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Citations for Millipore Sigma :
7851 - 7900 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... proteins were separated from each sample by centrifugation through Amicon ® Ultra Centrifugal Filters (Merck, Millipore Ltd.) at 21.000 x g for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... All lentiviral vectors (pLKO.1) expressing shRNAs used for knockdown of host proteins were purchased from Sigma.
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation of Flag-tagged proteins were carried out with the anti-FLAG M2 beads (Cat. A2220, Sigma) in RIPA buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... The lyophilized proteins were resuspended in 1 ml of a 2 mg/ml DTT (1,4-Dithiothreitol, Sigma) solution and incubated at 50C for 2h ...
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bioRxiv - Developmental Biology 2019Quote: ... 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3). ∼0.2 μg of GST-OsBZR1 ...
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bioRxiv - Bioengineering 2019Quote: ... total proteins from saliva was ultrafiltrated using an amicon ultra 15 mL centrifugal filter (Millipore, Massachusetts, USA), 20 μg of saliva total protein were subjected to SDS polyacrylamide gel electrophoresis ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated to 30-40 mg/mL using Amicon concentrators (30-kDa cutoff; Millipore).
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bioRxiv - Cell Biology 2019Quote: ... 1 mg of extracted protein in lysis buffer was immunoprecipitated overnight with Flag-M2 affinity gel (Sigma) at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted protein was concentrated using an Amicon-Ultra-15 centrifugal filter (MWCO 30kDa) (Merck Millipore, USA), spin down 15 min in a bench-top centrifuge (4°C ...
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bioRxiv - Plant Biology 2020Quote: ... the protein was supplemented with 100 mM D-glucose before mixing with a 80% monoolein (Sigma-Aldrich) 20% cholesterol mixture ...
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bioRxiv - Systems Biology 2021Quote: ... Yeast proteins digestion was performed on excised bands from gel gradient and digested peptides of UPS2 (Sigma) were used as external standards for absolute protein quantification (more details in Supp ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins underwent binding with secondary antibodies diluted 5000-fold in PBS (anti-rabbit IgG; Sigma-Aldrich). Protein bands were visualized by staining with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Equivalent amounts of protein per sample were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk or 5% BSA in TBST (Tris-buffered saline containing Tween-20 ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were digested overnight at 37°C using trypsin (1:100 w/w, enzyme/protein, Sigma-Aldrich) and LysC (1:100 w/w ...
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bioRxiv - Physiology 2021Quote: ... Protein was detected using a secondary HRP-linked antibody and Luminata Classico Western HRP Substrate (Millipore WBLUC0020). To reprobe membranes ...
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bioRxiv - Microbiology 2019Quote: ... The extracted proteins were subjected to 12% SDS-PAGE and then transferred onto Polyvinylidene Fluoride (Sigma, Germany). The immobilized filter was blocked overnight at 4 °C in 5% skimmed milk in PBST (PBS with 0.05 % (v/v ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Identical amounts of protein samples were subjected to SDS-PAGE and transferred to Immobilon-FL membrane (Millipore). Immunoprecipitated samples were subjected to SDS-PAGE and transferred to Immobilon-FL membrane (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... 20-30 μg protein was separated by 10% SDS-PAGE and transferred to PVDF membranes (Millipore, USA). After being blocked with 5% non-fat milk for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations of wholecell lysates were determined by the QuantiPro™ BCA Assay Kit (Sigma-Aldrich, QPBCA). The proteins were then electrophoresed in SDS-PAGE gels to separate by molecular weight and transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Cell Biology 2019Quote: ... After the run was completed the proteins were stained with Coomassie blue stain (Sigma Cat No-G1041). The gel band from IgG Lane ...
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bioRxiv - Neuroscience 2019Quote: ... Eya1 protein was concentrated to approximately 2 mg/ml using an Amicon Ultra concentrator (30 MWCO, Millipore) and further purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Lifescience ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein complexes were purified using the FLAG-affinity purification approach in the presence of benzonase (Sigma), and analyzed by SDS-PAGE and silver staining before subjected to Mass Spectrometry.
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein (20ug) were loaded onto a 10% polyacrylamide gel and 0.45μM nitrocellulose membrane (Millipore, Billerica ...
