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Citations for Millipore Sigma :
7951 - 8000 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: HEK 293T cells were co-transfected with 8 µg pN3 HTLV-1 Gag and 0.8 µg HTLV-1 Env plasmids by using the polyethylenimine (PEI) transfection reagent (Sigma-Aldrich, MO) at a 1:3 weight to volume ratio ...
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bioRxiv - Immunology 2022Quote: Monoclonal antibody production was performed using 293-6E cells (National Research Council of Canada) by co-transfection of heavy and light chain expression plasmids using 25 kDa branched polyethylenimine (Sigma-Aldrich). Culture supernatants were harvested after an incubation period of 6-7 days at 37°C and 6% CO2 under constant shaking in FreeStyle Expression Medium supplemented with penicillin (20 U/ml ...
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bioRxiv - Biochemistry 2019Quote: ... 50 ng of envelope plasmid (pCMV-VSV-G) and 500 ng of pLKO vector containing the hairpin sequence (Sigma; Table S2) using TransIT-LT1 (Mirus Bio ...
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bioRxiv - Genomics 2019Quote: ... Individual colonies were cultured overnight before isolation of plasmid DNA using the GenElute™ miniprep kit (Sigma-Aldrich, Catalogue No. PLN350). Purified plasmids were Sanger sequenced to confirm successful cloning ...
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bioRxiv - Synthetic Biology 2019Quote: ... cell cultures containing the plasmids used in this study were grown overnight and 1mL aliquots were lysed using Cellytic tablets (Sigma-Aldrich). We mixed 30 μl of sample with 10 μl of 4x LDS loading buffer ...
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bioRxiv - Immunology 2019Quote: ... CRISPR-edited cell lines were generated by nu-cleofecting NK92 cells or CD34+ precursors with pCMV-Cas9-GFP all-in-one plasmid (Sigma-Aldrich) with the in-tronic guide sequence GAT TTA ATA TTC CCC GCT TGG G ...
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bioRxiv - Neuroscience 2020Quote: ... A mix containing 1 μg/μl endotoxin-free plasmid DNA (pCAG-mScarlet-i) plus 0.5% Fast Green (Sigma; 1:20 ratio) was injected into one lateral hemisphere ...
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bioRxiv - Neuroscience 2020Quote: ... was tagged with 6xHis at the C-terminus using PCR amplification and the modified Tau-His cassette was introduced into pET3a plasmid (Novagen, DE) linearized with NdeI enzyme succeeding in pET3a/htau40/6xHis plasmid for the protein expression in E.coli ...
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bioRxiv - Molecular Biology 2019Quote: ... coli as a fusion protein with the ketosteroid isomerase (KSI) using the pET31 plasmid (Kuliopulos et al., 1994) (Novagen/EMD Biosciences). KSI fusion proteins are concentrated in inclusion bodies in E ...
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bioRxiv - Molecular Biology 2020Quote: The 3×Flag-CPEB3 plasmid was generated by subcloning the PCR-amplified human CPEB3 cDNA into the p3×FLAG-CMV 10 vector (Sigma-Aldrich). The human CPEB3 cDNA was synthesized using the indicated primers ...
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bioRxiv - Genomics 2021Quote: ... 2 PCR replicates each using 1.5 µg of plasmid extracted library were amplified using custom barseq primers CJA120/CJA138 using KAPA2G Robust HotStart ReadyMix (Sigma 2GRHSRMKB) with the following conditions ...
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bioRxiv - Biochemistry 2021Quote: The pOP3MT plasmid containing the sequence of the mature form of SKD3 was transformed into Escherichia coli BL21(DE3) (Millipore-Sigma). Cells were cultured in 2 × 5 ml LB broth with 100 μ g/ml ampicillin for 16 hours at 30 °C with shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded into 96-well plates and transfected with E2F1 luciferase reporter plasmids (50 ng/well) using X-tremeGENE HP DNA Transfection Reagent (#06366236001, Sigma-Aldrich). After 12 hours ...
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bioRxiv - Plant Biology 2020Quote: ... Restriction free cloning of the RBS-AtRbcLScHis cassette was utilized to insert the cassette into the multiple cloning site 1 of the pRSFDuet™-1 plasmid (Novagen). To obtain single mutants ...
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bioRxiv - Immunology 2021Quote: ... The Mb3601-EGFP fluorescent strain were derived by electroporation with an integrative plasmid expressing EGFP and selected with Hygromycin B (50 μg/mL) (Sigma, USA) as described in previously [33] ...
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bioRxiv - Immunology 2020Quote: ... DH10β and STBL3 for propagation of plasmids were cultured at 37°C C (30°C C for STBL3) in LB broth (Sigma-Aldrich) with shaking at 220 rpm.
