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Citations for Millipore Sigma :
7651 - 7700 of 10000+ citations for Recombinant Human SDC2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... HLA-G2 proteins in PBS were concentrated by Amicon Ultra-15 (NMWL 10,000 Da) (Merck Millipore), and the flowthrough solution from protein concentration was used as a negative control (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μg of protein extract was incubated with 30 μl anti-Flag M2 magnetic beads (Sigma) at 4 C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were prepared with 25 μg of homogenate and 400 ng of yeast enolase protein (Sigma) as an internal protein control ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were prepared with 50 μg of homogenate and 800 ng of yeast enolase protein (Sigma) as an internal protein control ...
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bioRxiv - Biochemistry 2024Quote: ... and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma 16758) at an OD of 0.5 ...
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bioRxiv - Biochemistry 2024Quote: Optional concentration of protein samples using Amicon® Ultra centrifugal filters (Merck Millipore Ltd., Carrigtwohill, IRL) was performed prior to buffer exchange to reduce sample volume ...
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bioRxiv - Biochemistry 2024Quote: ... The extracellular proteins in the filtrate were then concentrated in a 10kDa MWCO Amicon (Millipore Sigma) centrifugal filter ...
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bioRxiv - Biochemistry 2024Quote: ... and the proteins were subsequently transferred to a PVDF membrane (#IPVH00010, Merck Millipore, Burlington, MA, USA). The membranes were washed with TBS-T (20 mM Tris (pH 7.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were transferred to nitrocellulose membranes (Amersham) upon which M2 Anti-FLAG mouse antibodies (Sigma) were used to visualize 3X-FLAG fusion peptides.
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bioRxiv - Biochemistry 2024Quote: Purified proteins were incubated together with anti-FLAG magnetic agarose Beads (Pierce A36797 or Sigma M8823) in 25 mM HEPES pH 7.6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein proximity was analyzed using the Duolink® approach according to the manufacturer’s instructions (Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2024Quote: ... recipient mice were intraperitoneally (i.p) injected with 50µg of OVA protein (Sigma Aldrich, 9006-59-1). On day three following cell transfer ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein expression was induced by adding 0.25 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; [Sigma-Aldrich]) and bacterial cultures were grown at 18 °C for 20 hours ...
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bioRxiv - Microbiology 2024Quote: ... 1% BSA at 4 °C overnight: rabbit anti-microtubule-associated protein 2 (MAP2, Millipore 1: 200); sheep polyclonal anti-dopamine beta hydroxylase (DBH ...
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bioRxiv - Immunology 2021Quote: ... and Tumor necrosis factor-alpha (TNF-α)) were measured with a Milliplex MAP High-Sensitivity Human Cytokine Panel (HSCYTMAG-60SK-13, Millipore Corp., Billerica, MA) on a Luminex MagPix (Millipore Corp. ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293-ACE2 cells [HEK293 cells (ATCC CRL-1573) stably expressing human ACE2]22 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% FBS ...
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bioRxiv - Molecular Biology 2020Quote: Cells were washed three times with saline at 200 × g for 10 min at RT to remove residual Percoll and suspended in RPMI 1640 medium (Cellgro, Manassas, VA) supplemented with 5% human serum (Sigma-Aldrich, St. Louis, MO), unless otherwise stated ...
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bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no. HCVD3-67CKHCYTOMAG-60K, Millipore Corp, St. Charles, MO). According to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and lymph node from BALB/cJ mice and humans were harvested and either fixed for 48 h at RT in 10% neutral buffered formalin (Sigma-Aldrich, St. Louis, MO) or zinc-fixation buffer (pH 6.5 - 7) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse and human brain slices of 20 μm thickness were subjected to antigen retrieval in 10 mM citrate (Sigma-Aldrich, St. Louis, MO, USA) + 0.5% Tween-20 (Merck ...
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bioRxiv - Biophysics 2020Quote: ... Fibronectin coating was performed by covering the glass slides in a solution of DPBS with 10 μg/mL human fibronectin (EMD Millipore, Burlington, MA, USA) for at least 2 hours ...
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bioRxiv - Cell Biology 2021Quote: Labeling of septins on Western blots (WB) and/or on immunocytochemistry coverslip (ICC) was achieved with the following antibodies: rabbit polyclonals against human Sept2 (Sigma-Aldrich, Cat#HPA018481, WB), against human Sept9 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Human chorionic gonadotropin (hCG) as an effective Lh (Luteinizing hormone) mimicking hormone used in this study was purchased from Sigma-Aldrich (Oakville, ON, Canada). There is evidence that hCG transactivate Lhcgr (Luteinizing hormone/choriogonadotropin receptor ...
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bioRxiv - Cancer Biology 2022Quote: ... Both these constructs were individually transfected in Ishikawa cells (human endometrial epithelial cells) using Xtreme Gene HP transfection reagent (Sigma Aldrich; Missouri, United States) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Cytokines in BAL fluid samples were measured (pg/ml) in singlicate by Luminex using a Millipore human cytokine multiplex kits (EMD Millipore Corporation, Billerica, MA) according to manufactures instructions ...
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bioRxiv - Immunology 2021Quote: Cytokine and chemokine assays were performed by the Forsyth Multiplex Core (Cambridge, MA, USA) using the Human Cytokine/Chemokine Magnetic Bead Panel (Milliplex, Millipore Sigma, Burlington, MA, USA) and Bio-Plex®200 plate reader following manufacturers’ specifications ...
