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Citations for Millipore Sigma :
8701 - 8750 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the protein extracts (15 μg/sample) were blotted on a primed 0.45 μm polyvinylidene fluoride (PVDF) membrane (Merck-Millipore). Protein spots were stained using Direct Blue (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins samples (20 μg/lane) were separated by SDS–PAGE and transferred onto PVDF membranes (0.45μm, Millipore, USA) using a transfer unit (Bio–RAD ...
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bioRxiv - Genomics 2022Quote: ... Proteins from whole cell lysate were separated in Laemmli buffer by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2022Quote: ... Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam ...
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bioRxiv - Immunology 2022Quote: ... DENV infection was then measured by intracellular detection of the E protein using the murine monoclonal 4G2 antibody (Millipore) followed by rabbit anti-mouse IgG-coupled to AF647 (Molecular probes) ...
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bioRxiv - Cancer Biology 2022Quote: ... equal protein amounts were collected across the samples and subjected to 25 μM DBCO-PEG4-biotin (Sigma-Aldrich, #760749) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... The glass surfaces were coated with 1 mg/mL of BSA protein (Albumin, biotin-labeled bovine, A8549 Sigma-Aldrich) by incubating for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: The coverslips now containing fixed spheroplasts were incubated in protein crosslinking prevention solution (2% acrylamide (AA, Sigma, cat.no. A4058) / 1.4% formaldehyde (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved on 12% SDS–PAGE and proteins were transferred to methanol-activated polyvinylidene fluoride (PVDF) membrane (Millipore) using anode buffer (25 mM Tris HCl pH 10.4 ...
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bioRxiv - Biochemistry 2019Quote: The proteins in an SDS-polyacrylamide gel were transferred onto an Immobilon®-P PVDF transfer membrane (Millipore Sigma) by electroblotting at 250mA for two hours in ice cold transfer buffer (25mM Tris ...
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bioRxiv - Plant Biology 2019Quote: ... and bound protein was eluted with 60 μL of 0.5 mg/mL 3×FLAG peptide (Sigma-Aldrich, MO, USA) for 1 h at 4°C ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... The lysates were then precleared for 1h at 4°C using 10µl protein A agarose 50% slurry/plate (Millipore), and collected by centrifugation at 300g for 3min at 4°C (100µl of sample/plate was kept as input chromatin) ...
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bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was allowed to proceed by rotation at 4°C for 2hrs and then immunoprecipitated with 60μl Protein G Sepharose (Sigma). The sediments were washed twice with HEPES/KCl buffer + 0.25% Triton-X100 ...
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bioRxiv - Cancer Biology 2019Quote: ... The collected lysate proteins were then subjected to immunoblotting with LC3 (L7543) and β-actin (A5441) antibodies (Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Expression of MxA proteins was detected by Western blot analysis using mouse ANTI-FLAG® monoclonal antibody F4042 (Sigma) and rabbit anti-beta-actin polyclonal antibody ab8227 (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Fractions containing monomeric protein were pooled and concentrated to around 50 μM using Amicon Ultra-0.5 ml Centrifugal Filters (Millipore), and then aliquoted and snap-frozen until further use.
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bioRxiv - Biochemistry 2019Quote: ... and proteins were resuspended in 100 μL of 100 mM ammonium acetate and 1 μg trypsin (Proteomics grade, Sigma). Proteins were digested by incubation at 37 °C for 16 h.
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bioRxiv - Cell Biology 2019Quote: ... lysates were immunoprecipitated with ACC antibody (CST) or ACC2 antibody (CST) and EZview Red Protein G Affinity Gel (Sigma).
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bioRxiv - Cancer Biology 2019Quote: ... activity in each cell line was assayed as described30 using 6.7 µg of WCE proteins in the presence or absence of 300 µM of CES2 inhibitor loperamide (LOP, Sigma) in 20 µL of 100 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2019Quote: ... proteins were transferred to a PVDF membrane and immunoblotted with an anti-phosphotyrosine antibody (1:1000 dilution, Upstate/Millipore) for chemiluminescence detection.
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bioRxiv - Plant Biology 2019Quote: ... a C-terminal polypeptide encompassing the last 284 amino acids of the PKL protein has been expressed in fusion with a hexahistidine tag in the E.coli strain BL21DE3 Rosetta (Novagen). The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose ...
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bioRxiv - Neuroscience 2019Quote: ... The proteins were then precipitated using acetonitrile (400 µL) containing 0.1% v/v formic acid (LC-grade, Sigma-Aldrich). This was done in metal and glass syringes to ensure no contamination from plastic ...
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bioRxiv - Pathology 2020Quote: ... cell lysates containing equal protein amounts were separated by SDS-PAGE and transferred to PVDF membranes (Cat.#ISEQ00010, Millipore). Membranes were sequentially probed with primary antibodies and appropriate peroxidase-conjugated secondary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4.2 μg PARG (in-house) per 10 mg protein and 10 μl of Benzonase (Merck Millipore, Darmstadt, Germany) per 30 ml were then added to each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... Binding and crosslinking of the GST-Af1521 fusion proteins to Glutathione Sepharose 4B beads (Sigma Aldrich, Saint Louis, Missouri), peptide enrichment (“standard workflow” ...