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bioRxiv - Genomics 2021Quote: Localization of resulting plasmids was assessed with immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... cDNA fragments of TopoIIα and TopoIIβ CTD were amplified from full length cDNA and then cloned in the pET3 0a plasmid (EMD Millipore/Novagen). The TopoIIα truncation mutants were generated using PCR ...
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bioRxiv - Immunology 2022Quote: ... and co-transfection of heavy and light chain expression plasmids into 293-6E cells (National Research Council of Canada) using 25 kDa branched polyethylenimine (Sigma-Aldrich). After 6-7 days at 37°C and 6% CO2 under continuous shaking in FreeStyle Expression Medium supplemented with 20 U/ml penicillin and 20 µg/ml streptomycin (all Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Ubi:caSMAD3 was cloned by PCR amplifying human constitutively active SMAD3 from Smad3 pCMV-SPORT6 (Harvard Plasmid Repository #HSCD00339271, Boston, MA, USA)(Sigma 11732641001), gel purifying (Qiagen #28604 ...
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bioRxiv - Cell Biology 2022Quote: ... the HEK293F GnTI-cells were transfected with a total of 1mg plasmid DNA (333 μg TFEB,400 μg RagA, and 267 μg RagC) and 4 mg polyethylenimine (PEI) (Sigma-Aldrich) per liter at a density of 1.5–1.8 × 106 cells/ml ...
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bioRxiv - Synthetic Biology 2022Quote: ... Each reaction was prepared as described previously 54 to contain 3.33 ng/μL pJL1-PD-4xDQNAT plasmid and 30% (vol./vol.%) extract in addition to: 10 mM magnesium glutamate (Sigma, 49605), 10 mM ammonium glutamate (Biosynth ...
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bioRxiv - Microbiology 2023Quote: Expression of heptose biosynthesis enzymes from inducible expression plasmids was carried out in Luria-Bertani broth (LB, Lennox [Oxoid]) medium supplemented with 50 µg/µL kanamycin (Sigma, USA), from expression cultures set up in E ...
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bioRxiv - Molecular Biology 2023Quote: Supernatant of HEK 293E cells overexpressing ADAMTS7 (or mock plasmid) and TIMP1 were collected and subjected to concentration using 3K Amicon Ultra-15 filters (Sigma-Aldrich). Recombinant MMP-9 from the Matrix Metalloproteinase-9 Colorimetric Drug Discovery Kit (Enzo Life Sciences ...
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bioRxiv - Microbiology 2024Quote: The 293T cells were seeded in a T75 cm2 flask (1 x 106) and the next day were transfected with plasmid DNA using GeneJuice transfection reagent (EMD Millipore) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The transfection plasmids were mixed in a 4:2:1 ratio in Opti-MEM medium utilizing the polycationic agent polyethylenimine (PEI; Sigma-Aldrich). Following transfection ...
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bioRxiv - Synthetic Biology 2022Quote: ... Yeast cultures for plasmid transformation and genome editing were grown in yeast extract-peptone-dextrose (YPD) medium (50 g/L YPD Broth (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were transfected in a 10-cm dish at 80-90% confluence with viral vectors containing each iMN factor and viral packaging plasmids (PIK-MLV-gp and pHDM for retrovirus, pPAX2 and VSVG for lentivirus) using polyethylenimine (PEI)(Sigma-Aldrich). The medium was changed 24 hours after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with pCG1-SARS-CoV-2_S plasmid using X-tremeGENE™ 9 DNA transfection reagent (Millipore-Sigma: 06365779001) (ratio 3:1 μl/μg DNA) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with pCG1-SARS-CoV-2_S plasmid using X-tremeGENE™ 9 DNA transfection reagent (Millipore-Sigma: 06365779001) (ratio 3:1 μl/μg DNA) ...
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bioRxiv - Plant Biology 2023Quote: ... together with the p35S:nlsCFP (as a nuclear marker) plasmid (Table S4) were grown overnight at 28°C in liquid LB broth containing 50 μg/mL kanamycin (Sigma-Aldrich), 25 μg/mL gentamicin (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... Truncated FBF expression constructs were generated by PCR from full-length FBF-1 or FBF-2 and inserted into pETDuet-1 plasmid (EMD Millipore). Full-length C ...
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bioRxiv - Microbiology 2023Quote: ... The cassette corresponding the DHFR-pSAG1-TgAPT1-GFP cassette was amplified by PCR from the plasmid of the same name using primers ML3088/ML3089 with the KOD DNA polymerase (Sigma-Aldrich). This donor sequence was then used to transfected to Pru background ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells stably expressing the plasmid Tet-On mRFP–EGFP Golgi were cultured with 2 μg/mL doxycycline (D3447; Sigma-Aldrich) for 24 h ...
