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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The pre-cleared supernatants were incubated with protein A- or G-Sepharose 4B beads (Sigma Aldrich or Amersham) and with primary antibodies for 2–4 h at 4 °C ...
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bioRxiv - Genetics 2021Quote: Three-fold diluted nuclear extracts were pre-cleared with 50 μL Protein-G agarose beads (Millipore, 16-266) for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 hours and subsequently incubated with Dynabeads Protein G and an anti-Flag antibody (Sigma-Aldrich, F1804) at 4°C with rotation for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... and then purified from this culture media using Protein G Sepharose beads (Cat# GE17-0618-01, Millipore Sigma), as described previously (Naito et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were then analysed by Coomassie staining using Brilliant Blue G colloidal concentrate (Sigma Aldrich, B2025) on 4–12% NuPAGE Bis-Tris gels (Life Technologies).
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bioRxiv - Physiology 2022Quote: ... by adding 20 μL of magnetic beads (Magna ChIP Protein G Magnetic Beads C #16-662, Sigma-Aldrich) and a combination of two anti BMAL1 antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The remaining RNA was combined with 25 μl of Magna ChIP Protein A+G magnetic beads (Merck Millipore) coated in 5μl of anti-m6A antibody (Merck Millipore ...
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bioRxiv - Biochemistry 2023Quote: mAb TnI-1 (IgG1) was purified from mouse hybridoma ascites fluid using Protein G chromatography (Millipore Sigma P7700). TnI-1 IgG eluted with 50 mM glycine-HCl ...
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bioRxiv - Genomics 2023Quote: ... (b) Reduce background signal by pre-clearing with 20µL of Protein A/G magnetic beads (Millipore 16-663) per 1mL of sample for 2 hours at 4°C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... or with 1-5 μg of the appropriate antibody and then with Protein G Sepharose beads (Sigma, P3296). The following day ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X PPI] and precleared by 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated for 2 hrs at 4 °C with Protein A/G Mix Magnetic Beads (Cat# LSKMAGAG10, Millipore). After washing with dilution buffer ...
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bioRxiv - Physiology 2022Quote: ... coupled to a MINUS probe using the Duolink In Situ Probemaker MINUS kit (catalog no. DUO92010, Sigma-Aldrich). Images were captured with a LSM 780 confocal microscope (ZEISS).
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bioRxiv - Molecular Biology 2019Quote: ... all 42 deep well plate cultures were combined and subject to a midiprep (Sigma, NA0200).
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bioRxiv - Developmental Biology 2020Quote: R1 mESC [42] were cultured on 0.3% type B gelatin (Sigma-Aldrich, catalog no. G9382) coated 60 mm cell culture plates in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Molecular Biology 2020Quote: ... The third population was grown with media containing 2.5uM Etoposide (Sigma-Aldrich; 33419-42-0) (senescence sample) ...
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bioRxiv - Synthetic Biology 2023Quote: 90 mL (32°C experiments) or 100 mL (42°C experiments) of LB medium (Sigma) with 75 μg/mL S-gal ...
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bioRxiv - Molecular Biology 2019Quote: EZ-Magna RNA-binding protein immunoprecipitation kit (Millipore, USA) was performed according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using enhanced chemiluminescent detection kit (Millipore). To detect changes in the protein glycosylation ...
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bioRxiv - Cell Biology 2023Quote: ... immunoreactive proteins were detected using Immobilon Western Kit (Millipore). Protein bands from each blot were observed by an imaging system (ChemiDoc XRS+ ...
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bioRxiv - Microbiology 2023Quote: ... Protein was estimated using BCA kit (Sigma Aldrich, USA) and 20 µg protein was loaded on 12% SDS-PAGE gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Elutions were precipitated using ProteoExtract Protein precipitation kit (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using the BCA kit (Sigma), except for insoluble fractions due to due to formic acid’s denaturation effect ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were embedded in Durcupan resin (20 g component A, 20 g component B, 0.6 g component C, 0.4 g component D; Sigma Canada, Toronto, cat# 44610) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... and GM-CSF levels were quantified using ELISA kits pre-coated with indicated capture antibodies per manufacturer’s instructions (Sigma). IL-6 levels were preliminarily detected using a Q-Plex Human cytokine screen (16-plex ...
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bioRxiv - Cell Biology 2021Quote: Plasma leptin levels were determined using a commercially available mouse leptin ELISA kit (EMD Millipore, St. Charles, MO, USA). The intra-assay variation of the kit was between 1.06-1.76% ...
