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Citations for Millipore Sigma :
8601 - 8650 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were PCR-amplified with 12-13 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB ...
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bioRxiv - Cell Biology 2024Quote: ... 3 day old transgenic animals were anaesthetized on 2% agarose pads containing 2 mM levamisole (Sigma, L9756). DAF-28::GFP expression pattern was examined in at least 30 animals on day 3 of adulthood ...
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bioRxiv - Biochemistry 2024Quote: ... Arp2/3 iso-complexes were incubated with 100 µM CK-666 or CK-869 (Sigma, Bio-Techne), or equivalent amount of DMSO at room temperature for 1 hour before being used in in vitro assays.
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bioRxiv - Cancer Biology 2024Quote: OVCAR-3 cells were treated with s-p-Bromobenzylglutathione cyclopentyl diester (Glo-1 inhibitor) (SML1306, Sigma-Aldrich) for 72 h.
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bioRxiv - Cancer Biology 2024Quote: Cell viability was assessed using colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay following by the manufacturer’s instructions (Sigma). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: Glass coverslips of 20×20 µm are coated with silane (2% (3-Trimethoxysil) propyl-methacrylate (Sigma #M6514)) ...
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bioRxiv - Immunology 2024Quote: Explants were made by incubating fresh primary tumor tissue overnight in collagenase A (3 mg/mL; Sigma) in MammoCult media (StemCell technologies ...
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bioRxiv - Physiology 2024Quote: ... Sections were deparrafinized and rehydrated in sequential 3-minute soaks in xylene (x2) (Sigma Aldrich, Cat#296325) 100% ethanol (Decon ...
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bioRxiv - Biochemistry 2024Quote: ... DNP-Dpa is N-3-(2,4-dinitrophenyl)-L-2,3 diaminopropionic acid]) (Sigma Aldrich Cat. no. SCP0193-1MG) at a final concentration of 7.5 µM was added to the preincubated N-TIMP2:MMP solution ...
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bioRxiv - Neuroscience 2024Quote: ... Zebrafish larvae at 4 dpf were anaesthetised using 0.016 % ethyl 3-aminobenzoate methane sulfonate (MS-222, Sigma). They were then mounted in 1% low melting point agarose (LMP ...
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bioRxiv - Developmental Biology 2024Quote: Zebrafish larvae at 3 and 5 dpf were incubated in 2.5 ug/mL Neutral Red (Sigma-Aldrich) dissolved in E3 containing 0.003% (m/v ...
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bioRxiv - Microbiology 2024Quote: ... 3 μl of the culture was spread on BHIS plates containing 100 ng/ml of anhydrotetracycline (Sigma) for counter selection ...
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bioRxiv - Microbiology 2024Quote: ... washed 3 times with 1x phosphate-buffered saline (PBS) then mounted in Fluoroshield histology mounting medium (Sigma). For each experiment ...
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bioRxiv - Neuroscience 2024Quote: ... larvae were anesthetized with 200 mg/l 3-aminobenzoic acid ethyl ester (MS-222) (Sigma-Aldrich, A5040) and embedded laterally in 1.2% low-melting-point (LMP ...
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bioRxiv - Developmental Biology 2021Quote: PAs from homozygous a2KO (n = 5) and WT (n = 6) embryos were manually dissected in ice-cold DEPC-treated phosphate-buffered saline (DPBS, Sigma catalog number D8537). Individual PAs were pooled into four samples (WT PA1 ...
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bioRxiv - Neuroscience 2021Quote: ... 2007) was performed following a protocol described earlier with nuclei counterstained by 4’ 6-diamidino-2-phenylindole (DAPI, Sigma, D9542 10MG, 1:1000). To further assess the colocalization of different channels ...
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bioRxiv - Cell Biology 2019Quote: Cold KIF3A purification buffer (50 mM NaPi, 400 mM KCl, 2 mM MgCl2 × 6 H2O, 0.001% Brij35, 0.75 mM β-mercaptoethanol, Sigma#M3148, 0.2 mM ATP) supplemented with protease inhibitors and DNaseI was added to frozen BL21-RIL cell pellets expressing a KIF3A construct ...
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bioRxiv - Microbiology 2019Quote: ... The fixed cells were stained with 50 µg mL-1 of 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, St. Louis, MO, USA) and incubated for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated for 30 min with 10 µM 6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) and 1mg/L Hoechst 33342 dye (Sigma Aldrich, United States) diluted in media ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore, Billerica, Massachusetts, USA). The resulting 1 ml sample was loaded on top of a DG ...
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bioRxiv - Genomics 2020Quote: ... We followed the protocol as described previously [6] with one alteration: instead of using one paperdisc (Whatman qualitative filter paper, WHA1001070, SIGMA-ALDRICH CO LTD) per extraction ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... 2-(5-Benzo [1,3] dioxol-5-yl-2-tert-butyl-3HImidazol-4-yl)-6-methylpyridine hydrochloride hydrate (SB505124) (Sigma-Aldrich; St. Louis, MO) and implanted subdermally ...
