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Citations for Millipore Sigma :
1001 - 1050 of 10000+ citations for Protein DBF4 Homolog A DBF4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... GFP antibodies (Sigma) and Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate antibody were used ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibody (Millipore); and anti-mouse NeuN (1:1000 for 12 h ...
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bioRxiv - Neuroscience 2020Quote: ... SST antibody (Millipore, #MAB354 ...
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bioRxiv - Molecular Biology 2019Quote: ... S9.6 antibody (Millipore) was added and samples were incubated overnight at 4° C with gentle rotation ...
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bioRxiv - Microbiology 2022Quote: ... αFLAG antibody (Sigma) was used at 1:10,000 to detect Cap2-3×FLAG ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15, MWCO 10k; Millipore), and after adding glycerol to 20% final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... protein concentration was routinely determined by densitometry of Sypro-Orange stained gels against a calibration curve with protein standards (Sigma) using ImageJ ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G and Protein A Dynabeads (1:1 mixed) were blocked with 1mg/ml denatured yeast tRNA (Sigma-Aldrich, R5636) and 1mg/ml of BSA (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... For the ChIP using SV40 chromatin isolated 30 minutes post-infection we substituted a mixture of protein A and protein G magnetic beads (16-663, Millipore) for the protein A and protein G agarose which we previously used in our publications ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Microbiology 2020Quote: ... Protein-protein interactions in situ were visualized using the Duolink® in situ proximity ligation assay (PLA) system (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Immunology 2021Quote: ... Proteins smaller than 30 kDa were removed by passing the protein lysate through an Amicon Ultra - 15 Centrifugal Filter (Millipore). One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz ...
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bioRxiv - Biophysics 2020Quote: ... For reduction and alkylation of the proteins, proteins were incubated with SDC buffer (1% Sodiumdeoxycholate, 40nmM 2-Cloroacetamide (Sigma-Aldrich), 10 mM tris(2-carboxyethyl ...
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bioRxiv - Molecular Biology 2019Quote: Purified proteins were concentrated to the indicated protein concentration described in the text using Amicon Ultra centrifugal filters (10K MWCO, Millipore) and added to solutions at varying concentrations with the indic ated final salt and molecular crowder concentrations ...
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bioRxiv - Cell Biology 2019Quote: ... Bcl2/Adenovirus E1B 19 kDa and protein-interacting protein 3-like (BNIP3L/NIX) from Sigma-Aldrich (St. Louis, MO, USA), perilipin (PLIN ...
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bioRxiv - Cell Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore; endogenous RNA pol II IPs) or GFP-Trap®_A beads (Chromotek ...
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bioRxiv - Cancer Biology 2022Quote: An in situ proximity ligation assay was performed to identify protein-protein interactions in cells using a Duolink In Situ Red Starter Kit (Sigma) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 40μg of total protein were loaded per well in SDS-acrylamide/bisacrylamide gel and proteins were electrophoretically transferred to a PVDF membrane (Millipore). For treatment with MG132 and ABA ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were loaded onto HisTrap column and flowthrough containing cleaved protein was pooled and concentrated (Amicon 3 K Sigma Aldrich). Proteins were loaded onto a Superdex 200 increase 10/300 GL column (Cytiva ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Protein concentration in the samples was measured using the Bicinchoninic Acid Protein Assay Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Immunology 2023Quote: ... The protein concentration in the supernatant was measured by the BCA protein assay kit (Sigma-Aldrich, cat. No. BCA1-1KT).
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bioRxiv - Physiology 2023Quote: ... All images were normalized total protein from the same membrane stained using Amido Black total protein-stained membrane (A8181, Sigma).
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bioRxiv - Cell Biology 2023Quote: ... Instrument performance was monitored daily using a system suitability test data consisting of a 30-minute gradient injection of 30fm BSA and 60fm of casein protein digest (both standards were trypsin digested in-house from commercial protein stocks (Sigma)) that was run before each sample ...
