Labshake search
Citations for Millipore Sigma :
8451 - 8500 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... the transduced cells were selected with medium containing puromycin (0.2 μg/ml) for one week (EMD Millipore, 540411). Successful transduction was confirmed by the doxycycline-induced GFP signal in 16HBEge-GFP-P2A-WT and 16HBEge-GFP-P2A-Δex23 cells (see ‘Microscopy’).
-
bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated overnight at 4°C with one of the following primary antibodies: FLAG (Sigma-Aldrich, F3165), γH2AX (Millipore ...
-
bioRxiv - Immunology 2020Quote: ... Membranes were blocked and probed with one of the following antibodies: a rabbit antibody to COPA (Sigma, HPA028024) or a mouse antibody to GAPDH (Santa Cruz Biotechnology ...
-
bioRxiv - Immunology 2020Quote: ... coated plates were washed one time with 100 μl/well PBS with 0.05% Tween 20 (PBST) (Sigma-Aldrich), blocked with 100 μl/well of 1% human serum albumin (HAS ...
-
bioRxiv - Biophysics 2021Quote: ... HTT-HAP40 eluted in one narrow-based peak and was concentrated with Amicon ultra 100 kDa filters (Millipore). The purity of purified HTT-HAP40 complex was analysed by SDS-PAGE and silver Coomassie-Blue staining ...
-
bioRxiv - Biochemistry 2020Quote: ... resuspended in 50 mM ammonium bicarbonate solution containing one of the following proteases: 0.2 µg trypsin (T6567, Sigma); 0.17 µg GluC (11420399001 ...
-
bioRxiv - Neuroscience 2020Quote: ... in 0.1M Phosphate Buffer (PB) for one and half hours and visualized with diaminobenzidine staining (Sigma Aldrich, D4293). To detect PNNs ...
-
bioRxiv - Immunology 2021Quote: ... mice were primed in one hind footpad with 25mg antigen emulsified in Hunters Titermax Gold adjuvant (Sigma-Aldrich). At day 10 ...
-
bioRxiv - Cell Biology 2020Quote: ... Sections were then blocked for one hour in blocking solution (DPBS containing 10% goat serum (Sigma Aldrich, G9023), and 0.3% Triton X-100) ...
-
bioRxiv - Molecular Biology 2022Quote: Mice were treated with one dose of BrdU (Sigma #B5002, 10 mg/mL in PBS, 5 mg/kg) by intraperitoneal (i.p. ...
-
bioRxiv - Genomics 2022Quote: ... membranes were incubated for 5 min with one volume (generally 310 μl) of phenol:chloroform (Sigma, Cat. No. P3803). The membrane backers were removed from the tube with tweezers leaving just the mostly dissolved EVA membranes and tissue behind ...
-
bioRxiv - Cell Biology 2022Quote: ... The sgRNAs were then cloned into the All-in-One Cas9D10A nickase vector using DNA oligos (Sigma-Aldrich) using the BsaI and BbsI recognition sites ...
-
bioRxiv - Neuroscience 2022Quote: ... administered in one eye and the contralateral eye receiving 0.0001% DMSO (diluted in 0.9% sodium chloride solution [Sigma]). GSK-J4 contained 0.0001% DMSO and was further diluted in 0.9% sodium chloride solution to make a 100µM solution ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were incubated with secondary antibodies for one hour at room temperature (RT) and mounted using Mowiol (Sigma); Hoechst (Invitrogen ...
-
bioRxiv - Microbiology 2021Quote: ... One hundred and twenty-five microliters were then transferred to a Multiscreen Solvinert Filter Plate 0.45 μM (Millipore) placed on top of a 96-well 0.5 mL polypropylene microplate (Agilent ...
-
bioRxiv - Molecular Biology 2020Quote: ... with one well being coated with anti-FLAG antibody (10 μg/ml in 1x PBS, F1804 Sigma-Aldrich), and one control well without antibodies ...
