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Citations for Millipore Sigma :
7951 - 8000 of 10000+ citations for UDP β L Arabinofuranose since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... at the Center for Regenerative Medicine (CRM) Flow Cytometry Core Facility (MGH, Boston, MA) in β-estradiol (0.5 μM, Sigma Aldrich) containing media ...
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bioRxiv - Immunology 2019Quote: ... Cells were plated at 0.5-1 × 105 cells in a 96 well plate in L-15 media containing 5% FCS and exposed to DMSO (vehicle control) or 50 ug/ml 17-β estradiol or Bis-phenol A (Sigma) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse antibodies used in the study were raised against β-actin (Sigma-Aldrich, Clone AC-15, # A1978, 1:1000 for WB), GFP (Abcam ...
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bioRxiv - Neuroscience 2019Quote: Mice were treated daily by oral gavage with 0.5 mg/kg FTY-720 (Selleckhem) in 2% (2-hydoxypropyl)-β-cyclodextrin (Sigma-Aldrich) starting on day four post-immunization ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared for Western Blot by diluting 8μg protein 1:4 with loading buffer (1:10 β-mercaptoethanol (Sigma-Aldrich) and 9:10 laemmli (Bio-Rad)) ...
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bioRxiv - Cell Biology 2021Quote: β-Estradiol induction of the pRPS5A::XVE:GFP-μHD line was done by transferring 3-day-old seedlings to medium containing β-Estradiol (Sigma-Aldrich) or solvent (DMSO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with a mixture of protease and phosphatase inhibitors (1 mM PMSF, 10 mM NaF, 0.3 mg/mL CalyculinA, 1 mM sodium orthovanadate, 10 mM β-glycerol-phosphate; all from Sigma-Aldrich and complete protease inhibitor cocktail ...
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bioRxiv - Molecular Biology 2021Quote: ... Adherent bone marrow stromal cells were then re-plated in triplicate in 12 well plates at 2×105 cells per well with ascorbic acid and 10 mM β‐glycerophosphate (Sigma) to perform qPCR assays ...
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bioRxiv - Biochemistry 2021Quote: All-trans-astaxanthin and β-carotene (CAS Numbers: 472-61-7 and 7235-40-7) were purchased from Sigma-Aldrich (USA). Liposomes were prepared according to the Racker method [72] with modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then blocked with a 5% milk in TBST solution and then probed with primary antibodies directed against β-actin (Sigma), vinculin (Abcam) ...
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bioRxiv - Physiology 2021Quote: ... each sample was homogenized by pestle using 350μl of RLT buffer (supplemented with β–mercaptoethanol from Sigma-Aldrich instead of DTT) on ice and passed on a QIAshredder column (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were blocked in blocking buffer (5% skim milk, 0.1% Tween in PBS) and incubated with β-Actin (#A5316, Sigma-Aldrich), MYC (#9402 ...
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bioRxiv - Immunology 2020Quote: ... were stimulated with IFN-β for 24 hours prior to harvest by trypsinization and lysis in RIPA buffer supplemented with protease inhibitor cocktail (Sigma) and phosphatase inhibitor cocktail II (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... the freshly prepared Au-coated glass was immersed into a linker solution containing β-mercaptoethanol (βME, Sigma-Aldrich, St. Louis, MO), PEG-SH (#MPEG-SH-2000 ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 transformed with recombinant pGEX-2T plasmids were diluted 1:10 with fresh medium and grown for 1 h at 37°C before inducing with 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG, Sigma). After another 4 h incubation at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Genomics 2021Quote: ... 6 ml of ER-HoxB8 cell culture media (RPMI 1640 supplemented with 10% FBS, 1% penicillin/streptomycin, 0.5 μM β-estradiol (Sigma-Aldrich), and 20 ng/ml GM-CSF (PeproTech) ...
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bioRxiv - Genomics 2021Quote: ... were selected and mono-nucleosomal DNA fragments were purified from 3% low melting-temperature agarose gels and treated with β-agarase (Sigma) for sequencing.
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bioRxiv - Microbiology 2020Quote: ... The temperature was subsequently reduced to 18°C and protein production was induced with 0.5 mM Isopropyl-β-D-thiogalactoside (IPTG, Sigma Aldrich) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... The primary antibodies against β-Actin and P53 were directly conjugated to HRP and were used as follows: mouse monoclonal anti-β-Actin-HRP at a 1:50,000 dilution (A3854 from Millipore Sigma); and goat polyclonal anti-p53-HRP at a 1:3,000 dilution (HAF1355 from R&D systems) ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were digested with 10µl of 20T 50mg/ml zymolyase (Nacali Tesque, 07663-91) and 15µl β-mercaptoethanol (Sigma-Aldrich, M3148) for 45 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA; Cat#E3889, Sigma-Aldrich, St Louis, MO, USA) 1 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... cell pellets were lysed in 8M urea buffer supplemented with phosphatase inhibitors (10 mM Na3VO4, 100 mM β-glycerol phosphate and 25 mM Na2H2P2O7 (Sigma)) ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Immunology 2022Quote: Cells were lysed by addition of a mild lysis buffer containing 1% octyl-β,D-glucopyranoside (#O9882-500MG, Sigma-Aldrich, Merck), 0.25% sodium deoxycholate (#1065040250 ...
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bioRxiv - Plant Biology 2022Quote: ... 200 μl of supernatant was transferred to a new tube and incubated with 20 μl of 0.5 U/μl β-glucosidase (Sigma-Aldrich) for 90 min at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Two weeks after growing in liquid medium effector expression was induced with 100 μM β-Estradiol (cat. no: E8875-1G; Sigma) for 24 h ...
