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Citations for Millipore Sigma :
751 - 800 of 886 citations for Ebola Virus Like Particles since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Chromatographic separation (further desalting) was performed on a Discovery® BIO Wide Pore C5 column (100 × 2.1 mm, particle size 3 μm, Sigma-Aldrich) at 55 °C with a mobile-phase system consisting of water and acetonitrile (each containing 0.1% formic acid) ...
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bioRxiv - Microbiology 2020Quote: ... were incubated with AdV-C5 (moi ∼ 54400 or 13600 virus particles per cell) at 37°C for 60 min in DMEM supplemented with 0.2% bovine serum albumin (BSA, Sigma-Aldrich, A9418) and penicillin-streptomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000073737 5’-CCGGCGCGTTATCAACTGGATCCAACTCGAGTTGGATCCAGTTGATAACGCGTTTTTG-3’ designed and cloned into the lentiviral pLKO.1 puromycin resistant vector Mission shRNA lentiviral Transduction particle (Sigma Aldrich). Control Caco2 clones (shNT ...
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bioRxiv - Microbiology 2021Quote: ... Spore suspensions were then incubated for 1□h with 1:20 donkey anti-mouse IgG antibody coupled to 12-nm gold particles (Sigma G7777) in PBS–1% BSA for 1□hr at RT ...
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bioRxiv - Biochemistry 2020Quote: ... HCT 116 cell pools stably transduced with the WRNr transgene carrying lentivirus particles were selected with 2 μg/ml of Puromycin (SIGMA, P9620) added to the normal growth medium ...
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bioRxiv - Molecular Biology 2022Quote: ... without or with appropriate concentrations of MgCl2 were mixed with a suspension of 10 nm fiduciary gold particles (Sigma Aldrich # 741957), which were coated in bovine serum albumin to prevent clustering ...
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bioRxiv - Synthetic Biology 2022Quote: ... equipped with a Discovery HS F5 150 mm × 46 mm column (particle size 5 μm) (Sigma-Aldrich, St. Louis, MO, USA). The column was kept at 30 °C ...
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bioRxiv - Microbiology 2023Quote: ... coupled to a photodiode array detector and equipped with a Discovery HS F5 150 mm x 46 mm column (particle size 5 μm; Sigma-Aldrich). Solvent A was 10 mM ammonium formate [Merck ...
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bioRxiv - Neuroscience 2023Quote: ... Western Blotting experiments on viral particles were carried out on the collected culture media concentrated with Vivaspin 6 MWCO 30 kDa membranes (Sigma Aldrich). Samples were then reduced and denatured by boiling in 4X Laemmli sample buffer at 95°C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... time-course experiments were performed for trypsin where HIV pseudovirus particles were incubated with a final concentration of 100 ug/mL Trypsin-TPCK (Sigma-Aldrich) for 1 ...
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bioRxiv - Biochemistry 2023Quote: ... and processing blank sample were separated on a Millipore SeQuant ZIC-pHILIC (2.1 × 150 mm, 5 µm particle size, Millipore Sigma #150460) column with a ZIC-pHILIC guard column (20 × 2.1 mm ...
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bioRxiv - Bioengineering 2023Quote: ... and 0 µl of concentrated lentiviral particles were reverse transduced into 5×104 HEK293T cells with 8μg/mL polybrene (Millipore-Sigma, TR1003G) in 24 well format with media exchanged after 14 hours of transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... as prescribed by the manufacturer or by infecting cells with lentiviral particles in DMEM supplemented with 10% FBS and 5 µg/ml polybrene (Sigma #H9268) for 48h followed by a 48h-treatment with 2 µg/ml puromycin.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed to remove viral particles and transduced cells were selected in the presence of 1μg/ml puromycin (Sigma-Aldrich, P8833) for at least 96 h.
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bioRxiv - Biochemistry 2023Quote: ... Adherent Expi293 cells were infected with concentrated lentiviral particles at 75% confluency in adherent culture media supplemented with 8 μg/mL polybrene (Sigma-Aldrich), then incubated for 48 h at 37 °C ...
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bioRxiv - Biophysics 2023Quote: The velocity profiles inside the microchannels are obtained by tracking neutrally buoyant red fluorescent particles (Fluro-Max, size: 860 ± 5 nm, procured from Sigma-Aldrich) added to the base fluid in an appropriate amount ...
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bioRxiv - Bioengineering 2023Quote: ... the obtained multilayer Ti3C2Tx MXene particles from the previous step were reacted with 100 mL aqueous solution of LiCl (1.18 M, 99% Sigma Aldrich, Germany) for 24 hours at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... A 2 µL sample was injected into a normal phase Ascentis® Express HILIC UHPLC column (2.1 x 100 mm, 2 µm particle size; Sigma, USA) and eluted at 30°C over a 20-minute gradient with a flow rate of 400 µL/min ...
