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Citations for Millipore Sigma :
8451 - 8500 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: mAb TnI-1 (IgG1) was purified from mouse hybridoma ascites fluid using Protein G chromatography (Millipore Sigma P7700). TnI-1 IgG eluted with 50 mM glycine-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... The expression of the proteins was assessed by western blot using antibodies raised against the T7 epitope (Novagen) and GAPDH (Abcam).
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bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing the protein were pooled and concentrated in centrifugal concentrators (CLVG01230, Merck Millipore, Cork, Ireland) to a final concentration of 30 mg/ml for crystallization ...
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bioRxiv - Biophysics 2023Quote: ... Protein degradation was induced by treating cells with a final concentration of 400 µM dTAG-13 (Sigma SML2601) for 2 hrs or 6 hrs (2 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... N-glycosylated proteins were enriched by incubating cell lysates containing protease inhibitor and 1,10-phenanthroline (Sigma-Aldrich, #131377) with 20µl concanavalin A Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... HIV-Tat protein transduction motif (YGRKKRRQRRR) was subcloned into a pET-46 Ek/LIC vector (EMD Millipore #71335). The construct encoding a GST-tagged caltubin was subcloned into a pGEX-4T-1 vector (Amersham #28954549) ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were separated on GenScript SurePAGETM Bis-Tris gels and blotted on the Immobilon-P transfer membrane (Millipore). The membrane was blocked with 5% skim milk and incubated with primary antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were run on NuPAGE 4%–12% Bis-Tris Gel (Novex) and transferred onto PVDF membranes (Millipore Sigma) and probed with antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenate of 50 μg was added to a process control of 800 ng of yeast enolase protein (Sigma) which was then reduced with 20 mM DTT and alkylated with 40 mM IAA ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were filter-concentrated using centrifugation and a 10-kDa or 30-kDa cut-off column (Millipore Sigma) and dialyzed at 4 °C for ∼24 h against Dialysis Buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were pre-cleared for 1 h with protein A Sepharose CL-4B (Sigma-Aldrich, 17-0780-01) beads blocked with 0.2 µg/µl sonicated herring sperm DNA (Thermo Fisher ...
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Characterization of an evolutionarily distinct bacterial ceramide kinase from Caulobacter crescentusbioRxiv - Biochemistry 2023Quote: ... The dialyzed protein was concentrated using an Amicon Ultra centrifugal filter (10 kDa molecular weight cutoff) (Millipore Sigma). The protein concentration was determined using the BCA Protein Assay Kit (Pierce).
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were transferred into 100 kilodalton (kDa) molecular weight cut-off (MWCO) protein concentrators (Millipore-Sigma, UFC 805024) and concentrated from 5 ml to 0.5 ml ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 1mg protein lysate was incubated with 50 µl suspension of monoclonal anti-HA agarose (Millipore). After overnight incubation ...
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bioRxiv - Immunology 2023Quote: ... The protein was further purified by anion exchange chromatography using a SOURCE™ 15 Q column (MILLIPORE SIGMA) and eluted in the final protein buffer containing 20mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... Purified proteins were concentrated to >15 mg ml−1 using 30-kDa MWCO centrifugal filter units (Millipore Sigma), aliquoted ...
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bioRxiv - Microbiology 2023Quote: ... Purified proteins were concentrated to >10 mg mL−1 using a 30 kDa MWCO centrifugal filter (Millipore Sigma), aliquoted ...
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bioRxiv - Genomics 2023Quote: ... (b) Reduce background signal by pre-clearing with 20µL of Protein A/G magnetic beads (Millipore 16-663) per 1mL of sample for 2 hours at 4°C with rotation ...
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bioRxiv - Biochemistry 2023Quote: ... The GCL reaction was stopped by precipitation of proteins with 200 mM 5-sulfosalicylic acid (Sigma Aldrich, 247006) and then put onto ice for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were transferred into 100 kilodalton (kDa) molecular weight cut-off (MWCO) protein concentrators (Millipore-Sigma, UFC 805024) and concentrated from 5 ml to 0.5 ml ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleic acids from the protein samples were removed by incubating the samples with Benzonase (Millipore Sigma, Cat. No.E1014) for 20 min on ice before use in Sun1 interacting pulldown assay.
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bioRxiv - Microbiology 2023Quote: ... Enriched proteins were denatured in U/T buffer (6 M urea, 2 M thiourea, 1 mM DTT (Sigma), 10 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: Protein isolation was performed by lysis of the cells in RIPA buffer (Sigma-Aldrich, St. Louis, United States), supplemented with complete protease and phosphatase inhibitors (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... The nuclear protein lysate then was pre-cleared by adding 2 μL of poly D[IC] (Sigma-Aldrich) 25 µg/µL and 20 μL of High Capacity Strepavidin agarose beads (Thermofisher #20357) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total protein was quantified following the method described by Bradford using bovine serum albumin (BSA, Sigma cat. 7906) as standard ...
