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Citations for Millipore Sigma :
8001 - 8050 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... After the overnight growth at 37 °C, the cells were harvested by centrifugation (30 min, 4300 g) and plasmid purified by midi-prep (Millipore Sigma).
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bioRxiv - Developmental Biology 2024Quote: ... cells were transfected with 2 µg of pCS2+empty or pCS2+HySp5 plasmid using the X-tremeGENE HP DNA transfection reagent (Sigma, 6366546001). To prepare cell extracts 24 hours later ...
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bioRxiv - Cell Biology 2024Quote: HeLa Kyoto cells transiently expressing the indicated plasmids were grown in a 10-cm dish with or without starvation treatment using Earle’s Balanced Salt Solution (Sigma-Aldrich, E2888). Proteins bound to the beads were reduced with 10 mM TCEP and alkylated with 20 mM IAA ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid-encoded wild-type and mutant rRNA overexpression (Figure 2B) was induced by adding 12 mM sodium propionate (Sigma-Aldrich #P1880) to bacterial cultures at an OD600 of ∼0.2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The strain was transformed with a plasmid containing a 3-methyl benzoate (3MB; m-Toluic Acid; Sigma Aldrich Catalog No. T36609) inducible recT recombinase and mutLE36K (pTE583 ...
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bioRxiv - Microbiology 2024Quote: ... cells in each culture plate were transfected with 10µg of pNL4-3 plasmid DNA complexed with 50μL PEI reagent (Sigma-Aldrich catalog # 764604), and the culture media was changed 16 hours post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the primary antibody (monoclonal mouse anti-vinculin, 1:100, Sigma V9264 ...
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bioRxiv - Cancer Biology 2021Quote: ... histone H3K27me3 (rabbit, mAb, clone C36B11, CST) histone H3 (mouse, mAb, clone 6.6.2, Millipore Sigma), RNA Polymerase 2 (rabbit polyclonal ...
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bioRxiv - Biophysics 2019Quote: ... antibodies were used in combination with peroxidase-labeled goat anti-mouse IgG (A4416, Sigma-Aldrich). The reactivity was detected with an Amer-sham ECL Detection Reagent (GE Healthcare Life Science ...
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bioRxiv - Microbiology 2021Quote: ... IL-22) were determined using the Mouse Th17 Magnetic Bead Panel Milliplex MAP kit (Millipore). Samples were not diluted and assay was performed according to the manufacturer’ instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary Abs were anti-mouse/rabbit IgG peroxidase antibody produced in goat (A0168/A6667; Sigma); Alexa Fluor 488 goat anti-mouse/rabbit IgG (A11029/A11034 ...
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bioRxiv - Cell Biology 2020Quote: We used three different antibodies against γ-H2AX: a mouse monoclonal antibody (Millipore, 05-636) at a 1:500 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibodies used were: mouse anti-γ-tubulin (1µg/ml, Sigma-Aldrich GTU-88), mouse DM1α (1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... and treated with FITC anti-mouse IgG antibody (F0257, Sigma; 1:1000 in blocking buffer) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and treated with FITC goat anti-mouse antibody (F0257, Sigma; 1:2000 in blocking buffer) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... followed by horseradish peroxidase-conjugated goat anti-mouse or goat anti-rabbit IgG (Sigma-Aldrich) and Amersham ECL Select Western Blotting Detection Reagent or Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Alpha tubulin was detected by the monoclonal mouse anti-alpha tubulin antibody (1:20’000, Sigma) and the rabbit anti-mouse-IgG conjugated to horseradish peroxidase (1:10’000 ...
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Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosisbioRxiv - Cell Biology 2020Quote: ... Primary antibodies were used at the following concentrations: anti-GFP (mouse, 1:1000; Sigma F1804), anti-SYP-1 (goat ...
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bioRxiv - Cell Biology 2020Quote: The primary antibodies used in this study are mouse anti-Hsp90B1 (1:300; Sigma, AMAb91019), rabbit anti-EGFP (1:300 ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal anti-HA antibody-conjugated agarose beads (mouse monoclonal anti-HA-agarose antibody) (Sigma #A2095) were added to the cleared extracts and incubated at 4°C for 4 h ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used were mouse monoclonal anti-FLAG® (Sigma-Aldrich, clone M2; 1:1,000 dilution), anti-Pyrin (Adipogen ...
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bioRxiv - Immunology 2021Quote: ... the lysate supernatant was precleared with 10 µl/dish mouse IgG- Agarose beads (Sigma A0919) for a minimum of 30min at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies used included mouse anti-FLAG (1:100-1:2,000 dilution; Sigma clone M2), rat anti-HA (1:2000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... or β-Actin were detected using mouse monoclonal primary antibodies against FLAG (M2, F1804, Sigma), VSV M (Kerafast ...