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bioRxiv - Neuroscience 2021Quote: ... per well of a 6 well plate of differentiated human neural cultures (~2 months old) along with 3μg/ml of polybrene (Sigma-Aldrich #TR-1003-G), pipetting the LV concentrate directly onto the culture (drop-wise) ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... primary cortical neurons were cultured for six days in 6-well-plates followed by treatment with the mTOR inhibitor temsirolimus (10 μM in DMSO; Sigma, St. Louis, USA) or Torin 1 (500 nM in DMSO ...
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bioRxiv - Plant Biology 2020Quote: ... and their petioles dipped in 1 mM 5(6)-Carboxyfluorescein diacetate (CFDA, Ex/Em 492/517 nm, Millipore-Sigma, St. Louis, MO, USA) for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... and their petioles dipped in 1 mM 5(6)-Carboxyfluorescein diacetate (CFDA, Ex/Em 492/517 nm, Millipore-Sigma, St. Louis, MO, USA) for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: Tetracycline-inducible CALM-AF10 mouse hematopoietic progenitor cells were cultured in 15% FBS DMEM medium without IL-6 and mCSF and treated with either 4 µg/mL doxycycline (DOX) (Millipore Sigma, Billerica, MA) or DMSO for 3 Days ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred to 6 well culture dishes and rehydrated with MilliQ water and labeled in H2O for 5 min with Dapi (Sigma-Aldrich, Cat # 9542) 1:10,000 dilution of 1 mg/ml aqueous stock and Syber Gold 1:20,000 dilution of DMSO stock (ThermoFisher Cat # S11494) ...
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bioRxiv - Bioengineering 2022Quote: ... Wild type C57BL/6 mice were sacrificed and were perfused ventricularly with sodium heparin solution in PBS (H3393-100KU, Sigma-Aldrich, MO, USA). The lungs were then excised and fixed in 4% PFA overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: AIA was induced in male and female mice on a C57BL/6 background using the antigen methylated bovine serum albumin (mBSA, Sigma, St Louis, MO) as described previously26 ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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bioRxiv - Neuroscience 2019Quote: ... mice were gavaged at 4-6 weeks of age with five doses of 100 mg/kg of Tamoxifen (T5648, Sigma, St. Louis, MO) in corn oil (C8267 ...
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bioRxiv - Biophysics 2019Quote: ... Membrane was washed another 6 times in TBS-T then developed in the dark for 5 min with chemiluminescent reagent (Immobilon Forte, Millipore, Burlington, MA USA).
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bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore, Tullagreen, Carrigtwohill, IRL). The retentate was recovered and incubated with 100 µl of Micrococcus luteus peptidoglycan (1 mg/ml ...
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bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were seeded in 6-well plates (3.0 × 105 cells/well) coated with poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 24 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... female mice received subcutaneous injections (s.c.) of corn oil (CO) (25 ml/kg, vehicle control; n = 6) (#C8267-2.5L, Sigma-Aldrich, St Louise, MO, USA) or a low-dose of TCDD (20 ng/kg/d ...
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bioRxiv - Cancer Biology 2021Quote: We purchased six-week-old C57BL/6 female mice and used a reported chemical carcinogen 4-nitroquinoline 1-oxide (4-NQO, cat#N8141, Sigma, St Louis, MO) to induce ESCC as previously reported [38] ...
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bioRxiv - Bioengineering 2019Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG 2000, Laysan Lipids, Arab, AL) with 6:1:2:1 ratio in propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80 °C until all the lipids were dissolved ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were infected with the viruses in the presence of 6 μg/mL polybrene (Cat. #107689; Sigma-Aldrich, St. Louis, MO, USA). The overexpressing vectors were transfected into Ink4a/Arf-/- astrocytes using the NeonTM Transfection System 10μl Kit (Cat ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... boric acid and ascorbic acid as well as reagents required for 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) synthesis were obtained from Sigma-Aldrich (Bangalore, India). Acetone was obtained from Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Each pellet was resuspended in 500 μL (samples collected at 0 and 6 hr) or 650 μL (samples collected at 12 and 24 hr) with chilled (4°C) nuclease-free water (Sigma-Aldrich, Gillingham, UK). The resuspensions were frozen overnight at −80°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... spun down again and resuspended the cells in 200 µL of 1X PBS (phosphate buffered saline) with 1.5 μl 4′,6- diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 100 ng/ml). DAPI stains dead cells ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Microbiology 2019Quote: ... The infected HIE-18 lines were cultured in 75 cm2 flasks containing 12 ml of Schneider’s medium supplemented with 10% FBS at 28°C and subcultured every 5-6 days by splitting at a ratio of 1:2 into new flasks (Sigma Aldrich, NSW, Australia).
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bioRxiv - Molecular Biology 2021Quote: ... were myeloablated 1 day before transplant by intraperitoneal (i.p.) injection with 10 mg/kg of 6-thioguanine (6TG) (Sigma-Aldrich, Saint Louis, MO) or 35mg/kg of Busulfan for mouse m860 ...