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bioRxiv - Bioengineering 2021Quote: ... 15N-labeled protein for NMR analysis utilized M9 media with 13C glucose and 15N ammonium salts (Sigma) as the sole carbon and nitrogen sources ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 ug protein/sample was diluted in 1X LDS Sample Buffer and 50 mM DTT (Sigma-Aldrich), then incubated for 10 minutes at 70°C ...
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bioRxiv - Plant Biology 2021Quote: ... Flag-tagged proteins were detected using the anti-Flag HRP-coupled antibody (mouse monoclonal, A8592; Sigma-Aldrich) diluted 1:5000 (v/v) ...
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bioRxiv - Developmental Biology 2019Quote: ... The target protein levels were normalized to the endogenous control (GAPDH, mouse, Millipore Sigma, MAB374, 1/500). The membranes were scanned on the LI-COR Odyssey CLx imaging system.
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride membranes (PVDF, Merck Millipore) for immunoblot analysis ...
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bioRxiv - Biochemistry 2020Quote: ... Protein complex was incubated overnight on ice and then concentrated onto 30 kDa MWCO Amicon concentrator (Millipore) until protein concentration reached 11 mg/ml ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... and 50 μg of total protein were loaded on 12% acrylamide gel for determination of APP (Sigma), Amyloid-β (Aβ ...
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bioRxiv - Genetics 2021Quote: ... The cell lysate was pre-cleared with 30 μ L Protein A agarose beads (Millipore, 16-156) for 1 hour at 4°C and then incubated overnight on a rotor with antibody coated beads ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 10% SDS-PAGE gels followed by transfer to polyvinylidene difluoride Immobilon membranes (Millipore). Phospho-Erk was detected using a phospho-Erk antibody (clone 4695P ...
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bioRxiv - Neuroscience 2021Quote: ... PLGA) nanoparticles (NP) loaded with the protein (in the control condition bovine serum albumin, BSA, Sigma-Aldrich). The NPs were prepared according to the protocol described by Chaturvedi et al.29 ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 100 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Neuroscience 2021Quote: ... the total protein concentration of each supernatant was measured using a Direct Detect Infrared Spectrometer (Millipore Sigma). Cytokines/chemokines were then quantified by multiplex immunoassay using a high sensitivity mouse multiplex magnetic bead kit (Millipore Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The protein extracts were run on a 5%–12% gel and transferred to nitrocellulose (NC) membranes (Millipore). After blocking in a milk solution (5% powdered milk in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to PVDF membrane (Millipore) by wet transfer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was quantified according to the Coomassie blue method (BRADFORD, 1976) using bovine serum albumin (Sigma-Aldrich) as a standard ...
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bioRxiv - Neuroscience 2020Quote: ... Interactions between CB1 and cytoskeleton proteins were detected using the Duolink in situ PLA detection Kit (Sigma) following the instructions of the supplier ...
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bioRxiv - Biochemistry 2021Quote: Protein carbonyl levels in yeast whole cell lysates were assessed using a colorimetric assay (Sigma-Aldrich, MAK094). WT ...
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bioRxiv - Biochemistry 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
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bioRxiv - Biochemistry 2021Quote: ... The separated proteins were transferred by electrophoresis to polyvinylidene difluoride transfer membranes (Immobilon-P, Millipore, MA, USA). The membranes were treated with diluted affinity-purified anti-p-eIF2α (p-eIF2α ...
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bioRxiv - Synthetic Biology 2021Quote: ... The enzyme and the expressed proteins were inactivated by adding 15mM EDTA (Catalog No. E9884, Sigma-Aldrich) at 75°C for 10 minutes (T100 Thermal Cycler ...
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bioRxiv - Genetics 2020Quote: ... Equal protein loading was confirmed by probing with mouse-anti-β-actin antibody (1:10,000; Sigma, A5441).
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Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5AbioRxiv - Biochemistry 2021Quote: ... and the soluble proteins were incubated with EZview red anti-HA or anti-Flag affinity gel (Sigma) for 3 h at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... was added into 200 µL cell lysate precleared by 20 µL Protein A magnetic beads (EMD Millipore) and incubated overnight at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... LCP) sample was obtained by mixing 40 μl of GtACR1 protein with 60 μl monoolein (MO) (Sigma, St ...