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Citations for Millipore Sigma :
8301 - 8350 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... With the fluorescent GCase substrates blue fluorogenic substrate 4-methylumbelliferyl-β-D-glucopyranoside (4-MU) (Sigma-Aldrich; as described in 11,12), PBMCs were washed 2x with PBS and lysed in 60μl of activity assay buffer (50mM Citric acid ...
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bioRxiv - Neuroscience 2022Quote: ... where they were lightly anesthetized with isoflurane (to reduce stress from injection) and intraperitoneally injected with 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) at a dose of 50 mg/kg ...
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bioRxiv - Immunology 2022Quote: ... 10 µl of hemolymph was mixed with 90 µl of 3, 4-Dihydroxy-L-phenylalanine (L-DOPA, 4 mg/ml)(Sigma Aldrich) dissolved in nuclease free water (Cytiva) ...
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bioRxiv - Immunology 2022Quote: Itaconate (ITA) and its derivatives 4-octyl itaconate (4-OI) and Dimethyl itaconate (DMI) were purchased from Sigma-Aldrich (Deisenhofen, Germany) (ITA ...
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bioRxiv - Neuroscience 2019Quote: ... ubi:Switch double transgenic fish were immersed either for ten minutes in fish water containing 0.5 μM 4-hydroxytamoxifen (4-OHT - Sigma-Aldrich, T176) for clonal recombination of the reporter construct or 7 hours per day ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and post-fixed for at least 1 day in 4% PFA at 4°C and transferred to 30% sucrose (Millipore Sigma) for at least 1 day at 4°C ...
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bioRxiv - Cell Biology 2021Quote: EMT was induced in the HTER and HSER cell lines by chronic stimulation with 4 ng/ml 4-hydroxytamoxifen (4OHT; Sigma Aldrich) for 14 days9 ...
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bioRxiv - Neuroscience 2019Quote: ... with the following modifications: 4-day-old female adult brains were dissected in PBS and then fixed in 4% (wt/vol) paraformaldehyde (Sigma-Aldrich) in PBS on ice for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... TrkCCreERT2::Rosa26ChR2-YFP mice were treated with a single intrathecal (i.t.) injection of 45 ng of 4-hydroxytamoxifen (4-OH Tamoxifen, Sigma Aldrich, H7904). Experiments were performed at least one week after the treatment.
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bioRxiv - Cell Biology 2020Quote: ... pH 7.4) for 1 hour at room temperature and then incubated at 4°C overnight with primary antibodies (anti-multicillin, Sigma Aldrich, HPA058073 1:500 ...
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bioRxiv - Plant Biology 2020Quote: Spin trapping assays with the spin probe 4-pyridyl-1-oxide-N-tert-butylnitrone (4-POBN; Sigma-Aldrich, St. Louis, USA) to detect the formation of hydroxyl radicals24 were carried out using cyanobacterial cells (OD730 = 0.65 ...
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bioRxiv - Microbiology 2020Quote: Phosphatase and lipase activity were measured by following the hydrolysis of fluorescent substrates 4-Methylumbelliferyl phosphate and 4-Methylumbelliferyl oleate (both from Sigma-Aldrich) following standard methods (Hoppe ...
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bioRxiv - Molecular Biology 2020Quote: ... The fraction was incubated for 4 hours at 4°C with 250 μl of anti-FLAG M2 affinity resin (Sigma-Aldrich) equilibrated with lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... obtained from the NA activity assay of the PV to a standard curve of 4-Methylumbelliferone sodium salt 4-MU(SS) (Sigma M1508). NA activity is based on the production of 4-MU over time (60 minutes at 37 °C for the standard assay) ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was incubated for 4 hrs at 4 °C with 250 µL of anti-FLAG M2 affinity resin (Sigma-Aldrich) equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: NAD total levels in MT-4 cells and MT-4 cells infected by HIV-1 were assessed using the NAD/NADH Quantification Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... onto 4-inch silicon (Si) wafers previously coated with a sacrificial layer in poly(4-styrene-sulfonic acid) (PSS; 561223, Sigma Aldrich). Thiol-ene photopolymerization was performed under UV light (365 nm ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
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bioRxiv - Evolutionary Biology 2022Quote: ... erythrogramma embryos were fixed for in-situ hybridization (ISH) for ∼16 hrs overnight at 4 °C in 4% paraformaldehyde (Sigma 158127) + 20 mM EPPS (Sigma E1894 ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were seeded in 15-well Ibidi chambers after passaging and fixed 3-4 days later in 4% Paraformaldehyde (Sigma-Aldrich) for 60 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged (500g at 4°C for 4 minutes) and resuspended in PBS containing 1 μg/mL DAPI (Sigma Aldrich, D9542-100MG). Analysis of flow cytometric data was preformed using the BD Fortessa X20 and FlowJo v10 software (Ashland) ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... The FLAG-tagged WT sCTLA-4 or Y139A sCTLA-4 in the culture supernatant was purified with the anti-FLAG M2 affinity gel (Sigma-Aldrich), washed with wash buffer (Phosphate buffered saline ...
