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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The in vitro phosphorylation is conducted using the human Src-GST protein (Sigma-Aldrich, Catalogue number: S1076) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Biophysics 2024Quote: ... containing (g/100mL): 3 g 13C6-glucose (Sigma, GmbH); 2 g casein enzyme hydrolysate ...
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bioRxiv - Bioengineering 2022Quote: ... the mononuclear cell fraction of rat bone marrow or human peripheral blood was obtained by layering the cell suspension over Histopaque®-1083 (density: 1.083 g/mL, Sigma, ON, Canada) or Ficoll (density ...
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bioRxiv - Cell Biology 2021Quote: ... phalloidin coupled with TRITC (#P1951, Sigma Aldrich) was added at this step ...
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bioRxiv - Cell Biology 2020Quote: ... and horseradish peroxidase-coupled secondary antibody (Millipore). Enhanced chemiluminescence detection (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... were coupled with anti-FLAG antibody (Sigma) and used for the immunoprecipitation of FLAG-ALBH8 complexes ...
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bioRxiv - Cell Biology 2021Quote: ... anti-HA antibody-coupled agarose beads (Sigma) were used ...
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bioRxiv - Cancer Biology 2019Quote: ... coupled to anti-Flag M2 (Millipore Sigma) followed by 3xFlag elution.
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bioRxiv - Plant Biology 2021Quote: ... or alkaline phosphatase-coupled (Sigma-Aldrich, www.sigmaaldrich.com) secondary antibodies were used ...
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bioRxiv - Physiology 2023Quote: ... followed by fluorochrome-coupled secondary antibodies (Millipore) and DAPI counterstaining (Abcam) ...
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bioRxiv - Biochemistry 2023Quote: ... coupled to rabbit IgG (Sigma-Aldrich, S1265) as described previously (Xia et al ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary peroxidase-coupled antibodies (Sigma, France) or Alexa fluor 647 nm (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... HRP-coupled anti-mouse secondary antibodies (Sigma) were used.
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bioRxiv - Biochemistry 2023Quote: ... coupled to anti-FLAG antibody (Sigma F7425) and incubated overnight at 4 °C on a wheel ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein-protein interactions were assessed using the PLA Duolink in situ starter kit (Sigma Aldrich, DUO92101) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using the Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich Cat# BCA1) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Protein-protein interactions were detected using the Detection Kit Red (DUO92008) according to the manufacturer’s (Sigma) instructions ...
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bioRxiv - Physiology 2023Quote: ... The total protein content in samples was obtained using a BCA protein assay kit (Sigma-Aldrich) with the SpectraMax Paradigm microplate reader (Molecular Devices ...
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bioRxiv - Biochemistry 2022Quote: ... AviTagged trimeric S and RBD proteins were biotinylated using the Enzymatic Protein Biotinylation Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2019Quote: Samples were assayed for insulin or proinsulin content using ELISA according to the instructions of the manufacturers (Rat/Mouse insulin ELISA, Millipore, #EZRMI-13K ...
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bioRxiv - Cell Biology 2019Quote: Samples were assayed for insulin or proinsulin content using ELISA according to the instructions of the manufacturers (Rat/Mouse insulin ELISA, Millipore, #EZRMI-13K ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The lysate was centrifuged to remove cell debris and then incubated with Protein G Sepharose Fast Flow (Sigma-Aldrich) for 1 h at 4°C to remove nonspecifically bound proteins ...
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bioRxiv - Genomics 2021Quote: ... Antibodies-chromatin complexes were then pulled-down using 20 μL Magna ChIP™ protein A+G magnetic beads (Millipore) by incubating with rotation (20 rpm ...
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bioRxiv - Immunology 2019Quote: ... chromatin was precleared by rotating for 2 h at 4°C with 100 μl of 50% protein A/G slurry (0.2 mg/ml sheared salmon sperm DNA, 0.5 mg/ml BSA, and 50% protein A/G; Sigma-Aldrich), 0.3−0.5×106 cell equivalents were saved as input ...
