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Citations for Millipore Sigma :
8001 - 8050 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 40ul of 20mg/mL X-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside – Sigma Aldrich B44252) and 40ul of 100mM IPTG (isopropyl β-D-1-thiogalactopyranoside – AppliChem A4773 ...
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bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in 5 µL of cold lysis buffer (10 mM Tris-HCl [pH 7.4], 10 nM NaCl, 3 nM MgCl2, 0,1% v/v Igepal CA-360 [Sigma-Aldrich] and incubated on ice for 5 min ...
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bioRxiv - Bioengineering 2021Quote: Sections were incubated in a permeabilization and blocking solution of 0.3% Triton X (A4975, PanREAC AppliChem) and 3% BSA (A7906, Sigma) in PBS for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane, 2 membranes per animal, 0.4 μm Millicell, Merck Millipore) in 50% BME (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... 10 ml of alkaline phosphatase (100mM Tris-HCl pH 9.5, 100 mM NaCl, 3 mM MgCl2) containing 80 μl NBT (Sigma) (35mg/ml in70% DMSO and 30 μl de BCIP (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at 4°C in a humid chamber with primary antibodies (Table 3) in 0.1 M PBS containing 10% normal horse/goat serum and PBS-0.3% Triton X-100 (Sigma-Aldrich). After primary antibody incubation ...
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bioRxiv - Cell Biology 2021Quote: ... 1uM each of gRNA (IDT) were mixed with spCas9 at 3:2 ratio in embryo grade water (W1503, Sigma) and injected into wild type C57BL6 zygotes at one-cell stage ...
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bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Bioengineering 2021Quote: ... pH 7.4 by centrifugation through 3 kDa molecular weight cut off centrifugal filters (Amicon Ultra®, Millipore, Dublin, Ireland). Absorbance at 555 and 280 nm was measured for labelled samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 to enable quantification of relative protein concentration and label substitution efficiency ...
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bioRxiv - Bioengineering 2020Quote: ... cells (15·106 mFBs·cm−3 and 30·106 THP-1 cells·cm−3) were added to a mixture of bovine fibrinogen (10 mg·ml−1; Sigma, F8630) and bovine thrombin (10 IU·ml−1 ...
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bioRxiv - Bioengineering 2020Quote: ... we added 3% v/v photoinitiator Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (Sigma-Aldrich, “LAP”, 39.2 mg/mL in DI water). Produced Ln/polymer droplets were collected through Tygon tubing into a 24-well plate (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... after a 60 min incubation at 25 °C the reactions were stopped by addition of 3 μl 3.3 × LDS sample buffer (Sigma) containing NEM (40 mM final concentration ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with Oil Red O working solution (3:2 Oil Red O: ultrapure water) for 20 minutes (Sigma O1391), and washed with 5 changes of ultrapure water.
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bioRxiv - Neuroscience 2020Quote: ... Cells were released via 0.12% trypsin without EDTA and washed 3 times with PBS (Sigma-Aldrich, St. Louis, MO). 0.4% trypan blue (Thermofisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Adult zebrafish (6 female and 6 male) were anesthetized in 0.02% tricaine (E10521, Ethyl 3-aminobenzoate methanesulfonate; Sigma-Aldrich). Shallow lesions were made on the skin (10 on each side ...
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bioRxiv - Neuroscience 2020Quote: ... A 96-well PCR plate was prepared with one 3 mm diameter borosilicate solid-glass bead (Millipore Sigma #Z143928) and 100 µl PBS in each well ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately fused to 18 mm glass coverslips (#1.5) that had been treated with 3-aminopropyl-triethoxysilane (Sigma 440140). Samples were washed with PBS 3x 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... The rest was incubated with 30μl of Protein G Dynabeads and 3 μl of anti-FLAG M2 antibody (Sigma) overnight to immunoprecipitate (IP ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was determined using the 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, Milan, Italy) according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2021Quote: ... gRNA sequence: 5’-ACTTGGCCAAGGAGTACGG-3’) in line with GFP from the (p04) U6-gRNA:CMV-eCas9-2a-tGFP plasmid (Sigma). One day later ...
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bioRxiv - Cell Biology 2021Quote: ... and cells were isolated by digesting the pulp tissue with collagenase type I (3 mg/ml, Sigma-Aldrich, Germany) for 60 minutes at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... the culture medium was removed and cells were rinsed 3 times with Dulbecco’s Phosphate Buffered Saline (DPBS, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... and 1% Glycerol) containing a 3-fold dilutions (from 0.125 units/ml to 6 units/ml) of Benzonase nuclease (Millipore). This was mixed gently and incubated for three minutes at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2 6 H2O and 5-Bromo-4-chloro-3-indolyl β-D-galactoside or X-gal (Sigma, B4252 ...
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bioRxiv - Cancer Biology 2020Quote: ... CM (and the same volume of SM as control) was then concentrated by 3 kDa Amicon filters (Merck-Millipore) to a final volume of 100 μl/mouse and stored at −20°C until usage ...
