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Citations for Millipore Sigma :
8001 - 8050 of 10000+ citations for UDP β L Arabinofuranose since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein expression was induced with 1 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma; 16°C, 200 RPM, 20 hours). Cells were harvested by centrifugation (5000 RPM ...
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bioRxiv - Neuroscience 2023Quote: ... (AGP-001 and ACC-003, Alomone), blocking peptide for Anti-CaV1.2 (CACNA1C) (BLP-CC003, Alomone) and β-ACTIN (A1978, Sigma-Aldrich). Secondary antibodies used for the immunoblots were Mouse-HRP (115-035-003 ...
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bioRxiv - Cell Biology 2023Quote: ... Receptor-mediated endocytosis was monitored in the proximal tubule of mouse kidneys PTs by measuring the uptake of β-lactoglobulin (L3908, Sigma). Briefly ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... and analyzed by western blot with anti-SARS-CoV-2 N protein monoclonal antibody (0.5 μg/mL, anti-SARS-CoV-2 N mAb, clone 1G10C4 mAb)42 and anti-β-actin antibody (Sigma-Aldrich, cat ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of the recombinant proteins was induced by 1 mM isopropyl β-D-thiogalactopyranoside at 37 °;C for 5 h in host Escherichia coli strain Rosetta (DE3) pLysS cells (Novagen). After induction ...
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bioRxiv - Neuroscience 2023Quote: ... were treated to prevent proliferation with cytosine β-D-arabinofuranoside (ara-c; C1768, 2.5 µM, Sigma-Aldrich, St. Louis, MO, USA) and frozen in liquid nitrogen until plated in co-culture with neurons ...
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Single-cell transcription mapping of murine and human mammary organoids responses to female hormonesbioRxiv - Developmental Biology 2023Quote: ... Organoid conditions with “low” levels of estrogen were grown with media supplemented with 33.3 ng/mL of 17-β-Estradiol (Sigma #E2758), and those with “high” levels of estrogen were grown in the presence of 66.6 ng/mL of 17-β-Estradiol ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were diluted in 1X Laemmli sample buffer and heated at 95°C for 10 min in the presence of β-mercaptoethanol (Sigma) (except for OXPHOS cocktail antibody according to manufacturer’s instructions) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell suspensions were mixed with 350 µl of freshly prepared lysis buffer (2M NaOH, 7% β-mercaptoethanol) and 350µl of 50% TCA (Sigma, T9159). After spin ...
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bioRxiv - Physiology 2024Quote: ... were spread onto NGM plates containing 100 μg/ml ampicillin and 1 mM isopropyl 1-thio-β-Dgalactopyranoside (IPTG, 420322, Millipore) for 24 hrs ...
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bioRxiv - Bioengineering 2023Quote: To generate emulsions, 1 mL of fluorinated oil (Novec 7500, ACOTA) and 2 mL of protein solution (β-lactoglobulin; >90%, Sigma, from bovine milk ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit poly-HRP secondary antibody (Thermo, 32260) and 1:10,000 anti-β-actin-peroxidase antibody (Sigma, A3854) in 5% bovine serum album/TBS-T for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated with primary antibodies (anti-PYK2 #3292; Cell Signaling Technology, anti-FAK 610088; BD Biosciences, anti-β-actin A5441; Sigma-Aldrich) overnight at 4°C and then with secondary antibodies (LiCOR ...
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bioRxiv - Microbiology 2023Quote: ... The final dilution of the primary antibody was 1:2,000 for anti-HA tag (Covance) and 1:3000 for anti-β-actin (Millipore-Sigma).and K-Rta (65).
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bioRxiv - Cancer Biology 2023Quote: ... Protein loading was determined using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000) from Sigma-Aldrich (A3854). Quantitation of western blot band intensity was done using Image J software.
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bioRxiv - Cancer Biology 2023Quote: ... staining solution (0.3 mg/ml of 5-bromo4-chloro-3indolyl β-D-galactoside, X-Gal Fermentas R0401, 40 mM citric acid, Sigma C-0759, 40 mM sodium phosphate, Sigma S5011 ...
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bioRxiv - Cell Biology 2023Quote: ... in PBST (1x PBS, 0.1% Tween20), incubated in β-tubulin (1:1000, CST 2146S) or actin C4 (1:1000, Sigma MAB1501) diluted in 1% milk with 1% BSA in PBST overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... Rabbit α-mouse Calmodulin (5μg mL-1, CellSignaling), rabbit α-mouse β-actin (1μg mL-1, CellSignaling) and mouse α-mouse GAPDH (1μg mL-1, Merck/Millipore), goat-α-mouse IRDye 680RD ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were incubated for 2 hours at room temperature with monoclonal anti-β-actin HRP-conjugated mouse antibody (A3854, Sigma-Aldrich). Membranes were developed with Immobilon® Forte Western HRP Substrate (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... islets isolated from chow-fed C57BL/6 WT mice were pre-incubated in 20 mM cholesterol loaded onto methyl-β-cyclodextrin (MβCD) (C4951, Sigma Aldrich) diluted in 1X Krebs-Ringer bicarbonate-Hepes (KRBH ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was blocked with 1% BSA with 0.05% Tween-20 and reacted with an anti-tau antibody (RTM38, 1:2500) and anti-β-actin antibody (1:10,000; Sigma-Aldrich) at RT for 1 h (or 4°C O/N) ...
