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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for 6 Isopropyl 2 methylbenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Oocytes were mounted in VectaShield containing 0.75 μg/ml 4-6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, D9542). Imaging was performed using a Zeiss Axio Observer Z1 microscope with AxioCam MRm Rev3 camera and ApoTome optical sectioning (Carl Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were counterstained with 5 μM 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich, Cat# D9542) in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... the nuclei were labeled with a 1:10,000 dilution of DAPI (4’,6-Diamidino-2-phenylindole; Sigma, D9542) in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... coverslips were incubated 6 min in PBST containing a final concentration of 2 µg/mL DAPI (Sigma-Aldrich) and washed three times for 10 min with PBST ...
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bioRxiv - Cell Biology 2019Quote: ... DNA staining was achieved by addition of 1 μg/ml 4',6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Coverslips were mounted using mounting medium (20 mM Tris pH8 ...
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bioRxiv - Microbiology 2021Quote: ... Sections were counterstained with 4′,6-Diamidino-2-phenylindole di-hydrochloride (DAPI, Sigma-Aldrich, St. Louis, MO, USA) diluted 1:3.000 in PBS ...
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bioRxiv - Plant Biology 2021Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich catalogue no D9542, 1:600 dilution). Figure 4 shows images of seedlings from 1 of 6 experiments ...
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bioRxiv - Pathology 2021Quote: ... and then they were incubated with 100 ng/ml 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) in PBS before mounting with ImmunoSelect antifading mounting medium (Dianova) ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were stained by addition of 1 µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich) containing FluoromountG (Southern Biotech) ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were treated with PBS containing 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich, cat# D8417) for 20 min and mounted with cover glass using Fluorogel (Electron Microscopy Sciences ...
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bioRxiv - Cancer Biology 2022Quote: ... the cover slides were treated with 1 mg/ml N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate (Sigma) and exposed to ultra-violet (UV ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then treated with 4′,6-Diamidine-2′-phenylindole dihydrochloride at 15mM (DAPI, Sigma-Aldrich, Madrid, Spain) during 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... at RT and nuclear counterstaining was performed using 4’,6-diamidino-2-phenylindole (DAPI, 1.5 μg/mL; Sigma). After brief drying ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:10,000 dilution with milliQ water, Sigma-Aldrich) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded in 6 well plates coated with Poly(2-hydroxyethyl methacrylate) (Poly-HEMA, Sigma Aldrich, P3932) and afterwards lysed ...
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bioRxiv - Neuroscience 2019Quote: Zebrafish 4-6 dpf larvae were immobilized right side up in 2% low melting point agarose (Sigma-Aldrich) on microscope slides ...
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bioRxiv - Genomics 2019Quote: ... Cells were also labelled with a combination of 4′,6-diamidino-2-phenylindole or DAPI (Sigma, Cat #D9542) and Wheat Germ Agglutinin (WGA ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by appropriate fluorescently conjugated secondary antibodies and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma, D9542). Primary antibodies used were against ...
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bioRxiv - Physiology 2020Quote: ... Nuclei were counter-stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 1/5000) (Sigma-Aldrich, Dorset, UK). Imaging was performed on a Leica TCS SP5 confocal microscope (Leica Microsystems ...
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bioRxiv - Microbiology 2021Quote: ... T13320), SYTOX green (0.25μM, Thermo, S7020) and 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI, 2μg/ml, Sigma, D9542). Staining was performed at ambient temperature for 20 minutes in the dark followed by a wash with 50μl PBS ...
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bioRxiv - Immunology 2020Quote: Cell suspensions were stained for viability with either 1:3000 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) or 1:1000 Zombie Aqua (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 5 min with 10 μg/ml 4’,6-Diamidino-2-phenylindole (DAPI; Sigma-Aldrich) to stain cell nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were stripped by 2 x 7 min incubations in stripping buffer (6 M guanidine hydrochloride (Sigma: G3272) with 1:150 β-mercaptoethanol ...
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bioRxiv - Neuroscience 2022Quote: ... with 1:30,000 of the fluorescent marker of nucleic acids 4’,6-diamino-2-fenilindol (DAPI; Sigma-Aldrich). We took photographs of the cells using an epifluorescence microscope (DMIL LED ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were lastly counterstained with DAPI (4′,6-diamidino-2-phenylindole) and mounted on slides using DABCO (Sigma). Cells were then viewed using a Nikon Ti2-E/A1R-Multiphoton microscope equipped with DS-Qi2 camera (Nikon).