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bioRxiv - Bioengineering 2024Quote: ... cells were exposed for six hours to virus with an MOI (multiplicity of infection) of 5 viral particles/cell in the presence of 4 μg/mL polybrene (Sigma-Aldrich). Cells were then washed ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse leukemic progenitors were transduced by exposing them to viral particles and room temperature centrifugation at 1000g for 1 hr in 8uG/mL polybrene (Millipore Sigma). Spinfection plates were pre-coated using 500 uL of PBS with 10 uG/mL of purified human plasma fibronectin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... hCEcto stem cells were grown on collagen-coated six well-plate to 50% confluence before they were treated with virus solution and Polybrene (1μg/mL) (Sigma-Aldrich, # H9268) overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... elephas RNAs were reverse transcribed with an oligo dT primer using an enhanced avian myeloblastosis virus reverse transcriptase enzyme (Sigma-Aldrich) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... 6×105 cells were infected in 6-well plates with 125uL of high titer virus in standard media with 5ug/mL Polybrene (Sigma-Aldrich). Media was changed after 24 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... The virus was harvested 48 hours post transfection and filtered through a 0.45 μm PES filter (Merck-Millipore, Burlington, Massachusetts, USA). PM-PDX derived cells were cultured in complete EMEM until a density of approximately 60% was reached ...
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bioRxiv - Cancer Biology 2020Quote: ... Infection of P3 and T98G cells was performed by centrifugation of cells with virus at 2225 rpm for 90 minutes at room temperature in the presence of 10 μg/mL polybrene (Sigma-Aldrich). Enrichment selection using puromycin (1 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Infection of P3 and T98G cells was performed by centrifugation of cells with virus at 2225 rpm for 90 minutes at room temperature in the presence of 10 μg/mL polybrene (Sigma-Aldrich). Enrichment selection using puromycin (1 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 24-48 h with the virus supernatant supplemented with 8 µg ml-1 polybrene (Sigma-Aldrich #H9268) before being subjected to further experimental procedures.
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bioRxiv - Microbiology 2020Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... all transductions were performed in multiple plates with a range of virus volumes and 8 μg mL−1 polybrene (EMD Millipore), but only the pool with the most optimal transduction efficiency was expanded and/or screened.
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bioRxiv - Microbiology 2021Quote: 3 μl of A/PR/8/34 (H1N1) influenza virus mixed with 1 μl of 5x BSA-coated 10 nm colloidal gold (Sigma-Aldrich) were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil) ...
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bioRxiv - Microbiology 2021Quote: A549-ACE2 cells were infected by incubating the virus for 1h with the cells maintained in DMEM supplemented with 1 μg/ml TPCK-trypsin (Sigma #4370285). The viral input was then removed and cells were kept in DMEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2021Quote: Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma−Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... whole-bodies of virus-injected females (DENV and ZIKV-infected) were homogenised with glass beads in Trizol (Sigma-Aldrich, MA, USA) on a Precellys 24 homogeniser (VWR).
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin (Puromycin dihydrochloride ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin ...
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bioRxiv - Cancer Biology 2022Quote: ... P12-ICHIKAWA cells were infected with Lenti-FUW-Luc-mCh-puro virus and selected in liquid culture with puromycin (Sigma-Aldrich) 2.5µg/mL for 7 days following mCherry+ cell sorting.
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bioRxiv - Microbiology 2019Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... were added to each well (around 6 × 105 cells per well) along with a no virus control with 8 µg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight (12-16 hours) ...
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bioRxiv - Synthetic Biology 2021Quote: ... viral transductions were performed overnight in a 6-well dish using 800 µl of standard ESC medium with 200 µl virus-containing supernatant supplemented with 8 µg/ml polybrene (TR-1003; Sigma-Aldrich). The selection of positive clones was performed by supplementing the culture medium with 1µg/ml puromycin for 3 days ...
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Genomics 2021Quote: The titer of lentivirus was determined by transducing 500,000 cells with varying amount (0, 0.5, 1.0, 2.0, 4.0 and 8.0 µL) of concentrated virus and polybrene (Millipore, TR-1003-G, 8 µg/mL,). Viral transduction was performed by centrifuging the lentivirus and cell combination at 1000 RCF for 90 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... and 500 μL) were added to each well along with a no virus control with 7 μg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight ...
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bioRxiv - Immunology 2021Quote: ... On the following day the virus-containing medium was collected and filtered using 0.45 µm filter (Millipore Sigma, Cat. no: SLHVM33RS). Fresh HEK293T cells were infected by adding the lentivirus-containing medium to culture medium followed by 1 to 2 day incubation ...
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bioRxiv - Immunology 2020Quote: ... The swabs were placed into 1 ml of Trizol or 2 ml of virus transport medium comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... A 500 μL aliquot was collected every 10−50 s (depending on the virus) and mixed with 5 μL of 5,000 mg L−1 sodium thiosulfate (Sigma-Aldrich, Germany) to instantly quench the residual free chlorine ...
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bioRxiv - Microbiology 2022Quote: KSHV BAC16 virus stock was prepared by inducing lytic cycle in iSLK-BAC16 cells with Doxycycline (1 µg/ml; Sigma-Aldrich) and sodium butyrate (1 mM ...
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bioRxiv - Neuroscience 2022Quote: ... RabiesΔG-mCherry virus (titer of around 110 TU/ml) was mixed at a 2:1 ratio with silk fibroin (Sigma #5154) to make rabies injection solution (Jackman et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with PBS and virus media with 1 μg/ml TPCK treated trypsin (Sigma-Aldrich, USA) was added ...