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Citations for Millipore Sigma :
8501 - 8550 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... and incubated for 2 h in 1:20,000 horseradish peroxidase (HRP)-conjugated goat α-mouse IgG (Sigma-Aldrich)/1% BSA/TBS ...
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bioRxiv - Pathology 2024Quote: ... 0.5% Triton and lysates were incubated with rabbit or mouse isotypic control antibodies and G-protein beads (Sigma) for 1 hr at 4°C ...
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bioRxiv - Immunology 2022Quote: ... the total DNA methylation signature was evaluated in 100 ng of DNA using the commercial kit (Imprint Methylated DNA Quantification kit, Sigma-Aldrich), according to the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2020Quote: ... Apoptosis was assessed by DNA fragmentation staining using a commercially available kit (In Situ Cell Death Detection Kit, TMR red, Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL ...
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bioRxiv - Microbiology 2020Quote: For the measurement of proteasome activity a fluorometric assay kit (Proteasome 20S Activity Assay kit) that measures the chymotrypsin-like protease activity was used following manufactured instructions (Sigma-Aldrich). A total of 100 µg of protein extracts were incubated with LLVY-R110 substrate provided by the kit for 2 h at 30°C in dark ...
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bioRxiv - Microbiology 2022Quote: Aldehyde and D-lactate production in Salmonella strains were measured by the Aldehyde Assay Kit and D-Lactate Assay Kit (Sigma-Aldrich), respectively ...
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bioRxiv - Cell Biology 2022Quote: RNA from different cancer cell lines was extracted using RNA isolation kit (Bio Rad) and treated with DNAse using DNAse 1 kit (AMPD1, Sigma-Aldrich). cDNA synthesis was done using a cDNA synthesis kit (Bio Rad) ...
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bioRxiv - Synthetic Biology 2020Quote: ... the intracellular ATP were measured using the enzymatic assay kit (ATP Bioluminescence Asssay Kit HS II, Sigma No. 11699709001, Roche, Switzerland) after the cell disruption of the corynebacterial strains ...
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bioRxiv - Zoology 2020Quote: ... DNA was extracted from individual cysts or juveniles using the “Sigma Extract-N-Amp Kit (XNAT2)” kit (Sigma, St. Louis, MO) according to the protocol reported by Ma et al ...
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bioRxiv - Microbiology 2022Quote: ... Cells were verified using the Promega GenePrint STR kit (DNA Analysis Facility, Duke University) and as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated and converted into cDNA using a commercially available kit (GenElute Mammalian Total RNA Miniprep Kit; Sigma-Aldrich, USA). Transcript levels were determined by real-time quantitative polymerase chain reaction (QuantStudio 5 ...
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bioRxiv - Evolutionary Biology 2024Quote: Random-PCR amplification of the Viral DNA or RNA were respectively achieved with the GenomePlex Complete Whole Genome Amplification (WGA) Kit and the TransPlex Whole Transcriptome Amplification (WTA) Kit (Sigma-Aldrich) according the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... All cells were regularly tested for the presence of mycoplasma using the PCR Mycoplasm Test Kit (PromoKine) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... Cell proliferation was determined with the iClick EdU Imaging Kit (ABP Biosciences) and apoptosis was measured using the ApopTag Peroxidase in Situ Apoptosis Detection Kit (Millipore Sigma), following the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 100% for 30 minutes each) and then embedded in epoxy embedding medium kit (Epon substitute embedding medium kit; 45359, Sigma Aldrich). For this ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: ... Mix-n-Stain CF 555 Antibody Labeling Kit and Mix-n-Stain CF 647 Antibody Labeling Kit (Sigma-Aldrich, MX555S100 and MX647S100) were used according to the manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... an Alexa Fluor™ 568 antibody labeling kit and a Mix-n-Stain™ CF®633 Dye antibody labeling kit (Sigma) were used according to manufacturer’s protocols.
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bioRxiv - Neuroscience 2020Quote: Primary cultures fixed and permeabilised as described for IF were used to perform PLA using a commercial kit (Duolink® PLA starter kit, Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA of control and insertion lines was extracted using a commercial kit (Spectrum™ Plant Total RNA Kit, Sigma life science) following the supplier’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lines were routinely tested negative for mycoplasma contamination using Universal Mycoplasma detection kit (ATCC, 30-1012K) or LookOut Mycoplasma Detection Kit (Sigma-Aldrich, MP0035). The INF2-KO U2OS cell line made by CRISPR-Cas9 is described elsewhere (Chakrabarti et al. ...
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bioRxiv - Developmental Biology 2022Quote: An ATP calibration curve was performed using the stabilized ATP standard stocks provided in the kit ATP Bioluminescence Assay Kit HS II (Millipore Sigma #11699709001) and following the supplied protocol ...
