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Citations for Millipore Sigma :
8551 - 8600 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... at 33°C for 10min (mouse brain) and 60min 75μl 0.1% pepsin (spleen) prepared at pH 2.5 in Tris-HCl (Sigma-Aldrich, USA). After a 180μl 0.1X SSC wash at 33°C ...
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bioRxiv - Immunology 2019Quote: ... and 5 μm sections were prepared and incubated with a mouse anti-human IgG4 mAb (clone MRQ-44; Sigma) or isotype control ...
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bioRxiv - Microbiology 2019Quote: ... The clarified supernatant was added to 45 μL of equilibrated mouse anti-Flag M2 affinity gel agarose (Sigma-Aldrich). The reaction was incubated overnight at 4°C on a lab rotator ...
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bioRxiv - Developmental Biology 2019Quote: ... sheared chromatin was diluted and incubated overnight at 4°C with the specific antibody against mouse Zic2 (Millipore, AB15392) or IgG isotype control pre-immune serum (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then labelled with different sets of primary and secondary antibodies: mouse anti-HA antibody (1:1000, Sigma), mouse anti-FLAG (1:1000) ...
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bioRxiv - Molecular Biology 2019Quote: ... For the first 5 days of remyelination the mice were administered 4-hydroxytamoxifen (4-HT, 1mg/day/mouse, Sigma) as previously described (Baxi ...
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bioRxiv - Neuroscience 2019Quote: ... PV (mouse anti-Parvalbumin monoclonal antibody, 1:3000, Swant) and SOM (rat-anti Somatostatin monoclonal antibody 1:250, Millipore). The following corresponding secondary antibodies were used ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with rabbit anti-mouse IgG-FITC polyclonal antibody at 1:500 (F9137, Sigma, Milan, Italy) in blocking solution at RT for 2h and counterstained with 0.5μg/mL PI at RT for 5 min ...
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bioRxiv - Physiology 2019Quote: ... Membranes were probed with the primary antibodies for puromycin (MABE343 anti-puromycin, clone 12D10 mouse monoclonal, Merck Millipore Limited), phosphorylated-4E-BP1 (Thr37/46) ...
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bioRxiv - Neuroscience 2019Quote: ... For loading control membranes were re-probed with mouse monoclonal antibody against beta-actin (clone AC-15, Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... We routinely used rabbit PLAplus and mouse PLAminus probes as secondary antibodies and the “Duolink Detection reagents Red” (Sigma) for ligation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 50 μl of mouse plasma was prepared with 450 μL of IP Lysis Buffer (Pierce) plus 0.5% protease inhibitor mixture (Sigma), and immunoprecipitated with 10 μg of polyclonal antibody against mouse ApoAI (Novus) ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was transferred onto a PVDF membrane (Immobilon-P) and detected using mouse anti-c-Myc antibody (Millipore Sigma) as a primary (1:5,000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used in this work were: mouse anti-α-tubulin clone B-512 at 1:1500 (Sigma Aldrich), mouse anti-EB1 at 1:500 (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... blotted and immunodetected with a rat anti-CLASP2 or mouse anti-α-tubulin antibodies (clone B-512, Sigma Aldrich). For analysis of recombinant CLASP2 C-term ...
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bioRxiv - Neuroscience 2019Quote: ... live mouse cortical and hippocampal neurons were incubated with rabbit anti-HA (1:1000 dilution; Sigma-Aldrich, Cat #H6908) diluted in conditioned neuronal culture medium for 15 min at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... and incubated again for 1 hour at room temperature with 1:400 mouse β-tubulin antibody (Sigma Aldrich, T5293) in PBS + 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse monoclonal antibodies to: CFTR (clone M3A7; Cat #: 05-583) and SGLT-1 (Cat #: 07-1417) were from Millipore; NKCC1 (SLC12A2 ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal loading of samples was verified by primary monoclonal mouse anti-GAPDH antibodies (clone GAPDH-71.1, Sigma-Aldrich, Belgium).
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Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: Mouse intestinal permeability was assessed by 4 kDa FITC-Dextran (FD4; Cat# 46944, Sigma-Aldrich, St. Louis, MO, USA) as previously reported 80 ...
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bioRxiv - Genomics 2019Quote: ... the blocking solution was aspirated and primary antibodies were added (mouse anti-GFP antibodies [Sigma] at 1:100 dilution). After incubation for 1 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: Rds2-FLAG was immunoprecipitated from glucose-treated and non-treated cultures using anti-FLAG M2 antibody (mouse mAb, Sigma) and eluted by FLAG peptide elution ...
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bioRxiv - Bioengineering 2019Quote: ... and either α-smooth muscle actin (α-SMA, mouse monoclonal anti-α-SMA clone 1A4, 1:400, Sigma-Aldrich) or rhodamine phalloidin to visualize F-actin (1:600 ...
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bioRxiv - Genomics 2021Quote: ... S9.6-treated or anti-FLAG treated cells were incubated with 1:100 dilution of anti-mouse antibody (Sigma, M7023) in 200µl of dig-wash buffer at room temperature for 1 hour by rotating.
