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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for 6 Isopropyl 2 methylbenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Nuclei were identified using 4′,6-diamidino-2-phenylindole (DAPI, 1□μg/ml, Sigma Aldrich, Dorset UK). SDH staining was performed as previously described (Smith et al. ...
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bioRxiv - Immunology 2019Quote: ... then washed with 1% BSA/PBS and stained with 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Cell nuclei were stained with DAPI (1:1000, 4’ s-6-diamidino-2-phenylindole, Sigma Aldrich, USA) for 10 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and digested in 6 ml of 2-mg/mL type II collagenase (Sigma # C6885 or Worthington #LS004177) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclei were stained using 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Sigma Aldrich-Aldrich, Catalog No-D9542).
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with DAPI (4’,6-diamidino-2-phenylindole) (0.1 ng/μL, Sigma-Aldrich, cat. # D9542) for 5 min to exclude dead cells ...
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bioRxiv - Developmental Biology 2022Quote: ... 1x in wash buffer containing 0.1 μg/ml 4′,6-Diamidino-2-phenylindole dihydrochloride (Millipore Sigma, D8417), and 1x in wash buffer for 5 min each ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with the nuclear dye 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Germany) and actin filaments were labeled with ATTO 647-phalloidin conjugate (Hypermol EK ...
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bioRxiv - Neuroscience 2022Quote: ... and ≥99% 4-methyl-3-heptanol and 99% 6-methyl-5-hepten-2-one from Sigma-Aldrich (Item numbers M48309 and M48805-100ML ...
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bioRxiv - Neuroscience 2023Quote: ... at 1/1000 dilution or Laurdan (#40227, 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine, Merck, Sigma-Aldrich) at 1/500 dilution for 30 min at 37°C in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×105 KP cells (n=3) were plated in 6-well plates overnight in RPMI medium (Sigma). After 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... For nuclear staining 1 μg/ml of DAPI (4′,6′-diamidino-2-phenylindole) (Sigma Aldrich, Taufkirchen, Germany) was used ...
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bioRxiv - Cell Biology 2023Quote: ... stained for 10 min with 4′,6-diamidino-2-phenylindole (DAPI; Sigma, D9542, 1:1000 in PBS), and washed extensively in PBS before mounting in RapiClear® 1.52 (SUNJin Lab ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell nuclei were visualized using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, 1 µg/ml).
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bioRxiv - Cancer Biology 2023Quote: ... Cell nuclei were visualized using 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, 1 µg/ml). Fluorescence imaging was performed on a Nikon Eclipse Ti-E epifluorescence microscope ...
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bioRxiv - Evolutionary Biology 2022Quote: ... homogenates were stained with the fluorescent DNA marker 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Sigma-Aldrich), its volume was determined using an Eppendorf Xplorer 5–1000 μL electronic pipette (Eppendorf ...
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bioRxiv - Cell Biology 2023Quote: ... the DNA was stained with 5 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, D9542-5MG, SIGMA) diluted in 2% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... and (+−)-6-hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid (Trolox) (cat: 238813-5 G) were ordered from Sigma. Sodium hydroxide (cat ...
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bioRxiv - Cancer Biology 2023Quote: 2×105 KP cells (n=3) were plated in 6-well plates overnight in RPMI medium (Sigma). After 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×105 KP cells (n=3) were plated in 6-well plates overnight in RPMI medium (Sigma). After 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... along with DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride, Millipore-Sigma # 10236276001, used at 1 μg/mL), washed twice with PBSX for 15 minutes each ...
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bioRxiv - Cell Biology 2023Quote: ... and finally resuspended in FACS buffer containing 0.1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI, Sigma). Data collection occurred on a BD Biosciences LSRFortessa 3 and a gating strategy was used to isolate live ...
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bioRxiv - Bioengineering 2023Quote: ... Nuclear staining was performed through incubation of 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, D8417) diluted 1:250 in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... along with DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride, Millipore-Sigma # 10236276001, used at 1 μg/mL), washed twice with PBSX for 15 minutes each ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were stained with 4’,6-diamidino-2-phenylindole (DAPI; 0.5 μg/ml, Sigma-Aldrich, cat# D8417) diluted in PB to label cell nuclei ...
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VPS13B is localized at the cis-trans Golgi complex interface and is a functional partner of FAM177A1bioRxiv - Cell Biology 2023Quote: ... and (+−)-6-hydroxy-2,5,7,8-tetra-methylchromane-2-carboxylic acid (Trolox) (cat: 238813-5 G) were ordered from Sigma. 1× Phosphate Buffered Saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed thrice in PBS and incubated with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma, #D9542) diluted 1:10,000 in PBS for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... 6-diamidino-2-fenylindol (DAPI; 1:10 000; Sigma-Aldrich, Saint-Louis, MO, USA, 28718-90-3) and phosphate buffer (PB ...
