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Citations for Millipore Sigma :
8501 - 8550 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... in a 10-cm dish transfected one day post seeding with packaging plasmids (1 μg VSV.G, 2 μg psPAX2 and 4 μg of transfer plasmid using PEI (Sigma-Aldrich). Virus containing supernatant was collected 72 hours post transfection ...
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bioRxiv - Immunology 2020Quote: ... siLP cells were released by digestion of the tissue with RPMI/4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) supplemented with 60 μg/ml DNaseI (Sigma), and 400 ng/ml of Liberase (Roche Applied Science ...
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bioRxiv - Synthetic Biology 2020Quote: Enriched M9 media was prepared using M9 salts (Difco M9 Minimal Salts) supplemented with 2 mmol L−1 magnesium sulfate (Sigma), 100 μmol L−1 calcium chloride (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... wells were washed with embryo media before the fish were incubated in media containing 0.01% 2-[4-(dimethylamino) styryl]-1-ethylpyridinium iodide (DASPEI, Sigma-Aldrich) to stain for neuromast mitochondrial activity for 20 minutes ...
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bioRxiv - Cancer Biology 2021Quote: For stable knock down of PYCR1, shPYCR1 (shPCYR1 #1: CCGGTGAGAAGAA GCTGTCAGCGTTCTCGAGAACGCTGACAGCTTCTTCTCATTTTTG, shPYCR1 #2: CCGGCACAGTTTCTGC TCTCAGGAACTCGAGTTCCTGAGAGCAGAAACTGTGTTTTTG) and shCTL (Sigma, Mission shRNA) lentivirus was generated in HEK293 cells ...
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bioRxiv - Immunology 2021Quote: ... 106 cells per sample were cultured in RPMI containing 2% FBS and stimulated with PMA (50 ng ml−1, Sigma), ionomycin (2 μg ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was re-suspended in ice-cold 100 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid) buffer (pH 8.0) containing complete protease inhibitor cocktail (Sigma, USA) and PMSF (phenylmethanesulphonyl fluoride ...
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bioRxiv - Cell Biology 2020Quote: Imaging was performed on live animals mounted on a 2% agarose pad on glass slides with 1 mM levamisole (Sigma). For quantification of purified mitochondria and mitochondrial membrane potential ...
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bioRxiv - Bioengineering 2020Quote: ... were added to desalted (GT)15 oligonucleotide (2 mg, Integrated DNA Technologies) in a microcentrifuge tube with NaCl solution (1 mL, 0.1 M, Sigma-Aldrich). The mixture was then ultrasonicated using a 1/8″ tapered microtip (Sonics Vibracell ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were fed with a continuous flow at 1 Psi of HS media containing 2% glucose and 0.001% Fluorescence Brighter 28 (Sigma-Aldrich). After 24 hours ...
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bioRxiv - Immunology 2020Quote: ... from 1:50 to 1:51200 in PBS containing 2% skimmed milk were added followed by ALP-conjugated anti-human IgG (A9544; Sigma) at 1:10,000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... and HCT116 cells with acquired BOLD-100/KP1339 resistance were seeded as 2 × 105 cells/well in 12-well plate formate in 1 mL McCoy’s medium (Sigma Aldrich) supplemented with 2 mm glutamine and 10% FCS ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown to early log phase in synthetic complete (SC) media + 2% glycerol + 1% ethanol and gently sonicated before being loaded into a CellASIC Y04C microfluidics plate (Milipore SIGMA) under continuous media flow at 2 psi ...
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bioRxiv - Neuroscience 2020Quote: ... blocked in normal donkey serum in PBST (2%) and transferred to rabbit anti-Fos primary antibody (1:10000; EMD Millipore) in PBST and normal donkey serum for 16 hr at RT ...
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bioRxiv - Microbiology 2020Quote: Whole cell protein fractions were prepared by centrifuging 1 ml of overnight bacterial culture and resuspending the cell pellet in 2 x Laemmli sample buffer (Sigma) and boiled for 5 min at 100°C ...
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bioRxiv - Plant Biology 2020Quote: ... Purity of the purified protein was controlled on SDS-PAGE and it was brought to a final concentration of 2 mg mL-1 using an Amicon-Ultra device (Millipore). Polyclonal antibodies against FAP were raised in rabbits (ProteoGenix ...