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with peroxidase-coupled secondary IgG antibodies (anti-Rabbit–A0545 or anti-Mouse –A9044, Sigma Aldrich) for 2 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Antibodies were purified as described below or purchased from the following manufacturers: Mouse anti-FLAG M2 (Sigma-Aldrich; F3165); Goat anti-Mouse IgG(H+L) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were further incubated for two nights at 4°C with mouse ACTA2-FITC antibodies (1:200; Sigma;F3777) in blocking buffer containing 1 to 5% bovine serum albumin in 0.25–0.5% Triton-X 100 in 1× PBS ...
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bioRxiv - Neuroscience 2020Quote: Mice were transferred onto a diet of crushed mouse food (Barrestock) containing 0.2% (w/w) cuprizone powder (C9012, Sigma), which was refreshed every 2 days for 5 weeks ...
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bioRxiv - Biochemistry 2021Quote: Antibodies and reagents used for immunoprecipitation and western blotting include mouse anti-FLAG M2 agarose affinity gel (Sigma-Aldrich), mouse anti-DYKDDDDK (Flag ...
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bioRxiv - Bioengineering 2020Quote: ... Three sections at varying depths within the tumor are immunostained with mouse anti-human nuclei (clone 235-1, Millipore) followed by secondary Dylight 488 horse anti-mouse (Vector) ...
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bioRxiv - Neuroscience 2021Quote: ... Pan neuronal staining was also processed (monoclonal mouse antibodies, 1:500, Neuro-ChromTM Pan Neuronal Marker Millipore MAB 2300) and revealed with a goat antimouse antibody conjugated to Alexa 568 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies (Anti-Alpha-synuclein - phospho S129, rabbit polyclonal, Abcam, Cambridge, UK; Anti-GAD67, mouse monoclonal, Millipore, CA, USA) were incubated for 48h at 4°C in a 1:1000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 10% bovine serum albumin (BSA) 30 min at 37°C and incubated with primary anti-γH2A.X mouse antibodies (Merck Millipore) or rabbit anti-LC3B antibodies (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with appropriate biotinylated secondary antibodies at 1:500 (anti-mouse, Millipore AP124B or anti-Millipore AP132B), followed by Vector ABC standard detection kit (Vector Laboratories PK-6100) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with appropriate biotinylated secondary antibodies at 1:500 (anti-mouse, Millipore AP124B or anti-Millipore AP132B), followed by Vector ABC standard detection kit (Vector Laboratories PK-6100) ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to nitrocellulose membrane and probed with primary antibodies against HA (HA-7 mouse monoclonal, 1:2000 dilution, Sigma; or rabbit polyclonal ...
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bioRxiv - Physiology 2020Quote: ... and subsequently used for immunoprecipitations (IP) with 48 μg of an anti-NaVPAN mouse monoclonal antibody (mαNaVPAN, Sigma, #S8809), raised against the SP19 epitope (45 ...
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bioRxiv - Bioengineering 2021Quote: ... diluted in PBS for 15 min and then incubated with 1:500 mouse anti-α-tubulin antibody (Sigma, T6199) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... For all ChIP experiments a mock IP and an experimental IP were set up at the same time using 1,350 µl pre-cleared chromatin and 10 µg of normal mouse IgG (Millipore) and monoclonal mouse anti-c-Myc respectively ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then labeled with a mouse monoclonal antibody to complement components C3b and iC3b (Clone 3E7; Sigma-Aldrich) diluted 1:100 in blocking solution at 4°C overnight on a rocker ...
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bioRxiv - Neuroscience 2020Quote: ... were performed prior to primary antibody incubation (12hrs, biotinylated mouse monoclonal antibody, Sigma-Aldrich SIG-39240 Beta-Amyloid - 4G8) at 2μg/ml (antibody diluent ...
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bioRxiv - Neuroscience 2020Quote: ... Both immunized and SHAM mice were administered with Pertussis toxin (400 ng/mouse, Sigma-Aldrich, St. Louis, MO, USA), injected intravenously through the tail vein on the day of immunization and 48 h later ...
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bioRxiv - Genomics 2021Quote: ... Cells were pelleted again and resuspended in 50 µL of permeabilization buffer with 2% mouse serum (Millipore Sigma, M5905) to block for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... separated by SDS-PAGE electrophoresis and knockdown was analyzed by Western blotting using mouse anti-HA (1:1000; Sigma) antibody with rabbit anti-EXP2 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse myoblast C2C12 cells (gift from Edgar R. Gomis, iMM, Portugal) were grown in DMEM High Glucose (Sigma Aldrich) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated with 381.7 ng / ml mouse α-HAdV hexon protein antibody (Mab8052, Sigma-Aldrich, St. Louis, USA) and subsequently stained using 2 μg / ml goat α-mouse-AlexaFluor594 (A21203 or A32742 ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... primary mouse monoclonal anti-β-tubulin antibody (T8328) and all ammonium and sodium salts were purchased from Sigma-Aldrich GmbH (Taufkirchen ...
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bioRxiv - Cell Biology 2020Quote: ... The following PLA probes were used in this work: Duolink In Situ PLA Probe Anti-Mouse Minus (SIGMA, DUO9004); Duolink In Situ PLA Probe Anti-Mouse Plus (SIGMA ...