Labshake search
Citations for Millipore Sigma :
8251 - 8300 of 10000+ citations for 5 Pyrimidinecarbonitrile 2 4 diamino 6 methyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... mice received 30 mg/l 4-deoxypyridoxine (Sigma) combined with 1 g/L sucrose or 1 g/L sucrose alone in the drinking water 3 days before IL-25 injection.
-
bioRxiv - Molecular Biology 2024Quote: ... by treatment with 500nM 4-hydroxytamoxifen (Sigma Aldrich).
-
bioRxiv - Pathology 2024Quote: ... cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 30 min ...
-
bioRxiv - Cell Biology 2020Quote: ... 25 mM Tris pH7.4, 5 mM EDTA, 0.5 mM DTT, 1% NP40, 5% glycerol and protease inhibitor cocktail from Sigma). Cells were lysed using a Bioruptor (Diagenode ...
-
bioRxiv - Microbiology 2019Quote: ... 1.5 mM CaCl2, 5 mM citric acid, 5.6 mM glucose, 10 mM NH4Cl, 5 mM H3PO4, pH 7.4; all Sigma). Cells were then incubated in choline-buffer with control medium or pyolysin for 5 min at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... and mouse VPS35 (5’-GAUUCGAGAAGAUCUCCCA[dT][dT]-3’&5’-GUAAUGUUCUGGAUUAUAA[dT][dT]-3’) were purchased from Sigma-Aldrich. At 24 h after transfection ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with 0.25% Triton X-100 for 5 min and blocked with 5% goat serum (Sigma) in PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... a pre-mixed solution of 5 µL 50 mM CuSO4 with 5 µL 250 mM THPTA (Sigma-Aldrich) was added ...
-
bioRxiv - Biochemistry 2022Quote: ... Hypotonic buffer (10 mM Tris-phosphate, 5 mM EDTA, 5 mM DTT, 0.2% Igepal CA-630 (Sigma Aldrich), pH 7.4 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked for 1 hour in either 5% milk or 5% Bovine Serum Albumin (BSA) (Sigma, A7906) diluted in PBS/0.2% Tween ...
-
bioRxiv - Neuroscience 2020Quote: ... by diluting in sterile vehicle (2.5mL 5% polyethylene glycol, 2.5mL 5% Tween-80, and 45mL sterile, deoionized water, filtered with Steriflip (Millipore)) ...
-
bioRxiv - Microbiology 2022Quote: ... log-phase cultures grown in YPD were stained for 5 min with 5 μg/mL of filipin (Sigma) and washed with PBS ...
-
bioRxiv - Molecular Biology 2019Quote: ... 5 minutes each with PBST at room temperature (RT) with agitation and blocked with 5% Milk Powder (Sigma) in PBST for 1 hr at RT with agitation ...
-
bioRxiv - Bioengineering 2020Quote: ... Following PBS washes (5 min x3) and overnight incubation in blocking buffer (5% bovine serum albumin (Merck Millipore) in PBS ...
-
bioRxiv - Developmental Biology 2020Quote: ... washed 5 min in 50% methanol/50% phosphate-buffered saline with 0.1% Tween-20 (9005-64-5, Sigma) (PBT) ...
-
bioRxiv - Biophysics 2021Quote: ... Chambers were further passivated for 5 minutes at room temperature with 5% Pluronic F-127 (P2443, Sigma-Aldrich) and extensively washed with assay buffer (80 mM PIPES ...
-
bioRxiv - Cell Biology 2020Quote: ... 5 mM DTT) and eluted with Buffer A supplemented with 5 mM DTT and 3 mM Desthiobiotin (SIGMA). Peak fractions were combined and further purified via size exclusion chromatography on a Superdex 200 (GE Healthcare Life Sciences ...
-
bioRxiv - Microbiology 2022Quote: ... A 1 mL sample of the bacterial culture was mixed with 5 µL 5 mM CaCl2 (Sigma-Aldrich), 100 ng of the rpoBS482F PCR product in 2 µL water ...
-
bioRxiv - Developmental Biology 2023Quote: ... Following transfer membranes were either blocked in 5% dried milk (Marvel) in PBST or 5% BSA (Sigma Aldrich). Membranes were incubated with primary antibodies (Supplementary Table 1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were permeabilized with PBS containing 0.5% Triton-X for 5 min and blocked with 5% BSA (Sigma) for 1 h prior to staining with indicated primary antibodies for 1 h at RT ...
-
bioRxiv - Neuroscience 2024Quote: ... Absolute and relative activity for the full 24-hour recording and 12-hour periods were also calculated for six different frequency bands (i.e., SWA 0.5-5 Hz, Theta 5-9 Hz, Alpha 9-12 Hz, Sigma 12-16 Hz ...
-
bioRxiv - Genomics 2023Quote: ... The selected drugs and concentrations used in the experiment were as follows: 5 µM 5-azacytidine (A2385, Sigma), 10 µM dexamethasone (D1756 ...
-
bioRxiv - Biochemistry 2024Quote: ... 50 mM NaCl and 5% glycerol pH 7.4) supplemented with freshly added 5 mM desthiobiotin (Millipore Sigma, D1411) to form “EB” ...
