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Citations for Millipore Sigma :
8201 - 8250 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and a thrombin solution containing CaCl2 and aprotinin (final concentration of fibrin components: 6 mg/mL fibrinogen (Sigma-Aldrich); 2 NIH U/mL thrombin from human plasma (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... and pre-cultivated on MS1 medium[MS0 with 0.1 g/L IAA (Sangon Biotech Co., Ltd, Shanghai, China) and 1.5 g/L 6-BA (Sigma, USA)] ...
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bioRxiv - Microbiology 2020Quote: ... and naïve CD4+ T cells (6 × 104 cells/well) were plated together with 0.1 nM retinoic acid (Sigma-Aldrich) and 2 ng/ml recombinant mouse TGF-β (R&D Systems) ...
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bioRxiv - Microbiology 2020Quote: ... The epithelial cell proteins that remained bound to the fungal cells were eluted twice with 6 M urea (Sigma). The proteins were then separated on 10% SDS-PAGE and transferred to immun-Blot PVDF Membrane (BIO-RAD) ...
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bioRxiv - Cancer Biology 2020Quote: ... MD2G (3 μg) and pLKO-LV-gene-specific (6 μg) plasmids in the presence of PEI transfection reagent (Sigma). The generated lentiviral particles were collected 24 hours post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Rose-John), 20 U/ml recombinant murine GM-CSF (PharmedArtis, Germany) and 10−6 M dexamethasone (Sigma-Aldrich, USA). Progenitor cells were amplified 5 to 11 days before differentiation into DC was induced by supplementing 200 U/ml recombinant murine GM-CSF ...
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bioRxiv - Genetics 2022Quote: ... one million cells were seeded in 6-well plates and co-transfected with 1μg of total DNA using the ESCORT IV transfection reagent (Sigma) following the instructions of the manufacturer ...
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bioRxiv - Genetics 2022Quote: ... Three days later 200 μl of the transfected cells were seeded into new 6-well plates treated with 0.01% poly-L-Lysine (Sigma), followed by selection with 1mg/ml of Zeocin (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... filtered through 0.45 mm filters and concentrated to 6 mL in Amicon Ultra 100K centrifugal filter units (Millipore Sigma). Concentrated lentiviral particles were used immediately or stored at -80°C.
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bioRxiv - Neuroscience 2022Quote: ... The skull was exposed and a burr hole was drilled to introduce a syringe for a single unilateral MFB injection of either vehicle (0.2mg/ml ascorbic acid in 0.9% sterile saline; pH5.0) or 6-OHDA (hydrobromide salt (Sigma Chemicals, UK); 3μg/μl in vehicle) ...
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bioRxiv - Neuroscience 2022Quote: ... a total of 60 male (eight-week-old) C57BL/6 mice were treated with streptozotocin (STZ, Sigma-Aldrich, USA) injection at a dose of 50 mg/kg for 5 consecutive days ...
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bioRxiv - Genomics 2022Quote: ... in TC-treated 6-well plates coated with 0.05 mg/mL Poly-D-Lysine (Sigma Aldrich A-003-E).
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bioRxiv - Biochemistry 2022Quote: ... Cells were plated at a density of 200,000- 300,000 cells per well in a 6-well plate in the presence of Polybrene transfection reagent (Sigma- Aldrich) and infected with 10% of the total virus from one 10 cm plate of the indicated constructs ...
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bioRxiv - Microbiology 2020Quote: ... the cells were transfected with 6 μg of pCG1_SARS-2_S using X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich). Twenty hours later ...
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bioRxiv - Molecular Biology 2020Quote: ... MEFs were plated in 6- well plates (10,000 per well) and treated continuously with different doses of PARPi or cisplatin (Sigma) for 7 days ...
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bioRxiv - Neuroscience 2021Quote: ... and plated on sterile 6 or 24-well plates coated with poly-D-Lysine (sterile lyophilized 135 kD; Sigma) and 1mg/ml laminin ...
