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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2024Quote: ... and confirmed by Western blotting using His-tag antibody (Sigma; SAB 1305538). The His-tagged GUN1-PS protein was produced essentially as described (19) ...
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bioRxiv - Immunology 2024Quote: ... and 5% Fetal bovine serum (FBS; Sigma Heat Inactivated, US/HI origin). PBMCs were cultured overnight in chemotaxis media containing RPMI and 1% ultrapure BSA prior to use in assays.
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich) at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... with 4g/L Ox-bile (Sigma, or 2 g/L Hi-Media), 4 mL/L Tween60 (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... another membrane was incubated with anti-His antibody (05-949, Millipore, USA) at 4°C for 12 h and then incubated with an IRDye 800 secondary antibody for 1 h ...
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bioRxiv - Pathology 2023Quote: ... was performed in a representative subset of our participants using an adapted version of the MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel (Millipore Sigma, Burlington, MA, USA). The MAGPIX® Luminex analyzer (Luminex Corporation ...
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bioRxiv - Immunology 2024Quote: ... and IFN-γ were measured in the full Generation R cohort using the Human High Sensitivity T-Helper Cells Custom 5-plex assay from Millipore (Millipore, St. Charles, MO, USA) on the Luminex™ 100 system (Luminex ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... or human liver microsomes (HLMs) (male human pooled, Sigma-Aldrich), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... high glucose (Sigma, D1152), 15% FBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... high glucose (Sigma-Aldrich), supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... High glucose) (Sigma, G5767) were dissolved in growth medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Human haemoglobin (Sigma) was used for creation of standard curve.
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bioRxiv - Microbiology 2021Quote: ... human insulin (Sigma), 10’000 units/ml of penicillin and 10’000 µg/ml streptomycin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... human HDL (Millipore), human LDLs (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... human LDLs (Millipore) or an in vitro reconstituted NS1-HDL mix were analyzed by size exclusion chromatography on a Superdex 200 10/300 column (GE healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Human hemoglobin (Sigma) was dissolved in PBS to 10 mg/mL or 1 mg/mL ...
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bioRxiv - Microbiology 2024Quote: ... Human DPP4 (Sigma) was used as a control in PBS at 50 ng/μL (0.585 μM) ...
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bioRxiv - Cancer Biology 2021Quote: ... MultiScreen® HTS 96 multiwell plates (pore size 0.45 μm) with high protein-binding membrane (hydrophobic Immobilon-P PVDF membrane) were purchased from Millipore (Amsterdam, The Netherlands), conical 96-well Nunc plates from Thermo ...
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bioRxiv - Microbiology 2023Quote: ... The HOS-TMPRSS2 cells that stably express Rhinolophus bat ACE2 were generated as described below (see “Generation of HOS-TMPRSS2 cells stably expressing a variety of ACE2 proteins” section) and maintained in Dulbecco’s modified Eagle’s medium (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
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bioRxiv - Neuroscience 2021Quote: The transfected HEK293 cells were seeded on glass coverslips (Matsunami) coated with poly-L-lysine solution (Sigma-Aldrich) then incubated for 4–10 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T and HEK293-FT cells were maintained in DMEM supplemented with 10% FBS,1 mM sodium pyruvate (Sigma), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: HEK293-FLP cells stably expressing UBC:DOR-APEX2 were plated onto poly-L-lysine (Sigma Aldrich, Cat # P8920-100ML) coated coverslips in wells of a 24-well plates ...
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bioRxiv - Microbiology 2023Quote: HeLa cells and HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (Sigma) at 37°C in 5% (V/V ...
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bioRxiv - Neuroscience 2024Quote: ... SH3BP2-Flag was expressed in Hek293 cell and purified using anti-FLAG agarose beads (A2220-5ML, Sigma-Aldrich) and eluted with FLAG peptide (F4799-4MG ...
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bioRxiv - Molecular Biology 2020Quote: ... a single fluorescent cell was sorted per well of a fibronectin-coated (10 μg/mL Human Plasma Fibronectin Purified Protein (Sigma Aldrich) in 1x PBS ...
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bioRxiv - Biochemistry 2023Quote: ... No. H7379) and human hemoglobin A0 (HbA0) (with 5% (w/w) methemoglobin) (Cat. No. H0267) (all protein samples from Sigma, USA). Tetrahydrofuran (THF ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
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bioRxiv - Neuroscience 2024Quote: Proteins from human cerebral cortex and whole mouse brain were extracted using RIPA buffer (MilliporeSigma) with Protease Inhibitor cocktail (Sigma P8340) as previously described [20] ...
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bioRxiv - Cell Biology 2020Quote: ... free aldehyde groups were quenched with a solution of 1 mg/ml sodium borohydride (Sigma-Aldrich) freshly added to cytoskeletal buffer (10 mM MOPS ...
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bioRxiv - Developmental Biology 2021Quote: ... Group 2 (E2) was cultured with ExM containing 10 nM beta-estradiol (Sigma, Cat. No. E8875) for 6 days ...
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bioRxiv - Developmental Biology 2019Quote: ... The first group was given an intraperitoneal injection (IP) of SR11237 (Sigma, Oakville, Ontario, Canada, #S8951) (pan-RXR specific agonist ...
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bioRxiv - Biophysics 2021Quote: ... The bottom coverslip was functionalized with an amino-group in the 2% 3-aminopropyltheithoxysilane (440140, Sigma) in acetone for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... pRS-1-treated-diabetic (group-IV) and the positive control-treated with commercial ECM (Matrigel, Sigma) diabetic-models (group-V).
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bioRxiv - Cell Biology 2021Quote: ... Both groups were euthanized using buffered 2.64 g/mL MS222 tricaine mesylate (Sigma-Aldrich Oakville, Canada) at the end of treatment ...
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bioRxiv - Neuroscience 2022Quote: ... DG tissues from mice of each group were homogenized in RIPA buffer (Sigma, St. Louis, MO) with a cocktail of phosphate inhibitors and then centrifuged at 10,000 x g for 20 min at 4° C ...
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bioRxiv - Physiology 2020Quote: ... They were divided into two groups – Control and RTX (Resiniferatoxin~95%; Sigma-Aldrich, Missouri, United States) (n=3 each) ...
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bioRxiv - Microbiology 2020Quote: ... mice in DFOA group received oral gavage of Deferoxamine 100 µM 200ul (DFOA: Sigma, cat# 1166003), mice in AMX+DFOA received both AMX and DFOA ...
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bioRxiv - Microbiology 2020Quote: ... mice in AMX group received oral gavage of Amoxicillin 50 mg/kg (AMX: Sigma, cat# 1031503), mice in DFOA group received oral gavage of Deferoxamine 100 µM 200ul (DFOA ...
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bioRxiv - Cell Biology 2019Quote: ... The control group was treated with Mission siRNA Universal Negative Control # 1 (Cat# SIC001, Sigma-Aldrich). Gene silencing was done based on a previously published protocol (26 ...
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bioRxiv - Biochemistry 2022Quote: ... for SILAC “heavy” group and the same bead bed volume of pre-equilibrated Sepharose (Sigma, #4B200) in HAC buffer (10 mM phosphate buffer pH 7.4 with 50 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... while the experimental group received oral administration of vitamin E (Sigma-Aldrich; Burlington, MA, USA; 258024) (500 mg/kg of body weight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Two control groups were used: (a) Distilled water and (b) ivermectin (5 mg/mL, Sigma-Aldrich). A ...