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Citations for Millipore Sigma :
751 - 800 of 7201 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Biophysics 2020Quote: ... previously coated with Protein A (Sigma), blocked in TBS-BSA buffer and subsequently incubated with anti-myc antibody (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... BCA protein Assay Kit (Sigma-Aldrich) was used to quantify proteins in all the fractions and 30 µg of proteins per fraction were separated by SDS-PAGE for immunoblot.
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bioRxiv - Biochemistry 2021Quote: ... bound to Protein-A Sepharose (Sigma), and ubiquitination reactions were performed as described8 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein A-Sepharose beads (Sigma-Aldrich) were used to purify the antibody/protein complex ...
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bioRxiv - Immunology 2022Quote: Magna Beads Protein A+G (Millipore) were blocked with PBS + BSA (5mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... protein-A agarose Fastflow beads (Millipore) were washed twice with IP Buffer and then blocked in IP Buffer containing 2% BSA by rotating overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Magnetic anti-Protein A beads (Sigma) were washed in a Beads Wash Buffer (BWB ...
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bioRxiv - Developmental Biology 2022Quote: ... Sepharose coupled with protein G (Sigma) was blocked with 5% BSA (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... protein A/G agarose beads (Millipore) were added to the chromatin-antibody complex and incubated for one hour at 4°C on a nutator ...
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bioRxiv - Molecular Biology 2019Quote: ... Dynabeads protein-A (or -G, Millipore) were added and incubated for 4 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... protein G agrarose (60 μl, Millipore) was incubated with anti-phosphotyrosine antibodies (12 μg 4G10 (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... catalase (40,000 units/mg protein, SIGMA) was added to the reaction mixture to deplete the resulting hydrogen peroxide ...
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bioRxiv - Cell Biology 2020Quote: ... nonstructural protein 9 (NSP9, Sigma, SAB3701435), FLAG (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... protein A-HRP (Millipore, Billerica, MA), goat anti-mouse-HRP (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were visualized with ECL (Millipore) using a Pxie machine.
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bioRxiv - Genetics 2020Quote: ... Proteins were digested with trypsin (Sigma) overnight at 37°C ...
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bioRxiv - Genetics 2020Quote: ... and 10% protein (BSA (Sigma A1470), HSA (Sigma A8763) ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by protein G beads (Sigma).
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bioRxiv - Physiology 2023Quote: ... protein concentration was quantified by Novagen BSA assay (71285-3 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were reduced with DTT (Sigma) and alkylated with iodoacetamide (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were digested with trypsin (Sigma) overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... and Apo-1 protein sequence (Sigma) along with Proteome Discoverer™ contaminant database ...
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bioRxiv - Neuroscience 2023Quote: ... 2.0 Protein Detection System (Millipore/Sigma) for 10 min and incubated with monoclonal anti-C-cadherin antibody ...
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bioRxiv - Neuroscience 2023Quote: ... 2.0 Protein Detection System (Millipore/Sigma) for 10 min and incubated with monoclonal anti-C-cadherin antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A Sepharose® beads (Sigma) were coated with PBS + 0.3% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... protein inhibitor cocktail (539134, Millipore Sigma), sodium fluoride ...
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bioRxiv - Neuroscience 2023Quote: ... protein A Sepharose (Sigma-Aldrich #P3391) was added to the mixture and incubated for 4 hr at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and Protein A Sepharose (Sigma-Aldrich), and purified mAbs were buffer-exchanged into phosphate buffered saline (PBS).
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated hyaluronic acid binding protein (Millipore) was used at 10 μg/mL ...
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bioRxiv - Microbiology 2024Quote: ... Horseradish peroxidase conjugated Protein G (Sigma) was used as 2ndaly antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... or protein A-agarose beads (Sigma) for 2-3 hours at 4℃ ...
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bioRxiv - Microbiology 2024Quote: ... Protein A- agarose beads (Millipore Sigma) were washed 3 times with 1 × immunoprecipitation (IP ...
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bioRxiv - Developmental Biology 2024Quote: ... prewashed Protein G agarose beads (Millipore) were added to the protein antibody mix and incubated at 4°C for 4 hours on an orbital shaker ...
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bioRxiv - Cancer Biology 2022Quote: ... protein were combined with a mixture of 50 % (v/v) protein A and 50 % (v/v) protein G beads (Sigma-Aldrich Inc., Cat#P9424 and Cat#P3296) and 0.5-1 μg antibody in lysis buffer overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells and large quantities of His6 tagged recombinant Nbs were purified with 1ml of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) following periplasmic expression through osmotic shock ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was cut in two (one half for the commercial and one half for the recombinant anti-FLAG™-M2) and each half was probed with either commercial (Sigma) or recombinant anti-FLAG™-M2 at 1 µg/mL in PBS for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant LymCBP HAT domain (200 ng) were incubated with the histone H1 purified from calf thymus (Sigma-Aldrich, 1 μg) and 7.4 kBq of [14C]-acetyl-CoA (Moravek ...
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bioRxiv - Microbiology 2021Quote: ... Sublines expressing a doxycycline-inducible BZLF1 transactivator were produced by culturing 106 LCL cells with the recombinant lentivirus in presence of 8 μg/ml polybrene (TR-1003-G, Sigma-Aldrich) for 24 hours followed by replacement of the infection medium with fresh complete medium ...
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant mTLR9-ECD was titrated into a solution of oligonucleotide labeled at the 5’ end with Alexa 488 Fluor (Sigma-Aldrich). The oligonucleotide concentration ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...