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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Chromatin immuno-precipitation was carried out using 2 µL of a commercially available anti-RNA polymerase II subunit B1 phospho-CTD Ser-5 antibody (Millipore, clone 3E8, cat. no. 04-1572) for RNAP II ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... individual late-cavitating blastocysts of similar morphology from each experimental group were treated with Tyrode’s solution (Sigma-Aldrich) to remove zona pellucida ...
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bioRxiv - Molecular Biology 2019Quote: ... Free thiol groups were blocked with blocking solution (250 mM HEPES, pH 7.7, 1 mM EDTA, 0.1 mM neocuproine [Sigma, P9599] ...
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bioRxiv - Cancer Biology 2019Quote: ... Mice in one of the groups were injected with 25 mg/kg of body weight of Quercetin (Sigma) on every alternate day for a period of 15 days ...
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bioRxiv - Immunology 2021Quote: ... Groups of C57BL/6J male mice were immunized subcutaneously with 50μg BpOmpW in Sigma Adjuvant System (SAS, Sigma) or SAS adjuvant alone as negative control ...
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bioRxiv - Neuroscience 2021Quote: ... Conditioning was performed on groups of around 40-50 flies with 3-octanol (around 95 % purity; Sigma-Aldrich) and 4-methylcyclohexanol (99 % purity ...
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bioRxiv - Genetics 2022Quote: ... 50 thoraces per group were dissected and single nuclei were prepared using the nuclei EZ isolation kit (Sigma) according to manufacturer instructions ...
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bioRxiv - Pathology 2020Quote: ... Male Sprague-Dawley rats (200–220 g, 30 rats/group) were anesthetized using chloral hydrate (Sigma-Aldrich, USA). The control (sham ...
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bioRxiv - Systems Biology 2019Quote: ... Macrophages were assigned to one of three treatment groups: 1) stimulated with 1 μg/mL LPS (Sigma, L2880) and 20 ng/mL IFNγ (R&D ...
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bioRxiv - Zoology 2020Quote: Individuals in the treatment group were injected subcutaneously with Escherichia coli O111:B4 lipopolysaccharides (LPS, Sigma Alrdich, L2630) at a concentration of 4 mg/kg b.w. ...
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... hydroxyl and carboxyl groups were modified with 80 μL of N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) (Sigma-Aldrich) with 1% trimethyl-chlorosilane (TMCS ...
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bioRxiv - Neuroscience 2020Quote: ... were administered haloperidol (0.5 or 2 mg/kg/day; haloperidol; n=12/group: Sigma-Aldrich, Gillingham, Dorset, UK) or vehicle (β-hydroxypropylcyclodextrin ...
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bioRxiv - Immunology 2022Quote: ... Select groups received a 1.5% OVA solution in drinking water replaced every 48 h (grade V; Sigma-Aldrich) for five consecutive days ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioning was performed on groups of around 40-50 flies with 3-octanol (around 95 % purity; Sigma-Aldrich) and 4-methylcyclohexanol (99 % purity ...
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bioRxiv - Molecular Biology 2022Quote: ... brain from one mouse from each group were removed under anaesthetized condition (by ketamine-xylazine cocktail, Sigma-Aldrich). Mice brains were placed in dimethyl formamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Pooled cells from 3 experimental replicates per group were lysed in lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... specimens (n=8 from each group) were sacrificed using a lethal dose (400ppm) of tricaine (MS-222; Sigma) and immediately imaged under light microscopy at 20x before rigor mortis set in to maintain flexibility in the jaw joints ...
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bioRxiv - Neuroscience 2023Quote: Five-month-old C57BL/6 mice (13/group; 10 male and 3 female) were administered dexamethasone (D2915, Sigma; 5mg/kg per day ...
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bioRxiv - Cancer Biology 2023Quote: ... For RNA experiments 4 tumors of each experimental group were dissociated with collagenase IV (50 mg/mL) (Sigma) and a tissue chopper (Ted Pella ...
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bioRxiv - Neuroscience 2023Quote: ... two animals per group were intracardially perfused with 0.9% NaCl solution followed by 4% paraformaldehyde (PFA; Sigma-Aldrich) solution ...
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bioRxiv - Cell Biology 2023Quote: ... Gelatin was modified with methacryloyl groups by addition of 8.75mL (80 DOF) or 5.45mL (50 DOF) methacrylic anhydride (Sigma) dropwise to the 100 mL gelatin solution ...