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Induction of osteogenic differentiation of MSCs by GSK3β knockdown through GSK3β siRNAs transfectionbioRxiv - Molecular Biology 2023Quote: Activity of ALPL and calcium ions concentration in cell lysates on day 7 of osteogenic differentiation were determined using reagent kits “Alkaline Phosphatase-New Liquid Form” (Vector-Best, Russia) and Calcium Colorimetric Assay Kit (Sigma-Aldrich, USA), respectively ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted using Qiagen RNeasy Mini Kit (Maryland, USA) according to the manufacturer’s protocol and DNase-treated using the DNase I kit (Millipore Sigma, Massachusetts, USA). The quantity and quality of RNA were determined using the Nanodrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... were incubated for 45 min at 4°C with 10 µL agarose beads using a RAS-GTP pull-down assay kit (RAS Activation Assay Kit, Merck Millipore, 17-218). Supernatant was recovered after centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-mouse RIPK3 (clone 8G7; produced in-house5 and available from Millipore as MABC1595; 1:2000/1:400), rat anti-mouse RIPK3 (clone 1H12 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532 ...
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bioRxiv - Cell Biology 2020Quote: Proteins tagged with HA (Mps2-3HA and 3HA-Mps2) were detected by an anti-HA mouse monoclonal antibody (1:1,000 dilution, 12CA5; Sigma). Similarly ...
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bioRxiv - Cell Biology 2020Quote: The following commercial primary antibodies were used throughout this study: Mouse monoclonal antibodies against Actin (clone C4 MAB1501R, Millipore), Fibrillarin (ab18380 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-α-tubulin (1:200 for IF and 1:2500 or 1:10,000 for IB; Sigma-Aldrich); rabbit polyclonal anti-CALK (1:10000 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-Flag used at 1:5,000 (Western blot) and mouse anti-tubulin used at 1:5,000 (Western blot) were obtained from Sigma-Aldrich. Mouse anti-GFP used at 1:100 (immunofluorescence ...
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Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis IIbioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-PP2A C subunit clone 1D6 Alexa Fluor 488 conjugate (Sigma-Aldrich, 05-421-AF488, 1:50), polyclonal rabbit anti-Sgo2 antibody (gift from José Luis Barbero ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA for genotyping was isolated from mouse tail snips with lysis buffer containing Proteinase K (Sigma-Aldrich, P2308). qPCR was performed using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Immunology 2021Quote: ... The same volumes of PBS and LPS (20 μg/mouse, from E. coli O114:B4, Sigma-Aldrich, Shanghai, China) were used as negative control and positive control ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-Neurofilament (cat. #RT97, DSHB, 1:200) nuclei were labeled with DAPI (cat. #D9542, Sigma Aldrich, 1:1000). Images were captured using LSM 510 META ...
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bioRxiv - Developmental Biology 2021Quote: Whole embryonic mouse brains were harvested at E15.5 and lysed in NP-40 lysis buffer with protease inhibitor cocktail (Sigma). Whole brain lysates from one embryo were added to either 6μg GST or 6μg GST-MLLT11 bead slurries and nutated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... The antigen-antibody complexes were visualized using a secondary horseradish peroxidase-conjugated goat anti-mouse IgM antibody (Sigma-Aldrich) at a dilution of 1:10,000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were stained with anti-Rhodopsin rabbit and mouse antibodies (1:200, home-made and 1:200, Millipore, #MABN15), anti-Zpr1 mouse antibody (1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... Several antibodies were used in this study (dilutions are between brackets): Mouse IgG1 anti-Brn3a (Millipore Cat# MAB1585 RRID:AB_94166) (1:250) ...
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bioRxiv - Neuroscience 2022Quote: The resulting concentrate was analyzed using the Milliplex 32-plex Mouse Cytokine/Chemokine Panel (Millipore Sigma MCYTMAG-70K-Px32) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
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bioRxiv - Neuroscience 2021Quote: ... Kyoto University) and mouse anti-glutamic acid decarboxylase of 67 kDa (GAD67; 2 μg/mL; MAB5406, Millipore, RRID: AB_2278725) in Triton-PBS containing 1% v/v donkey serum (Jackson Immunoresearch ...
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bioRxiv - Molecular Biology 2021Quote: Hamster and mouse tissues were homogenized mechanically in RIPA lysis buffer supplemented with 1x protease inhibitor cocktail set (Millipore) and loaded with SDS dye ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with 5% milk in PBS-T and then blotted with a mouse anti-HTT (MAB2166. dilution: 1:5,000; Millipore), rabbit anti β--catenin (Ab2365 ...
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bioRxiv - Immunology 2022Quote: ... The affinity Sepharose resin was prepared by coupling purified rat anti-mouse YN1.1 antibody with CNBr-activated Sepharose 4B (Sigma).