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Citations for Millipore Sigma :
8151 - 8200 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Recombination of the conditional allele was induced in Atxn10flox/flox;CAGG-creERT2 mice at 6 and 8 weeks old by a single intraperitoneal (IP) injection of 9mg tamoxifen (Millipore Sigma, T5648) per 40g (body weight ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22, CRP; Millipore Sigma - RAB0096-1KT). In brief ...
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Dopamine depletion can be predicted by the aperiodic component of subthalamic local field potentialsbioRxiv - Neuroscience 2021Quote: ... 4 or 8 μl of 6-OHDA (5 μg in 1 μl saline containing 0.1% ascorbic acid; Tocris, Bristol, ENG and Sigma-Aldrich, MA, USA) was injected into the left MFB at the following coordinates ...
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bioRxiv - Molecular Biology 2021Quote: ... A BioSpec bead-beater was used to lyse cell pellets corresponding to 3-6 OD600 units in the presence of 100 μL of acid washed beads and 300 μL of 20 % TCA (Sigma-Aldrich T6399). 2 x 3-minute pulses were used ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 x105 HEK293 cells were seeded in each well of a 6-well plate and were transfected with 2 μg of respective plasmid DNA using PEI reagent (Sigma-Aldrich, 408727). After 24 hours ...
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bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
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bioRxiv - Immunology 2019Quote: ... erythrocytes were depleted by sequentially layering 10 ml PBS followed by 8 ml of 6% w/v dextran (high molecular weight, Sigma-Aldrich, in PBS) and gently inverting ...
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bioRxiv - Genomics 2019Quote: ... and 12.5 ng/µL oligo(dT) at 42°C for 2 h in the presence of 6 ng/µL actinomycin D (Sigma, #A1410-2MG) to prevent antisense artifacts (Perocchi et al ...
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bioRxiv - Bioengineering 2020Quote: ... and 800 mg L-1 humic acids (CAS No. 1415-93-6) were prepared by adding in ultrapure water (Sigma Aldrich, UK).
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bioRxiv - Neuroscience 2020Quote: ... and MT+SNL mice from tissues collected on day 6 (post behavioral testing) using Tri Reagent (T9424, Sigma Aldrich, St. Louis, MO) and RNeasy Micro kit (74106 ...
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bioRxiv - Neuroscience 2021Quote: ... iPSC-derived neuron progenitors were expanded using 6-well plates coated with 15 μg/ml Poly-L-Ornithine (Millipore Sigma Cat# P4957) and 10 μg/ml laminin (Invitrogen Cat# 230171015 ...
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bioRxiv - Cell Biology 2021Quote: ... lentiviral particles were generated by transfecting HEK293T cells with 6 μg PLKO.1 scramble control and MISP-targeted shRNA plasmids (SIGMA; TRCN0000422523, TRCN0000116527), 4 μg psPAX2 packing plasmid (Addgene ...
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bioRxiv - Neuroscience 2020Quote: Primary hippocampal neurons were isolated from P1 C57BL/6 mice by dissociating with 0.25% trypsin and plated onto poly-L-lysine (Sigma-Aldrich, MO, USA)-coated culture dish ...
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bioRxiv - Neuroscience 2020Quote: ... The heads were placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2700, Sigma-Aldrich, U.S.A.). Data was acquired on a small animal MRI system (BioSpec 94/30 animal MRI system ...
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bioRxiv - Immunology 2021Quote: ... Fab samples were concentrated to ~6-8 mg/ml and buffer exchanged to TBS using Amicon ultrafiltration units with a 10 kDa cutoff (EMD Millipore Sigma). Final Fab yields were ~1 mg ...
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bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
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bioRxiv - Neuroscience 2020Quote: Focal white matter demyelinating lesions were induced in the cerebral striatum of 12-week-old male C57BL/6 mice by stereotaxic injection of 4 μL of 0.01% (v/v) ethidium bromide (Sigma-Aldrich, Shanghai, China) using a Hamilton syringe ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were collected 6 days after plating in 1 ml PBS and incubated with 60-80 ul collagenase IV [10mg/ml] (Sigma #C5138-1G) at 37 C for 10 mins ...
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bioRxiv - Cancer Biology 2022Quote: HEK293T cells at 80-90% confluency in a 12-well plate were transfected using 6 μL GeneJuice Transfection Reagent (Sigma-Aldrich, 70967) and 100 μL RPMI 1640 medium (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... cells were fixed using a 6% (v/v) formaldehyde: phosphate-buffered saline solution and stained with 1% (w/v) crystal violet (Sigma-Aldrich, Italy) in 70% (v/v ...
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bioRxiv - Immunology 2020Quote: ... single cell suspensions were derived from bone marrows of 4-6-week-old C57BL/6 WT and Igh VH locus inversion mice and incubated in Red Blood Cell Lysing Buffer (Sigma-Aldrich, #R7757) to deplete the erythrocytes ...
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bioRxiv - Biochemistry 2021Quote: ... Female C57BL/6 mice wild type mice (7-9 weeks) were injected intraperitoneally with etomoxir (100 µg, Sigma Aldrich, Cat. No E1905) and/or LPS (1 µg ...
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bioRxiv - Molecular Biology 2020Quote: Confluent monolayers of HAEC grown on 6-well plate were exposed to unidirectional PSS (6, 12 and 24 hours) using a modified flow device.10 Neutralized MCDB-131 medium (Sigma Aldrich, MO) containing 7.5% sodium bicarbonate solution ...
