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Citations for Millipore Sigma :
7901 - 7950 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The harvested protein was concentrated by Amicon Ultra-15 centrifugal filter units (Millipore Sigma, Burlington, MA, USA), filtered through a 0.22 µm filter (E&K Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were incubated overnight with 5 µL of Flag or V5 antibody-conjugated beads (Sigma-Aldrich) or with 1 µl of p50 antibody together with 20 µl of Protein A magnetic beads (Life Technologies) ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were separated by SDS-PAGE and then transferred to a PVDF membrane (Millipore, Burlington, Massachusetts). Membranes were stained with 1:1000 dilution of Anti-SARS-CoV SΔ10 antibody ([1A9] ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were run in SDS-PAGE and transferred to Immobilon Polyscreen PVDF transfer membranes (#IPVH00010, Merck Millipore) or Amersham Protan nitrocellulose membranes (#10600001 GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... and proteins were eluted in 50 mM Tris-HCl (pH 8) containing 10 mM reduced glutathione (Sigma). Glutathione was removed using a desalting Zeba column following manufacturer instructions (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: Immunodepletion of Cyclin A from Xenopus embryo extracts was carried out using AffiPrep Protein A beads (Sigma) conjugated with the Xenopus laevis anti-Cyclin A1 (a gift from Daniel Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... After eluting and concentrating the cleaved protein in Amicon Ultra-15 Centrifugal Filters 50K MWCO (Merck Millipore), size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2020Quote: The indicated proteins were loaded to SDS-PAGE gel and transferred to methanol-activated PVDF membranes (Millipore), which were blocked in 5% non-fat milk containing 0.2% Tween for 1 h at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of both protein constructs was similar: plasmid was transformed into the Rosetta2 strain of E.coli (Novagen). Two liters of culture were grown in terrific broth for 8-12h at 37°C (until the OD600 ~2) ...
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bioRxiv - Cell Biology 2019Quote: ... Aurora B (0.01 µg/µl) and myelin basic protein (MBP; 2.5 µg) (Sigma-Aldrich and Biaffin GmbH) or one of the three Mid1p domains (“N-term” ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we employed antibodies against RNA-Binding Protein RBFOX3 (aka NeuN; mouse anti-NeuN, Alexa488-conjugated, MAB377X, Millipore) that is expressed in all neuronal nuclei ...
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bioRxiv - Genetics 2021Quote: Total protein was isolated using RIPA lysis buffer (Biocolors, China) with a protease inhibitor cocktail (Sigma, USA). All protein samples were subjected to concentration determination and immunoblotting assay with the indicated antibodies ...
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bioRxiv - Microbiology 2021Quote: ... for 5h and then Protein G-DAF complexes were crosslinked using bis(sulfosuccinimidyl)suberate (BS3) (Sigma, S5799). Protein from total cell extracts (100 µg ...
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bioRxiv - Immunology 2020Quote: ... coli BL21(DE3) strain and protein expression was induced in Overnight Express(tm) Instant TB Medium (Novagen) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... This magnetic resin was generated by incubating Dynabead Protein G (ThermoScientific) with anti-FLAG antibodies (Sigma Aldrich) at room temperature followed by washing ...
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bioRxiv - Immunology 2020Quote: ... Detection of the SARS CoV-2 spike protein was achieved by incubation with an anti-rabbit (Sigma) secondary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... (Table 2) and the immune complexes were captured with protein A agarose fast-flow beads (Sigma-Aldrich) for 1h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Proteins contained in BALF and TS were concentrated 5x using an Amicon Ultra column 100kDa (Merck Millipore) and 20 min centrifugation at 4000xg and samples were stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... The precipitated protein and input control were detected by the Western blotting with anti-Flag (Sigma, A8592) or anti-GFP (Abcam ...
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bioRxiv - Immunology 2021Quote: ... the protein elution fractions were pooled and concentrated with 50 kDa molecular weight cutoff ultrafiltration unit (Millipore), then 1 mg of TEV protease was used per 50 mg protein forcleavage during dialyzing to the buffer with 250 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: The fractions representing the monomeric protein were collected and concentrated with Amicon Ultra-centrifugal filters-50K (Millipore). The quality of the final protein product was examined by SDS PAGE ...
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bioRxiv - Physiology 2020Quote: ... The targeted protein bands were visualized with extended chemiluminescent (ECL) reaction using Immobilon western HRP substrate (Millipore) or by the VECTASTAIN ABC Kit (VECTOR Laboratories ...
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bioRxiv - Genomics 2020Quote: ... the immunocomplexes were collected with 25 μL of PureProteome™ Protein A Magnetic (PAM) Beads (Millipore, LSKMAGA10) for 2 hours at 4°C with rotation.