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bioRxiv - Neuroscience 2022Quote: ... we used 100 μM picrotoxin (an antagonist of GABAA and GABAC receptors; Sigma). To block glycine receptors ...
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bioRxiv - Neuroscience 2022Quote: ... we used 5 μM SR-95531 (gabazine, an antagonist of GABAA receptors; Sigma). To block both GABAA and GABAC receptors ...
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bioRxiv - Neuroscience 2020Quote: ... The GABAA receptor agonist muscimol (1 mg/mL, Sigma-Aldrich, St. Louis, MO) or vehicle (0.9% sterile saline ...
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bioRxiv - Neuroscience 2021Quote: ... and the N-methyl-D-aspartate receptor agonist D-Glutamate (0.75mM, Sigma-Aldrich) was bath applied to the spinal cord.
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bioRxiv - Neuroscience 2020Quote: ... and (3) GluA2 (mouse anti-glutamate receptor 2; Millipore; used at 1:2000). Cochlear pieces were incubated in species appropriate secondary antibodies coupled to Alexa Fluors in the red ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular solution containing the opioid receptor antagonist naloxone (300 µM, Sigma Aldrich, Germany) was perfused to the MOB slice for at least 10 minutes.
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bioRxiv - Neuroscience 2020Quote: ... the D2 DA receptor (D2R) agonist quinpirole (20 μM, Sigma, St. Louis, MO) was applied at the end of each experiment as a pharmacological test of DA neuron ientity.25 Whole-cell somatodendritic nAChR-mediated currents were evoked in the presence of tetrodotoxin (TTX ...
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bioRxiv - Neuroscience 2020Quote: ... mouse (IgG2a) anti-GluA2 (Glutamate receptor subunit A2) at 1:2000 (#MAB397, Millipore) for labeling post-synaptic receptor patches ...
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bioRxiv - Neuroscience 2020Quote: ... and the β-adrenergic receptor antagonist DL-propranolol (5 mg/kg; Sigma-Aldrich), immediately prior to sleep latency testing ...
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bioRxiv - Neuroscience 2019Quote: ... and GABA receptors was measured by application of 100 μM glutamic acid (Sigma) or 3 μM GABA (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... and glutamate receptors (NBQX, 10 μM, Tocris; and DL-AP5, 30 μM, Sigma).
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bioRxiv - Cell Biology 2022Quote: ... a mouse anti-GABAA receptor β2/β3 subunit antibody (Millipore, catalog #: 05-474), or a mouse anti-His antibody.
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bioRxiv - Neuroscience 2022Quote: ... the non-competitive NMDA receptor antagonist MK801 (Sigma-Aldrich, St. Louis, MO, USA) was administered in a separate cohort (0.3 mg/kg ...
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bioRxiv - Neuroscience 2022Quote: ... we used a glutamatergic receptor antagonist (10 μM cyanquixaline, or CNQX, Millipore-Sigma) and a GABAA chloride channel blocker (10 mM picrotoxin ...
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bioRxiv - Neuroscience 2022Quote: ... we used a glutamatergic receptor antagonist (10 μM cyanquixaline, or CNQX, Millipore-Sigma) and a GABAA chloride channel blocker (10 mM picrotoxin ...
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bioRxiv - Physiology 2022Quote: Drugs used throughout studies include MSX-3 (A2A receptor antagonist; #M3568; Millipore Sigma), ketanserin tartrate (5-HT2A/C receptor antagonist ...
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bioRxiv - Neuroscience 2023Quote: The non-selective muscarinic receptor antagonist scopolamine hydrobromide (SCOP; Sigma Aldrich, Ontario, Canada) was dissolved in 0.9% physiological saline and given ip at 0.3 mg/kg based on past evidence that scopolamine successfully blocks destabilization at this dose (Stiver et al. ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and muscarinic acetylcholine receptor antagonist atropine (5 mg kg−1, Y000878, Sigma-Aldrich) were injected intraperitoneally (i,p.)27 ...
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bioRxiv - Neuroscience 2023Quote: ... GluR2/3: Anti-Glutamate Receptor 2 & 3 antibody (1:500, Millipore, Cat# AB1506). c-Fos ...
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bioRxiv - Neuroscience 2024Quote: ... the GABA-A receptor antagonist gabazine (SR-95531, Sigma Aldrich,St. Louis, MO) was applied bilaterally (0.005mM-0.01mM in PBS (Carl Roth ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... Detection of proteins was performed by using primary rabbit anti-human hepsin antibody (Sigma Aldrich, Steinheim, Germany), pAKT (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.