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Citations for Millipore Sigma :
451 - 500 of 820 citations for HVEM Rhesus Macaque HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... adherent human embryonic kidney (HEK) 293T cells were transfected with the expression plasmids with branched polyehtyleneimine (PEI, Sigma-Aldrich). In detail ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK 293T cells were transfected with a 1:3 ratio of DNA:polyethylenimine (PEI, 25 kDa linear, Sigma-Aldrich). Transfection of siRNA was performed using X-tremeGENE siRNA Transfection Reagent (Roche ...
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bioRxiv - Biophysics 2022Quote: ... The plasmids were incorporated into pcDNA3.1(+) vectors and were transiently transfected into HEK 293T cells using PEI (Milipore Sigma) as previously described 32 ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T-hACE2-TMPRSS2 cells (called herein HEK-ACE2-TMPRSS2) were induced for TPMRSS2 expression by addition of doxycycline (0.5 μg/mL, Sigma) and were maintained in DMEMc with blasticidin (10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: The Env expression plasmids were used to transfect HEK-293T cells with X-tremeGENE HP DNA Transfection Reagent (Sigma) in combination with either a Tat expression plasmid pTat for Env expression and fusion assays ...
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bioRxiv - Molecular Biology 2021Quote: The lysates of HEK-293T cells transfected with circPTPN12 plasmid were incubated with Protein-A/G agarose beads (Millipore) and antibody against Ago2 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney (HEK) 293T cells and chicken DF-1 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... human embryonic kidney (HEK) 293T cells and chicken DF-1 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma) supplemented with 10% FBS and 100 U/ml Penicillin-Streptomycin (complete DMEM) ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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bioRxiv - Biophysics 2022Quote: Human embryonic kidney cell line (HEK-293T) were seeded on 25 mm No.1.5 H cover glass (Marienfeld) coated with poly-L-lysine (Sigma). 0.15 million cells were maintained in 2ml Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Cell Biology 2023Quote: ... and human embryonic kidney (HEK) 293T cells authenticated by RIKEN were cultured in Dulbecco’s modified Eagle’s medium (DMEM; D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... ACER3-wt or ACER3-E33G expression was induced in FreeStyle 293-F(TetR) cells with 1 µg/mL doxycycline (Sigma). Induced cells were counted and resuspended at 0.5×106 cells/mL in medium containing vehicle (0.0125% DMSO ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were produced by transfection of 293-6E cells (National Research Council Canada) using branched polyethylenimine (PEI) 25kDa (Sigma-Aldrich). Cells were maintained at 37°C and 6% CO2 FreeStyle 293 Expression Medium (Thermo Fisher ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was carried out in freshly made blocking buffer (5% FCS, Sigma-Aldrich, Gillingham ...
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bioRxiv - Immunology 2022Quote: ... in 10 ml VLE DMEM (Biochrom) containing 10% heat-inactivated FCS (Sigma-Aldrich), 0.1% 2-mercaptoethanol (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Spleens were gently meshed in FACs buffer (PBS containing 3% FCS from Sigma) using a 40μM cell strainer to prepare single cell suspensions ...
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bioRxiv - Molecular Biology 2019Quote: ... MDCKII cells were grown in DMEM + 10%FCS + Antibiotic/Antimycotic solution (Sigma A5955)(Williams et al. ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 10% FCS tested to be dox-free (Sigma-Aldrich, Taufkirchen, Germany) and penicillin and streptomycin (final concentrations 100 units/ml and 100µg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
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bioRxiv - Plant Biology 2022Quote: ... and anti-Mouse IgG (Fc-specific)-Peroxydase (Sigma, dilution: 1/10000, 1.5hr incubation). Anti-Hsp90 (Agrisera ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in which the plate was coated with anti–human Fc (Sigma, Catalog # FAB3700259), and after overnight incubation at 4°C ...
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bioRxiv - Immunology 2020Quote: ... the media was replaced by FCS-free medium containing 0.1% BSA (Sigma-Aldrich) and cells were stimulated with 100 μl milk EVs or EV-depleted controls in the presence or absence of 5 μg/ml Poly I:C (Invivogen) ...
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bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO, USA), 10mM HEPES ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected in each matrix 25 μg of Recombinant Sema3C-Fc (Sigma-Aldrich) in a concentration of 50 μg/ml in PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... cell media was supplemented with 500 μg/mL 5-FC (Sigma, MO, USA). For control conditions ...
