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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Cells were incubated with recombinant Cholera toxin B (CTxB)-FITC (Sigma Aldrich C1655) for 1 h to stain for lipid rafts and then with an anti-TLR4 antibody (Abcam ab22048 ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant plasmids were transformed into BL21 (DE3) or BL21 (DE3) Tuner cells (Novagen) and were incubated in 1L LB in 2L conical flasks at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 constructs were visualised with primary antibody mouse α-FLAG (F1804; 1/200; Sigma) for 2 hours at 37°C in a humidified chamber ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse anti-human nuclei clone 235-1 (1:250; Millipore), rabbit anti-histone H3.3K27M mutant (1:500 ...
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bioRxiv - Immunology 2019Quote: ... and rabbit anti-human ZO-1 (1:500, HPA001636 Sigma) primary antibodies were used to probe the membrane ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-human nuclei clone 235-1(1:100; Millipore), or rabbit anti-Ki67 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-human nuclei clone 235-1 (1:100; Millipore), rabbit anti-Ki67 (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... SUM149PT were grown in SUM medium (1:1 mix of DMEM/F12 and Human Mammary Epithelial Cell Growth Medium (Sigma), 5% FBS ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells isolated from healthy donor PBMCs were stimulated by a 1:1 ratio of anti-CD3/28 activation beads (Miltenyi) in complete human T cell medium (RPMI1640 from Sigma containing 2 mM glutamine with the addition of 1 mM pyruvate ...
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bioRxiv - Microbiology 2021Quote: ... Precleared lysates containing the differently tagged or untagged proteins were mixed in a 1/1 ratio and incubated with anti-FLAG-agarose beads (Sigma) overnight at 4°C to precipitate the FLAG-tagged proteins ...
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bioRxiv - Immunology 2019Quote: ... cells were overlaid with a 1:1 mixture of 2% agarose:2X 199 Media (Sigma-Aldrich) containing 10 μg/mL chymotrypsin ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were washed 2 x with PBS and 1 x with DAPI (1:2000, Sigma) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Microbiology 2023Quote: ... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Cell Biology 2022Quote: The human alveolar carcinoma epithelial cell line A549 (ATCC, CCL-185) and human colorectal adenocarcinoma cell line HT29 (Sigma Aldrich) were utilized for toxicological evaluation ...
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bioRxiv - Pathology 2019Quote: ... Human dermal fibroblast cells (HFC, primary cells, Sigma Aldrich, Buchs, Switzerland) were cultivated in DMEM supplemented with 15% FCS and 1% PSN/L-glutamine ...
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bioRxiv - Physiology 2023Quote: The LX2 human stellate cell line (LX2 cells; Millipore Sigma, SCC064) was maintained in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...
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bioRxiv - Developmental Biology 2020Quote: ... HA-tagged or Myc-tagged proteins were isolated with anti-HA agarose or anti-c-Myc Agarose Affinity Gel (Sigma), respectively ...
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Bioengineering 2019Quote: Ni-NTA columns were prepared by packing 1 mL Ni-NTA His•Bind resins (Sigma) into a 6-ml ISOLUTE® Single Fritted Reservoir column with 10 μm polyethylene frit (Biotage ...
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bioRxiv - Cell Biology 2021Quote: ... and a western blot was performed using anti-HIS (Sigma H-1029, mouse, 1:3000) and anti-HA (Biolegend MMS-101R or ...
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... neoformans cells were added to washed macrophage monolayers at a 1:1 ratio with 10 units (1 unit = 0.1 ng/mL) of recombinant murine IFNγ (Sigma) and 0.3 μg/ml of lipopolysaccharide (Sigma ...
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bioRxiv - Immunology 2020Quote: ... recombinant GM-CSF (10ng/ml; Peprotec) and β-estradiol (1 μM; Sigma-Aldrich). To differentiate into macrophages ...
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bioRxiv - Immunology 2022Quote: ... Cells were treated with EGFP tagged esiRNA that served as an NC (NC-esiRNA-EGFP, Sigma-Aldrich). About ...
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bioRxiv - Cell Biology 2022Quote: ... Human cells were pretreated with glycine (Millipore, 104201) at a concentration of 50 mM for at least 10 minutes before stimuli was added.
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bioRxiv - Systems Biology 2022Quote: ... Human cervix epithelioid carcinoma cells (HeLa; Sigma Aldrich) were cultured in Dulbecco’s Modified Eagle’s (DMEM ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were treated with human PRL (Sigma, #L4021) at 250ng/mL for 5 ...
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bioRxiv - Neuroscience 2021Quote: SH-SY5Y human neuroblastoma cells (Sigma-Aldrich #94030304) at P11 were transduced in a 6 well plate using 300 µl of Auto-hLAG3 LV pre-incubated with 30 µl of Lenti-X™ Accelerator (Clontech #631256 ...
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bioRxiv - Cell Biology 2019Quote: HEK293T human embryonic kidney cells (Sigma Aldrich, #12022001) stably expressing N-terminally HA-tagged BAG3 were harvested and lysed with 3 ml lysis buffer (50 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a human intestinal epithelial cell line (Sigma-Aldrich) was grown at 37 °C in D-MEM containing 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with human IFNβ (EMD Millipore) at a concentration of 40 units/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Adenocarcinoma human alveolar basal epithelial A549 cells (Sigma) were cultured in Ham’s F-12 K medium (Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were blocked with human IgG (Sigma I4506) in Dulbecco’s PBS (ThermoFisher Scientific 14190136 ...
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bioRxiv - Cell Biology 2023Quote: ... Purified cells were plated onto human fibronectin (Sigma) coated plates in iEC medium ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Human skeletal muscle cells (HSMC, Sigma, #150-05F) were maintained in growth medium (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... GST tagged was bought from Sigma-Aldrich (SRP5172). G-actin and biotinylated actin were bought from Cytoskeleton ...
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bioRxiv - Neuroscience 2021Quote: ... the proteins were tagged with FITC (Sigma, F7250) before encapsulation.
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bioRxiv - Cancer Biology 2022Quote: ... HRP-tagged anti-goat secondary antibody (Sigma-Aldrich), GFP ...
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bioRxiv - Cancer Biology 2020Quote: Both pBabe-SAOS 2 and hTERT-SAOS 2 (107 cells) were cross-linked with 1% paraformaldehyde (Sigma Aldrich, # P6148) in culture medium for 10 min at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... The ligand phenylethyl β-D-thiogalactopyranoside (PETG) was purchased from Sigma-Aldrich (catalog #P1692) and prepared as described38 ...
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bioRxiv - Immunology 2022Quote: ... 2×105 cells of each of transfected and untransfected cells stained with SARS-CoV-2 Spike antibody (SinoBiological) and Anti-human IgG FITC (Sigma-Aldrich®). After staining with primary and secondary antibodies ...