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bioRxiv - Molecular Biology 2023Quote: ... Newly synthesized RNA was then labelled by incubating the cells for 1h with 100 µM 4-thiouridine (4-sU, Sigma-Aldrich). RNA extraction was performed with peqGOLD TriFast Reagent (VWR ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested and pelleted by centrifugation for 4 minutes at 800 x g and washed twice in vPBS before being resuspended in fixation solution consisting of 4% formaldehyde and 4% acrylamide and transferred to a 12 mm round coverslip (Sigma, P4707) coated in poly-L-lysine and placed in a 24 well plate ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... in combination with 150 μg/ml heparin (Gilvasan, 1000 IE/ml) or 40-μM 4-aminobenzoic acid hydrazide (4-ABAH) (Sigma-Aldrich) where applicable.
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bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 28 d at 4°C ...
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bioRxiv - Immunology 2023Quote: ... melanoma was induced by injecting B6.Cg-Tg(Tyr-cre/ERT2)13Bos BRAFtm1Mmcm Ptentm1Hwu/BosJ (BRAFV600E PTEN-/- Tyr::CreERT2, Strain #013590) mice subdermally with 4-hydroxytamoxifen (4-HT)(Millipore Sigma, H6278 ...
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bioRxiv - Neuroscience 2023Quote: ... and fixed overnight at 4°C in phosphate buffer saline solution (PBS) containing 4% (vol/vol) paraformaldehyde (cat# P6148, Sigma-Aldrich). Layers of tissue containing the myenteric plexus were separated by microdissection ...
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bioRxiv - Bioengineering 2023Quote: ... or 5 µg/mL NETs for 4 hr prior to fixation with 4% paraformaldehyde (Electron Microscopy Services, Hatfield, PA) in phosphate-buffered saline (PBS, Sigma-Aldrich) for 15 min at room temperature and staining with 0.005% Crystal Violet in PBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 15,000 × g for 15 min at 4 °C and were incubated for overnight at 4 °C with anti-FLAG M2 antibody (F1804, Sigma-Aldrich). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... On the 7th day 1 µM Senexin B (SenB, Senex Biotechnology, Columbia, SC) or 1 µM 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... at 6-8 weeks of age via intraperitoneal injections (100 μL of 4 mg/mL solution per day for 4 consecutive days) dissolved in corn oil (Sigma, C8267). All mice were used in mixed genetic backgrounds ...
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bioRxiv - Neuroscience 2024Quote: ... In Aldh1l1-CreER;mGluR5fl/fl;GCaMP6s and Aldh1l1-CreER;mGluR3fl/fl;GCaMP6s mice (Z)-4-Hydroxytamoxifen (4-OHT; H7904; Sigma-Aldrich) was used ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed for 1 day in 4% PFA at 4°C before transferring to 30% sucrose (Millipore Sigma) in PBS at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... the density of amines on the surface of the coverslips was quantified using UV absorbance of 4-nitrobenzaldehyde (4-NB, Sigma Aldrich) attached to amines on functionalized coverslips as we have reported elsewhere.[31] Calibration curves of absorbance of 4-NB were generated reading spectra from a UV-Vis Spectrophotometer (Varian Cary 50 Bio) ...
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bioRxiv - Immunology 2024Quote: ... The filtered supernatant was then concentrated using a centrifugal filter unit with a 3 kDA cutoff at 4°C (Amicon Ultra-4 Centrifugal filters, Millipore, USA). Concentrated supernatant was stored in 200 µL aliquots at −80°C until further analysis.
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bioRxiv - Plant Biology 2020Quote: ... 5 μg anti-FLAG antibody (Sigma, F3165) per gram of tissue were added to the supernatant in a new tube and incubated at 4°C overnight ...
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bioRxiv - Biophysics 2020Quote: ... 5 % (v/v) glycerol (Sigma Aldrich, G7757), and 1 mM Tris(2-carboxyethyl)phosphine (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μg/mL bovine insulin (Sigma I1882) and 1 μg/mL hydrocortisone (Sigma H0888) ...
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bioRxiv - Cell Biology 2019Quote: ... and 5’-UCGUGGAAAGUUUGCUGCAGGGAAA[dT][dT]-3’ (Sigma) (Doucet et al. ...
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bioRxiv - Cell Biology 2020Quote: ... carbonyl cyanide 5-chloro-phenylhydrazone (CCCP) (Sigma), PLD2 inhibitor VU 0364739 hydrochloride (Tocris 4171) ...
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bioRxiv - Cell Biology 2020Quote: ... For some experiments EPA (20:5) (Sigma) was mixed with PA and together conjugated to BSA.
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bioRxiv - Immunology 2021Quote: ... or 3-MA (5 mM, Sigma-Aldrich), as indicated in the figure legends ...
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bioRxiv - Immunology 2021Quote: ... or 5 μm MHY1485 (Sigma-Aldrich, USA) dissolved in DMSO and refreshed daily.
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bioRxiv - Molecular Biology 2021Quote: ... alkylated by 5 mM iodoacetamide (Millipore Sigma) for 30 min in dark at ambient temperature and digested by 5 μg/μL trypsin (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked in Goat Serum 5% (Sigma Aldrich) in PBST and incubated with primary antibodies at indicated concentrations overnight in Goat Serum 2% in either PBST (0.1% TritonX ...
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bioRxiv - Developmental Biology 2020Quote: ... 5 ng/mL IGF1 (Sigma 13769-50UG), 0.2 ng/mL TGFβ (R&D systems 240-B) ...
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bioRxiv - Genomics 2020Quote: ... 5 μL of PMSF (100 μM, Sigma) was added to the lysate and incubated for 10 minutes at room temperature to inhibit the activity of Proteinase K ...