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Citations for Millipore Sigma :
8151 - 8200 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Proteins were electrophoretically separated and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Merck Millipore, Burlington, MA, USA). Blocking treatment was performed using the PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein levels of wild-type or mutant Rad6 were determined by immunoblotting using anti-Flag M2 antibody (Sigma).
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bioRxiv - Physiology 2019Quote: ... The silver stained protein spots were de-stained using the ProteoSilver Plus Stain Kit (Sigma-Aldrich, MO, USA), and in-gel enzymatic digestion was done overnight at 37°C using Trypsin Singles ...
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bioRxiv - Physiology 2019Quote: ... Gels containing protein spots were then stained using the ProteoSilver Plus Silver Stain Kit (Sigma-Aldrich, MO, USA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and 0.1% NP-40 substitute (Fluka)) and added to 5-7 µL of protein A sepharose beads (Sigma) in a 50% slurry from the vendor that had been washed in IPP150 and conjugated to ∼5-10 µg of affinity-purified or protein A-purified anti-Prp43p ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were separated using SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-FL, EMD Millipore). Western blotting was performed using rabbit-anti-SRGAP2 N-terminal (raised against residues 193-205 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified protein was buffer exchanged into TBS using a 10,000 Da molecular weight cut-off filter (Millipore-Sigma) through 3 successive washes ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified protein was buffer exchanged into TBS using a 10,000 Da molecular weight cut-off filter (Millipore-Sigma) through 3 successive washes ...
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bioRxiv - Molecular Biology 2020Quote: ... Incubated RdRp complex protein was concentrated with a 100 kDa molecular weight cut-off Centrifugal Filter (Millipore Corporation) and then size-separated by a Superdex S200 10/300 GL column in 25 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA-cross-linked proteins were immunoprecipitated (1% kept as input) using 5 μg of NF-kB/p65 (Millipore) or control mouse IgG antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein extracts were then loaded on 10% SDS-page acrylamide gels and transferred on PVDF membrane (Millipore #IPVH00010) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were eluted by adding 125 ng/ml FLAG peptide to the OG-containing buffer (Sigma-Aldrich) and incubating the beads for 30 min per round of elution ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then trypsinised by incubating with 5 µg/ml trypsin in 25 mM triethylammonium bicarbonate (Sigma-Aldrich) overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... then resuspended in 2ml papain solution [0.75% v/v of papain (25mg/ml, 17U/mg protein, Sigma-Aldrich), 40μg/ml DNase I type IV ...
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bioRxiv - Microbiology 2019Quote: ... The eluted protein complex was concentrated using a 100 kD MWCO Amicon Ultra Centrifugal Filter Unit (EMD Millipore). The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences ...
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bioRxiv - Microbiology 2019Quote: ... The eluted LytH protein was concentrated using a 50 kD MWCO Amicon Ultra Centrifugal Filter Unit (EMD Millipore). The LytH protein was further purified using size-exclusion chromatography with a Superdex 200 Increase 10/300 GL column (GE Life Sciences ...
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bioRxiv - Neuroscience 2019Quote: ... 700-1000 µg total protein was incubated overnight at 4°C with 1:100 anti-FLAG (Sigma, F1804) or anti-Myc (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were denatured and then separated on SDS-PAGE and transferred to PVDF membrane (Millipore, Burlington, USA). After blocking by non-fat milk for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the protein were pooled and concentrated using an Amicon with a 3 KDa cut-off (Millipore). The protein was conserved in 10 mM Tris-HCl pH7.63 and 33% glycerol ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were concentrated using Amicon Ultra centrifugal filter devices (30 kDa molecular weight cut-off, EMD Millipore), and were quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2, Millipore Sigma #04-263) at a dilution of 1:400 for 3 hours at room temperature.
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A key interfacial residue identified with in-cell structure characterization of a class A GPCR dimerbioRxiv - Biochemistry 2019Quote: Protein samples were separated on a 10% SDS-PAGE and transferred to nitrocellulose membranes (Millipore Corporation, Billerica, USA). The non-denaturing condition was achieved with the use of SDS-free sample buffer and without sample boiling before loading sample ...
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bioRxiv - Plant Biology 2019Quote: ... StrepII-tagged proteins were detected using Strep-Tactin AP conjugate (IBA) or a mouse monoclonal StrepII antibody (Sigma). Further primary antibodies used were α-mCherry (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... Lamin proteins were detected using mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR) ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant (total protein concentration ∼3 mg/ml) was incubated with ANTI-FLAG M2 agarose beads (Sigma Aldrich) at 4°C for 1 h with agitation ...
