxiv - Biophysics 2022Quote: ... The eluted protein was buffer exchanged with PBS (pH 7.4) and was concentrated using 10 kDa cut off Amicon (Merck Millipore) concentrator to a final concentration of 1 mg/ml and stored at −80°C until further use.
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bioRxiv - Immunology 2023Quote: ... Culturing and induction of protein expression following application of the inductor isopropyl-β-D-thiogalactopyranoside (IPTG; Sigma, Deisenhofen, Germany)) was performed as previously described (33).
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Biochemistry 2023Quote: ... MWRA flow-through protein solution was concentrated to ∼15 mL using a 30 kDa MWCO centrifugal filter (EMD Millipore), ensuring not to concentrate to where solution became yellow/cloudy and viscous ...
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bioRxiv - Biochemistry 2023Quote: 50 µg of refolded TREM2 protein in 100 mM ammonium bicarbonate buffer was digested with 2 µg trypsin (Sigma) at 37 °C for overnight ...
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bioRxiv - Neuroscience 2023Quote: Transduced CHO-K1 cells expressing hα7-nAChR and chaperone proteins were cultured on 50µg/ml poly-D-lysine (Sigma-Aldrich) and 330µg/ml Collagen type 1 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then spun at 21,000 g for 15 min at 4°C and protein concentration determined using Bradford assay (Sigma). Approximately 3-4mg of total cell extract was mixed with 20μl of M2-anti-FLAG agarose beads (bed volume ...
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bioRxiv - Biochemistry 2023Quote: ... the protein (with N-terminal His6 tag) was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 8.11 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane (Amersham Protran, GE10600001, Sigma Aldrich) by the wet transfer method ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Biophysics 2023Quote: ... The calibration was performed using a protein standard mixture: of β-amylase (Sigma-Aldrich, 56, 112, and 224 kDa), and thyroglobulin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then transferred to a nitrocellulose membrane and blocked with PBS + 0.1% Tween 20 + 5% Milk powder (Sigma) and incubated overnight with primary antibodies at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... These lysates were then additionally treated for protein precipitation with 3 volumes of ultra-pure methanol (Sigma-Aldrich, USA). Ultimately ...
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bioRxiv - Biochemistry 2022Quote: ... HMCES-3flag protein was detected by immunoblotting with anti-flag antibody (1:10,000 dilution in PBST; Sigma, F1804-200UG) and rat anti-mouse secondary antibody peroxidase conjugate (1:20,000 dilution in PBST ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Biophysics 2023Quote: ... As per the manufacturer’s recommendation a protein standard curve was first measured using Bovine Serum Albumin (P 0384, Sigma) standards ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μg of soluble proteins in TEAB buffer were added onto 30 kDa cutoff filter (Sigma-Aldrich, cat#MRCF0R030) and centrifuged at 11,000 g for 15 minutes at RT ...