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Citations for Millipore Sigma :
501 - 550 of 1332 citations for SARS Coronavirus Spike Glycoprotein S1 His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... GST tag was then cleaved off using preScission protease (Sigma) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... HSV tag (Sigma-Aldrich H6030, 1:1,000 or 1:2,000), Rpb1-Core (Y80 Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG tag (M2) and α-tubulin (T5168) from Sigma-Aldrich; βIII-tubulin from Biolegend (801201).
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag was cleaved with restriction-grade thrombin (Millipore) overnight on ice in Tris buffer supplemented with 2.5 mM CaCl2 ...
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bioRxiv - Neuroscience 2022Quote: ... The HA tag oligonucleotide strand was synthetized by Sigma-Aldrich as two complementary single-stranded oligonucleotides (Supplementary Table 5) ...
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bioRxiv - Microbiology 2019Quote: HA Tag-FITC (7 ug/mL) (Sigma, clone HA-7)
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-FLAG tag (clone M2-F1804, Sigma, 1:1000), mouse anti-GFP (clones 7.1 and 13.1 ...
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bioRxiv - Biophysics 2021Quote: ... The MBP tag was cleaved with Thrombin (Sigma-Aldrich, USA) at 0.4 unit per mg of protein ...
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bioRxiv - Cell Biology 2022Quote: ... then HRP tags were activated with enhanced chemiluminescence solution (Millipore). Signal was detected and imaged via ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... anti-Flag or anti-Pk (V5 Tag) antibodies (Sigma-aldrich) were used for detecting epitope-tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... which carries the N and C-terminal 6xHistidine tags (Novagen), using the Hi-Fi DNA assembly kit (New England Biosciences).
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bioRxiv - Cell Biology 2022Quote: TAG quantification was performed using Triglyceride Quantification kit (Sigma-Aldrich). For TAG quantification of whole body ...
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bioRxiv - Microbiology 2023Quote: ... An anti-histidine tag antibody (Sigma, St. Louis, MO, USA) and serum from the immunized mice were used as positive controls ...
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bioRxiv - Microbiology 2023Quote: ... or with rabbit primary antibodies against HA-tag (Sigma-Aldrich) followed by anti-rabbit HRP-conjugated secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... rabbit polyclonal against FLAG tag at 1:500 (F7425, Millipore), rabbit polyclonal against Myc tag at 1:2000 (ab9106 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-HA-Tag (1:500, rabbit Sigma-Aldrich H3663), anti-FLAG (1:50 ...
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bioRxiv - Microbiology 2021Quote: BHK-SARS-2R_GFP_NeoR_NL cells were trypsinized and washed twice with DPBS (Sigma-Aldrich) and once with sort buffer (PBS supplemented with 0.3 % BSA ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was carried out using LiTIMFP and LiTIMRP (Table S1. Sigma-Aldrich Co, India) as the forward and the reverse primers ...
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bioRxiv - Microbiology 2021Quote: ... S1-containig cell culture media was incubated with 10 μL of anti-Flag beads (Sigma, F2426) to precipitate S1 subunit ...
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bioRxiv - Neuroscience 2023Quote: ... Total supernatant (S1 + S2) was passed through a 10 μm hydrophobic PTFE filter (LCWP02500, Merck Millipore) and centrifuged for 1 min at 4,000 g ...
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Evidence for Involvement of WDPCP Gene in Alcohol Consumption, Lipid Metabolism, and Liver CirrhosisbioRxiv - Genetics 2023Quote: ... were incubated together with either 35 µL of PBST (for free glycerol measurements) or 35 µL of TAG reagent (Sigma, no. T2449, for TAG measurements) at 37 °C for 60 min ...
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bioRxiv - Microbiology 2021Quote: Vero cells were transfected with spike protein expression constructs using the GeneJuice transfection reagent (Novagen). The indicated S-protein expression constructs (1 µg ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Immunology 2020Quote: Cell lines (HEK293T, HEK293, 3C11, 143B, MDCK-SIAT1, MDCK-PR8) were maintained in DMEM (Sigma Aldrich) supplemented with 10% FCS (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells overexpressing Flag-AurkB was treated with 10 µM of trichostatin A (TSA) (Sigma-Aldrich) or DMSO for 12 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Bioengineering 2021Quote: ... for HEK293-T and SW480 cells were coated with fibronectin bovine plasma (F1141-2MG; Sigma Aldrich) before seeding cells onto the 8-well plates ...
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bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were seeded in 8-well Nunc Lab-Tek II chamber slides (0,7 cm2, Sigma) at a density of 70.000 cells/cm2 ...
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bioRxiv - Neuroscience 2019Quote: ... and PAK/cofilin mechanism experiments used human embryonic kidney cells (HEK293) cultured in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Immunology 2020Quote: ... Protein production was done in HEK293-6E cells by transient transfection with polyethylenimine (PEI, Sigma-Aldrich) and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: 2×105 HEK293 were seeded onto 24well plates previously pre-coated with poly-L-lysine (Sigma), and infected with 2×105 ...
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bioRxiv - Immunology 2020Quote: ... this mixture was applied to mammalian FreeStyle™ HEK293-F cell culture (EMD-Millipore, Darmstadt, Germany) at a density of 1 million cells per milliliter ...
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bioRxiv - Neuroscience 2023Quote: ... and mock plasmids were transfected into HEK293 suspension cells derived from tsA201 (Millipore Sigma, 96121229-1VL) using Polyethylenimine (Polysciences ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...