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Citations for Millipore Sigma :
751 - 800 of 820 citations for HVEM Rhesus Macaque HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Freshly isolated PBMCs from four donor M1/M2 MCM were pooled and resuspended in RPMI-1640 containing 10% fetal calf serum (FCS; R10) and 5 ug/ml phytohemagglutinin-P (Sigma-Aldrich). The culture was incubated overnight at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2020Quote: ... and 2.5 ng/mL TNF-α for 3-5 days and subsequent differentiation in RPMI (Sigma, St Louis, Mo; +10% FCS) either with 100 ng/mL GM-CSF ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Immunology 2022Quote: ... fibroblasts and MCF 10A cells were incubated with the FITC bound anti-mouse IgG secondary immunoglobulin (Fc specific) (Cat # F4143, Sigma-Aldrich) secondary antibody for 2 h at room temperature away from light ...
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bioRxiv - Cell Biology 2022Quote: ... A HeLa cell line stably expressing connexin43 [47] grown in DMEM/10%FCS/P/S supplemented with 1 μg/ml puromycin (Sigma #P9620) were solubilized in 1% Chaps/TBS/5% glycerol at pH 7.4 supplemented with protease blockers ...
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bioRxiv - Synthetic Biology 2022Quote: ... Single-cell suspensions were derived by mechanical disruption of bone marrow in PBS supplemented with 2% Fetal Calf Serum (FCS, Sigma, C8056). For FACS analysis ...
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bioRxiv - Microbiology 2022Quote: ... and suspended at 2 x 104 cells/mL in Titan Cell Medium (TCM) composed by 5% Sabouraud, 5% inactivated Fetal Calf Serum (FCS, Biological Industries) and 15 µM sodium azide (Sigma Aldrich), diluted in 50 mM MOPS adjusted to pH 7.3 ...
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bioRxiv - Microbiology 2022Quote: MPXV strains were diluted ½ in two different kinds of media that is Viral Transport Media (VTM; 330C, Copan) and FCS (F2442, Sigma-Aldrich). Two hundreds microliters of each sample were submitted in triplicate to various temperatures for different periods of time ...
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bioRxiv - Biochemistry 2022Quote: ... under agitation at room temperature for 20 min and re-probed with HRP-conjugated poly-clonal goat anti-mouse IgG Fc (1:5000 dilution, Sigma–Aldrich) for checking the integrity of the mouse IgG2b Fc domain of the fusion protein ...
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bioRxiv - Immunology 2023Quote: ... guts were transferred to a new 50 mL tubes containing 1x digestion mix (RPMI GlutaMAX™ containing 2% FCS, 1mg/mL of Colagenase VIII [Sigma] and 40 u/mL of DNAse I [Sigma]) and incubated for 20 min at 37 °C sin an orbital shaker at 200 rpm ...
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bioRxiv - Immunology 2023Quote: ... before counting in complete RMPI (cRPMI = naRPMI supplemented with 10% heat-inactivated FCS [#S-FBS-EU-015, Serana, Pessin, Germany], 25 nM β-mercaptoethanol [#M6250, Sigma] ...
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bioRxiv - Genetics 2023Quote: ... the tissue slurry was seeded directly on Matrigel coated dishes (45 μg/cm2) in DMEM/F12 containing 20%FCS (Sigma-Aldrich) and 10% horse serum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... ∼8 x 106 parasites were pelleted by centrifugation at 800 rcf for 7 min and resuspended in FCS-free HMI-9 media with 1 µg/mL Hoechst 33342 (Sigma-Aldrich). Cells were pelleted again ...
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bioRxiv - Immunology 2023Quote: ... Cells were re-suspended at 1×107 cells/ml in RPMI 1640 buffer supplemented with 10% foetal calf serum (FCS, Sigma-Aldrich) for further analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... AML cells were washed and cultured in Gartner’s medium (Alpha-MEM, 12.5% horse serum, 12.5% FCS, 1μM hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA), 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... The tissue was incubated in RPMI/5%FCS/10mMHEPES for 5min before being digested in 10ml RPMI/5% FCS/10mM HEPES supplemented with 0.4mg/ml type VIII collagenase (Sigma Aldrich, UK) and 40μg/ml DNase I (Roche ...
