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Citations for Millipore Sigma :
701 - 750 of 4279 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Genetics 2024Quote: ... supplemented with 10% Heat Inactivated Fetal Bovine Serum (HI-FBS) (Sigma, REF-F4135), 1% L Glutamine 200 mM (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Molecular Biology 2024Quote: 37.5 μL of mix buffer equilibrated His-select cobalt affinity resin (Sigma-Aldrich) was used for each pull-down ...
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bioRxiv - Biochemistry 2024Quote: ... cleared cell lysates were applied to Ni-NTA His-bind resin (Merk Millipore) equilibrated in gravity columns ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Cancer Biology 2021Quote: ... All FLAG-tagged proteins were purified with anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) in a native condition using the FLAG peptide elution ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).
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bioRxiv - Physiology 2021Quote: ... followed by incubation with fluorescent-tagged carboxylate-coated 2 um size latex beads (L3030, Sigma) for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-tagged Pros-S-HD in soluble fraction was purified using Glutathione-Agarose beads (Sigma). The bound proteins were eluted in elution buffer (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Genetics 2022Quote: V5H6-tagged FRQ was immunoprecipitated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog #7345) from 2 mg of centrifugation-cleared lysate ...
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bioRxiv - Microbiology 2022Quote: ... and FLAG tagged S-protein was stained using anti-FLAG (clone M2, Sigma-Aldrich, F1804). SARS-CoV-2 Spike Antibody (ProSci ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD3 and PFD5 were detected using anti-FLAG rabbit antibody (1:5,000, Sigma), FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000 ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Molecular Biology 2023Quote: ... myc-tagged TEV protease was detected with a mouse monoclonal anti-myc antibody (Sigma M4439). The proteins were detected using SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Immunology 2023Quote: ... Co-IP of FLAG-tagged proteins was performed by incubating M2 anti-FLAG resin (Sigma) with clarified cell lysates for 2 h at 4°C with end-over-end rotation ...
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bioRxiv - Cell Biology 2024Quote: ... Flag-tagged Scube2 was detected by using polyclonal α-FLAG antibodies (Sigma, St. Louis, USA). GAPDH ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing pure polyhistidine-tagged proteins were pooled and concentrated by centrifugation filtration (EMD Millipore; using appropriate MWCO ...
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bioRxiv - Cancer Biology 2024Quote: ... lysates containing HA-Ub tagged DUBs were immunoprecipitated with anti-HA agarose beads (Sigma-Aldrich) for 16h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... or 100% C3-depleted human serum (C3-depleted serum, human; Millipore Sigma) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Recombinant GST-p50 subunit D434-969 was purchased from Sigma-Aldrich. Binding reactions were performed for 20 minutes at room temperature with 20 pmol annealed primers ...
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bioRxiv - Immunology 2021Quote: Recombinant cytokines were purchased from indicated suppliers: IFN-β1a (Sigma, #IF014); IFN-γ (Sigma ...
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bioRxiv - Genomics 2020Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: Recombinant HSA expressed in Pichia pastoris was purchased from Sigma-Aldrich (#A7736 ...
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bioRxiv - Neuroscience 2021Quote: ... and with or without 0.3 µM recombinant BirA (Sigma-Aldrich; SRP0417) (Howarth and Ting ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Systems Biology 2020Quote: ... Recombinants were selected on LB-agar containing 15μg/ml kanamycin (Sigma). Successful integration was confirmed via amplification and sequencing of the flu locus ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant TNFα was purchased from Sigma-Aldrich (St. Louis, MO, USA). The protease inhibitor cocktail was purchased from Thermo Fisher Scientific (MA ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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bioRxiv - Cell Biology 2022Quote: For binding assays with recombinant KAP (Sigma Aldrich, Cat. No. SRP5175) or CAK (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
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bioRxiv - Immunology 2022Quote: ... cells were then stimulated with 100U/ml recombinant IFNα2 (Sigma-Aldrich) to induce transcription from the IFIT1 loci ...
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bioRxiv - Biochemistry 2022Quote: ... 1000 U/mL recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore) and 50 U/mL Penicillin–Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Biochemistry 2020Quote: ... Recombinant rabbit anti-pan-ADP-ribose binding reagent MABE1016 (Merck Millipore) was used 1:1000 ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) for CSR to IgG1 ...
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bioRxiv - Genomics 2022Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Systems Biology 2024Quote: ... and 500 U/mL recombinant leukemia inhibitory factor (LIF; Millipore, ESG1107) at 37 °C in 5% CO2 ...