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Citations for Millipore Sigma :
8801 - 8850 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and mNedd4△N52 protein fusions were expressed in Rosetta2(DE3)-pLysS cells grown in 2xYT medium (Millipore Sigma, Y2627) at 37°C until the optical density (OD ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were separated by electrophoresis through 12% polyacrylamide gels containing SDS (0.1%) and transferred to a PVDF membrane (Millipore). The membrane was blocked with 2% (w/v ...
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bioRxiv - Genomics 2022Quote: ... Proteins from whole cell lysate were separated in Laemmli buffer by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2022Quote: ... Recombinant Fab protein was eluted with 50 mM acetate buffer at pH 5.0 and concentrated using Ultra-15 Centrifugal Filters (Millipore). Protein concentration was determined using the Qubit protein assay (Invitrogen).
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bioRxiv - Plant Biology 2022Quote: ... the coding sequence for the mature STIC2 protein (aa 49-182) was cloned into BamHI/SalI linearized pETDuet1 (Novagen) and pGEX4T3 (Cytiva ...
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bioRxiv - Immunology 2022Quote: ... After concentrating the protein using a 30 kDa molecular weight cut-off Amicon Ultra-15 centrifugal filter unit (Millipore) the protein was further purified by size-exclusion chromatography using a Superose 6 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... the proteins were concentrated with Amicon Ultra-15 centrifugal units with a molecular cut-off of 30 KDa (Sigma).
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bioRxiv - Plant Biology 2022Quote: ... 40 μg total proteins were loaded into 10% acrylamide gels and transferred to Immobilon-P membrane (Millipore, Cat. IPVH00010). The antibody were diluted as follows ...
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bioRxiv - Microbiology 2022Quote: ... 500nM of (GlcNAc)6 were added to trigger ROS production in the absence or presence of 1μM of effector protein in a measuring solution containing 100μM of L012 substrate (Sigma Aldrich) and 40μM horseradish peroxidase (Sigma Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... The eluate was then dialyzed overnight in PBS and protein was concentrated by using Amicon Pro centrifugal filter (Millipore). The protein was quantified by using BCA Protein Assay Kit (Thermofisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Size standards were generated using a gel filtration marker kit of protein molecular weights that ranges from 29,000 to 700,000 (Sigma-Aldrich).
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bioRxiv - Plant Biology 2022Quote: ... immunoblotting was performed with 50 μg of protein extracts using anti-Flag-M2-HRP (Sigma-Aldrich, A8592; 1:10,000), anti-c-myc-peoxidase (Roche ...
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Local induction of IAA5 and IAA29 promotes DNA damage-triggered stem cell death in Arabidopsis rootsbioRxiv - Plant Biology 2022Quote: ... Chromatin bound to SOG1-Myc proteins extracted from whole seedlings was precipitated with anti-Myc antibody (clone 4A6, Millipore). To quantify the precipitated chromatin ...
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bioRxiv - Plant Biology 2022Quote: ... Then total proteins were resolved in 8% SDS-PAGE and transferred to immobilon-P membrane (MILLIPORE cat. No. IPVH00010). GFP antibody (trans #HT801) ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 M NaCl) before eluting bound proteins with buffer A containing 0.5 M Methyl-α-D mannose (Sigma). Fractions containing NA activity were pooled ...
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bioRxiv - Microbiology 2023Quote: ... and proteins were concentrated by 30 kDa cut-off Amicon Ultra-15 Centrifugal Filter Units (Millipore, Cat. No. UFC903096).
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bioRxiv - Immunology 2022Quote: ... Protein extracts were loaded onto 8/15% polyacrylamide gels and then transferred onto a PVDF membrane (Millipore, Burlington, MA). Membrane was then blocked by incubating in 5% skim milk/TBS for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Protein expression from hybrid lac promoters was induced by addition of isopropyl-b-D-thiogalactopyranoside (IPTG 0.25 mM; Sigma) where indicated.
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bioRxiv - Physiology 2022Quote: ... 80 μg of protein were incubated in 10 mM L-cysteine and 1 mM pyridoxal 5′-phosphate hydrate (Sigma); or ...
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bioRxiv - Molecular Biology 2022Quote: ... The filters were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (07-181, Millipore), p-AMPK (#2535 ...
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bioRxiv - Plant Biology 2022Quote: ... Either total membrane or immunoprecipitated proteins were separated on a SDS-PAGE gel and transferred to PDVF membrane (Millipore) for immunoblot analysis using monoclonal anti-GFP (33-2600 ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysates were incubated with 1.5 µg polyclonal anti-V5 or IgG for 1 h and captured with protein A agarose resin (P2545, Sigma) for 30 min ...
