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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Naïve B cells were activated by addition of 5-25 μg/ml LPS (Sigma-Aldrich) and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Stable cells were generated by selection with 800µg/mL of Hygromycin B (Sigma-Aldrich, H7772). The cells were again transfected with the SBE-RFP reporter with the piggyback transposase and selected for stable integration of both reporters by three rounds of fluorescence-assisted cell sorting for YFP and RFP double positive cells (dual reporter cells).
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested by trypsinization and washed with ice-cold PBS-B (DPBS (Sigma-Aldrich) containing 1% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell culture medium was supplemented with 2 μg/ml of cytochalasin B (Sigma-Aldrich, #C6762) 16 hrs prior to fixation to block cells at cytokinesis stage of cell division ...
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bioRxiv - Molecular Biology 2020Quote: ... and mRNA sequence specific primers (Sigma-Aldrich). The qPCR was performed with 2 μL of a 1:10 dilution of the cDNA for the genes ACT1 ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-rabbit light chain specific (Millipore MAB201P) and anti-goat (Invitrogen A27014 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-ubiquitin Lys48-specific (Millipore #05-1307), anti-ubiquitin Lys63-specific (AbCam #ab179434) ...
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bioRxiv - Cancer Biology 2019Quote: ... and Lys48-Specific antibody (05-1307, Millipore). Secondary antibodies are from Dako (P0447 and P0399) ...
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bioRxiv - Plant Biology 2019Quote: ... a specific proteasome inhibitor (E3652, Sigma-Aldrich) were used as controls ...
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bioRxiv - Microbiology 2021Quote: ... or GFP-specific antibodies (Sigma, cat# G6539) were washed twice with lysis buffer and then mixed with the prepared cell supernatant ...
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bioRxiv - Immunology 2019Quote: ... or a specific target molecule (Sigma-Aldrich) was transfected into HEK293T cells together with psPAX2 and pMD2.G with Lipofectamine 2000 ...
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bioRxiv - Immunology 2020Quote: ... or a specific target molecule (Sigma-Aldrich) was transfected into HEK293T cells together with psPAX2 and pMD2.G with Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2020Quote: ... H3K9me3 rabbit polyclonal (Abcam ab8848),BG4 G4 specific antibody monoclonal (Millipore MABE917), EZH2 rabbit polyclonal (CST 4905),GAPDH mouse monoclonal (Santacruz 6C5).TRF1 mouse monoclonal (Novus NB110-68281) ...
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bioRxiv - Immunology 2020Quote: ... RNF20 or CtIP+RNF20-specific shRNAs (Sigma) and then placed cells under puromycin selection (0.5 μg/ml) ...
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bioRxiv - Biochemistry 2022Quote: ... or scramble non-specific siRNA (Sigma Aldrich St ...
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bioRxiv - Microbiology 2023Quote: ... with gene-specific primers (Sigma, Bangalore, India) (Table 1 ...
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bioRxiv - Plant Biology 2023Quote: ... Plant specific protease inhibitor (Sigma-Aldrich P9599), and cOmplete Protease Inhibitor Cocktail EDTA-free (Roche) ...
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bioRxiv - Bioengineering 2024Quote: Specific concentrations of antibiotics: gentamicin (Sigma-aldrich) and oxacillin were prepared by diluting the starting stock concentration of 5 mg/mL using TSB broth ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mg/mL of 70 kDa dextran conjugated to rhodamine b isothiocyanate (Rhod B, Sigma) was introduced from one media reservoir ...
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bioRxiv - Immunology 2020Quote: ... For experiments using the LPS inhibitor polymyxin B (PMB, Polymyxin B sulfate salt, Sigma-Aldrich), the samples were mixed with PMB and incubated for 30 min at RT before being added to the cultivated cells ...
