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Citations for Millipore Sigma :
551 - 600 of 886 citations for Ebola Virus Like Particles since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The purified viral particles were then concentrated using an ultrafiltration membrane unit (Amicon Ultra; EMD Millipore, Burlington, MA, USA). Virus titers were determined by quantitative PCR using a pair of primers for the WPRE sequence (forward ...
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bioRxiv - Microbiology 2021Quote: ... Viral particles were filtered though a 0.45 μm MF-Millipore Filter (Millex) and centrifuged through a 20% sucrose (Sigma) cushion ...
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bioRxiv - Cell Biology 2021Quote: ... and kinetochores particles were eluted by gentle agitation of beads in elution buffer (Buffer H + 0.5mg/mL 3FLAG Peptide (Sigma)) for 30 min at room temperature.
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bioRxiv - Cell Biology 2020Quote: ... The samples were resolved on the Discovery HS F5-3 column (2.1 mmI.D. x 150 mmL, 3 μm particle, Sigma-Aldrich), using a step gradient with mobile phase A (0.1% formate ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Thiol groups on particles were estimated by Ellman’s reagent (G Biosciences, Cat# BC87) using cysteine (Sigma-Aldrich, Cat# 168149) as the standard ...
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bioRxiv - Immunology 2022Quote: ... Then viral particles were collected and concentrated 100-fold by mixing with PEG 8000 (Sigma-Aldrich, St Louis, MO) for 12 h at 4°C and centrifuging for 30 min at 1600×g at 4°C.
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bioRxiv - Microbiology 2021Quote: ... Microbial cells were detached from soil particles by mixing with sterile 0.2% (w/v) tetrasodium-pyrophosphate decahydrate solution (pH 7.5, Sigma-Aldrich), and then purified by sucrose gradient solution centrifugation as described by (61) ...
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bioRxiv - Microbiology 2020Quote: ... 1 mM DTT) and treated with 3×45 s bead beating with glass beads (particle size≤106 μm Sigma) at maximum power and cooling on ice between the cycles (MP Biomedicals™ FastPrep-24™ ...
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bioRxiv - Immunology 2020Quote: ... The BMDM and BMDCs cells were infected with lentiviral particles for 1day and treated with 1.5 μg/ml puromycin (Sigma) for 2 days ...
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bioRxiv - Immunology 2019Quote: ... Viral particles were added to cultured THP-1 cells in R10 (Roswell Park Memorial Institute medium (RPMI-1640) (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: ... short-hairpin RNA against PRKCI target sequence 5’-AGTACTGTTGGTTCGATTAAACTCGAGTTTAATCGAACCAACAGTACT-3’ was used to generate lentiviral particles (Sigma Mission-TRCN0000219727). Lentiviral particles were generated by transfecting HEK293T cells ...
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bioRxiv - Neuroscience 2021Quote: ... a craniotomy was performed under isoflurane anesthesia and 0.5 μL of lentiviral GPR83 shRNA or control shRNA particles (109; Sigma Mission Lentiviral Transduction Particles ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of clarified lysate was incubated with 30 μl of pre-rinsed streptavidin magnetic particles (11641786001, Sigma-Aldrich) in 1 ml RIPA buffer for 16h ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 μL of the supernatant was injected onto a ZIC-pHILIC 150 × 2.1mm (5μm particle size) column (EMD Millipore). Chromatographic separation was achieved using the following conditions Mobile phase A consisted of 20 mM ammonium carbonate with 0.1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were separated on a Discovery HS F5-3 column (2.1 mm i.d. × 150 mm long, 3 µm particle size, Sigma–Aldrich) at a flow rate of 0.25 ml min−1 with a step gradient of mobile phase A (0.1% formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... After 24 and 48 h the supernatants containing viral particles were collected and filtered using a 0.22 µm syringe filter (Millipore).
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bioRxiv - Neuroscience 2023Quote: ... samples were reconstituted in 50 uL water and 2 uL were injected onto a SeQuant® ZIC®-pHILIC 150 x 2.1 mm analytical column equipped with a 2.1 x 20 mm guard column (both 5 mm particle size; Millipore-Sigma). Buffer A was 20 mM ammonium carbonate ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant containing the virus particles were added to MDCKII WT and KO cells after being filtered through a sterile 0.45 µm filter (Millipore). Here ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA from B.1 viral particles was isolated from Vero cell supernatant and digested by P1 nuclease (Sigma, N8630) followed by treatment with phosphatase (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Transfected cells were grown for 72 hr and then the supernatant containing the viral particles was collected and passed through a sterile 0.45 µm Millex syringe filter (Millipore). HEK293 FLAG-Cas9 cells (a gift from Alice Ting ...
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bioRxiv - Cell Biology 2023Quote: ... The collected viral particles were then cleaned and concentrated using Amicon 100K (100kDa) cut-off columns (Sigma Aldrich, USA). AAV-GFP was used as a control.
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bioRxiv - Cell Biology 2023Quote: ... 2 μL of this resuspended sample were injected onto a SeQuant® ZIC®-pHILIC 150 × 2.1 mm analytical column equipped with a 2.1 × 20 mm guard column (both 5 mm particle size; Millipore Sigma). Buffer A was 20 mM ammonium carbonate ...