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bioRxiv - Neuroscience 2021Quote: ... They were then administered three consecutives intraperitoneal (i.p.) injections (6 mg/kg body weight) of the glutamate agonist kainic acid (KA) (Sigma) dissolved in 0.1 M PBS pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... the monolayers were fixed with 10% (v/v) neutral buffered formalin for at least 6 h (NBF, Sigma-Aldrich) and stained with 0.2% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... Switzerland) were dissolved in glacial acetic acid brought to pH 6 with the addition of sodium hydroxide (NaOH, Sigma). Haloperidol (1mg/kg) ...
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bioRxiv - Biochemistry 2021Quote: ... were mixed with equal volume of 6 M guanidine hydrochloride solution and desalted by using ZipTip C18 (Millipore, USA). The protein was extensively washed with 0.2% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... the plates were incubated for 6 days at 37°C and were then stained with 0.2% (w/v) crystal violet (Sigma) in 2% ethanol for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and seeded at a density of 2 × 106 cells per well (6-well plates) or 0.5 × 106 cells per well (24-well plates) into PDL/Laminin (Sigma)-coated plates with neuronal medium plus 0.5 μM RA ...
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bioRxiv - Neuroscience 2022Quote: ... HeLa cells or iPSC-derived motor neurons grown on 6-well plates were rapidly rinsed with ice-cold PBS and subjected to ATP extraction with 0.5% TCA (Sigma). After incubation for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: Immunofluorescence (IF) was performed in tissue sections (6-8mm) blocked with 10% horse serum and 0.3% TritonX-100 (Sigma) for 1 hour at room temperature (rt) ...
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bioRxiv - Developmental Biology 2022Quote: ... were obtained from previous studies (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747754/) cultured in E14 plus complete medium (Glasgow Minimum Essential medium(Sigma-Aldrich G5154-6) supplemented with 10% heat inactivated FBS (Fisher Scientific SH3007103) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were infected with the lentiviruses in the presence of 6 μg/mL polybrene (Sigma-Aldrich, Cat. # H9268) for 24 h.
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bioRxiv - Developmental Biology 2022Quote: ... serum-LIF-grown ESCs were split with accutase onto laminin-coated 6-well/plates (Sigma, 10μg/ml in PBS) at a density of 0.75×104 cells/well in plain N2B27 medium ...
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bioRxiv - Bioengineering 2022Quote: ... and a medium with an extracellular signal-regulated kinase inhibitor (ERKi, MilliPore Sigma #1049738-54-6, Burlington, MA, USA), adapted from Eigler et al.2 More details on the media used are provided in Supplementary Table 1.
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bioRxiv - Biochemistry 2019Quote: HEK293T shHuR/shAUF1 and shControl cells (9.6 × 105 cells) in 6-well plates were treated with 5 μg/mL actinomycin D (Sigma) and harvested in Trizol as described above at the indicated time points ...
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bioRxiv - Plant Biology 2019Quote: ... From the working solution an assay buffer was made adding 50U of Glucose-6-Phosphate Dehydrogenase (G6PDH; Sigma-Aldrich), and 295µL or 280µL of the working solution was added to a 96 well plate (Costar ...
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bioRxiv - Developmental Biology 2020Quote: ... We added potassium phosphate buffer pH 6 to the samples to a final concentration of 0.1 M and trehalase enzyme (SIGMA) to 1.5 U/ml ...
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bioRxiv - Cell Biology 2020Quote: ... The reaction was initiated by the addition of 6 μM vitamin D binding protein [DBP] (Human DBP, G8764, Sigma) directly in the fluorometric cuvettes ...
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bioRxiv - Immunology 2020Quote: ... 50 μl of antibody exposed to hemin or hemin in PBS only were mixed with 150 μl of reaction buffer (0.15 M citrate-phosphate buffer pH 5) containing 0.9 mM of 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma-Aldrich) and 6 mM H2O2 ...
