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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for 1 Ethylbenz cd indol 2 1H ylidene malononitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... cholera toxin B subunit (CtxB)-FITC conjugate (C1655), methyl-β-cyclodextrin (Mβ-CD, C4555) and chlorpromazine hydrochloride (CPZ, C8138) were procured from Sigma.
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bioRxiv - Genetics 2021Quote: ... was generated by cloning the 3HA epitope CDS fused in-frame with the 3′ end of the RNAi resistant dCNBP CDS into the UAS-attB vector (Genewiz, SIGMA-ALDRICH). The plasmids for inducible expression of the human CNBP counterpart was generated by cloning the FLAG epitope CDS fused in-frame with the 3′ end of the hCNBP CDS (CNBP-201 splice variant ...
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bioRxiv - Immunology 2021Quote: ... Dams used as controls had free access to normal water and Cd-treated dams had free access to 10 ppm of CdCl2 (Sigma-Aldrich) dissolved in water ...
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bioRxiv - Developmental Biology 2024Quote: ... we switched to CD differentiation medium that comprised the same basic differentiation medium supplement with 10 nM arginine vasopressin (Sigma-Aldrich) and 10 nM aldosterone (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl of each stock was then spotted onto the surface of YPD plate (Yeast Extract 1%, Peptone 2%, Dextrose 2%, Agar 2%) without and with 1µg/mL tunicamycin (Sigma-Aldrich Inc., MI, USA). Plates were then incubated for 48-72 hours at 30°C before capturing their photographs ...
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bioRxiv - Immunology 2024Quote: ... T lymphocytes were adjusted at 50.000 cells/ml in their own culture medium and 0.5 ml of the cell suspension were transferred to 1.5 ml microcentrifuge tubes and incubated at 37°C for 2 hours in the absence or presence of metabolic inhibitors: 50 to 1 mM 2-deoxyglucose (2-DG) (Sigma, catalog: D8375-5G), 200 µM etomoxir (Sigma ...
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bioRxiv - Genetics 2021Quote: ... followed by a 1h 15min incubation at 45 °C with 10 μl of Proteinase K (10mg/ml Millipore, cat#70663). One volume of phenol/chloroform was then added to each lysate ...
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bioRxiv - Neuroscience 2021Quote: ... maintained for at least 1h at 4°C and then transferred onto semiporous membranes inserts (47 mm diameter, 0.45 µm pore size; Millipore FHLP04700) which were placed in six well tissue culture plates containing 1.1 ml medium per well ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with primary antibodies in BB for 1h at RT: monoclonal mouse anti-Col4a2 (Millipore, Cat. No. MAB1910), polyclonal rabbit anti-LM (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... resuspended in PBS for 15 minutes and then incubated 1h at 37°C with 60 µg/ml of PI (Sigma). Cells were collected by centrifuge and resuspended in ice-cold PBS for FACS analysis.
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bioRxiv - Biochemistry 2020Quote: The formation of homoserine lactone in the enzymatic reaction was confirmed by comparison with the 1H NMR spectrum of an authentic sample (from Sigma) dissolved in the same buffer ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed multiple times in TBS-T for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; Sigma) as above at 1:2500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Nitrocellulose membrane was washed in TBST for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; catalogue number A3562) (Sigma,) in 5% skim milk TBST at 1:2500 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... Germany) slides were incubated for 1h at room temperature (RT) in TBS containing 10% donkey serum (Sigma-Aldrich, Vienna, Austria), 1% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... were blocked for 1h in TBS containing 0.1% Tween-20 (TBS-T) supplemented by 3% fatty-acid free BSA (Sigma Aldrich) before incubation with the purified STARD10 protein (1 μg/mL in TBS-T + 3% BSA ...
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bioRxiv - Pathology 2021Quote: ... Fixed cells were then washed with 1X PBS and stained for 1h at room temperature with 0.1% crystal violet (Sigma-Aldrich) prepared in 10% ethanol/water ...
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bioRxiv - Immunology 2020Quote: ... for 1h at RT or addition of AP-conjugated goat anti-mouse IgM or IgA (Abcam and Sigma-Aldrich, respectively) and addition of pNPP substrate (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... Molten paraffin was then changed five times (each change lasting >1h) before the tissue was transferred into a Peel-A-Way embedding mould (Sigma) for transverse sectioning ...
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bioRxiv - Cell Biology 2024Quote: ... Bound material was eluted (1h at RT, shaking at 850 rpm) from anti-FLAG M2 magnetic beads (Sigma, Cat. # M8823) with homemade 10 µg/µL Strep-6xFLAG-peptide in PBS supplemented with 0.1% glyco-diosgenin ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs were extracted from untreated (0) and UV-C treated WT and jmj27 plants (time points 30 min, 1h and 2h) using Tri-Reagent (Sigma). Reverse transcription reaction (RT ...
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bioRxiv - Molecular Biology 2024Quote: ... DIvA cells were pretreated for 1h and during subsequent DSB induction with either 20µM ATMi (KU-55933 from Sigma, SML1109), 2µM ATRi (ETP-46464 from Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Permeabilisation was achieved by incubation for 1h in freshly prepared blocking solution (PBS with 5% (v/v) normal donkey serum (D9663, Sigma) and 0.3% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... incubated for 1h at RT in blocking buffer (BB: 10% Bovine Serum Albumin 0.3% Triton™X-100 (Sigma-Aldrich) in 1X PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Blocking was performed at room temperature for 1h using a PBS-based blocking buffer composed of 0.1% gelatin from cold water fish skin (Sigma-Aldrich), 1% BSA ...