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney Vero’76 and human lung adenocarcinoma Calu-3 cells were maintained in DMEM (Sigma-Aldrich, D5796; St. Louis, MO, USA) supplemented with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: Levels of cytokines/chemokines in macaque plasma were measured using the Milliplex MAP Non-human Primate Cytokine Panel in combination with a Luminex 200 (Millipore Corp., Billerica, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2020Quote: HeLa cells were purchased from the Human Science Research Resources Bank and maintained in DMEM (Nacalai tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO). BVRA-KO HeLa cells (HeLa/BVRA KO ...
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bioRxiv - Pathology 2019Quote: ... and TGF-β1 in the samples was measured using magnetic bead-based multiple immunoassays (MILLIPLEX® MAP kit, Human High Sensitivity T Cell Magnetic Bead Panel: EMD Millipore Corporation, Germany). Each assay included two calibration curves for each of the proteins to be measured (calibration ranges ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in Minimum essential medium Eagle (Sigma-Aldrich, cat# M4655-500ML) containing 10% FCS and 1% PS ...
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bioRxiv - Immunology 2021Quote: TEER was measured in the human airway epithelium inserts to determine the integrity of tight junctions with a Millicell ERS-2 volt-ohmmeter (Millipore Sigma, Burlington, MA, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in Minimum essential medium Eagle (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FCS and 1% PS.
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bioRxiv - Microbiology 2022Quote: ... Cells were resuspended in 100 μl of IntestiCult™ OGM Human with 10 µM Y-27632 and seeded in collagen IV (Sigma-Aldrich, cat#. C5533) pre-coated 96 well plates ...
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bioRxiv - Neuroscience 2022Quote: MDCKII cells in which the endogenous canine MDR1 (cMDR1) gene was replaced with a heterologous human MDR1 (hMDR1) (catalog number MTOX1303, Millipore Sigma, Burlington, Massachusetts, USA) were maintained in high glucose Dulbecco’s Modified Eagle Medium (catalog number 11965092 ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Cell Biology 2022Quote: ... the dishes were coated with a freshly made 2 ug/mL solution of fibronectin diluted in phosphate buffered saline (PBS) from a stock of 0.1% human plasma fibronectin (Sigma Aldrich, Cat. no. F-0895). The fibronectin coating was applied overnight at 4oC ...
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bioRxiv - Cell Biology 2022Quote: IMR-90 normal human fibroblasts (ATCC, CCL-186) and HEK293T cells (ATCC, CRL-3216) were cultured in DMEM (Sigma–Aldrich, D5796; Wako, 044-29765) supplemented with 10% FBS (BioWest ...
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bioRxiv - Microbiology 2022Quote: ... HOS cells stably expressing human ACE2 and TMPRSS2 (Ferreira et al., 2021; Ozono et al., 2021) were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Bioengineering 2024Quote: For podocyte stimulation the intact kidney was perfused through the arterial catheter with 250 µl of 10 µM human angiotensin 2 (Sigma-Aldrich, Darmstadt, Germany, A9525), dissolved in custom kidney buffer (10 mM NaHCO3 ...
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bioRxiv - Cell Biology 2024Quote: ... and rabbit polyclonal anti-β-actin antibody (catalog number A2066, epitope: amino acids 365-375 of human β-actin) were obtained from Sigma (Madrid, Spain). Mouse monoclonal Anti-GOK/STIM1 antibody (Clone 44/GOK ...
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bioRxiv - Microbiology 2024Quote: ... using 100 mg of human stool sample per 1 ml of PBS supplemented with 0.1% L-cysteine and 15% glycerol (Sigma-Aldrich, St-Louis, MO, USA). The suspension was incubated for 10 min to allow large particles to settle ...
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bioRxiv - Immunology 2024Quote: ... followed by washing with pre-warmed PBS and incubation for another 5 days in RPMI medium supplemented with 10% human AB serum (HS, Sigma-Aldrich, St. Louis, MO), 100□U/ml penicillin ...
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bioRxiv - Developmental Biology 2023Quote: ... Cumulus oocyte complexes were harvested 14 h after hCG injection and placed in human tubal fluid (HTF) medium supplemented with 4 mg/ml bovine serum albumin (BSA, A3311; Sigma-Aldrich, St. Louis, MO) covered with paraffin oil (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2023Quote: ... Then we added a series of two-fold dilutions of purified human SP-A (0-10 µg/ml, 100µl/well) in TBST containing either 5 mM CaCl2 (Sigma-Aldrich, Saint Louis, MO, USA) or 10 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: The following primary and secondary antibodies were used at indicated concentrations: anti-human α-actinin1 (mouse monoclonal, BM75.2, Sigma Aldrich, A5044; WB 1:1000), anti-human talin (mouse monoclonal ...
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bioRxiv - Cell Biology 2023Quote: 3μm sections of formalin fixed and paraffin-embedded (FFPE) human carotid artery samples from the Munich Vascular Biobank25 were mounted on 0.1% poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) pre-coated SuperFrost Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... the cells were spun down and the supernatants were collected for analysis by Human T Cell 17-plex panel (Sigma-Aldrich, Saint Louis, Missouri). For NK cell stimulation ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies ...