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Citations for Millipore Sigma :
8501 - 8550 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... cell surface protein samples were concentrated into 0.1 ml volumes using a 30 kDa centrifugal concentrator (Merck Millipore).
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bioRxiv - Plant Biology 2024Quote: ... GST-fusion proteins were purified by loading total bacterial lysate on a preequilibrated glutathione Sepharose column (Sigma, Germany) using fast performance liquid chromatography system (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 mg TTN5 protein was combined with 1 U of agarose bead-coupled alkaline phosphatase (Sigma Aldrich, Germany) for degradation of bound GDP to GMP and Pi in the presence of 1.5-fold molar excess of non-hydrolyzable GTP analog GppCp (Jena Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... Protein was then transferred to a methanol soaked polyvinylidene difluoride (PVDF) membrane (Millipore Immobilon FL 0.45 µm #IPFL00010). The membrane was then PBS washed ...
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bioRxiv - Physiology 2024Quote: ... Total protein extracts were obtained by transferring the bronchioids to Dulbecco′s phosphate-buffered saline (DPBS, Sigma-Aldrich) for 30 minutes at 37 °C with gentle agitation to dissolve the alginate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... expression of the S protein was induced by the addition of 50 ng/ml dox (Sigma-Aldrich, D9891) for 24 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein extracts were fractioned on SDS-PAGE and examined by Western blot with mouse anti-myc (Sigma), mouse anti-Pgk1 (Thermofisher) ...
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bioRxiv - Biophysics 2024Quote: ... Oligomeric α-Syn species were removed by filtering the protein through a 50-kD cutoff column (Amicon, Millipore). Aliquots of the protein were prepared ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted from the beads by adding 25 μL of Laemli Sample Buffer 2X (Sigma-Aldrich, #S3401), before heating tubes at 95°C ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved protein was collected and concentrated using 100 kDa MW cut-off spin concentrators (Amicon Merck-Millipore) separated by size-exclusion chromatography (SEC) ...
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bioRxiv - Microbiology 2024Quote: ... Samples normalized for the protein content were separated by SDS-PAGE and transferred onto a PVDF membrane (Millipore). After washing with TBS buffer twice ...
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bioRxiv - Cancer Biology 2024Quote: ... 10-50 μg total protein was fractionated by SDS-PAGE and transferred to PVDF or nitrocellulose membranes (Millipore). Blocking was achieved with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein fractions were pooled and concentrated using a 10 kDa cut-off (Amicon filter, Millipore, USA). After affinity purification ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentrations of the cell lysate or EV pellets were quantified with Pierce 660 nm reagent (Sigma #22660) using the manufacturer’s protocol and comparing to a bovine serum albumin standard curve.
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bioRxiv - Cancer Biology 2024Quote: ... Proliferating AT2 cells were identified by immunofluorescence for the AT2 marker surfactant protein-C (SPC, EMD Millipore, #AB3786) and proliferation maker Ki67 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Protein was eluted from Sepharose A by addition of 35 μL sample loading buffer (Sigma-Aldrich, S3401-10) and incubation at 80 °C for 5 min ...
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bioRxiv - Microbiology 2024Quote: Fifteen amino acid (aa) peptides overlapping 12 aa spanning the structural proteins of CHIKV were purchased from Sigma and were divided into 4 overlapping peptide pools ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and 10 µl of total protein extract was added to 100 µM nitrocefin (Sigma-Aldrich) solution in PIPES 2 mM pH 6.8 and substrate hydrolysis measured spectrophotometrically over 30 minutes (Cytation5 ...
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bioRxiv - Molecular Biology 2024Quote: ... For dTAG-13 induced protein degradation cells were treated with 500 nM dTAG-13 (Sigma-Aldrich, SML2601-5MG) or an equivalent volume of DMSO (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: ... 0.5% Triton and lysates were incubated with rabbit or mouse isotypic control antibodies and G-protein beads (Sigma) for 1 hr at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... co-staining was performed using rabbit anti-perilipin A antibody as an adipocyte marker in combination with a mouse anti-human mitochondria (hMito) antibody conjugated with Cy3 (EMD Millipore, Burlington, USA; 1:100 dilution). hMito was used as an alternative to Ku80 based on the availability of antibodies compatible for co-staining ...
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bioRxiv - Cancer Biology 2021Quote: ... ERK5 was silenced with human or mouse shRNAs purchased as pre-cloned in the pLKO.1-puro plasmid (MISSION® shRNA, Sigma-Aldrich, St Louis, MO, USA). Control shRNAs in the same plasmid were also purchased (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... transferrin from human serum, (Tf), cytosine (Cyt), trifluoroacetic acid (TFA, ≥99%), and formic acid (FA, analytical grade >98%) were purchased from Sigma Aldrich (St. Louis, Missouri, United States). Methanol (MeOH) ...
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bioRxiv - Immunology 2021Quote: ... secondary antibody (goat anti-human γ-chain-specific whole IgG, α-chain-specific IgA, or μ-chain-specific IgM alkaline phosphatase conjugate, Sigma Aldrich, MO, US) was diluted 1:1000 and 50μl was added per well for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... the autoclaved peg lid was incubated in a flat-bottom 96-well plate with 200 µl of 100 µg/ml fibrinogen from human plasma (product number F3879, Sigma-Aldrich, in 0.9% NaCl or PBS) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells were transfected as described above with empty or human SLFN14 expression plasmids and a plasmid encoding Hluc in the presence of RNA Pol III inhibitor (Sigma ML-60218, 40 and 20 uM) or tofacitinib (Sigma PZ0017 ...
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bioRxiv - Cancer Biology 2023Quote: ... and HPCD-3BP were incubated at various concentrations (12.5 μM, 25 μM, 50 μM, 100 μM, and 200 μM) with 90 μL of human serum (Sigma, Cat No. H3667; St. Louis, MO, USA) at 37 °C for up to 8 h ...
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in matched BAL fluid and plasma collected from patients were measured using the multiplexed human cytokine/chemokine magnetic bead kits from Millipore (HCYTMAG-60K-PX41 and HCYP2MAG-62K) according to manufacturer’s protocol (HCYTOMAG-60K Rev ...
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bioRxiv - Microbiology 2020Quote: ... Collected cells were immediately spun down and resuspended in BugBuster® Protein Extraction reagent (Novagen, Merck and Co. Inc) to lyse the bacterial cells and release proteins ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by 10% SDS-PAGE gel and transferred to 0.45 µm polyvinylidene difluoride (PVDF) membrane (Millipore, USA). After blocking by with 5% non-fat dry milk for 2h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride membranes (Millipore). After in-cubated with antibodies ...
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bioRxiv - Cell Biology 2019Quote: Protein was extracted from isolated cell pellets or whole liver tissue using RIPA buffer with protease inhibitors (Sigma-Aldrich). Equal amounts of protein were loaded and separated by SDS-PAGE gel electrophoresis on 4%-20% Criterion gels (BioRad ...
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bioRxiv - Cell Biology 2019Quote: ... the extracted proteins were separated in 10% polyacrylamide gels (Schägger and von Jagow, 1987) and blotted onto Immobilion-Fl membranes (Millipore). The membranes were incubated in blocking solution (5% skim milk in TBST ...
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bioRxiv - Cell Biology 2019Quote: ... cells were serum starved for 48 hours and proteins were extracted with lysis buffer (Sigma Cell Lytic Solution C2978) with 0.1% CHAPSO ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein of cell extracts was determined by Bradford method and GST□BHMT was precipitated with glutathione agarose (Sigma). Precipitates were then mixed in SDS□PAGE sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Microbiology 2020Quote: ... The mitochondrial import assay results were analysed by visualizing the proteins with the Coomassie stain (PhastGel Blue R, Sigma). The proteins were then fixed (20% ethanol and 10% acetic acid) ...
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bioRxiv - Microbiology 2020Quote: ... 30 μg of total protein was analyzed by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (Millipore). The membrane was probed with antibodies at 4 °C overnight and visualized using Western Lightning™ Plus ECL (erkinElmer ...
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bioRxiv - Molecular Biology 2021Quote: Western blot analysis of protein levels was performed on whole cell lysates prepared by lysis in RIPA buffer (Sigma) supplemented with 1 mM DTT (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing Exo1 protein variant were pooled and applied to 0.3 mL of M2 anti-FLAG agarose beads (Sigma) in batch ...
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bioRxiv - Developmental Biology 2021Quote: ... The lysate was centrifuged to remove cell debris and then incubated with Protein G Sepharose Fast Flow (Sigma-Aldrich) for 1 h at 4°C to remove nonspecifically bound proteins ...
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bioRxiv - Genomics 2021Quote: ... Antibodies-chromatin complexes were then pulled-down using 20 μL Magna ChIP™ protein A+G magnetic beads (Millipore) by incubating with rotation (20 rpm ...
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bioRxiv - Microbiology 2021Quote: ... Sized proteins were brought to concentrations typically >50 mg mL−1 using appropriate molecular weight cut-off concentrators (Millipore), flash frozen on liquid nitrogen ...