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bioRxiv - Cancer Biology 2020Quote: ... Viability was evaluated with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) colorimetric assay (Sigma, #M5655).
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bioRxiv - Cell Biology 2021Quote: ... The target cell lines at 50-60% confluency were then infected using 3 mL virus with polybrene reagent (Sigma). After 24 hours ...
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X-100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Mice were fed ad libitum or fasted at ZT0 and given CL316243 (3 mg/kg body weight; Sigma Aldrich) or vehicle (0.5% carboxymethylcellulose in sterilized water ...
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bioRxiv - Plant Biology 2020Quote: ... epitopes were blocked with 0.1 M PBS / 1% Cold Water Fish Skin Gelatin / 0.2% BSA with 3% sucrose (all chemicals from Sigma Aldrich), incubated with the primary antibody anti-GFP (1:250 ...
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bioRxiv - Biochemistry 2021Quote: ... The C-terminal domain (CTD) of DRH-3 (residues 940-1108) was cloned into the expression vector pET15b (Novagen), and the resulting clone carries an N-terminal His6 tag and a thrombin cleavage site ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM sodium phosphate pH 8.0, 3% glycerol, 1% Triton X-100, 15 mM imidazole, and 1x protease inhibitor [Roche, Sigma]) and sonicated ...
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bioRxiv - Biochemistry 2020Quote: ... each N-Cdh sample was desalted against PBS using Amicon centrifugal filters with a 3 kDa molecular weight cutoff according to the manufacturer’s protocol (Merck-Millipore). Unprocessed xCGE-LIG N-glycocomics raw data are available upon request.
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bioRxiv - Bioengineering 2021Quote: ... 0.1% solution of Triton X-100 in PBS (v/v) and 3% Bovine Serum Albumin solution (Sigma Aldrich, A3803) in PBS (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... the surfaces of the coverslips were amino-modified with 3-(Trimethoxysilyl)propyl methacrylate (#M6514, Sigma-Aldrich, Saint-Louis, USA) to allow firm gel attachment ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were then rinsed 3 times with PBS followed by permeabilization with 0.1% triton X-100 (Sigma, X100) in PBS for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... cells were trypsinized than fixed with PBS/ formalin 3% then incubated with PBS/ Triton 0.1%/ TRITC-phalloïdin 1 µg/mL (Sigma Aldrich) during 5 min ...
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bioRxiv - Cell Biology 2021Quote: For analysing autophagy flux 72-hours old larvae were transferred to fresh medium containing 3 mg/ml chloroquine (Sigma) as described previously (Low et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Polysomes were eluted by resuspension of the washed gel in polysome wash buffer containing 3×FLAG peptide (Sigma-Aldrich). Total RNA was extracted from the final elution using Direct-zol RNA kit (Zymo research) ...
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bioRxiv - Plant Biology 2020Quote: ... Trypsin digestion was stopped either by heating at 95 °C for 3 min or by ultrafiltration using 0.5 ml Amicon Ultra centrifugal devices (20 kDa MWCO, Millipore). A five microliters aliquot was kept for MALDI-TOF MS analysis and the rest was used for the enrichment of biotinylated peptides by affinity chromatography.
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bioRxiv - Plant Biology 2021Quote: ... Col-0 plants grown on soil for 3-4 weeks were spray-treated with 0.5 mM SA (Sigma-Aldrich) and tissues harvested at indicated time-points for further analysis.
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bioRxiv - Plant Biology 2020Quote: ... The algal suspension was mixed into an equal volume of 3% (w/v) sodium alginate (Sigma, Product number 180947) solution then a syringe was used to generate 3 – 4 mm diameter algal-alginate beads by adding drops of the sodium alginate-algal mixture into a 2% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... The remaining 200 µL was transferred to a 3 kDa Amicon Ultra 0.5 mL filtering device (Millipore, Burlington, MA) for the quantification of AAs ...
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bioRxiv - Microbiology 2021Quote: ... amoebae were left unstained and incubated with either 3×106 or 1.5×107 fluorescent red carboxylate-modified polystyrene 2.0 μm latex beads (Sigma-Aldrich) or incubated in the absence of latex beads ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then washed 3 times with PBST before the addition of 100ul of .5mg/ml o-Phenylenediamine (Sigma) diluted in phosphate-citrate buffer with sodium perborate (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... were immobilized on freshly made 3% agarose pad in a drop of M9 media containing 0.4 mM (0.05%) tetramisole (Sigma Aldrich) and 3.8 mM (0.5% ...
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bioRxiv - Microbiology 2021Quote: ... 50 µg of purified Cu-LpmP or CslZ protein were incubated for 3 h at room temperature with 0.2% peptidoglycan (PG) from Bacillus subtilis (Sigma), 5 mg α-chitin from shrimp shells (Sigma ...