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Citations for Millipore Sigma :
801 - 850 of 7201 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... αSyn oligomers were produced by incubating recombinant αSyn to a final concentration of 20 µM with 300 µM DOPAL or dopamine (Sigma-Aldrich) in PBS ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Cell Biology 2021Quote: ... 5a), recombinant mEGFP(−7e) or scGFP(+7e) were dissolved in injection buffer (50 mM K-HEPES, pH 7.4 (Sigma-Aldrich, H3375), 25% glycerol (Applichem ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded on 6-well cell culture plate wells at a density of ∼2 x 105 cells/well with 2 mL of the culture medium containing 103 units/mL ESGRO Recombinant Mouse Leukemia inhibitory factor (LIF) (Millipore #ESG1107) and 2i (1.0 µM PD0325901 Toris #4423 and 3.0 µM CHIR99021 Wako #038-23101) ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... stable PDA cell lines were generated by infecting 70% confluent cultures with the recombinant lentiviral vectors with 10 mg/mL polybrene (TR-1003, Sigma-Aldrich) twice over 2 consecutive days ...
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bioRxiv - Cell Biology 2023Quote: In vitro kinase assays were performed in the presence or absence of different recombinant human atypical PKC (50 or 100 ng of PKC ζ, #14-525 Merk Millipore; 1µg of PKC I/αPar6 complex ...
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bioRxiv - Biochemistry 2024Quote: ... were mixed with 60 ng of recombinant HA-BACH1 in 21 μl of a reaction buffer containing 10 μg of bovine ubiquitin (Sigma-Aldrich), 2 mM ATP ...
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bioRxiv - Neuroscience 2024Quote: ... lateral and medial) were each injected with 10 μl of either PBS or recombinant mouse GDNF (10 μg/ml, Sigma-Aldrich) using 31-gauge insulin syringes without damaging any innervating nerves ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM carnitine) with or without supplementation of 100 ng/ml (13.16 nmol/l) IGF1 (human recombinant IGF1, I3769, Sigma-Aldrich, Germany). Medium was changed three times per week and 48 hours before harvest ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore, Darmstadt ...
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bioRxiv - Neuroscience 2021Quote: ... The protein concentration was then determined using the Bradford protein concentration assay (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Genomics 2020Quote: ... of GAPDH (cytoplasmic protein) and Histone H3 (nuclear protein) using rabbit anti-GAPDH antibody (1:5000, Sigma) and rabbit anti-histone H3 antibody (1:10000 ...
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bioRxiv - Microbiology 2019Quote: Quantified protein (between 80-160 μg) was incubated with protein-specific antibodies (either R anti-HA (Sigma) or anti-NS4A ...
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bioRxiv - Biochemistry 2021Quote: Protein concentrations were measured with the Bio-Rad Protein Assay Kit using bovine serum albumin (Sigma-Aldrich) as reference.
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bioRxiv - Cell Biology 2021Quote: The protein-protein interactions in fixed HeLa cells were assessed using in situ PLA (Duolink®SIGMA) according with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... BCA was performed on the freshly isolated proteins using a Pierce BCA protein assay kit (Sigma, 23227) to determine the protein concentration ...
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bioRxiv - Cell Biology 2022Quote: The protein-protein interactions in fixed HeLa cells were assessed using in situ PLA (Duolink®SIGMA) according with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: PTP-tagged protein expression was demonstrated by immunoblotting with a rabbit polyclonal anti-protein A antibody (SIGMA) as described previously [13] ...
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bioRxiv - Systems Biology 2022Quote: ... The dried pellet was used for protein analysis using Bicinchoninic Acid Kit for Protein Determination (Sigma Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... The purified protein was separated by SDS-PAGE and protein was excised after InstantBlue™ (Sigma-Aldrich) staining ...
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bioRxiv - Immunology 2019Quote: ... Protein concentration was determined using BCA protein assay reagent (Pierce Chemical Co.) with bovine serum albumin (Sigma) as the standard.
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bioRxiv - Cancer Biology 2020Quote: ... cell lysate containing about 500 μg protein was cleared with 30 μl Protein A (Millipore, Billerica, MA) for 4 h ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... 50mM HEPES pH6.6-8.5 depending on protein isoelectric point) containing complete EDTA-free protein inhibitors (Sigma-Aldrich), centrifuged for 10 min at 1,620 rcf at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Protein estimation was done on the depleted plasma protein samples using the Bradford assay (Sigma-Aldrich, USA). In the SWATH-MS analysis following steps like reduction ...
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bioRxiv - Biochemistry 2021Quote: Protein-protein interactions were analyzed using Duolink in situ orange starter fluorescence kit (mouse/rabbit, Sigma-Aldrich) in human osteosarcoma U2OS cells (WT ...
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bioRxiv - Microbiology 2020Quote: ... total protein concentration was measured in triplicates by Total Protein kit (Sigma-Aldrich, St. Luis, MO, US) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: Mice were immunized intradermally (i.d.) in the ear with 10μg OVA protein or KLH protein (Sigma Aldritch) emulsified in complete Freund’s adjuvant (CFA ...
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bioRxiv - Cell Biology 2022Quote: ... or Protein G Sepharose beads (Protein G Sepharose® 4 Fast Flow, Sigma-Aldrich, GE17-0618-01) used for anti-Ollas antibody IPs ...
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bioRxiv - Immunology 2022Quote: ... with protein concentration determined by Coomassie G-250 Bradford protein assay kit following manufacturer’s instructions (Sigma, UK).
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bioRxiv - Plant Biology 2022Quote: Protein phosphorylation was performed by incubating 1 µg of PpeAld6PRase or myelin binding protein (MBP; Sigma-Aldrich) with the corresponding protein kinase in a standard medium containing 20 mM Tris-HCl pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: FLC-Venus protein stability was assayed with the de novo protein synthesis inhibitor cycloheximide (C1988, Sigma-Aldrich) following the procedures in (Zhao ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was retrieved and protein concentration was measured by BCA protein assay (Novagen, (Smith et al. 1985). Protein concentrations were adjusted in all the samples to 800 µg/ml ...
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bioRxiv - Biochemistry 2022Quote: Protein—protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma, DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: Proteins were extracted from the conditioned medium using the protein precipitation kit (#2100, Millipore Sigma, Burlington, MA) following the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2022Quote: Protein-protein interactions were studied using a Duolink In Situ Orange Starter Kit Mouse/Rabbit (Sigma DUO92102) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was collected and total protein content determined with a BCA Protein Assay (Sigma, Poole, UK). A total of 20μg protein extract for each sample was separated by electrophoresis on Nu-PAGE™ 4-12% ...
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bioRxiv - Plant Biology 2020Quote: ... The protein amount of the resuspended membrane fractions was quantified by Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) and equilibrated between different samples ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein was measured using the protein extract for PEPCK activity using Bradford reagent (Millipore Sigma; Cat: B6916) and commercial bovine serum albumin standards (Thermo Fisher 23208) ...
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bioRxiv - Developmental Biology 2021Quote: ... Total proteins were extracted from cells with the M-PER Mammalian Protein Extraction Reagent (Sigma, GE28-9412-79) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich, St. Louis, Missouri United States) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... solium larval extracts were assessed for protein concentration using a BCA or Bradford protein assay kit (Sigma-Aldrich), respectively.
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk ...
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bioRxiv - Microbiology 2020Quote: Oxidized proteins containing carbonyl groups were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma-Aldrich: cat#S7150). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... Total protein was measured using the protein extract for PEPCK activity using Bradford reagent (Millipore Sigma; Cat: B6916) and a standard curve using commercial bovine serum albumin standards (Thermo Fisher ...