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bioRxiv - Plant Biology 2023Quote: ... protein expression in E.coli Rosetta cells (Novagen) was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were incubated with anti-mouse minus and anti-rabbit plus PLA probes (PLA kit from Sigma) at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: Mouse High Sensitivity T Cell Magnetic Bead kits were purchased from EMD Millipore (MHSTCMAG-70KPMX, Burlington, MA, USA) and used to quantify 18 different mouse cytokines in accordance with the manufacturer’s recommendations (belonging to 4 main categories ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
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bioRxiv - Neuroscience 2021Quote: ... HEK 293 cell edges were stained with Phalloidine-Atto565 (specific against F-actin) dye (Sigma–Aldrich, Switzerland). Nuclei were counterstained with DAPI (4′,6-diamidine-2′-phenylindole dihydrochloride ...
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bioRxiv - Microbiology 2021Quote: ... ICAM-1kd PLB985 cells were generated by lentiviral transduction with ICAM-1 specific shRNAs from Sigma (TRCN0000372478). EPCRkd PLB985 cells were generated by lentiviral transduction with EPCR specific shRNAs from Sigma (TRCN0000300553) ...
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bioRxiv - Microbiology 2023Quote: ... Fixed cells were probed for puromycin incorporation using a puromycin-specific antibody (1:1000, MABE343, EMD Millipore). The amount of puromycin incorporated was quantified using the method described above for infectivity measurement using LI-COR Odyssey.
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Biochemistry 2020Quote: ... For ELISAs with asialofetuin (Sigma Aldrich # A4781), 5 μg/mL was immobilized ...
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bioRxiv - Physiology 2023Quote: ... Insulin was measured by ELISA (Millipore Sigma). For glucose tolerance tests ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used for immunofluorescence on whole-mounted retinal sections were brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID:AB_94166) at 1:1000 and GFAP (1:1000) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Neuroscience 2023Quote: Whole retinas were flat-mounted on slides coated with Gatenby’s solution and stained sequentially using the following primary antibodies: brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID: AB_94166) at 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: Knockdown of proteins was achieved through RNA interference either by CaCl2 transfection of gene-specific shRNA encoding vectors (pLKO.1; Sigma-Aldrich or shRNAmiR) on DIV 7 or by transduction of cells with lentiviral particles harboring the gene-specific shRNA on DIV 2 ...
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bioRxiv - Immunology 2022Quote: ... IL-10 and TNF-α were determined in 24 h culture supernatant of UNT control and treated MOs using respective ELISA kits (Sigma-Aldrich kit, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and the protein level was measured with the Enhanced BCA Protein Assay kit (Sigma-Aldrich). The extracts which contained 25 μg of total proteins were subjected to 10% SDS polyacrylamide gel ...
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bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using a BCA protein concentration assay kit (Sigma, Shanghai, China, FP0010). Equal amounts of protein (20 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... the concentration of EV protein was quantified by using a BCA Protein Assay Kit (Novagen). For BMDC stimulation by EVs ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein-protein interaction was identified using a commercially available PLA kit (Duolink; Sigma-Aldrich) as described before 34 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein levels were then quantified using a Coomassie protein assay kit (B8522, Sigma-Aldrich, USA) followed by 12% SDS-PAGE separation (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then normalized to 6 μg protein and the KGDH assay kit (Sigma) protocol was followed.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined by Bicinchoninic Acid Kit for Protein Determination (BCA1-1KT, Sigma Aldrich) or the Bradford Protein Assay (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein interaction was investigated using a commercially available PLA kit (Duolink; Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein concentration in the supernatant was measured using the BCA Protein Assay kit (Sigma).
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bioRxiv - Microbiology 2021Quote: ... the following were incubated for 15-18hrs at 4°C in PBS with 0.3% Triton-X and 5% goat serum: mouse biotin conjugated anti-NeuN B-clone A60 (MAB377, Millipore, 1:500), rabbit anti-calbindin (C2724 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in 5% dry milk power in PBS for 1 hour at RT and incubated overnight at 4°C in primary antibody (anti-Lamin B1 1µg/ml, Abcam ab16048, Cambridge, UK; anti-PCA NCAM 1:500, Millipore MAB5324 ...