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Citations for Millipore Sigma :
701 - 750 of 1332 citations for SARS Coronavirus Spike Glycoprotein S1 His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the His6-tag was proteolytically removed using 5 U of thrombin (Sigma-Aldrich) per mg protein overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Western blotting was performed using anti S-tag (Cat no. 71549-3, Novagen) and anti His-tag antibody to detect protein-protein interaction.
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal HA-Tag and Mouse Monoclonal Flag antibodies purchased from Sigma (USA); Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
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bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
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bioRxiv - Cell Biology 2021Quote: ... containing 50 μM Phos-tag AAL-107 (Wako) and 10 mM MnCl2 (Sigma) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated with an anti-HA epitope tag rabbit pAb (H6908, Sigma) and ...
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bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
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bioRxiv - Microbiology 2019Quote: ... or rat horseradish peroxydase-conjugated antibody against HA-Tag (Clone 3F10, Sigma-Aldrich). Quantifications were performed by means of the luminescence image analyzer Image Quant Las 4000 mini and Image Quant software (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... Sections were stained with antibodies against the HA-tag (1:500; Sigma; RRID:AB_260070), pSTAT3 (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... the 2xStrep tag was not removed by incubation with HRV 3C protease (Millipore), and proteins were not purified by size exclusion chromatography.
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bioRxiv - Microbiology 2020Quote: ... then the 2xStrep tag was removed by cleavage with HRV 3C protease (Millipore) overnight ...
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bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies against Flag-tag (F3165, 1:3,000) was obtained from Sigma-Aldrich.
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bioRxiv - Cell Biology 2022Quote: Enzymatic cleavage of the GST tag was performed using thrombin (Cat# T1063, Sigma). Supernatants containing cleaved target proteins were incubated with Benzamidine Sepharose 4 flat flow (Cat# 17-5123-10 ...
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the FLAG tag (A8592, Sigma-Aldrich, clone M2, monoclonal), PAP (P1291 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-FLAG-tag (1:5,000, mouse mAb, F1804, Sigma-Aldrich, St. Louis, MO), anti-alpha-tubulin (1:2,000 ...
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bioRxiv - Cell Biology 2023Quote: ... and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich). The purified proteins were exchanged to the biotinylation buffer containing 25 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... Triacylglycerol (TAG, Cat# T7140) and monoacylglycerol (MAG, Cat# M2015) were purchased from Sigma. β-Glucosylceramide (β- GlcCer ...
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bioRxiv - Microbiology 2023Quote: Antibodies were as follows: commercial mouse monoclonal antibodies to Flag-tag (M2; Sigma), Strep-tag (4F1 ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of the cleared protein sample were mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich) diluted in 250 μl 1.25x protein loading buffer ...
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bioRxiv - Genetics 2023Quote: ... and NTERA-2 cells (for ChIP-Rx spike-in) were grown on TC-treated culture dishes in high glucose Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2019Quote: ... The DNA encoding AtGLR3.2 residues Ser420-V572 (S1) and P682-N811 (S2) was amplified using gene-specific primers and subcloned into the pET22b vector (Novagen) between NcoI and XhoI sites with a GT linker between S1 and S2 (Armstrong and Gouaux ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was changed to S1 medium consisting of basal medium supplemented with 6 μM CHIR99021 (Sigma, Cat No. SML1046). Basal medium was prepared by adding 1x GlutaMax supplement (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... 5A and S1) were carried out on 0.8% w/v LB Agarose supplemented with 1 µg/mL propidium iodide (PI; Sigma-Aldrich). Colony monolayer assays (Figure 3 ...
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bioRxiv - Systems Biology 2020Quote: ... and His6-tagged GFP gene (primers A18 and 19; Table S1) were separately cloned using InFusion (Clonetech) methods into NdeI/BamHI-linearised pET19b (Novagen).
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with anti-human antibodies (see Table S1 for additional details) diluted in FACS buffer (DPBS supplemented with 1% FBS and 0.05% sodium azide (Sigma-Aldrich). Samples were analysed using a BD FACSCanto II Flow Cytometer (BD Biosciences) ...
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bioRxiv - Biochemistry 2021Quote: ... bacteriovorus HD100 genomic DNA using primers detailed in Table S1 Amplified construct DNA was inserted into a modified pET41c plasmid (Novagen, GST-tag removed and thrombin cleavable 8xHis-tag introduced at C-terminus ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng CRISPR-Cas9 (PX458) plasmid having the right sgRNA insert (Table S1) was transfected along with transfection reagent Lipofectamine 2000 (Sigma) at a 1:3 DNA to reagent ratio ...
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bioRxiv - Molecular Biology 2020Quote: The phosphorylation of the S1/S2 linker constructs was examined in vitro using purified cyclin B-Cdk1 (Millipore 14-450) and PKA (murine cAMP-dependent protein kinase) ...
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bioRxiv - Bioengineering 2023Quote: ... were mixed with ssDNA output oligonucleotides of interest at 2x excess (Table S1) in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG ...
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bioRxiv - Molecular Biology 2024Quote: cDNAs of BTB domains were PCR-amplified using corresponding primers (Supplementary Table S1) and cloned into modified pET32a(+) vector (Novagen) encoding TEV protease cleavage site after 6xHis-tag and Thioredoxin ...
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bioRxiv - Immunology 2020Quote: ... the SARS-CoV-2-RBD-binding phages were eluted with 100 mM triethylamine (Sigma-Aldrich, Xuhui, Shanghai). All eluted phages were collected and used to infect TG1 cells ...
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bioRxiv - Immunology 2021Quote: ... Eluted SARS-CoV-2 S containing fractions were concentrated using Amicon Ultra (cut-off: 30 KDa) (Millipore) and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with anti-6x His antibody horseradish peroxidase (HRP) conjugate (Sigma A7058-1VL ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant containing 20mM imidazole was incubated with Ni-NTA His•Bind® Resin (Novagen) beads with end-to-end shaking for 4 hr at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cleared lysates were loaded on chromatographic columns filed with His-select resin (Millipore Sigma) or glutathione resin (GE healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Ni-NTA pull down was conducted by using HIS-Select Nickel Affinity Gels (Sigma). After lysing cell pellet with buffer A (6 M guanidine-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... HIS-tagged B subunit of Shiga toxin 1(SML0655) and Apilimod (A149227) from Sigma; rabbit anti-EEA1 (#3288) ...
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bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich) and 1% antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... and the subsequent western blot analyses were performed with anti-His (Sigma, Missouri, USA) or anti-FLAG antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...