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Citations for Millipore Sigma :
8151 - 8200 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Mice were subjected to cutaneous immune challenge by applying 20 µl of 3-5% 4- Ethoxymethylene-2-phenyl-2-oxazolin-5-one (Sigma-Aldrich) in 1:2 acetone:olive oil ...
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bioRxiv - Immunology 2020Quote: ... Cryptococcus was grown for 18 hours overnight in 2 ml yeast peptone dextrose (YPD 50 g/L Fisher, 2% agar Sigma-Aldrich) medium ...
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bioRxiv - Cell Biology 2021Quote: ... LECs and imLECs were starved in serum starvation medium (EBM-2 with hydrocortisone, ascorbic acid, GA-1000 all at proprietary concentrations and 2% bovine serum albumin (BSA, Sigma-Aldrich)) for 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4° C with the following primary antibodies in 1X PBS containing 2% Triton X-100 and 2% horse serum: mouse anti-NeuN (EMD Millipore), rat anti-Ki-67 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... cultured for 24 hr in regular ES cell media followed by 2 days of selection using 2 μg/ml puromycin (Puro) (Sigma-Aldrich) and collected for subsequent analyses ...
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bioRxiv - Immunology 2020Quote: Expanded T cell lines were stimulated for 5h at 37°C with or without SARS-CoV-2 peptide pools (2 μg/ml) in the presence of 10 μg/ml brefeldin A (Sigma-Aldrich). Cells were stained with the yellow LIVE/DEAD fixable dead cell stain kit (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... 300 mM NaCl, 0.5% Nonidet P-40, 2 mM EDTA, 2 mM MgCl2, 0.5 M urea and protease inhibitors; Sigma; cat# P-8340) followed by centrifugation at 4°C for 20 min at 16,100 × g ...
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bioRxiv - Biochemistry 2022Quote: ... Proteolysis was stopped by mixing equal volumes of lysis buffer supplemented with 2 mM PMSF and 2 μg/mL CLAP (chymostatin, leupeptin, antipain and pepstatin (Sigma-Aldrich)) with the lysates ...
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bioRxiv - Immunology 2022Quote: Mice were sensitized on the shaved back-skin for two consecutive days with 50 μl of 5% Oxa (4-Ethoxymethylen-2-phenyl-2-oxazolin-5-on, Sigma-Aldrich) diluted in methanol and acetone (1:1 ...
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bioRxiv - Neuroscience 2023Quote: ... BP151-500) in 1X PBS then blocked for 2 hours at RT with 2% bovine serum albumin (BSA, Sigma-Aldrich, A2153), 0.1% Triton in 1X PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Albumin and Lipocalin-2/NGAL values were obtained using MILLIPLEX MAP Rat Kidney Toxicity Magnetic Bead Panel 2 (Millipore Sigma, USA) with 1:500 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... 2.71 g of CaO (calcined Ca(OH)2 (Jost Chemical Co., USA)) and 0.438 g of Sr(NO3)2 (Sigma-Aldrich, Germany) are mixed in deionized water (300 mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice with PBS + 2% FBS and then resuspended in 400 μL PBS + 2% FBS containing DAPI (Millipore Sigma, D9542 ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary cells of BC tissues were concentrated in six-well plates coated with 2% poly-2-hydroxyethyl methacrylate (Sigma-Aldrich) at a density of 1×104 cells/ml in serum-free DMEM/F12 (Gibco ...
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bioRxiv - Immunology 2023Quote: Yp was cultured to stationary phase at 28°C and 250 rpm shaking for 16 hours in 2×YT broth supplemented with 2 μg/ml triclosan (Millipore Sigma). Mice were fasted for 16 hours and subsequently inoculated by oral gavage with 200 μl phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS + 0.5% BSA + 0.1% sodium azide + 2% Normal Mouse Serum (NMS) and 2% FBS (Sigma, St. Louis, MO). Rat anti-mouse purified CXCR5 (2G8 ...
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bioRxiv - Neuroscience 2023Quote: ... male (N = 2) and female (N = 2) naïve mice were infused with Fluorogold (Sigma, St. Louis, MO; 4% in sterile saline) in the DMS (0.2 µL) ...
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bioRxiv - Immunology 2023Quote: ... Where indicated, glucose-free cultures were hexose-supplemented using D-glucose (2 g/L) (Fisher, D-galactose (2 g/L) (Sigma-Aldrich), or D-mannose (125 µM ...
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bioRxiv - Neuroscience 2023Quote: ... coverslips containing cultured DRG neurons were transferred to a new 35mm dish containing 5µM Fura-2- acetoxymethyl ester (Fura-2 AM) (Millipore Sigma 344905) in complete neurobasal media and placed in a 37°C + 0.5% CO2 incubator for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... for 15 min and blocked for 2 hours at 25 °C or overnight at 4 °C using 2% BSA (Sigma-Aldrich). Samples were incubated with respective primary antibodies at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma], and 0.1% Tween-20 [P9416; Sigma] in PBS) for 45 min ...
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bioRxiv - Plant Biology 2024Quote: ... Kit (Sigma). RNA was treated with TURBO DNAse (Thermo ...
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bioRxiv - Biophysics 2021Quote: ... an enzyme mixture was made with 0.1 µM GOx-488 and 0.1 µM GOx-532 in a 50 mM MES (pH 6; Sigma-Aldrich) buffer solution ...
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bioRxiv - Biophysics 2021Quote: Cells were grown in 6-well plates in RPMI1640 or DMEM media supplemented with 10% (v/v) FBS (Sigma), 2 mM glutamine (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre-soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Cell Biology 2020Quote: ... Three-month-old C57BL/6 male mice were injected with 10 µg BrdU (Sigma-Aldrich, St. Louis, MO; www.sigmaaldrich.com)/gram body weight intraperitoneally every 12 hours for 2 days before sacrifice ...