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bioRxiv - Systems Biology 2022Quote: ... The lysate was centrifuged at 4,000 xg for 5 min at 4°C and the protein pelletwas resuspended in Digestion Buffer(10% 2,2,2-Trifluoroethanol (Sigma; #96924)) in 100 mM HEPEs pH 8.5) ...
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bioRxiv - Molecular Biology 2022Quote: ... Nanobody fusion proteins were detected using a primary anti-HA (mouse; 1:3,000, Sigma-Aldrich, St. Louis, MO, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2022Quote: Cleaved protein was concentrated to ~1 mL using an Amicon Ultra-15 centrifugal filter 30 kDa MWCO (EMD Millipore), then loaded onto a HiLoad Superdex S200 pg gel filtration column (GE Healthcare Life Science) ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein (20 μg) was electrophoresed on 15% SDS-polyacrylamide gel and transferred to PVDF (Millipore, Burlington, MA, USA) membranes ...
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bioRxiv - Cell Biology 2022Quote: ... followed by treatment with protein synthesis inhibitor cycloheximide (CHX) (Cat# C-7698, Sigma, stock 100 mg/ml in DMSO) at 0.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were collected at 42-48 h after transfection and filtered through a 0.45 um low-protein binding filter (Millipore). Patient-derived TCL were infected with lentiviral supernatants containing polybrene (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... resuspended in 1 mL total protein extraction buffer (10 mM Phosphate Buffered Saline (PBS) at pH 7.4 (Sigma P5368), 0.5% v/v NP-40 (Nonidet™ P 40 Substitute from Sigma 74385) ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated using sodium dodecyl-sulfate polyacrylamide gel electrophoresis and electrophoretically blotted onto microporous polyvinylidene fluoride membranes (Millipore) under a constant current ...
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bioRxiv - Bioengineering 2024Quote: ... and secreted proteins in media were harvested after 120 hours and cleared using a 0.45-µm PVDF vacuum filter (Sigma Millipore). Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen ...
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bioRxiv - Immunology 2023Quote: Protein expression was stably knocked down in Jurkat T cells by RNA interference using Mission shRNA plasmids (Sigma-Aldrich). Lentiviral particles were generated by transfecting HEK293T cells with pMD2G ...
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bioRxiv - Molecular Biology 2023Quote: ... Pure protein-containing fractions were pooled and concentrated using centrifugal spin concentrators with an MWCO of 30 kDa (Millipore). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... and expression of the GFP-tagged protein was verified by western blot using an anti-GFP antibody (Sigma-Aldrich). Colonies with the highest expression for a given construct were used to inoculate 300 mL of YEPD media containing 200 µg/mL Zeocin and grown overnight with shaking (220 rpm ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluates were then desalted and concentrated by ultrafiltration with Amicon Ultra 0.5 10K (Merck Millipore, Burlington, Massachusetts, USA) columns ...
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bioRxiv - Molecular Biology 2023Quote: ... The various 3xF tagged NRPD1 and CLSY proteins were detected using a monoclonal anti-FLAG-HRP antibody (Sigma #A8592). RDR2-eGFP was detected using an anti-eGFP polyclonal antibody68 ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the equivalent of 500-800 µg of protein was incubated with 1 µg of anti-GFP antibody (Sigma-Aldrich) and then incubated with Protein A/G magnetic beads (Thermofisher ...
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bioRxiv - Microbiology 2024Quote: ... The purified protein was concentrated to ∼1 mg/mL by centrifugal filtration (AmiconTM 30K, Millipore Sigma, Burlington, MA, USA), flash frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2024Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich, St. Louis, USA). After purification ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing the purified protein were pooled together and concentrated using 30-kDa cut-off centrifugal devices (Millipore). Further ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein was concentrated to 0.6 mL using a 10 kD cut-off Amicon Ultra centrifugal filter (Millipore). The sample was then loaded into a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: Total protein from UM51- and B4-derived neuronal cultures was isolated using RIPA buffer (Sigma-Aldrich Chemicals, Taufkirchen, Germany), supplemented with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... conditioned media was isolated and filtered through a 0.4µm low protein binding PVDF Miltex syringe-driven filter (Millipore #SLHV033RS) to remove cellular debris ...
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bioRxiv - Cancer Biology 2024Quote: ... Conditioned media was isolated and filtered through a 0.4µm low protein binding PVDF Miltex syringe-driven filter (Millipore #SLHV033RS) to remove cellular debris ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated on 6-10% SDS-PAGE gels and transferred to methanol pre-activated PVDF membranes (Millipore, #IPVH00010). For MYCBP2 blots ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cells were lysed on ice for 30 min in the manufacturer’s lysis buffer supplemented with 0.5% Triton-X100 and complete EDTA-free protein inhibitors (Sigma-Aldrich), with gentle 5s vortexing every 5 min ...