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bioRxiv - Neuroscience 2023Quote: ... and 50 µL was taken as protein input while the remaining lysate was pre-cleared using 100 µL or Protein G Sepharose beads (Sigma) at 4°C for 30 min ...
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bioRxiv - Physiology 2023Quote: ... Supernatants containing equal amounts of soluble proteins were mixed with 2x SDS protein sample buffer containing 100mM DTT (Sigma-Aldrich), denatured by incubating on a heat block at 98 C for 5 minutes ...
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bioRxiv - Genetics 2023Quote: ... cOmplete proteinase inhibitor) to remove nonspecific-binding proteins and then HA-tagged proteins were eluted with HA peptides (Sigma-Aldrich). WT samples (not HA tagged ...
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bioRxiv - Immunology 2024Quote: ... 20 μg cell proteins or 3-5 μg EVs proteins were denatured in LDS sample buffer containing 1M Dithiothreitol (DTT, Sigma) for 10 min at 70°C and loaded onto a 4-12% precast polyacrylamide gels for electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used were mouse anti-β-actin monoclonal antibody (Sigma, catalog number A5441 ...
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bioRxiv - Microbiology 2019Quote: ... The following antibodies were used: anti-Flag antibody (1:2,000; Sigma), anti-VSVG antibody (1:2,000 ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies were detected using fluorochrome-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:200 in 1% Triton-X100 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used are: tyrosine hydroxylase (TH) antibody (rabbit polyclonal, Millipore) for noradrenergic (NAergic ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Biochemistry 2020Quote: ... The following antibodies were used: anti-PLK4 antibody clone 6H5 (Millipore, used at 1/100 for western blot and immunofluorescence) ...
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A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... primary antibody and goat anti-mouse peroxidase secondary antibody (Sigma A4416), and imaged on an Amersham ImageQuant800 (GE Lifesciences).
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bioRxiv - Cell Biology 2021Quote: ... Bound antibody was detected using peroxidase-conjugated secondary antibodies (Sigma-Aldrich Company Ltd ...
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bioRxiv - Molecular Biology 2021Quote: ... and Western blotting performed using CFTR antibodies or actin antibody (Millipore). The CFTR antibody utilized for full-length protein was 596 against amino acids 1204-1211 (UNC School of Medicine) ...
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bioRxiv - Cancer Biology 2022Quote: ... GAPDH antibody (MAB374) and Cas9 antibody (MAC133) were purchased from Millipore. HSP72 (ADI-SPA-810 ...
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bioRxiv - Neuroscience 2020Quote: ... Two antibodies were used: a monoclonal mouse anti-S100β antibody (Sigma S2532 ...
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bioRxiv - Biochemistry 2020Quote: Mouse anti-FLAG M1 antibody and mouse anti-Sulfotyrosine antibody (Sigma) were used to detect the purified wild type C5aR and C5aRΔTyr with Y11F and F14F mutations respectively in the western blotting assays.
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bioRxiv - Microbiology 2020Quote: ... β-actin antibody and C9 antibody were purchased from Sigma (A2228) and SANTA CRUZ (sc-57432) ...
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bioRxiv - Microbiology 2021Quote: ... coupled to antibodies (anti-pan Ago antibody, clone 2A8; Sigma Millipore). 10% of supernatants were kept for input (in TRIzol) ...
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bioRxiv - Microbiology 2021Quote: ... coupled to antibodies (anti-pan Ago antibody, clone 2A8; Sigma Millipore). 10% of supernatants were kept for input (in TRIzol) ...
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bioRxiv - Immunology 2020Quote: An anti-SP140 antibody produced in rabbit (HPA006162; Sigma Prestige Antibody) was used to visualize SP140 protein in a Cambridge Bioscience 69571061 Normal Human tissue microarray ...
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bioRxiv - Genomics 2021Quote: ... The secondary antibody (Anti-Rabbit IgG antibody, Goat monoclonal: Millipore AP132) was diluted to 1:100 in the dig wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal antibody against α-tubulin antibody (1:200; T6074, Sigma) and guinea pig polyclonal antibody anti-CenpC antibody (1:200 ...