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bioRxiv - Biochemistry 2024Quote: ... Each reaction mixture constituted protein at the desired concentration with 1.25 mM Tris (2,2’-bipyridyl)dichlororuthenium(II)hexahydrate (Sigma, CAS No ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were expressed in the BL21 strain from Escherichia coli at 37°C in LB medium (Sigma) supplemented with 100 µg/mL ampicillin until OD600 between 0.4 and 0.5 ...
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bioRxiv - Neuroscience 2024Quote: 10 µg of protein was loaded per sample and membrane was probed with antibodies against APOE (Sigma; AB947), RHEB (Abcam ...
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bioRxiv - Physiology 2024Quote: ... The protein samples were separated by 10% SDS-PAGE and then transferred to polyvinylidene fluoride membranes (Millipore, USA). The membranes were blocked with 10% non-fat dry milk in Tris-buffered saline with Tween-20 for 1 h at room temperature and then incubated with primary antibodies overnight at 4℃ (anti-phospho-PKA ...
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bioRxiv - Neuroscience 2023Quote: Total protein from BFP expressing iNeurons was extracted using CelLytic™ M Cell Lysis Reagent (Sigma-Aldrich, #C2978) with PhosSTOP™ (Roche ...
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bioRxiv - Biophysics 2023Quote: Plasmids for analyte proteins were constructed with gBlocks (Integrated DNA Technologies) inserted into the pET-49b(+) plasmid (Novagen), with a dihydrofolate reductase domain ...
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bioRxiv - Biophysics 2023Quote: ... The purified protein (in 25 mM piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES, P-1851, Sigma-Aldrich) pH 6.8 ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins was performed with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, cat.no. A2220-1ML) at 4 °C with overhead rotation for 6 h ...
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bioRxiv - Cell Biology 2023Quote: ... The pure proteins were concentrated with an Amicon Ultra-15 centrifugal filter (10 kDa MWCO, Merk Millipore Inc.) to around 20 mg/mL.
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 5 mL per gram of cells of a hypotonic solution (5 mM MgCl2, 0.25 mg/mL lysozyme from chicken egg white >40,000 units/mg protein (Sigma) and 1 mM phenylmethylsulfonyl fluoride) ...
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bioRxiv - Neuroscience 2023Quote: ... the primary antibody was glial fibrillary acidic protein (GFAP) (1:1000 dilution; Millipore, Temecula, CA, USA; Cat. #MAB360). Secondary antibodies were conjugated to Alexa Fluor 594 (donkey anti-mouse ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged protein was eluted off the beads by using 20 mM glutathione solution (Sigma-Aldrich, #G4251-5G). Fractions were analyzed by resolving on 4-20 % gradient SDS-PAGE (BioRAD #4561094 or #4651093) ...
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bioRxiv - Developmental Biology 2023Quote: ... The proteins bound to the beads were reduced with 10 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma, C4706) at 40°C for 30 min and alkylated with 20 mM Iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
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bioRxiv - Cell Biology 2023Quote: ... The fractions corresponding to the protein complexes were concentrated using a 500-μl 30K MWCO concentrator (Millipore-Sigma).
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bioRxiv - Cell Biology 2023Quote: ... The fractions corresponding to the protein complexes were concentrated using a 500-μl 30K MWCO concentrator (Millipore-Sigma).
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bioRxiv - Biochemistry 2024Quote: ... appropriate AGO:RNA bands were excised from the gel and protein digested with Proteinase K (RPROTK-RO, Sigma Aldrich) and released RNA isolated via phenol:chloroform phase separation ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were extracted from the residue debris pellets and quantified using a BCA kit (Sigma-Aldrich, Taufkirchen, Germany). Lipid profiling was performed using a Sciex ExionLC AD coupled to a Sciex ZenoTOF 7600 under control of Sciex OS 3.0 (Sciex ...
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bioRxiv - Neuroscience 2023Quote: ... CopiaGag capsid protein was concentrated using a 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit(EMD Millipore) to a final concentration of 0.8 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... that contained protein: carbohydrate: fat (20:20:60, kcal%) plus high fructose (23.1 g/L; F3510, Sigma,USA) and glucose (18.9 g/L ...
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bioRxiv - Genetics 2024Quote: ... complex was prepared 15 min before transfection by mixing gRNAs (1.5 µM) with Cas9 protein (0.45 µM) (Sigma, Catalog no ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fractions containing the protein were pooled and concentrated with a 30 kDa cutoff Amicon centrifugal filter (Sigma UFC9030) and loaded onto a Superdex 200 size exclusion column equilibrated with the storage buffer (25mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... 10-30 μg of protein was resolved by electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore). Membranes were blocked in TRIS-buffered saline with 0.1% tween 20 and 5% BSA for 1 hour and then incubated overnight with primary antibody at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of total protein were loaded onto an SDS-PAGE before transfer onto a nitrocellulose membrane (Millipore). The membranes were blocked in 5% (w/v ...