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bioRxiv - Plant Biology 2022Quote: ... NEROSs-RFP-Flag and GFP were transiently expressed in 4-week-old tobacco (N. benthamiana) leaves and the expression was induced using 100 μM β-Estradiol (cat. no: E8875-1G; Sigma). Leaves were dark-adapted for 20 min before chlorophyll fluorescence and ETR were measured at 20°C ...
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Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... of liver homogenate (0.08 M NaPO4) was mixed with 30 μL of p-nitrophenyl-2-acetamide-β-D-glucopyranoside (Sigma-Aldrich) and diluted in 50 μL of 50 mM citrate buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells were grown at 37 °C and were later subjected to Isopropyl β-d-1-thiogalactopyranoside (IPTG, Sigma-Aldrich, USA) induction at an OD600 ∼ 0.6-0.8 with 0.7 mM at 37 °C for 6 hours for PfPHB1-pGEX-4T 1 and 0.7 mM at 16 °C for 20 hours for PfPHB2-pGEX-4T 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Eventually, the blots were probed with indicated primary antibodies: anti-HA (261883, Thermo Fischer) and anti-β-actin (A2228, Sigma-Aldrich) antibodies overnight at 4°C followed by incubation with secondary anti-rabbit (711-035-152 ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were subjected to the following primary antibodies overnight at 4°C according to the experiment: mouse monoclonal anti-β-tubulin III (1:5000; Sigma) or mouse monoclonal anti-actin (1:4000 ...
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bioRxiv - Molecular Biology 2022Quote: ... β-actin levels were used as an internal control in each sample (A3854, 1:20,000 dilution, Sigma-Aldrich, St. Louis, MO). Protein bands were quantified with ImageJ and Image lab (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: Rats received either a 30-minute infusion of corticosterone (0.75 mg/mL Corticosterone-2-hydroxypropyl-β-cyclodextrin; Sigma-Aldrich, Gillingham, UK) dissolved in sterile 0.45% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... half of the medium was substituted with a culture medium supplemented with 2% B-27 plus supplement and 5μM of cytosine β-d-arabinofuranoside (Ara-C; Sigma; #C1768). The culture medium was then changed every three days ...
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bioRxiv - Physiology 2024Quote: ... were spread onto NGM plates containing 100 μg/ml ampicillin and 1 mM isopropyl 1-thio-β-Dgalactopyranoside (IPTG, 420322, Millipore) for 24 hrs ...
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bioRxiv - Molecular Biology 2024Quote: ... and MEFs whole cell extracts were prepared by lysing cells in 4xLDS buffer (-EDTA) containing β-mercaptoethanol and 500 U Benzonase (Millipore) at 10,000 cells / µL for 10-15 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... after which it was incubated with 12.5 mM 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (total HEX; Sigma Aldrich, MS, USA) or 5 mM 4-methylumbelliferyl N-acetyl-β- D-glucosaminide-6-sulfate (HEXA ...
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bioRxiv - Cancer Biology 2023Quote: ... staining solution (0.3 mg/ml of 5-bromo4-chloro-3indolyl β-D-galactoside, X-Gal Fermentas R0401, 40 mM citric acid, Sigma C-0759, 40 mM sodium phosphate, Sigma S5011 ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were disrupted and homogenized using the NG010 Tissue Grinder Mixy Professional (NIPPON Genetics) in 600 µl of RLT Buffer containing β-mercaptoethanol (M625, Sigma). Extracted RNA was stored at −80 until processed for sequencing.
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bioRxiv - Biochemistry 2024Quote: ... the induction was done either by 1% (w/v) lactose or 0.5 mM isopropyl-β-D-1-thio-galactopyranoside (IPTG, Sigma Aldrich) (Table 1) ...
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bioRxiv - Biochemistry 2024Quote: ... β-Nicotinamide adenine dinucleotide phosphate disodium salt (NADP+) and tris(2-carboxyethyl)phosphine) (TCEP) was purchased from Millipore Sigma (Burlington, MA). Nickel-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Cell Biology 2023Quote: ... in PBST (1x PBS, 0.1% Tween20), incubated in β-tubulin (1:1000, CST 2146S) or actin C4 (1:1000, Sigma MAB1501) diluted in 1% milk with 1% BSA in PBST overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... before counting in complete RMPI (cRPMI = naRPMI supplemented with 10% heat-inactivated FCS [#S-FBS-EU-015, Serana, Pessin, Germany], 25 nM β-mercaptoethanol [#M6250, Sigma] ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in LB media and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma Aldrich, I1284). Bacteria pellets were resuspended in Bugbuster (Sigma Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... followed by blocking with 5% non-fat milk and incubation with primary antibody against Cas9 (CST, #14697, 1:2,000), YAP1 (CST, #14074, 1:2,000), MTAP (CST, #4158, 1:2,000) or β-actin (Sigma, #A5316, 1:10,000). After overnight incubation at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% bovine serum albumin and probed with the following primary antibodies: β-actin (Sigma, Cat. No. A1978), EGFR (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription was induced by replacing the media with RPMI media supplemented with charcoal stripped FBS and 100nM β- Estradiol (Sigma-Aldrich) (pre-dissolved in ethanol ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets from control and subject fibroblasts and corresponding overexpression cells were solubilized in 1% n-dodecyl-β-D-maltoside (DDM, Sigma) in PBS with 1x cOmplete™ protease inhibitor cocktail (Roche Diagnostics ...
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bioRxiv - Microbiology 2023Quote: ... 1% Triton X-100,140 mM NaCl and 0.1% SDS] supplemented with 50 μl β-mercaptoethanol and 1 mM PMSF (Sigma-Aldrich, USA) per ml of RIPA buffer ...