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bioRxiv - Microbiology 2020Quote: ... Residual methylation reagents were neutralised by addition of 1M Tris-HCl pH 7.5 to a final concentration of 50 mM Tris-HCl and the protein complex was concentrated to 2 mg mL−1 using 30 kDa cut-off concentrators (Millipore) before injection into a S75 16/600 column (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... coli strain and cultured the cells in M9 minimal medium (Difco) supplemented with 1/2 MEM amino acids solution (SIGMA) and 0.2% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... then diluted to 1×107 cells/ml in liquid YPAD and incubated at 30°C for 2 hours before crosslinking with 1% formaldehyde (Sigma) for 15 minutes followed by quenching with 125 mM glycine for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... (25 mg l−1) and sodium salicylate (from 0.1 mM to 2 mM) All reagents were purchased from Sigma Aldrich, except for X-Gal ...
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bioRxiv - Neuroscience 2020Quote: ... 66 These transgenic mice express the GEVI in cortical layer 2/3 pyramidal neurons under the intersectional control of TetO and destabilised Cre-recombinase.70–72 The destabilised Cre-recombinase was stochastically re-stabilised to induce sparse expression of the voltage indicator via two IP injections of a total of 2 × 10−4 mg kg−1 Trimethoprim (TMP, Sigma) over 2 consecutive days as described in Song et al ...
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bioRxiv - Microbiology 2021Quote: ... in serum-free media supplemented with 0,1 μg/ml L-1-p-Tosylamino-2-phenylethyl chloromethylketone (TPCK)-treated trypsin (Sigma-Aldrich). The supernatant was harvested at 72 h post infection when cytopathic effects were observed (with around 50% cell death) ...
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bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... with PBS +0.05% Tween20 and bound FLAG-ACE-2 was detected with HRP-conjugated anti-FLAG antibody (1:20,000, Sigma cat# A8592) and peroxidase substrate (KPL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were irradiated using 8 J/m2 UV and labelled for 7 hours with 20 µM 5-Ethynyl-2’-deoxyuridin (EdU) and 1 µM Floxouridine (Sigma). Subsequently ...
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bioRxiv - Cancer Biology 2022Quote: ... and CH-5high/CH-6high subpopulation breast cancer cells (cell numbers: 0.5million) were mixed with 100μl Matrigel/cell culture media mixture (Matrigel: cell culture media = 1:2) (Sigma or Corning). Indicated cancer cells/Matrigel/ cell culture media mixtures were injected into NOD/SCID female for breast cancer xenograft (the Jackson Laboratory ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in high-salt TBS + 2% BSA + 0.1% Triton X-100 at 1:4000 (mouse anti-a-tubulin, Sigma #T6199) and 2 µg/mL (rabbit anti-CENP-A ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 µg ml−1 of L-ascorbic acid or 2-Phospho-L-ascorbic acid trisodium salt (Vitamin C) (Sigma). NSCs were cultured in NSC complete medium consisting of DMEM/F-12 Media ...
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bioRxiv - Molecular Biology 2022Quote: ... Dynabeads following the first pulldown were resuspended in Re-ChIP elution buffer (20 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 100 µg of 3X FLAG peptide [Sigma, #F4799]), and incubated in 4 °C for 1 hour with rotation to selectively elute FLAG-fusion protein ...
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bioRxiv - Microbiology 2022Quote: We cultured the cells with either Luria-Bertani (LB) broth (Difco) or M9 minimal medium (Difco) supplemented with 1/2 MEM amino acids solution (SIGMA) and 0.2% (w/v ...
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bioRxiv - Immunology 2022Quote: ... the different mouse groups were killed 1 hour after retro-orbital injection of 100 μl of 2% Evans blue (EB) dye (Sigma) and were perfused intracardially with 15 ml of PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and CH-5high/CH-6high subpopulation breast cancer cells (cell numbers: 0.5million) were mixed with 100μl Matrigel/cell culture media mixture (Matrigel: cell culture media = 1:2) (Sigma or Corning). Indicated cancer cells/Matrigel/ cell culture media mixtures were injected into NOD/SCID female for breast cancer xenograft (the Jackson Laboratory ...