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bioRxiv - Cancer Biology 2023Quote: ... sub-confluent target cells were incubated with particles and 4 µg/mL polybrene (Hexadimethrine bromide; Sigma-Aldrich, Lyon, France) for 18 h ...
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Safe plant Hsp90 adjuvants elicit an effective immune response against SARS-CoV2 derived RBD antigenbioRxiv - Molecular Biology 2023Quote: ... after which the supernatant containing pseudo-typed lentivirus particles was harvested and filtered through a 0.45-µm filter (Millipore). The viral particles were concentrated by centrifugation at 3000 x g overnight at 4°C and the pellet was resuspended in DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were separated on a Discovery HS F5-3 column (2.1 mm i.d. × 150 mm, particle size 3 μm, Sigma-Aldrich) using mobile phase A (0.1% formic acid/water ...
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bioRxiv - Genetics 2024Quote: ... Two microliters of sample were injected into a ZIC-pHILIC 150 x 2.1 mm (5μm particle size) column (EMD Millipore). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Plant Biology 2024Quote: ... The instrument was fitted with a Supelco TitanTM C18 UHPLC Column (80Å, 100 x 2.1 mm, particle size 1.9 μm; Sigma Aldrich). Injections of 2 μL were separated by a solvent gradient consisting of mobile phase A (water + 0.1% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... 293 [HEK-293] (ATCC® CRL-1573TM) stable cell lines were generated via transduction of lentiviral particles and selection with 2.5 μg/ml puromycin (Sigma) for 4 days ...
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bioRxiv - Immunology 2024Quote: ... Early passage BEAS-2B.Cas9 cells were then transduced with supernatant containing these lentiviral particles in the presence of 200 μg Polybrene (Sigma). Transduced cells were selected by culturing with 8 μg/ml Puromycin (Sigma ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Target cells were then transduced overnight with the filtered virus in the presence of 8 μg/mL polybrene (Millipore).
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Cell Biology 2022Quote: ... 293T or 293GP virus producing cells were transfected using the GeneJuice reagent according to the manufacturer’s recommendations (Merck Millipore). Two days after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.1 to 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(- ...
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bioRxiv - Microbiology 2021Quote: ... Virus-containing flocs were then recovered from the sample by filtration onto 90mm 0.2um polycarbonate filters (Isopore, GTTP09030, Millipore) under gentle vacuum ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed and inoculated with virus diluted in infection media (DMEM with 0.3% BSA and 1μg/ml TPCK-treated trypsin (Sigma Aldrich)) and allowed to adsorb for one hour ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT) for 2 hours at 30°C with native Tobacco Etch Virus (TEV) protease (Sigma-Aldrich). The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... A 2 mL volume of warm virus medium (1X MEM with 100U/100 μg/mL penicillin/streptomycin, 0.3% bovine serum albumin [Sigma]) was added to each well ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Each cell line was infected with varying volumes of concentrated virus in the presence of polybrene (Sigma Aldrich 107689) to determine the concentration that conferred survival in puromycin to 30% of cells ...
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bioRxiv - Cell Biology 2021Quote: ... The target cell lines at 50-60% confluency were then infected using 3 mL virus with polybrene reagent (Sigma). After 24 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... except that each virus was added to SVOG3e/rtTA cells and selected with 0.5 μg/ml Puromycin (Sigma P8833) and 0.6 mg/ml Geneticin to create polyclonal stable cell lines SVOG3e/rtTA/V5 (V5 control cell line) ...
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bioRxiv - Cancer Biology 2020Quote: ... Viral mixes were created with an aliquot of virus into complete media and polybrene at 8μg/ml (Sigma-Aldrich) to have a target multiplicity of infection (MOI ...
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bioRxiv - Immunology 2019Quote: Media was recovered approximately 48-hr post-transfection and cell-free virus stocks were generated by filtering the media through 0.45 µM filters (Millipore). The TZM-bl indicator cell line was used to quantify the amount of infectious virus(Derdeyn et al. ...
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bioRxiv - Biochemistry 2019Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using Moloney Murine Leukemia Virus Reverse Transcriptase (Sigma) and oligo dT (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... The activated cells were added to the virus-bound plates after removing dead cells using Histopaque-1119 (Sigma-Aldrich), and centrifuged at 3200 × g for 90 min at 32°C ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lentivirally transduced by spinfection with centrifugation of cells with virus in the presence of 8μg/ml polybrene (Millipore) at 1,000xg for two hrs ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were seeded onto glass-bottom dishes and transduced with virus containing pEGFP_EB3 (gift from S. Godinho) Cells were treated with Eg5 (Kif11) inhibitor monastrol (67 μM, Sigma) for 2 hr ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RAMP1 expression achieved through transduction of virus containing RAMP1 MISSION TRC3 Open Reading Frame (ORF) plasmid (pLX_304) (Sigma, US) into RAMP2 KO-HUVECs ...
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bioRxiv - Synthetic Biology 2020Quote: ... the virus was removed and the cultures overlaid with a viscous solution of 1% (w/v) carboxymethylcellulose (Sigma #C4888) in DMEM-F12 (8% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... virus was mixed with 0.36 million cells in growth media supplemented with 8 μg/mL Polybrene (Sigma-Aldrich, H9268) and incubated overnight ...