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bioRxiv - Neuroscience 2021Quote: ... 6; MgCl2, 4; CaCl2, 5; sucrose, 160; glucose, 25; Hepes, 10; pH 6.7, 500 mOsmol; all chemicals from Sigma-Aldrich, Lyon, France), to avoid desiccation of the brain surface ...
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bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2022Quote: ... mice aged 5 – 6 weeks and harboring the Fezf2-CreER allele were treated with 50 mg/kg body weight tamoxifen (Sigma-Aldrich,T5648) by oral gavage for 5 consecutive days ...
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bioRxiv - Cell Biology 2022Quote: ... cells were seeded in 6-well plates the previous day and treated either with 200ug/ml Cycloheximide (CHX) (Sigma-Aldrich # C-7698) in fresh growth media for 2 ...
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 media containing 10% FBS was supplemented with antibiotics (6 mg/l Nitrofurantoin, Sigma Aldrich, cat. no. N7878; 16 mg/l Imipenem monohydrate, Sigma Aldrich, cat ...
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bioRxiv - Biophysics 2022Quote: The dyes used in this study and the working dilutions were: 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine (LAURDAN, 4.5 μM, Sigma-Aldrich, Merck, #40227), Hoechst 33342 (80 uM ...
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bioRxiv - Immunology 2019Quote: ... duplicate wells of a 12-well plate were seeded with 1 ml/well of the cell suspension (1 × 106 cells/ml) and virus was added in the presence of 6 μg/ml polybrene (Sigma-Aldrich, #107689) (multiplicity of infection of 25) ...
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bioRxiv - Zoology 2019Quote: ... or cortisol treatments (8 g peanut butter, 2 g wheat germ with 6, 8, or 12 mg of cortisol [H4001, Sigma Aldrich, USA]). Dosages of 0 ...
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bioRxiv - Zoology 2019Quote: The midgut tissue dissected out from continuous toxin-exposed susceptible and toxin exposed insects for 6 generations (n=5) were processed for RNA isolation using TRI reagent TM (Sigma Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... 107 cells cultured with 100nM 4-OHT or ethanol control for 6 or 24 hours were incubated with 1% formaldehyde (Sigma-Aldrich, F8775) at room temperature for 10 min ...
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bioRxiv - Plant Biology 2019Quote: ... Then ten half seeds with involved embryos were placed in a Petri dish containing 6 ml of distilled water with or without (±) 10 μM of ABA (Sigma Chemical Co.). The Petri dishes were then incubated in the dark at 20°C ...
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bioRxiv - Pathology 2021Quote: ... Infected tissues were disrupted and digested overnight (12 hours) using Fast Prep® Lysing Matrix tubes containing 0.2 mg (6 U) Proteinase K (Sigma-Aldrich®) diluted 1/40 in Lifton’s Buffer (100 mM EDTA ...
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bioRxiv - Neuroscience 2019Quote: ... filled with a thin layer of 2.5% agarose containing either pure agarose (Sigma Aldrich, cat. no.: A5093, CAS no.: 9012-36-6) or agarose plus 1.5 M sodium chloride (Sigma Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Th-1 and Th-17 cells were restimulated for 6 hr in the presence of PMA (100 ng/ml, Sigma, Cat#P8139), Ionomycin (1 μM ...
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bioRxiv - Neuroscience 2019Quote: ... The heads were then placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2700, Sigma-Aldrich, U.S.A.). Samples were measured at room temperature ...
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bioRxiv - Immunology 2019Quote: ... CHO cells pre-incubated with trivalent Strep-Tactin-SpyCatcher or buffer alone were incubated with 2 µM ATTO 647 biotin (ATTO Technology # AD 647-71) pre-mixed with 6 µM biotin (Sigma-Aldrich # B4501) for 30 minutes at 25 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... isolated of at least 6 animals per preparation were mixed with collagen type I (PureCol™ EZ Gel solution, # 5074, Sigma-Aldrich, Vienna ...
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bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... After rinsing three times for 10 minutes in TBS at RT sections were incubated with 4?,6-Diamidin-2-phenylindol (Dapi, Sigma, Cat# D9542) for 10 minutes at RT and washed once for 10 minutes in TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Y1eGFP mice at 6-8 weeks of age were transcardially perfused with chilled phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (Sigma-Aldrich, USA) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded in 6-well plates and 24 h later CIGB-300-F and Hoechst 33342 (Sigma, St. Louis, MO, USA) were added at a final concentration of 30 μM and 4 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... Lentiviral-containing supernatant was collected at 72 hours and used to transduce SAF-A-AID-mCherry cells in the presence of RPMI media supplemented with 10% FBS and 6 μg/mL polybrene (Sigma, H-9268). After 48 hours ...
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bioRxiv - Developmental Biology 2021Quote: The proteins in the sample were precipitated using methanol/chloroform and were resuspended in 6 M Guanidine HCl (Sigma cat# G3272-1KG), buffered using 50 mM EPPS (Alfa Aesar cat# A13714) ...
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bioRxiv - Biophysics 2021Quote: ... 3C cleavage was performed in 30 mins and the protease was separated from HsPepT2 by gel filtration using a Superose® 6 Increase 10/300 (Sigma-Aldrich). The top fraction was concentrated to 10 mg/mL using a 100 kDa cutoff concentrator (Corning Spin-X UF concentrators ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and urethane (ethyl carbamate, EC; CAS# 51-79-6) from Sigma (St. Louis, MO). Primer sets were Kras ...