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bioRxiv - Neuroscience 2024Quote: ... 6-diamidino-2-fenylindol (DAPI; 1:10 000; Sigma-Aldrich, Saint-Louis, MO, USA, 28718-90-3) and phosphate buffer (PB ...
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bioRxiv - Neuroscience 2024Quote: ... vibratome sections were counterstained with 1:1000 DAPI (4ʹ,6-diamidino-2-phenylindole, 1 mg/ml, Sigma) in PBS and incubated for 20 minutes in the dark ...
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bioRxiv - Physiology 2024Quote: ... hypothalamic tissue was homogenized in 100% methanol containing 1 mM 6-propyl-2-thiouracil (PTU) (Sigma, H34203) in a glass-glass tissue grind pestle (60mm ...
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bioRxiv - Cell Biology 2019Quote: ... coli BL21 cells and the expression of the transgene induced with 1 mM IPTG (isopropyl-β-D-thiogalatopyranoside, Sigma-Aldrich). The recombinant protein was purified with agarose beads that bind specifically to the His-tag (Ni-NTA Agarose ...
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bioRxiv - Cell Biology 2020Quote: ... the codon optimized gene encoding for rPMDs was subcloned into PET28a (+) and protein expression in E.coli cells was induced with 1 mM isopropyl-β-D-thiogalactoside (IPTG) (Sigma). The his-tagged proteins were purified using Ni-NTA chromatography and the concentration was determined using the BCA estimation kit (Pierce).
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bioRxiv - Biophysics 2022Quote: ... the proteins were expressed in Escherichia coli strain BL21 Star (DE3) cells using 0.4 mM Isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma) for 9 to12 hours at 20 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Induction of the expression systems was performed using varying concentrations of isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich, I6758).
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bioRxiv - Microbiology 2021Quote: ... and the expression was induced by the addition of 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich # I6758). After the addition of IPTG ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were expressed by induction at exponential growth phase (OD600nm≈0.5) with 0.5 mM isopropyl-β-D-thiogalactopyranoside (Sigma, I6758) for 2.5 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and CasX2 expression induced with the addition of 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, EMD Millipore, Burlington, MA). After 16 hours at 16°C ...
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bioRxiv - Cell Biology 2023Quote: ... Overexpression of the fusion protein was induced by adding 1 mM isopropyl-D-thiogalactopyranoside (IPTG; Sigma Aldrich; St. Louis, MO) to the transformed cell culture ...
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bioRxiv - Cell Biology 2023Quote: ... coli strains BL21 (D3E)/ pRARE carrying the corresponding expression vectors were induced for protein expression with 0.5 mM isopropyl-1-thio-D-galactopyranoside (IPTG, Sigma-Aldrich) for three hours at 30 ° C ...
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bioRxiv - Cell Biology 2023Quote: ... and overexpression of the proteins was induced by 0.1–0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma Aldrich, #I5502) for 20 hrs at 18°C for His6-tagged caltubin or 3 hrs at 30°C for GST-tagged caltubin ...
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bioRxiv - Plant Biology 2023Quote: Bacterial cultures were grown at 30 °C to an OD600 of around 0.8 in LB broth and induced with Isopropyl-β-D-thiogalactoside (IPTG, Sigma) for 15–18 h at 16 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the protein overexpression was induced by adding 0.5 mM (Cyt-b5) or 0.4 mM (FBD) isopropyl ß-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich). Cyt-b5 was overexpressed at 37 °C for ∼18 h and FBD was overexpressed at 30 °C for ∼16 hours ...
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bioRxiv - Biophysics 2024Quote: ... Gene expression was induced at OD600 ∼0.8 by addition of either 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG; Carl Roth GmbH) or 0.2 µM anhydrotetracycline (Sigma). Cells were grown overnight at 22°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Expression was controlled by T7 promoter and induced by 1 mM Isopropyl-β-D-thiogalactopyranosid (IPTG) (Sigma Aldrich, Steinheim, Germany) at an OD600 of 0.8-1.0 ...
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bioRxiv - Immunology 2019Quote: ... the stock solution of 6-hydroxydopamine (6-OHDA) (Sigma) was prepared in water at 42 mg/mL and stored at −20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with interleukin-6 (IL-6, Sigma) for 24 ...
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bioRxiv - Neuroscience 2023Quote: ... 6-OHDA (10mg/ml; 6-OHDA hydrobromide, Sigma-Aldrich) was dissolved in a vehicle solution immediately before the surgeries ...
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bioRxiv - Molecular Biology 2021Quote: ... and Glucose-6-phosphate dehydrogenase (G-6-PDH) with anti-G-6-PDH antibody (Sigma A9521). Blots were probed with IgG Goat anti-Rabbit (BioRad 170-6515 ...