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bioRxiv - Neuroscience 2019Quote: ... The mixture was then incubated with 20 μL of 50% protein G plus-agarose (Sigma-Aldrich, St. Louis, MO) for 4 h at 4°C with gentle rocking ...
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bioRxiv - Cancer Biology 2019Quote: ... ChIP dilution buffer was used to dilute the sheared chromatin followed by preclearing with Protein G Agarose beads (Sigma) for 30min ...
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bioRxiv - Biophysics 2021Quote: ... were used at 1,000 × g and 4 °C to exchange purified protein samples to 250 mM ammonium acetate (99.99 % purity; Sigma-Aldrich), pH 8.0 as buffer surrogate ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Neuroscience 2020Quote: ... The rest was incubated with 30μl of Protein G Dynabeads and 3 μl of anti-FLAG M2 antibody (Sigma) overnight to immunoprecipitate (IP ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... were used at 14,000 x g and 4 °C to exchange purified protein samples to 150 mM ammonium acetate (99.99 %; Sigma-Aldrich), pH 7.2 ...
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bioRxiv - Cancer Biology 2019Quote: ... a sample mixture of 10 μg protein and non-reducing sample buffer was loaded on a polyacrylamide gel containing gelatin (0.25 mg/ml; Sigma). The gel was then soaked (36 h ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was allowed to proceed by rotation at 4°C for 2hrs and then immunoprecipitated with 60μl Protein G Sepharose (Sigma). The sediments were washed twice with HEPES/KCl buffer + 0.25% Triton-X100 ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were immunoprecipitated with ACC antibody (CST) or ACC2 antibody (CST) and EZview Red Protein G Affinity Gel (Sigma).
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bioRxiv - Immunology 2020Quote: ... one 10-cm dish of packaging cell line at roughly 70% density was transfected with the target construct and two supplementing vectors providing the VSV gag/pol and VSV-G-env proteins (6 μg of DNA each) using polyethylenimine (22.5 μg/ml, Sigma) and Polybrene (4 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: The lysates of HEK-293T cells transfected with circPTPN12 plasmid were incubated with Protein-A/G agarose beads (Millipore) and antibody against Ago2 (Abcam ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 16,000 g for 10 min and protein concentration was normalized by Amidoblack staining (Sigma). Western blotting was performed following standard protocols as described below ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then spun at 21,000 g for 15 min at 4°C and protein concentration determined using Bradford assay (Sigma). Approximately 3-4mg of total cell extract was mixed with 20μl of M2-anti-FLAG agarose beads (bed volume ...
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bioRxiv - Microbiology 2023Quote: ... 4µg of the appropriate antibody and 20µl of Magnetic protein A/G beads (Magnachip, EMD-Millipore, Billerica, MA, USA) were used for the IP step ...
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bioRxiv - Genetics 2024Quote: ... 1mg of cell lysate was incubated with blocked Protein G beads and monoclonal L1 ORF1p antibody (EMD Millipore, MABC1152) at 12 rpm at 4°C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... alkylation and tryptic digestion (50 µg of protein per sample) was performed in centrifugal filters (Amicon Ultra-0.5, 30 kDa cut-off, Merck Millipore) according to the FASP protocol (Wiśniewski et al. ...
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bioRxiv - Immunology 2022Quote: ... LPS and insulin and ELISA against cardiolipin (Sigma) were performed as previously described48 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the ELISA substrate 3,3’,5,5’- tetramethylbenzidine (Sigma Aldrich) was used to visualise the reaction ...
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bioRxiv - Immunology 2020Quote: ... All ELISA plates were developed with TMB (Sigma) and stopped with 1N sulfuric acid ...
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bioRxiv - Immunology 2020Quote: ... ELISAs were developed with 3,3’,5,5’-Tetramethylbenzidine (Sigma) and optical densities were acquired with a Multiskan™FC (Thermo Scientific).
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bioRxiv - Biophysics 2020Quote: ... ELISA signals were developed by adding TMB (Sigma) in 50 mM Na2HPO4 ...
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bioRxiv - Genomics 2023Quote: ... Adiponectin concentrations were determined by using ELISA (Millipore). Plasma alanine transaminase (ALT ...