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bioRxiv - Genetics 2021Quote: ... Complexes were diluted to 2.5uM in a protein storage buffer composed of 50% (v/v) glycerol (Sigma G5516), 100 mM NaCl (Fisher Scientific S271-3) ...
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bioRxiv - Genomics 2020Quote: ... Approximately 10 mg of total protein was added to 30 μl anti-FLAG M2 magnetic beads (Sigma # M8823) prepared according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... anti-parvalbumin (PV;P3088) and anti-glial fibrillary acidic protein (GFAP;G3893) antibodies were obtained from Sigma-Aldrich; bromodeoxyuridine (BrdU ...
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bioRxiv - Molecular Biology 2021Quote: ... The elution of PAN-protein complexes was performed using 0.5 μl Benzonase nuclease (125U final, EDM Millipore 71206) in 1 ml of 0.35 M NaCl for 2 h at 37 °C (45 ...
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bioRxiv - Microbiology 2021Quote: ... MERS S-protein was detected using a monoclonal anti-FLAG M2-HRP conjugated antibody (SIGMA, A8592 @ 1:2000) which bound to a C-terminal FLAG-tag ...
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bioRxiv - Microbiology 2021Quote: ... Equal protein amount were used to perform SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF) (Millipore, USA) by the semi-dry transfer (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were extracted using RIPA buffer with protease inhibitors (cOmplete™, EDTA-free Protease Inhibitor Cocktail; Millipore Sigma). Sample input was normalized using a Bradford protein assay that was calibrated with BSA standards ...
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bioRxiv - Cell Biology 2021Quote: ... Expression of EGFP fusion proteins was then induced by addition of 1 µg/mL doxycycline (Sigma-Aldrich, D9891) and EGFP expressing cells were positively selected by FACS ...
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bioRxiv - Biochemistry 2021Quote: 100 bacterial homologs of nucleobase/cation symporter 2 (NCS2) proteins were cloned into a modified pET28 plasmid (Novagen) with either an N- or a C-terminal deca-histidine tag and a TEV protease recognition site at the central facility of the New York Consortium on Membrane Protein Structure (NYCOMPS ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was treated by 0.5 mg/mL drICE protease and incubated with 2 mM NADPH (Sigma-Aldrich) for 2 h at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Fractionation of the nuclear protein was performed according to the Pierce cytoplasmic and nuclear extraction kit instructions (Sigma). Samples were run on an SDS-Page gel (BioRad ...
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bioRxiv - Bioengineering 2020Quote: ... Protein was covalently bound to the slide surface via addition of freshly prepared 2.5 mg/mL NaBH4 (Sigma) dissolved in 25 %v/v ethanol in PBS at RT for 5 min ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted in Flag buffer containing 0.5 mg/ml 3xFLAG peptide ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was purified from the cleared lysate by affinity to Anti-FLAG M2 Affinity gel (Sigma-Aldrich) and eluted with pulldown buffer containing 0.1 mg/ml 3xFlag peptide ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated using 3 kDa (KrACP, FabD) and 30 kDa (KrKSCLF) Amicon Ultra (Millipore, 15 mL capacity) molecular weight cutoff (MWCO ...
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bioRxiv - Immunology 2020Quote: For immunoprecipitation experiments the Chk1 or GATA3 antibody was incubated with Red Protein G Affinity beads (Sigma-Aldrich) for 1 hour at 4°C on a shaker to allow the antibody to bind to the beads ...
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bioRxiv - Biophysics 2021Quote: ... Next, dissolved membrane proteins (1 nmol) and MSP (50 μL, 1 mg/mL) (MSP1E3D1, Sigma-Aldrich, No. M7074) were added to the lipid suspension and mixed for ∼1 h while rotating in the dark at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was recovered and the protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Millipore). 20 μg proteins were loaded on 4-15% Mini-PROTEAN® TGX Precast Protein Gels Stain Free™ (Bio-Rad® ...
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bioRxiv - Microbiology 2021Quote: ... For elution of FLAG-tagged proteins and their complexes 100 µL of 0.2 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Cancer Biology 2021Quote: ... Per sample equal amounts of protein were separated by Bis-Tris-PAGE and transferred to PVDF membranes (Millipore). The membranes were incubated with 5% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-P® PVDF transfer membranes (IPVH00010; EMD Millipore) as previously described (Haynes et al. ...
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bioRxiv - Pathology 2021Quote: ... 2.5-20μg of total protein extracts were separated on acrylamide-SDS gels and transferred to PVDF membranes (Millipore). Specific proteins were detected by using different antibodies listed in supplementary table 1 ...
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bioRxiv - Physiology 2020Quote: ... and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000, Sigma, #S8809), the anti-transferrin receptor mouse monoclonal antibody (TransR ...