-
bioRxiv - Molecular Biology 2024Quote: ... For zC-FISH-PLA experiments cells were incubated with 5 μM of 5-Azacytidine (zC, Sigma-Aldrich #A2385) for 1h at 37°C before incubation of 400nM (Z)-4-Hydroxytamoxifen (4OHT ...
-
bioRxiv - Cell Biology 2020Quote: ... 50 ml of 2% L-cysteine solution (2 g L-cysteine (Sigma Aldrich, #168149-100G) in 100 ml 0.1% MMR ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 150 μL 2× Laemmli SDS-PAGE loading buffer supplemented with 10% 2-mercaptoethanol (Sigma-Aldrich) were added ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2-HG production was assessed via D-2-Hydroxyglutarate (D2HG) Assay Kit (Sigma-Aldrich, USA). Paired GBM cell lines were also treated with hit inhibitors individually and in combination ...
-
bioRxiv - Neuroscience 2021Quote: ... a selective neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) hydrochloride (Sigma, Dorset, UK) was used 3,4 ...
-
bioRxiv - Neuroscience 2022Quote: ... 2 DIV differentiated cells were treated with 1 µM CDK1/2 inhibitor III (Millipore, 217714) in control medium supplemented with 2% FBS for 24 h ...
-
bioRxiv - Bioengineering 2021Quote: ... We added 2-amino-thioxanthone (17.2 μmol, 2 equ) in dimethylsulfoxide (DMSO, 0.1 mL, Sigma) to a solution of PA-Rho (8.6 μmol ...
-
bioRxiv - Molecular Biology 2020Quote: ... Transduced organoids were selected with 2 rounds of 2 μg/ml of puromycin (Sigma-Aldrich) on day 2 and day 4 ...
-
bioRxiv - Biochemistry 2021Quote: ... and incubated with the 0.1mM substrate methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (Sigma) for 1h at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then pre-treated with 2mM 2-deoxy-D-glucose (2-DG) (Sigma-D6134) for 15 minutes prior to the assay ...
-
bioRxiv - Developmental Biology 2022Quote: ... 2.5 μM Oligomycin A (BioShop) or 50 mM 2-Deoxy-D-glucose (2-DG, Sigma) for 24 hours ...
-
bioRxiv - Physiology 2022Quote: ... 0.1 M N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) sodium salt (Sigma-Aldrich, H7006), 0.12 M sodium chloride (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
-
bioRxiv - Immunology 2023Quote: ... these cells were pre-treated or not with 2-bromopalmitate (2-BP, Sigma, 21604-1G) at 50 μM for 30 min ...
-
bioRxiv - Physiology 2023Quote: ... For sectioned tissues (Figure 2, Supplementary Figure 2) samples were incubated in 30% sucrose (Sigma) overnight (for brains 5 days) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Triplicates of bacterial overnight cultures in Mueller-Hinton broth 2 (MHB 2, Sigma-Aldrich, #90922) were prepared from three different colonies the day before and grown shaking at 37 °C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Lineage-labeling was induced at indicated time points using fresh or pre-heated (65°C for 10 min) 4-Hydroxytamoxifen (4-OHT) (Sigma H7904) stock solutions in DMSO at a final concentration of 10 μM in E3 embryo medium (Felker et al. ...
-
bioRxiv - Developmental Biology 2021Quote: Recombination of Col1a2xLPCherry-Tg was induced by keeping animals in separated tanks with water containing (Z)-4-hydroxytamoxifen (4-OHT, Sigma T5648) 2 µM overnight ...
-
bioRxiv - Genetics 2019Quote: ... 1000 μg whole cell lysates were incubated with the indicated anti-Flag M2 affinity gel and monoclonal anti-HA agarose for 3-4 hr at 4 degree (Millipore Sigma). Immunoprecipitants were washed five times with NETN buffer (20 mMTris ...
-
bioRxiv - Biochemistry 2019Quote: ... Freshly purified CsgA was filtered using a 30 kDa cut-off column at 4°C (Amicon® Ultra-4, Sigma-Aldrich) to remove insoluble protein aggregates and seeds ...
-
bioRxiv - Genetics 2020Quote: ... cells were fixed at day 4 of differentiation with 4% paraformaldehyde for 20 minutes at 4°C followed by permeabilization with 0.1% triton™X-100 (Sigma Aldrich) for 30 minutes at room temperature and blocking in 1xPBS with 1% fetal bovine serum (Gibco) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: For multiphoton microscopy kidneys were fixed in 4% paraformaldehyde overnight at 4 °C and embedded in 3% low-melting point agarose (Sigma- Aldrich). Sections (500 µM ...
-
bioRxiv - Plant Biology 2021Quote: ... roots of Arabidopsis thaliana Col-0 and sr45-1 mutant seedlings (or 9-week-old plants) were vacuum infiltrated with HCl (4%, v/v) and K-Ferrocyanide (II) (4%, w/v, Sigma-Aldrich) (1/1 ...
-
bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 56 d at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1×106 cells were fixed overnight in a solution containing 4% formaldehyde and 4% acrylamide in PBS and adhered to a poly-L-lysine (Sigma, P4707) coated coverslip ...
-
bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
-
bioRxiv - Cancer Biology 2021Quote: ... were used to infect subconfluent MCF7 KRT19 KO cells in three sequential 4 h incubations in the presence of 4 μg/ml polybrene (Sigma-Aldrich). Transductants were selected in hygromycin (100 μg/ml) ...