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bioRxiv - Plant Biology 2024Quote: ... three washings in TBS-T were conducted and 1h incubation with the secondary antibody - Goat anti-Rabbit IgG (HRP) (Merk Millipore) diluted in TBS-T (1:5000 ...
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bioRxiv - Immunology 2024Quote: ... Sections were then blocked for 1h with 300 μl of 5% (v/v) donkey serum (Sigma-Aldrich, St Louis, MO) diluted in TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed three times for 5 min in PBS before blocking for 1h at room temperature with 3 % BSA in PBS-Triton 0.01 % (Triton X-100, Sigma, T8787). Cells were then incubated with primary antibody in blocking buffer (3 % BSA in PBS-Triton 0.01 % ...
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bioRxiv - Cancer Biology 2024Quote: ... then dissociated by mechanical (scalpel chopping) and enzymatic digestion (Collagenase A - from Clostridium histolyticum, Sigma-Aldrich, 1h at 37°C), within 2h of surgical resection or autopsy ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 1h at 37°C with secondary antibodies conjugated with oligonucleotides (Duolink probe PLUS and MINUS, Sigma). Coverslips were washed twice in Wash buffer A ...
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bioRxiv - Biophysics 2024Quote: ... and kept at 4°C for 1h in KB solution containing 50 mg/ml polyvinylpyrroli-done (PVP, Sigma, Chemical Co.). Finally ...
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Immune organization in sentinel lymph nodes of melanoma patients is prognostic of distant metastasesbioRxiv - Cancer Biology 2024Quote: ... Sections were then blocked for 1h with 300 µl of 5% (v/v) donkey serum (Sigma-Aldrich, St Louis, MO) diluted in TBS-T ...
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bioRxiv - Biophysics 2020Quote: ... 2 mM CaCl2 and 10 mM HEPES at pH 7.4 with 1-2 mM qBBr (Sigma-Aldrich or Toronto Research Chemicals ...
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bioRxiv - Microbiology 2022Quote: ... and 2 µg/ml L-1-Tosylamide-2-phenylethyl-chloromethyl-ketone (TPCK)-treated trypsin (Sigma-Aldrich, USA). Viral infectivity titers were measured in MDCK-II cells in 96-well plates ...
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bioRxiv - Neuroscience 2020Quote: ... protease inhibitor cocktail tablets and 2 mg/mL pepstatin A dissolved in ethanol:dimethyl sulfoxide 2:1 (Sigma). To minimize inter-blot variability ...
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bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
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bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were resuspended in 200µl of LCMS-grade acetonitrile/methanol/water (2:2:1) (Sigma Aldrich) chilled to -20°C and transferred to lysing matrix Y tubes (MP Biomedicals ...
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bioRxiv - Cell Biology 2024Quote: ... 1 ml ion exchange cartridge packed with 100 mg of 2– 2(pyridyl)ethyl silica gel (Sigma) was activated with 1 ml of methanol and equilibrated with 1 ml of buffer A (methanol/H2O 1:1 ...
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bioRxiv - Microbiology 2024Quote: ... at different concentration (1:1, 1:2, 1:10), 0.05% Tween-20 (BP337-100, Fisher BioReagents™) or 1% BSA (A6003, Sigma-Aldrich), was added on top of the concentrated spore suspension ...
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bioRxiv - Cell Biology 2020Quote: ... anti-alpha-tubulin (Sigma, Cat no. T5618, Clone B-5-1-2; 1:200), anti-Muc1 (Cell Signaling Technologies ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... mouse ascites MAb B-5-1-2 α-tubulin 1:200 (Sigma-Aldrich #T5168); rat TgEB1 1:100 (Chen and Gubbels ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Clone B-5-1-2) (1:1000, Sigma, Cat# T5168), and mouse anti-PyCSP (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-α-tubulin 1:10,000 (monoclonal B-5-1-2, Sigma-Aldrich). To image the blot the secondary antibodies goat anti-rabbit IgG IRDye 800CW (LI-COR ...
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bioRxiv - Developmental Biology 2022Quote: ... and diphospho-ERK-1 and ERK-2 (1:4000; clone MAPK-YT; Sigma; RRID:AB_477245). In our hands ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were picked up in a 1:1 mixture of 2% methylcellulose (Sigma-Aldrich) and 2.3 M sucrose ...
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bioRxiv - Plant Biology 2021Quote: ... 2 g L-1 Phytagel and 1.5 g L-1 activated charcoal (Sigma-Aldrich). After inoculation ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mM MgCl2) with 1% SDS and 1:2,000 Benzonase® nuclease (Sigma E1014). For tissue samples ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was mixed 1:1 with RIPA buffer with 2% sarkosyl (Sigma L9150) and incubated for 1 hour at room temperature with shaking ...
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bioRxiv - Microbiology 2022Quote: ... and then 2 ml overlay comprising 1:1 viral growth medium: 2.4% cellulose (Sigma) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), MCM2 (Abcam ...