-
bioRxiv - Neuroscience 2020Quote: Following a one-hour blocking step in Tris-buffered saline Tween-20 (TBST, Sigma Aldrich, St. Louis, MO) containing 5% nonfat dry milk ...
-
bioRxiv - Genetics 2020Quote: ... and stained for one hour with anti-NeuN conjugated to biotin (cat no. MAB377B, EMD Millipore, Burlington MA), and for one hour with Brilliant Violet 711 Streptavadin (cat no ...
-
bioRxiv - Cell Biology 2022Quote: ... and released in fresh medium containing either one of the following drugs: 10 μM MG132 (proteasome inhibitor, Sigma), 50 nM okadaic acid (PP1 and PP2A inhibitor ...
-
bioRxiv - Developmental Biology 2022Quote: ... For fibronectin deletion (FN-/-) one metanephric kidney was treated with (Z)-4-hydroxytamoxifen (HT; 500 nM, Sigma-Aldrich) whereas the contralateral kidney was treated with control medium only and served as control (FN+/+) ...
-
bioRxiv - Neuroscience 2022Quote: Digoxygenin (DIG)-labeled probes were produced with the SuperScriptTM III One-Step RT-PCR kit (Sigma 12574-026) using specific primers for the Bgl-FaNaC transcript (Forward ...
-
bioRxiv - Molecular Biology 2022Quote: ... brain from one mouse from each group were removed under anaesthetized condition (by ketamine-xylazine cocktail, Sigma-Aldrich). Mice brains were placed in dimethyl formamide (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2022Quote: ... for one hour and 20 minutes and then revealed with crystal violet 0.04% (Sigma-Aldrich, catalog #6158, USA) for plaque visualization and counting.
-
bioRxiv - Physiology 2024Quote: ... One half was incubated for 30 min at 37 °C in 100 mM paraquat (Sigma-Aldrich, Taufkirchen, Germany) prepared in HEPES-buffered Ringer solution ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Vacutainers without additives (Vacuette Blood collection tube, 3.0ml, no additive, Greiner Bio-One, Sigma Aldrich, St Louis, MO) were used to collect the probe samples and an additional 10ml of air was removed to increase negative pressure in each vacutainer to increase sampling speed ...
-
bioRxiv - Neuroscience 2023Quote: ... for one minute then blocked for two hours at room temperature in DPBS containing 5 mM EDTA (Sigma), 1% fish gelatin (Sigma) ...
-
bioRxiv - Immunology 2023Quote: ... pericardial and mediastinal adipose tissues were fixed for one hour on ice in 10% NBF (Fisher or Sigma) then permeabilized in PBS 1% Triton-X 100 (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... pH 7.2) in the presence of cocktail protease inhibitor (one tablet of cOmplete per 50 mL [Sigma-Aldrich]). Three replicates of 150µL from this resuspension were pipetted to glass vials for each sample ...
-
bioRxiv - Genomics 2023Quote: ... one vial of a frozen hiPSC line was thawed into mTeSR1 medium containing 10 μM ROCK Inhibitor (Sigma), plated on one well of a six-well plate coated with Matrigel ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Each well had one fish placed in 450 μl of fish water without or with PTZ (Sigma-Aldrich). 60 zebrafish larvae were used in total ...
-
bioRxiv - Developmental Biology 2024Quote: ... up to three S9-S9+ limbs were cultured over one 0.45 µm pore Durapore membrane filter (SLGVR33RS, Millipore) floating on DMEM medium (12100046 ...
-
bioRxiv - Cell Biology 2024Quote: Tissue samples were harvested and fixed for one hour at 4 °C in 2.5% glutaraldehyde (Sigma-Aldrich – G5882) in sodium cacodylate buffer 0.1M pH 7.4 (Sigma-Aldrich - C0250 ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by permeabilization for 15 minutes (0.1% triton X-100 + 0.1M Glycine in PBS) and blocking for one hour (5% donkey serum (DS) (Sigma) + 2% bovine serum albumin (BSA ...