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bioRxiv - Microbiology 2023Quote: ... A and B groups were orally inoculated with 0.2 ml 15 mg ml-1 ampicillin (Sigma-Aldrich, USA) twice daily to disorder the gut microbiota ...
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bioRxiv - Cell Biology 2024Quote: ... whole cell lysates were prepared from two pellets per group using Phosphosafe Extraction Reagent (Merck Millipore, Darmstadt, Germany) containing 1 mM Pefablock SC (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... CD1 male (n=10 per group) mice between the ages of 4-6 weeks were given tamoxifen (Sigma) in peanut oil ...
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bioRxiv - Immunology 2024Quote: ... Mice in the model groups received 10 µg of LPS (from Escherichia coli; Sigma-Aldrich, Merck Millipore, Germany) in 20 µl of sterile normal saline solution three times a week for 3.5 consecutive months ...
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bioRxiv - Immunology 2024Quote: ... Mice in the model groups received 10 µg of LPS (from Escherichia coli; Sigma-Aldrich, Merck Millipore, Germany) in 20 µl of sterile normal saline solution three times a week for 3.5 consecutive months ...
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bioRxiv - Neuroscience 2024Quote: ... Both groups were treated with WIN55,212-2 (1 nM or 10 nM) (Sigma-Aldrich, St. Louis, MO, USA) dissolved in ethanol ...
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bioRxiv - Pathology 2024Quote: ... the C16 treatment group mice received 10 µg/kg body weight of C16 (Sigma-Aldrich, St. Louis, MO) dissolved in 0.5% DMSO-PBS (10 ml/kg body weight) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2021Quote: ... sBHI media controls or Porphyromonas cell-free supernatants (240 µg of total protein) were incubated with 120 µg of human fibrinogen (Sigma-Aldrich, St. Louis, MO). Reaction mixtures were incubated at 37°C under anaerobic conditions or in an atmosphere of 5% CO2 for cell suspension or cell-free supernatants ...
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bioRxiv - Microbiology 2020Quote: ... Human serum-supplemented medium was made through addition of complete human serum (Sigma Aldrich) into CDM to a final concentration of 5% (v/v) ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in an equal volume of human serum from human male AB plasma (Sigma) and incubated for 16 h at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10 % heat-inactivated human serum from human male AB plasma (Sigma-Aldrich), 2.5 μM zoledronic acid (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: ... from HEK293 cells stably expressing p3XFLAG-ADCK4 or -BAP were incubated with 80 μL of FLAG M2 agarose beads (Sigma-Aldrich) for 48 h at 4°C in an orbital shaker ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Cell Biology 2021Quote: ... GBM#U3013 cells were infected with lentiviral particles generated by transfecting HEK293 cells with pLKO.1-puro plasmids from the Mission shRNA library (Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2021Quote: ... HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa, The Francis Crick Institute, UK) were maintained in DMEM (Sigma-Aldrich) containing 4.5 g/L glucose ...
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bioRxiv - Immunology 2022Quote: Luciferase-expressing HeLa or HEK293 cells were seeded into a U-bottomed 96-well plate plus D-Luciferin (75 μg/ml; Sigma-Aldrich) for cytotoxicity assessment ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were cultured in serum-free and phenol red-free media containing 4.5 g/L glucose (Sigma-Aldrich, Dorset, UK), 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: Cell lysate from HEK293 transfected with FLAG-Podocin (WT, R45A, R50 and R54A) was immobilized on anti-FLAG beads (Sigma-Aldrich). The beads were washed with IP buffer (1%–2% Triton-X ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2023Quote: ... To pcw-KRAS-G12D-blast vector was packaged into a viral assembly by co-transfecting with PMD2.G (addgene, 12259) and PSPAX2 (addgene,12260) into HEK293 T cells using polyethyleneimine (PEI) transfection reagents (Sigma 408727). The virus-containing medium was used to infect HPNE E6/E7/st40 cells with 8ug/ml polybrene ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
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bioRxiv - Bioengineering 2019Quote: Ni-NTA columns were prepared by packing 1 mL Ni-NTA His•Bind resins (Sigma) into a 6-ml ISOLUTE® Single Fritted Reservoir column with 10 μm polyethylene frit (Biotage ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was cleaved by overnight incubation with thrombin (from bovine plasma, Sigma Aldrich) at 4 °C during dialysis ...
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bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...