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bioRxiv - Biochemistry 2024Quote: Reaction mixes were prepared with the desired ⍺β-tubulin concentrations (7 μM or 10 μM) containing 5% Alexa 568-tubulin plus 1 mg/ml BSA (Sigma) in assay buffer (BRB80 with 30 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (EMD Millipore Corp.; Cas: 367-93-1). Cells were then incubated for 18 to 20 hours at 20°C at 200 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... β-galactosidase (X-gal) lung staining was performed on frozen lung sections using the Senescence Cells Histochemical Staining Kit (Sigma CS0030) following manufacturer instructions ...
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bioRxiv - Genetics 2024Quote: ... Laemmli Buffer (100 mM Tris pH 6.8, 20% glycerol, and 4% SDS (Carl Roth, Germany)) and 5% β-mercaptoethanol (Sigma-Aldrich) were added to the samples after which they were heated to 95°C for 10 minutes.
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bioRxiv - Plant Biology 2020Quote: ... seeds were plated on the medium described above containing 220 mg/L lincomycin (Sigma Aldrich).
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bioRxiv - Plant Biology 2019Quote: ... water was replaced with assay buffer containing 34 mg/L luminol sodium salt (Sigma, #A4685), 20 mg/L horse radish peroxidase (Fujifilm Wako ...
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bioRxiv - Biophysics 2021Quote: ... incubated for another 15 min with 1 mg/mL poly-L-lysine (PLL; Sigma P8920), and rinsed with actin polymerization buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-treated with a 0.01% Poly-L-Lysine solution (Sigma Aldrich® A-005-M) to facilitate cell adhesion ...
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bioRxiv - Biophysics 2019Quote: ... or poly-L-lysine and laminin (PLL, P2636; LN, L2020, Sigma-Aldrich, St. Louis, USA). Hydrogels were first dehydrated until the surface looks dry ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 10 μM 6-Diazo-5-oxo-L-norleucine (DON, Sigma Aldrich), 1 μM Palbociclib (Sigma Aldrich ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Cells were plated onto the 96-well plates coated with poly-L-ornithine (Sigma-Aldrich). Equal volumes of cell suspension were plated onto the center of the dish ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl) and eluted with 300 ng/L of FLAG peptide (#F4799, Sigma-Aldrich) for four hours under moderate agitation 62 ...
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bioRxiv - Molecular Biology 2021Quote: ... Prior to cell seeding the plates were treated with 0.01% Poly-L-Ornithine (Millipore, A004C) for at least 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Nitric oxide synthase (NOS) inhibition was achieved by incubation with 0.5mM L-NAME (Sigma-Aldrich) diluted in E3 medium.
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bioRxiv - Evolutionary Biology 2020Quote: ... Each fish was anesthetized with MS-222 (Sigma-Aldrich, 0.07 g/l of seawater; [19]), recognized through microchip ID reading ...
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bioRxiv - Bioengineering 2022Quote: ... coloured for 3 h with 0.1 mg/L Evans Blue in PBS (#E2129, Sigma Aldrich), extensively washed in PBS and finally nuclei stained with 3 nM DAPI is PBS ...
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bioRxiv - Neuroscience 2021Quote: ... plates or dishes were coated with 10 µg/ml Poly-L-Ornithine (PLO, Sigma, P4957) in PBS for at least 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... and the cysteine residues were carbamidomethylated with iodoacetamide (final concentration 15 mmol/L; Sigma-Aldrich), after which a pooled quality control (QC ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured intact mouse or human islets were resuspended in 0.1mmol/L EGTA (Sigma-Adlrich, 234626) in serum free CRMLfollowed by transduction of indicated adenoviral constructs at 10,000 plaque-forming unit per islet equivalent (pfu/IEQ ...
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bioRxiv - Cell Biology 2022Quote: Zebrafish were sacrificed by lethal dose of 1g/L of MS-222 (Sigma, MO, USA) and each tissue (gonads ...
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bioRxiv - Cell Biology 2022Quote: Zebrafish were sacrificed by lethal dose of 1g/L of MS-222 (Sigma, MO, USA), fixed for 72 hr in 10% neutral buffered formalin and decalcified in 0.5M EDTA for 48 hr at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... 50µg/ml 2-Phospho-L-ascorbic acid trisodium salt and 100 nM dexamethasone (Sigma-Aldrich), adapted from Ghali et al29 ...
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bioRxiv - Bioengineering 2019Quote: ... 0.25 g/L of magnesium sulfate heptahydrate (MgSO4 ·7H20) (Sigma-Aldrich, cat no. 230391-500). The following solutions were added to DSM after autoclaving ...
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bioRxiv - Physiology 2019Quote: ... Plasma amino acid levels were determined using the L-Amino Acid Quantitation Kit (Sigma Aldrich) following manufacture’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM L-glutamine and 50 unit/ml penicillin/streptomycin (Sigma-Aldrich Chemie, Munich, Germany). In some cases ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were counted and 15,000 cells were plated on each poly-l-lysine coated (Sigma) glass coverslip (Carolina Biological ...
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bioRxiv - Neuroscience 2019Quote: ... and plated onto 6 well cell culture dishes coated with Poly-L-Ornithine (Sigma P2533) at a density of 1 million cells per well.
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bioRxiv - Biochemistry 2020Quote: ... S2 cells were allowed to settle on coverslips treated with Poly-L-Lysine (Sigma-Aldrich). After gentle washing ...