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bioRxiv - Biophysics 2020Quote: ... 200 μL of 50 μM protein aliquots were reacted with 50 molar equivalents of methyl 2,5-dibromopentanoate (Sigma Aldrich) for 12 hours at 37 °C and shaking at 500 rpm ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 12 mg RBD protein in 3 mL PBS was mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the second boosting ...
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bioRxiv - Molecular Biology 2020Quote: ... One milligram of total cellular protein was then incubated with 30 μl bed volume of anti-V5 beads (Sigma), which were pre-equilibrated in buffer A ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein samples were separated by 10% SDS-PAGE gel and then electrically transferred to 0.45μm PVDF membranes (Millipore, IPVH00010). Membranes were then blocked with 5% non-fat milk in TBST for one hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cleaved TREK1 or TRAAK protein was concentrated in 50 kDa molecular weight cutoff (MWCO) Amicon Ultra Centrifugal Filters (Millipore) and applied to a Superdex200 10/300 gel filtration column (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of the target proteins was verified by western blot using ANTI-FLAG M2 Monoclonal Antibody (Sigma-Aldrich). ChIP assay was performed with ANTI-FLAG M2 Magnetic Beads (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... coli genes for proteins bS6 and bL9 were PCR-amplified and cloned individually into the pET28(a) plasmid (Novagen). Recombinant single-cysteine proteins were then expressed ...
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bioRxiv - Immunology 2020Quote: ... one 10-cm dish of packaging cell line at roughly 70% density was transfected with the target construct and two supplementing vectors providing the VSV gag/pol and VSV-G-env proteins (6 μg of DNA each) using polyethylenimine (22.5 μg/ml, Sigma) and Polybrene (4 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing the proteins of interest were pooled and concentrated using Amicon Ultra-15 30K spin concentrators (Merck Millipore) before further purification using a HiLoad 16/600 Superdex 200 prep grade size exclusion column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Cells were centrifuged and resuspended (50 µl per 1 mL of sample) with SDS sample loading buffer (msGFP2 fusions) or BugBuster Protein Extraction Reagent with 0.1% lysonase (Millipore) followed by SDS loading buffer (3xFLAG fusions) ...
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bioRxiv - Pathology 2021Quote: ... Japan) were immunized (four times at 2-week intervals) with the purified protein conjugated to TiterMax Gold (Sigma-Aldrich). Rabbits were sacrificed under excess anesthesia with pentobarbital sodium (64.8 mg/kg) ...
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bioRxiv - Biophysics 2021Quote: ... A set of sequence ladders for the MS2 coat protein was obtained by addition of the four ddNTPs (Sigma) in a final concentration of 1 mM each to the M-MulV reaction mix containing dNTPs at 0.25 mM each ...
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bioRxiv - Pathology 2021Quote: ... 10 mM Na4P2O7) in the presence of protease inhibitors (completeTM, Mini, EDTA-free Protein inhibitor cocktail tablets, Roche, Sigma). Gut tissue and GDE were homogenized in the lysis buffer and sonicated three time for 30 seconds each at 10 μm amplitude ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were detected with antibodies after western blotting: ABCE1-TAP with peroxidase anti-peroxidase (PAP) complex (Sigma-Aldrich) at 1:2,000 ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions contained labelled protein were pooled and concentrated with Amicon Ultra-15 50 kDa cutoff centrifugal filter units (Millipore), frozen with liquid nitrogen and stored at −80°C ...
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bioRxiv - Physiology 2020Quote: ... the protein content of each sample was quantified using the Bradford assay (B6916, Sigma Aldrich, Saint Louis, MI, USA) with protein standards made from bovine serum albumin (ALB001.25 ...
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bioRxiv - Neuroscience 2020Quote: ... 5μg of total cell lysate proteins were resolved on 4-12% SDS-PAGE and transferred onto 0.45 μm polyvinyl difluoride membrane (Millipore) as described above under western blotting of EVs.
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bioRxiv - Immunology 2021Quote: ... the protein aliquots were transferred to the corresponding buffer with the use of Amicon Ultra-0.5 Centrifugal Filter Units (Millipore). The NaOCl sensitivities of intact cpYFP ...
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bioRxiv - Biochemistry 2020Quote: ... MBP-DIX proteins were eluted with three column volumes of lysis buffer containing 10 mM D-(+)-maltose monohydrate (Sigma) applied to loose resin under gentle rotation for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifuged at 20000g for 15mins and filtered through a low-protein binding 0.45µm PVDF membrane syringe filter (Millipore, #SLHVX13NL), the extracts were incubated with 30µl mouse anti-HA agarose beads (clone HA-7 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The Strep-tagged MGE proteins were eluted with buffer A supplemented with 2.5 mM desthiobiotin (Novagen and Sigma-Aldrich). The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The Strep-tagged MGE proteins were eluted with buffer A supplemented with 2.5 mM desthiobiotin (Novagen and Sigma-Aldrich). The protein composition of the eluent was verified by western blot using Strep-tag II monoclonal antibodies (Novagen) ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing the protein were pooled and concentrated by centrifugal concentrators with 3 kDa molecular weight cutoff (EMD Millipore). The sample was polished by gel filtration (120 ml Superdex200 ...