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bioRxiv - Cell Biology 2020Quote: ... and (1uM) Clemastine Fumarate D3:D20 (Cayman) for 6 weeks (M2; the optimal media) and (2.5ug/ml) Laminin (Sigma), and (1 ug/ml ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...
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bioRxiv - Microbiology 2022Quote: ... the larvae were kept in 6-well plates containing egg water (60 μg/mL sea salts (Sigma-Aldrich; S9883) in demi water ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated at a density of 6×104 cells / cm2 on glass coverslips coated with 0.2mg/ml Poly-L-lysine (#P2636-100MG Sigma) made in Borate buffer pH 8.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The neurons were quantified and centrifuged at 1,800 rpm for 6 min and then plated on the glass coverslips pre-coated with poly-L-lysine (Sigma). The culture medium was Neurobasal (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Reconstituted complexes were purified over a Superose 6 increase 3.2/300 size exclusion column SEC in GF150 buffer and immediately crosslinked in 0.025% Glutaraldehyde (Sigma-Aldrich) for 30 minutes on ice followed by quenching with 1 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Physiology 2019Quote: ... The study included five treatment groups (n≥6 per group) receiving either 0.24 g/kg bodyweight bovine fetuin-A (Sigma) or 0.10 g/kg bodyweight PPi (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... and kinate receptors was achieved with 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX; 50 µM; Sigma, Australia), N-methyl-D-aspartate (NMDA ...
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bioRxiv - Plant Biology 2019Quote: ... Fe(III)-cellulose (5 mM FeCl3 + 50 mM cellulose, pH 5.8, bought from Sigma, CAS number: 9004-34-6), Fe(III ...
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bioRxiv - Physiology 2019Quote: ... Eight-week-old male C57Bl/6 mice (Janvier Laboratories, France) were used for pharmacological experiments with spironolactone (Sigma-Aldrich), eplerenone (Inspra ...
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bioRxiv - Developmental Biology 2020Quote: ... resuspended in complete Neurobasal media and plated at 0.5 × 106 cells/well on 6-well plates coated with 0.1 mg/ml poly-L-lysine (PLL; Sigma Aldrich). Neurons were cultured at 37°C in a humidified incubator with 5% CO2 and medium replaced every 5 days with fresh medium containing B27.
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bioRxiv - Cancer Biology 2019Quote: ... cells were seeded in 6-well plates and transfected the next day with 30pmol of ESR1 (Sigma-Aldrich, SASI_Hs01_00078592), ESR2 (Dharmacon ...
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bioRxiv - Biochemistry 2019Quote: ... catalog number G1112 and an electron coupling reagent (PMS) [CAS: 299-11-6] from Sigma (St. Louis, MO,USA).
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bioRxiv - Biophysics 2019Quote: ... Dead cells were removed on day 6 after T cell isolation through density-dependent gradient centrifugation (Histopaque 1119, Sigma) and experiments were conducted between day 7 and day 9 after isolation.
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bioRxiv - Molecular Biology 2019Quote: ... male Wistar rats (n=5-6/group) were deeply anesthetized with sodium phenobarbital (Sigma-Aldrich; 50 mg/kg i.p.), and then positioned in a stereotaxic frame (TrentWells ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated with HDM or the known AhR agonist FICZ (5,11-Dihydroindolo[3,2-b]carbazole-6-carboxaldehyde, Tocris Bioscience, Bristol, UK or Sigma-Aldrich) to activate the AhR and induce CYP1A1 transcription ...
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bioRxiv - Plant Biology 2020Quote: ... Electrodes with fine tips (Resistance = 4 to 6 GΩ) were filled with ion-selective ionophore cocktails (Sigma, Buchs, Switzerland) and their tips were moved towards and away from the sample in a slow (5 s cycle ...
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bioRxiv - Biochemistry 2021Quote: ... The culture supernatant containing the target protein was concentrated 3-fold and buffer exchanged against 6 volumes of working buffer (50 mM Tris/HCl, 300 mM NaCl, pH 8.0) by crossflow filtration (Millipore Pellicon 2 mini filter ...
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bioRxiv - Immunology 2020Quote: 1×107 draining lymph node or spleen cells were restimulated for 6 hours with 10μM MCC peptide with the addition of 5μM Brefeldin A (Sigma Aldrich) for the final 4 hours ...
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bioRxiv - Neuroscience 2020Quote: ... The oxygen electrodes were calibrated in air-saturated 0.9% sodium chloride (at 37°C) and oxygen-free standard solution [0.1M sodium hydroxide (SX0607H-6, Sigma-Aldrich) and 0.1M sodium ascorbate (A7631 ...
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bioRxiv - Biochemistry 2021Quote: ... EMSA reactions were then run on a non-denaturing 0.5X TBE 6% polyacrylamide gel and stained with ethidium bromide (Sigma). Band intensities were quantified using ImageJ.
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bioRxiv - Cell Biology 2021Quote: ... the cells were split into 6-well plates at a density of 0.5×106 cells/well and subjected to 2µg/mL puromycin (Sigma P8833) for selection of infected cells ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted using 1.6 ml of extraction/washing buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton, 0.2% NP-40, 6 mM BME and 1x protease inhibitor cocktail [SIGMA, USA]) during 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were resolved on 4-12% Bis-Tris Gels (Thermo) or homemade 6% Bis-Tris gels and transferred to 0.45 μm PVDF (Millipore) membranes ...