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bioRxiv - Microbiology 2019Quote: ... Then membranes were incubated with HRP-conjugated secondary anti-mouse/rabbit antibodies (1:10,000; Sigma). Blots were developed with ECL (Pierce).
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bioRxiv - Immunology 2021Quote: ... The mouse anti-fibrillin-1 mAb (catalog number MAB2502) was purchased from Millipore (Temecula, CA). The secondary antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... PCNA (rabbit polyclonal, Abcam, ab2426, RRID:AB_303062, 1:300; mouse monoclonal, Millipore, CBL407, RRID:AB_93501, 1:300), Sox2 (goat polyclonal ...
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bioRxiv - Plant Biology 2022Quote: ... followed by anti-mouse Ig-HRP antibody (1:5000 dilution; Sigma-Aldrich, cat. no. A9044) and blots were incubated with SuperSignal Femto substrate (Thermo ScientificTM ...
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bioRxiv - Neuroscience 2022Quote: ... horizontal sections were then incubated with primary antibody mouse anti TH 1:1000 (Sigma, T1299) and rabbit red fluorescent protein (RFP ...
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bioRxiv - Neuroscience 2020Quote: ... the following were used as primary antibodies: mouse anti-GFP (1:100, Sigma-Aldrich, G6539), rat anti-DN-cadherin (1:25 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with an anti-GFAP mouse monoclonal antibody (1:1000, Sigma-Aldrich G3893) for 48 h at 4°C on a shaker ...
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bioRxiv - Molecular Biology 2021Quote: We employed the following antibodies for immunofluorescence: mouse anti-γH2AX (clone JBW301, Millipore, 1:1000), mouse anti-BRCA1 (clones MS110 and MS13 Calbiochem ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-cleared by centrifugation and incubated with 25μl of mouse-anti-HA agarose beads (Millipore) or mouse-anti-FLAG M2 agarose affinity gel (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was incubated with primary antibody (mouse anti-FLAG antibody (Sigma, F1804; 1:1000); mouse anti-Tubulin (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were lysed using lysis buffer and immunoprecipitation with anti-FLAG M2 mouse (Sigma, F3165) as it was described above ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes underwent 6h incubation with primary antibodies: mouse α KCC2 (1:1000, Millipore; #07-432), rabbit α phospho-KCC2(S940 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured intact mouse or human islets were resuspended in 0.1mmol/L EGTA (Sigma-Adlrich, 234626) in serum free CRMLfollowed by transduction of indicated adenoviral constructs at 10,000 plaque-forming unit per islet equivalent (pfu/IEQ ...
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bioRxiv - Molecular Biology 2021Quote: ... IL-6 and TNFα using Milliplex Map Mouse Serum Adipokine Panel (Millipore catalogue # MADKMAG-71K) on a Luminex 200IS platform.
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate [EMD Millipore AP160P] ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were immunoblotted with the following antibodies: mouse anti-β-actin (AC-15, Sigma #A5441), mouse anti-β-catenin (cl14 ...
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bioRxiv - Molecular Biology 2020Quote: ... and mouse anti-β-actin conjugated to HRP (Sigma-Aldrich: A3854; RRID: AB_262011; 1:50,000).
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated with anti-mouse antibody (1:5,000; Merck Millipore, Germany; Cat. No. AP124P). After incubation ...
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bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
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bioRxiv - Immunology 2021Quote: ... mice were treated with deoxyribonuclease I (DNase I) from bovine pancreas (Sigma, 2000 units/mouse) or PBS vehicle control ...
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bioRxiv - Microbiology 2019Quote: ... Immunoblotting was performed with anti-Protein A mouse monoclonal primary antibody (SPA-27, Sigma Aldrich) or anti-LukS-PV mouse monoclonal primary antibody (IBT) ...
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bioRxiv - Immunology 2019Quote: ... the strips were further incubated for 2 h with goat anti-mouse IgG (Sigma-Aldrich) diluted 1:500 or goat anti-feline IgG (Southern Biotech ...
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bioRxiv - Neuroscience 2019Quote: ... and the mouse monoclonal anti-ß-Tubulin III (IgG2A) is from clone SDL.3D10 (Sigma). Both antibodies were used at a 1/500 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibodies used to probe PCNA immunoprecipitates included anti-FLAG (mouse monoclonal, Sigma, 1:1000 dil), anti-PCNA (rabbit ...
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bioRxiv - Biophysics 2019Quote: ... 1:200 (0.95 μg/ ml) and mouse anti-paxillin (clone 5H11, Millipore Cat # 05-417), 1:1000 ...