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bioRxiv - Pathology 2023Quote: ... The cells were infected with lentiviruses carrying the plasmid (MOI = 6) in the presence of Hexamethidine Bromide (8 µg/mL; Sigma-H9268) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... coli JKE201 carrying the plasmid of interest was cultivated for 16-20 hours at 37 °C in LB with 500µM DAP (Sigma, LB-DAP) and 30 µg/ml Chloramphenicol ...
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bioRxiv - Genetics 2022Quote: ... T4 ligations into multiple cloning site plasmids were performed by amplifying fragments via PCR with Roche Expand High Fidelity Polymerase (Sigma 11732641001) with primers containing 5’ restriction enzyme sequences for digestion following PCR purification ...
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bioRxiv - Systems Biology 2023Quote: ... The pRS3.R11_lacI series plasmid harbouring the nahR-Psali expression system57 which can respond to the sodium salicylate (Sigma-Aldrich, S3007) was used to titrate the expression level of LacI ...
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bioRxiv - Microbiology 2023Quote: ... The cell suspension was mixed with 10 µg plasmid and transferred to a chilled 2 mm electroporation cuvette (Sigma-Aldrich, USA). The cells were subjected to electroporation treatment using a BioRad Gene Pulser Xcell (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... HC-04 cells expressing CAS9 were generated using lentivirus containing the EF1a-Cas9-2A-Blasticidin Lenti plasmid (Sigma Aldrich, #LVCAS9BST-1EA). HC-04 cells were plated at a density of 120,000 cells per well in three independent wells of a 24 well plate ...
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bioRxiv - Cancer Biology 2022Quote: CRISPR/Cas9 gene deletion of CNTNAP4 in human OS cell lines and primary cells was achieved using a plasmid with a guide RNA (gRNA) sequence combined with U6gRNA-Cas9-2A-RFP vector (Sigma-Aldrich). The specific gRNA sequence of KO and negative control plasmids used in this study is shown in Supplementary Table 3 ...
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bioRxiv - Biophysics 2022Quote: ... was introduced using appropriate mutagenic oligonucleotides and a protocol for whole plasmid site-directed mutagenesis employing KOD polymerase (Merck Millipore, Germany) as detailed in manufacturer’s instructions and previous work (Zheng et al. ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal antibodies were produced in 50 ml of 0.8×106 HEK293-6E cells by co-transfection of heavy chain (IgG1) and light chain (Igλ, Igκ) antibody expression plasmids with polyethylenimine (PEI; Sigma-Aldrich). Cells were maintained at 37°C and 5% CO2 in FreeStyle 293 Expression Medium (Thermo-Fisher ...
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bioRxiv - Cancer Biology 2023Quote: All lentiviral preparations were made via co-transfection of target and packaging plasmids in HEK293T cells using the XtremeGENE HP DNA transfection reagent (Sigma, #6366236001). Packaging plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected with 1 µg of ATP5I-SNAP (Kondadi et al., 2020a) or 1 µg of mitGoTeam2 plasmid DNA using GeneJuice® (Novagen) reagent for 48 hours according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... were transformed with pET-24a plasmid coding for His6-SUMO S100A13 (UniProt Q99584) and grown in high salt LB-broth medium (Sigma Aldrich). Cells were grown until an OD600 of 0.6-0.8 and expression induced with 0.1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) ...
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bioRxiv - Molecular Biology 2023Quote: ... After the overnight growth at 37 °C, the cells were harvested by centrifugation (30 min, 4300 g) and plasmid purified by midi-prep (Millipore Sigma).
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bioRxiv - Molecular Biology 2023Quote: ... After overnight growth at 37 °C, each of the 50 mL cultures were centrifuged (30 min, 4300 g) and plasmid purified by midi prep (Millipore Sigma). The bottlenecked library displayed an estimated 16.9 fold barcode/variant coverage.
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bioRxiv - Developmental Biology 2023Quote: ... Full lengthrfx6.L and pdx1.L anti-sense DIG-labeled in-situ probes were generated using linearized plasmid cDNA templates with 10× DIG RNA labeling mix (Sigma 11277073910) according to the manufacturer’s instructions.
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... dCas9-VP64-blasticidin SAM CRISPRa helper construct 1 plasmid DNA (cat#: SAMVP64BST) and MS2-P65-HSF1-hygromycin SAM CRISPRa helper construct 2 plasmid DNA (cat#: SAMMS2HYG) were purchased from Sigma-Aldrich. Puromycin (cat# ...