-
bioRxiv - Developmental Biology 2024Quote: ... One-cell-stage zygotes were collected from the E0.5 oviduct ampulla and treated with hyaluronidase (Sigma-Aldrich, H4272) to remove cumulus cells ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
-
bioRxiv - Immunology 2020Quote: ... injection of 106 hemocytes from late third instar larvae of the lethal(3)malignant blood neoplasm [l(3)mbn1] mutant larvae in Drosophila Ringer’s solution (Sigma-Aldrich, St. Louis, MI, USA). Booster injections were given 4 ...
-
bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips were then coated with a solution of 3% (v/v) (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich, St. Louis, MO) in ethanol for 20 min at room temperature ...
-
bioRxiv - Biophysics 2023Quote: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) dry powders were purchased from Sigma Aldrich (St. Louis, MO, USA). Different amounts of DOPC and DOTAP were used to tune the surface charge of the employed liposomes ...
-
bioRxiv - Cell Biology 2023Quote: ... strains containing a degron tag were arrested at 30 °C for 3 hours (total) and then treated with 250 μM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) for 30 minutes before harvesting ...
-
bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
-
bioRxiv - Biophysics 2023Quote: ... the quench buffer also included 3 μL of 0.8% GDN and 3 μL of 300 mg/ml aqueous suspension of ZrO2-coated silica (Sigma-Aldrich, reference no. 55261-U). The resulting mixture was incubated on ice for 1 min to remove lipids and solubilize prestin in GDN before being filtered through a cellulose acetate spin cup (Thermo Pierce ...
-
bioRxiv - Genomics 2024Quote: ... ratio by a Gibson approach with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
-
bioRxiv - Immunology 2024Quote: ... 500 µL of viral supernatant was added to 3×106 cells in a final volume of 3 mL growth media supplemented with 8 µg/mL polybrene (Millipore Sigma TR-1003-G) in a 12-well dish ...
-
bioRxiv - Genomics 2024Quote: ... and ligated by Gibson with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
-
bioRxiv - Physiology 2020Quote: ... inhibitor 2-bromohexadecanoic acid (2-bromo-palmitate; 2-Br) [IC50 of ∼4 µM] (Varner et al., 2003) were purchased from Sigma-Aldrich (St. Louis, MO). Bradford protein assay kit as well as anti-mouse and anti-rabbit secondary antibodies were obtained from Bio-Rad (Hercules ...
-
bioRxiv - Cell Biology 2020Quote: ... The fixed cells were then incubated with antibodies diluted in 1% BSA and 0.1% saponin at ambient temperature: 2 h with primary antibodies (anti-HDDC3, 1:25, cat# HPA040895, Sigma-Aldrich Co. ...
-
bioRxiv - Cell Biology 2020Quote: ... 10 μM carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) and 2 µM Antimicyn A / 1 µM Rotenone (all Sigma-Aldrich; Missouri, USA). The data were analyzed using Wave 2.6 Software ...
-
bioRxiv - Developmental Biology 2021Quote: Dissected brain-ring gland complexes were initially fixed in PBS with 2%PFA (cat#16005, Aldrich) and 1% glutaraldehyde (cat#G5882, Sigma) for 2 hours at room temperature followed by overnight incubation at 4°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... The female mice were sacrificed by cervical dislocation and the whole female reproductive tract with the inner contents was collected with the copulation plug at 1 hour or 2 hours after coitus and placed into 4% paraformaldehyde (PFA, Sigma) at 4°C overnight.
-
bioRxiv - Developmental Biology 2021Quote: Fish with mutations and transgenic background at 3.2 or 4 dpf were fixed 1 to 2 days at 4℃ with 4% paraformaldehyde (Sigma, P6148) in PBS ...