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bioRxiv - Cell Biology 2022Quote: A solution of methimazole (1-methyl-3H-imidazole-2-thione) (CAS 60-56-0; MW, 114.17 g/mol; purity, ≥99%; Sigma-Aldrich) was used to induce hypothyroidism ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed in 2% CHAPS lysis buffer (20 mmol/l Tris-HCl, pH 7.5; 150 mmol/l NaCl; 1 mmol/l EDTA; 2% CHAPS; Sigma-Aldrich) with protease and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were then washed four times with lysis buffer and eluted in 2x Laemmli buffer containing 1% 2-mercaptoethanol (Sigma) by heating at 95°C for 3min.
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bioRxiv - Pathology 2021Quote: Left lungs were put in 2 ml microtubes with 1 ml prewarmed PBS containing 200 units of collagenase (Sigma, C9891) and 0.9 mM of calcium and magnesium ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... washed twice in 1x PBS and resuspended in 2 ml co-IP lysis buffer (50 mM Tris-HCl pH 7.5, 1% Nonidet P 40 Substitute (Sigma #74385), 0.25% deoxycholate Na ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times with PBS and incubated for 2 h with blocking and permeabilization buffer (1% BSA, 5% goat serum, 0.15% saponin (Sigma Aldrich)) ...
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bioRxiv - Cancer Biology 2021Quote: Cells were washed with cold DPBS for two times and then lysed in 2× Laemmli buffer (2% SDS, 20% glycerol, and 125 mM Tris-HCl, pH 6.8) supplemented with 1× protease inhibitor cocktail (Sigma, P8340). The cell lysate was scraped and sonicated ...
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bioRxiv - Biophysics 2020Quote: ... For reduction and alkylation of the proteins, proteins were incubated with SDC buffer (1% Sodiumdeoxycholate, 40nmM 2-Cloroacetamide (Sigma-Aldrich), 10 mM tris(2-carboxyethyl ...
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bioRxiv - Molecular Biology 2020Quote: ... then 1-2 mg (as indicated) of total protein was incubated with pre-washed M2 α-FLAG magnetic beads (Sigma) or MagStrep “type3” XT beads (IBA ...
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bioRxiv - Microbiology 2020Quote: ... in serum-free media supplemented with 0,1 μg/ml L-1-p-Tosylamino-2-phenylethyl chloromethylketone (TPCK)-treated trypsin (Sigma–Aldrich). The supernatant was harvested at 72 h post infection when cytopathic effects were observed (with around 50% cell death) ...
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bioRxiv - Microbiology 2021Quote: ... The matrix consisted of a 9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid (super-DHB, Sigma-Aldrich) at a final concentration of 10 mg/ml dissolved in chloroform ...
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bioRxiv - Microbiology 2021Quote: ... Frozen samples were thawed on ice for 2 min and 1 mL of HPLC grade methanol (Sigma, 34860-4L-R) added to the pellets ...
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bioRxiv - Biochemistry 2020Quote: ... Bound scFv were detected using murine mAb 9E10 which recognizes the C-terminal c-myc tag (1:50 diluted in 2% MPBST) and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (A0168, Sigma) (1:42000 dilution in 2% MPBST) ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Microbiology 2021Quote: ... Colicins B1-341 GFP/mCherry were expressed with a 6xHis tail at the C’ terminus and cloned into the multiple cloning site 2 of a pACYCDuet-1 (Novagen) plasmid where they were expressed under a T7 promotor ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then washed 2 x 1 h in 100% MeOH followed by overnight incubation at RT in DiChloroMethane:MeOH (DCM, Sigma – 270997) 2:1 ...
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bioRxiv - Microbiology 2021Quote: ... All samples were further concentrated to a final volume of 1-2 ml using 100 kDa Amicon-ultra filters (Merck Millipore).
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bioRxiv - Neuroscience 2022Quote: ... Stemgent, 04-00046) and LDN-193189 (100 nM, Stemgent, 04-0074) along with doxycycline hyclate (2 µg.mL-1, Sigma, D9891) and Y27632 (5 mM ...