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bioRxiv - Plant Biology 2021Quote: ... For Co-IPs with MED10B-MEC-ProtA we used IgG Sepharose® 6 Fast Flow (Sigma, GE17-0969-01) beads and increased washing steps (1xIP buffer ...
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bioRxiv - Genomics 2019Quote: ... Antigen unmasking was performed boiling sections on 10 mM citrate buffer pH 6 for 10min and tissue was permeabilized with PBS 0.5% Triton X-100 (Sigma). Blocking was performed by 1h incubation in humid chamber with 3% of normal donkey serum (Vendor ...
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bioRxiv - Immunology 2020Quote: ... one 10-cm dish of packaging cell line at roughly 70% density was transfected with the target construct and two supplementing vectors providing the VSV gag/pol and VSV-G-env proteins (6 μg of DNA each) using polyethylenimine (22.5 μg/ml, Sigma) and Polybrene (4 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stimulated for 6 h with PMA (10 ng/mL) and ionomycin (500 ng/mL) (both from Sigma), in the presence of GolgiStop plus GolgiPlug (both from BD Biosciences).
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bioRxiv - Immunology 2020Quote: ... Fractions containing the complex were concentrated to 6 mg/mL using a 10 kDa Amicon® spin concentrator (Millipore). 3 μL of the complex was mixed with 1 μL of a n-Dodecyl-β-D-Maltopyranoside (DDM ...
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bioRxiv - Neuroscience 2019Quote: ... The oxygen electrodes were calibrated in air-saturated 0.9% sodium chloride (at 37°C) and oxygen-free standard solution [0.1M sodium hydroxide (SX0607H-6, Sigma-Aldrich) and 0.1M sodium ascorbate (A7631 ...
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bioRxiv - Biophysics 2019Quote: ... freshly purified tau was first reduced with 1 mM DTT for 10 minutes and buffer exchanged into a labeling buffer (20 mM Tris pH 7.4, 50 mM NaCl, and 6 M guanidine HCl) using Amicon concentrators (Millipore) (17) ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were rinsed four times with cacodylate buffer and post-fixed with 0.8% K3Fe(CN)6 (Sigma-Aldrich) and 1% OsO4 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... BL cells were spun down and plated at 0.5 × 10^6 cells in fresh media containing soluble anti-IgM or anti-IgG (Sigma) at concentrations and times indicated in figure legends (2.5-5 ug/mL for 22-48 hrs).
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bioRxiv - Microbiology 2021Quote: ... all water samples were kept on ice for approximately 6 hours and filtered through a 0.22 μm Sterivex GV filter (Millipore) using a Masterflex L/S 7553-70 peristaltic pump ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cells grown on 6-well culture plates using lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... a pool of T cells was stimulated for 6 hours with 50ng/ml PMA and 1μg/ml Ionomycin (Sigma). T cells were then harvested and washed with FACS Buffer and stained for surface markers followed by fixation and permeabilization using BD Cytofix/Cytoperm Fixation/Permeabilization Solution (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: ... Cells were plated at a density of 0.8 × 106 cells per well on 6-well plates pre-coated overnight with 0.1 mg/ml poly-D-lysine (Millipore) and one third of the media in each well was replaced every 3 days ...
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bioRxiv - Biophysics 2020Quote: Immunoprecipitation samples were fractionated on 6% or 15% SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (PVDF) (Millipore). Membranes were blocked in TBS solution (0.05% Tween-20 in PBS containing 5% milk) ...
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bioRxiv - Cell Biology 2020Quote: Mitochondria were isolated from a total of ~60 million (30×6-well) cells by manual cell homogenization in a specialised buffer (20 mM HEPES (pH 7.6) (Sigma), 220 mM D-mannitol (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... > 6-weeks old mice were injected with 100□μl of tamoxifen (10□mg /□ml in corn oil) (Sigma, #T5648) via intraperitoneal (i.p. ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with shRNA-encoding lentivirus supernatants in the presence of 6 μg/ml polybrene (hexadimethrine bromide; Sigma-Aldrich). Twenty-four hours after infection ...