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bioRxiv - Systems Biology 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
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bioRxiv - Cell Biology 2021Quote: ... the solution was incubated with PureProteome™ Protein G Magnetic Beads (Cat. # LSKMAGG10, Millipore, Burlington, MA, USA) and washed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the proteins bound to the beads were eluted by the addition of FLAG peptide (#F3290, Sigma-Aldrich). Proteins in the eluate were separated by SDS-PAGE to detect Tanmp-interacting proteins by Western blotting or by silver staining ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble chromatin was precleared with Magna ChIP™ Protein A+G Magnetic Beads (Millipore, 16-663) for 1h and then incubated overnight at 4°C with magnetic FLAG-M2 beads ...
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bioRxiv - Microbiology 2022Quote: ... followed by nucleic acid digestion with 0.5 Units of Benzonase per 1 µg of protein (Sigma-Aldrich) for 30 min at 37°C ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein was washed and concentrated using 30 kDa Amicon centrifugal filter unit (Millipore Sigma CAT#UFC8030).
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bioRxiv - Molecular Biology 2022Quote: ... β-mercapto-ethanol 2.8 mM) in presence of thrombin (50 units/10 mg of purified protein; Sigma). The resulting digest was loaded into an 5ml HisTrap column previously equilibrated in buffer B and the flow through was collected ...
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bioRxiv - Biochemistry 2022Quote: Protein extracts were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Merck, Germany). Membranes were blocked with 5% non-fat milk and incubated with a specific anti-biotin antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitated proteins were eluted with 0.5 mg/mL (diluted in lysis buffer) 3X FLAG peptide (Sigma, F4799) for 30 minutes on a rotator ...
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bioRxiv - Biochemistry 2022Quote: Biotinylated proteins were digested with 250 ng of trypsin mass spectrometry grade (Sigma-Aldrich, St. Louis, MO) in 50 mM of ammonium bicarbonate (ABC) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to nitrocellulose membranes before blocking with 5% milk (Marvel) or bovine serum albumin (Sigma) w/v in PBS-T (0.1% Tween-20 in PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... bound proteins were eluted with lysis buffer containing 300 μg/ml Flag peptide (Sigma-Aldrich, Cat# F3290) at 4 °C for 45 min ...
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bioRxiv - Bioengineering 2022Quote: ... supernatant protein was removed by acidification with 5 g L-1 trichloroacetic acid (Supelco from Sigma-Aldrich), incubation for 20 min in room temperature and centrifugation at 20,238 x g for 10 min in a table-top centrifuge (Eppendorf 5424 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to approximately 3 mg/ml using an ultrafiltration pressure cell (Millipore, Billerica, MA) with a 10-kDa molecular mass cutoff membrane and buffer exchanged with 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purified protein were concentrated using a 0.5 ml Amicon Ultra centrifugal filter (Merck Millipore) with a membrane molecular weight cut off 10 kDa.
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bioRxiv - Biochemistry 2022Quote: ... Protein-containing fractions were pooled and concentrated using centrifugal ultra-filtration devices (10 kDa cut-off; Millipore). Protein concentration was determined using a Bradford assay with a standard curve calibration with BSA ...
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bioRxiv - Biophysics 2022Quote: ... the expression of the protein was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG – Sigma) and the flask was placed at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was reduced with a final concentration of 10 mM Tris(2 - carboxyethyl)phosphine hydrochloride (TCEP) (Sigma) and alkylated with 40 mM 2-chloroacetamide (CAA ...
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bioRxiv - Cell Biology 2022Quote: ... LATS1 kinase was immunoprecipitated from 500 µg of protein lysates and subject to Oxyblot assay (#S7150, Millipore) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Immunodetection of ProteinA (ProtA)-tagged proteins was performed using Peroxidase Anti-Peroxidase Soluble Complex antibody (Sigma-Aldrich) in a 1:3,000 dilution ...
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bioRxiv - Developmental Biology 2022Quote: Separated proteins were wet transferred onto 0.45mm pore-sized PVDF membranes (Immobilon®-FL PVDF membrane, Millipore) for 2 hours at 250mA and 4°C using cold transfer buffer (1.5x NuPAGE transfer buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The presence of proteins in samples was detected using Immobilon Western Chemiluminescent HRP Substrate (Sigma-Aldrich, France). Acquisition was performed on a ChemiDoc Imaging System (Biorad ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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bioRxiv - Immunology 2022Quote: ... Equivalent amounts of protein were separated by SDS-PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Membranes were incubated with antibodies per manufacturer’s suggestions ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003; Sigma-Aldrich/Merck) on a 4-20% precast Trupage gel (#PCG2012-10EA ...