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bioRxiv - Immunology 2023Quote: ... samples were serially diluted in MEM containing 2% FCS and 100 Units Penicillin / 0.1 mg Streptomycin (P/S) (Millipore Sigma, Germany). Vero E6 cells were incubated with 100 µl of ten-fold sample dilutions added in quadruplicates for 1 h at 37°C before 100 µl MEM containing 2% FCS and P/S were added per well and plates were incubated for 5 days at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... THP1 dual reporter cells were maintained in RPMI (Nacalai Tesque Inc.,Kyoto, Japan) supplemented with 10% FCS (Sigma-Aldrich, Missouri, USA), 1% penicillin/streptomycin (Nacalai Tesque Inc.,Kyoto ...
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bioRxiv - Cancer Biology 2023Quote: ... Newly diagnosed leukemic cells was partly used directly after sampling for in vitro and in vivo experiments and partly frozen in Foetal Calf Serum (FCS) containing 10% of Dimethylsufoxide (DMSO) (D8418, Sigma-Aldrich). Human T-ALL samples are described in Supplementary Table 1.
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bioRxiv - Cancer Biology 2023Quote: ... The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO) using a syringe pump (Brandel) containing Fluorinert FC-40 (Sigma #F9755) at a speed of 0.75 ml/min ...
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bioRxiv - Immunology 2023Quote: ... were co-cultured in round-bottomed 96-well plates in DC medium (RPMI-1640, 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) the presence of indicated concentrations of OVA323-339 peptide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Isolates were standardised to 1×106 cells/mL in Roswell Park Memorial Institute (RPMI) media supplemented with 5% (v/v) foetal calf serum (FCS [Sigma-Aldrich]) to simulate organic load ...
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bioRxiv - Microbiology 2024Quote: ... BEAS-2B cells grown in 6-wells plates were first transduced with lentiviruses encoding Cas9 diluted in infection medium (DMEM, 2% FCS) containing 8 μg/ml Polybrene (Sigma Aldrich). At 16 h post transduction ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13-c6 was grown in 200mL of BMGY media supplemented 1tab/50mL of cOmplete Protease Inhibitor Cocktail (Sigma; 11836145001) and 10uM Pepstatin A (Sigma ...
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bioRxiv - Immunology 2023Quote: ... These PBMCs were then re-suspended in large volumes of RPMI 1640 medium containing 5% v/v fetal calf serum (FCS) (F-0926, Sigma, USA) and centrifuged against at 500 x g and after a further wash their PMBCs were re-suspended in ice-cold 10% dimethyl sulfoxide (D-2650 ...
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bioRxiv - Molecular Biology 2024Quote: ... Negative selection was therefore carried out by culturing the parasites in the presence of 5-fluorocytosine (5-FC, Sigma-Aldrich, UK) at 48 mM and 2.5 nM WR99210 for a week ...
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bioRxiv - Immunology 2024Quote: ... sections were washed with PBS to remove OCT compound on the slides, and blocked with solutions containing imaging buffer (2% FCS, 0.1% Triton X-100 (Sigma, Cat. X100), 0.01% sodium azide) ...
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bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...
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bioRxiv - Cancer Biology 2020Quote: ... All hTERT-HME1-derived cells lines were maintained in DMEM/F12 medium (Pan-Biotech, Germany) supplemented with 10% FCS (Sigma-Aldrich, Germany), 2mM L-glutamine (Pan-Biotech ...
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bioRxiv - Immunology 2020Quote: Cell lines were grown at 37°C in either RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich, UK), 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Emulsions were formed by flow-focusing the collagen/cell solution and oil (fluorocarbon oil FC-40 (Sigma Aldrich, St. Louis, MO, USA)) with 2% FluoroSurfactant (RAN Biotechnologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Serum was collected from groups of mice and an in–house assay was used in which anti–human IgG Fc (Sigma, Catalog # FAB3700259) was used to capture human IgG (MS-Hu6) ...