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bioRxiv - Immunology 2019Quote: ... before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich], 90% FCS [Sigma-Aldrich]). This method preserves immune cell viability ...
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bioRxiv - Immunology 2019Quote: ... before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich], 90% FCS [Sigma-Aldrich]). This method preserves immune cell viability ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Biotinylated goat anti-mouse IgG (diluted 1:500 in 10% FCS) (Sigma-Aldrich) was added to the wells and incubated for 1 h at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... The plasmids were transiently transfected to the TMEM16F-KO HEK 293T cells by using X-tremeGENE9 transfection reagent (Millipore-Sigma). Cells grown on poly-L-lysine (PLL ...
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bioRxiv - Neuroscience 2021Quote: ... 1mL of HEK 293F cells from each batch was mixed together in a 12-well Corning Costar Not Treated Plate (Millipore) and incubated at 37°C with shaking on Orbi-Shaker (Benchmark ...
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bioRxiv - Biophysics 2020Quote: ... The plasmids were transiently transfected to the TMEM16F-KO HEK 293T cells by using X-tremeGENE9 transfection reagent (Millipore-Sigma). Cells grown on poly-L-lysine (PLL ...
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bioRxiv - Molecular Biology 2022Quote: Mammalian lentiviral particles harboring sgRNA-encoding plasmids or cDNA-encoding plasmids were co-transfected with the psPAX2 envelope and VSV-G packaging plasmids into actively growing HEK-293T cells (ATCC) using Xtremegene-HP (Sigma) transfection reagent as previously described (Sarbassov et al. ...
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bioRxiv - Molecular Biology 2023Quote: Lentivirus was produced in HEK 293T cells by jetPEI transfection (Polyplus Transfection, Illkirch, France) or X-tremeGENE 9 DNA Transfection Reagent (Sigma) of a pLenti ...
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bioRxiv - Cell Biology 2023Quote: ... MEFs cells were infected with the filtered lentiviral supernatant obtained from transfected HEK-293T cells and were subsequently selected using Puromycin (Sigma) at 3 μg/ml.
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bioRxiv - Molecular Biology 2023Quote: ... TOE1 was depleted in HEK 293T-REx-derived TOE1-degron cells12 by incubation with 600 μM auxin hormone Indole-3-Acetic Acid (IAA, Sigma) for 8 hours ...
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bioRxiv - Genomics 2023Quote: ... quenched with 10 mL selection media (HEK media as above with 1 µg/mL doxycycline, 100 µg/mL blasticidin (Sigma), and 10nM AP1903 (MedChemExpress)) ...
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bioRxiv - Biophysics 2024Quote: The tsA-201 cells (a variant of human embryonic kidney HEK cells) were obtained from Sigma-Aldrich (St. Louis, MO). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses were packaged by co-transfection of Gag-Pol expressing 293 T cells with expression constructs and envelope vectors (VSV-G) using polyethylenimine (PEI; Sigma-Aldrich). Following transduction with Ccl2 or control Empty constructs ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses were packaged by co-transfection of Gag-Pol expressing 293 T cells with expression constructs and envelope vectors (VSV-G) using polyethylenimine (PEI; Sigma-Aldrich). Following transduction with shRNA retroviral constructs ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected into 293T cells at a 1:1:1:1.6:4.6 ratio using 293 Free transfection reagent (EMD Millipore Cat #72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were co-transfected into 293T cells at a 1:1:1:1.63:4.63 ratio using 293 Free transfection reagent (EMD Millipore Cat# 72181) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and co-transfection of heavy and light chain expression plasmids into 293-6E cells (National Research Council of Canada) using 25 kDa branched polyethylenimine (Sigma-Aldrich). After 6-7 days at 37°C and 6% CO2 under continuous shaking in FreeStyle Expression Medium supplemented with 20 U/ml penicillin and 20 µg/ml streptomycin (all Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich) were used.
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bioRxiv - Molecular Biology 2020Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma Aldrich) were used.
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in CF media + 37.5mg/ml 70 kDa Ficoll (Fc; Sigma, F2878) and 25 mg/ml 400 kDa Ficoll PM (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Selective oxidation of hmC to fC was achieved using potassium perruthenate (KRuO4, Sigma-Aldrich) as described (Booth et al. ...
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bioRxiv - Neuroscience 2020Quote: ... 5% FCS with primary antibodies: anti-Calbindin D-28K (rabbit polyclonal, 1:1000, Millipore, AB1778 ...