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bioRxiv - Microbiology 2022Quote: ... Nuclei were labeled using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then treated with 4′,6-Diamidine-2′-phenylindole dihydrochloride at 15mM (DAPI, Sigma-Aldrich, Madrid, Spain) during 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... washed 2 x in Gurr buffer and stained with 30% Giemsa stain solution (Sigma-Aldrich, #51811-82-6) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were labeled using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml). The immunostained lymph node tissues were scanned using an Olympus VS-120 slide scanner with a Hamamatsu ORCA-R2 C10600 digital camera ...
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bioRxiv - Microbiology 2023Quote: ... NR-6226C (Terpyridine (2,2′:6′,2′′-Terpyridine, 98%)) was purchased from Sigma Aldrich (now Merck, KGaA, Darmstadt Germany). Metal solutions containing 2 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated in blocking buffer containing 0.8 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Sigma, cat # D9542) for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... nuclei were stained with 4ʹ,6-diamidino-2-phenylindole (DAPI) and mounted in Mowiol (Sigma-Aldrich, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The plate was then rinsed with PBS and counter-stained with 4′,6-diamidino-2-phenylindole DAPI (Sigma) for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated with DAPI (4’,6-Diamidino-2-phenylindole dihydrochlorid) solution(D9542, Sigma-Aldrich, diluted 1:10000) for 7 minutes at RT and subsequently mounted in ProLong Gold Antifade mounting medium (cat ...
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bioRxiv - Genetics 2023Quote: ... were used as secondary antibodies and sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Mounting was performed using Prolong Gold Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Enriched proteins were denatured in U/T buffer (6 M urea, 2 M thiourea, 1 mM DTT (Sigma), 10 mM HEPES ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were routinely passaged in a 1:6 ratio every 2 days using 1× Trypsin–EDTA solution (Sigma) at 37°C and the reaction was stopped using 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated in the DNA fluorescent stain DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich, D9542) at a concentration of 1 µg/ml in 1x PBS for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 0.04 mg/ml catalase (EMD biosciences) and 1 mM 6-hydroxy-2,5,7,8-tetramethyl-chromane-2-carboxylic acid (Trolox) (Sigma Aldrich). After DNA was tethered on the surface ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed and the nuclei counter stained with 40-6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... were used as secondary antibodies and sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Mounting was performed using Prolong Gold Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) 1:1,000 (Sigma-Aldrich Sweden AB, Stockholm, Sweden). Invasion of the cells was measured by counting the nuclei of the cells that migrated through the filter pores towards the chemoattractant (6% FBS) ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were cultured at 37°C overnight and induced by addition of 0.2 mM isopropyl-1-thio-β-d-galactoside (Sigma-Aldrich) for 4 hours at 28°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were plated onto selective Lysogeny broth (LB)-agar plates supplemented with 1 mM Isopropyl b-D-1-thiogalactopyranoside (IPTG) (no. I6758; Sigma-Aldrich and 50 µg mL−1 of 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-Gal ...
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bioRxiv - Biochemistry 2021Quote: ... Bacteria were grown to an OD600 of 0.8 in lysogenic broth (LB (Luria/Miller)) medium (Carl Roth) before the expression was induced with 0.5 mM isopropyl-β-D-thiogalactopyranoside (IPTG, Sigma-Aldrich). Following overnight expression at 18°C ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 transformed with recombinant pGEX-2T plasmids were diluted 1:10 with fresh medium and grown for 1 h at 37°C before inducing with 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG, Sigma). After another 4 h incubation at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Microbiology 2020Quote: ... The temperature was subsequently reduced to 18°C and protein production was induced with 0.5 mM Isopropyl-β-D-thiogalactoside (IPTG, Sigma Aldrich) overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells were grown at 37 °C and were later subjected to Isopropyl β-d-1-thiogalactopyranoside (IPTG, Sigma-Aldrich, USA) induction at an OD600 ∼ 0.6-0.8 with 0.7 mM at 37 °C for 6 hours for PfPHB1-pGEX-4T 1 and 0.7 mM at 16 °C for 20 hours for PfPHB2-pGEX-4T 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in LB media and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (Sigma Aldrich, I1284). Bacteria pellets were resuspended in Bugbuster (Sigma Aldrich ...