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bioRxiv - Microbiology 2022Quote: ... At 1 h post infection (hpi) the infection medium was exchanged with DMEM/10% FCS containing 100 µg/ml gentamicin (GEN, Sigma-Aldrich G1397) and increasing concentrations of CIP (0 ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed brain samples were placed securely one at a time in a custom-made MR-compatible holder and immersed in proton-free susceptibility matching fluid (FluorinertTM FC-70; Sigma-Aldrich, UK). Samples were scanned in a random order ...
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bioRxiv - Cell Biology 2020Quote: ... 24h post-plating the cardiomyocytes were fed with fresh medium supplemented with 2% FCS and with 10-5 M Isoproterenol (Sigma I-2760). To induce proliferation ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% heat inactivated fetal calf serum (FCS; PAA, Cölbe, Germany) and 50 U/ml penicillin plus 5 μg/ml streptomycin (Sigma-Aldrich, Munich, Germany). Peripheral blood mononuclear cell (PBMCs ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]), before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2022Quote: ... 25 µL 5% FC-40 oil (RAN Biotechnologies, CN 008-FluoroSurfactant-5wtF) was added to the bottom and 50 µL mineral oil (Sigma, CN M5904) was added on the top ...
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bioRxiv - Neuroscience 2022Quote: ... containing 10 % fetal calf serum (FCS) as well as Pen/Strep (50 U/ml penicillin + 50 μg/ml streptomycin; Sigma-Aldrich, USA). After centrifugation at 900 g ...
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bioRxiv - Immunology 2022Quote: ... and transferred in the digestion medium (HBSS with Ca2+ Mg2+ - Fetal Calf serum (FCS) 2% - DNase I 0.1mg/mL (Sigma-Aldrich, ref 11284932001) – Collagenase IV 0.5mg/mL (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to S1 and RBD antigen was detected using a three-step approach consisting of anti-mouse or antirat Fc biotin (Sigma Aldrich B7401) followed by streptavidin-HRPO (Life Technologies cat# SA1007 ...
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bioRxiv - Immunology 2023Quote: ... CD44-FUT7-Fc collected from cell culture medium was concentrated 200-fold using Amicon® Ultra-15 centrifugal filter units (Millipore Sigma), and 10µl of this concentrate was added to block the HUVEC substrate for 1h before performing the rolling assay at 1dyn/cm2 ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Immunology 2023Quote: ... 110-HG) in the presence or absence of 1 μg mCD152/Fc (CTLA4-Ig, mouse IgG2a, non-cytolytic) chimera (Sigma-Aldrich, C4358). The anti-human IgG Fc Antibody (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... with 10% (v/v) Fetal Calf Serum (FCS, First Link, UK) and 1% (v/v) 10 mM L-Glutamine (Sigma-Aldrich, UK). Trypsin with 1 mM ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Immunology 2023Quote: ... bone marrow from C57BL/6J mice was treated with ACK and cultured in DC medium (RPMI-1640 [Thermo Fisher Scientific], 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) with GM-CSF (20 ng/ml ...
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bioRxiv - Microbiology 2024Quote: ... the infected cells were washed twice and further incubated in DMEM/10% FCS containing 100 μg/ml gentamicin (GEN, Sigma-Aldrich G1397) to remove extracellular bacteria ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet obtained after Percoll layering was plated in non-precoated culture ware for adherent cells and expanded for 4 days in DMEM/F-12 media with 10% FCS (Sigma, St Louis, MO). Microglia were isolated using anti-Cd11b beads (130-049-601 ...
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bioRxiv - Cancer Biology 2019Quote: ... Gartner’s medium consisted of alpha-MEM (BioWhittaker) supplemented with 12.5% heat-inactivated FCS and 12.5% heat-inactivated Horse serum (Sigma-Aldrich, Saint Louis, MO, USA), 1 μM hydrocortisone (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... dispersed cells were cultured for 4 h in RPMI/10% fetal calf serum (FCS) with 1.5 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (all from Sigma-Aldrich, St Louis, Mo) with Golgi-Stop (BD Bioscience ...