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Citations for Millipore Sigma :
7401 - 7450 of 10000+ citations for Recombinant Human CYB5A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 30ug of total protein was subjected to a click reaction with 300μM Triazol (Sigma, ref. 678937), 50 μM biotin-alkyne tag (Thermo ...
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bioRxiv - Molecular Biology 2019Quote: Purified FLAG-tagged CSB proteins were immobilized on pre-equilibrated Anti-FLAG M2 Magnetic Beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was filtered through a 0.45 μm low protein-binding membrane (Steriflip HV/PVDF, Millipore). To concentrate lentivirus ...
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bioRxiv - Biochemistry 2021Quote: ... and protein concentrations of the supernatants were determined by the bicinchoninic acid assay (BCA1, Sigma-Aldrich). Lysates (30 μg per well ...
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bioRxiv - Cell Biology 2021Quote: ... eosinophil peroxidase (EPX) and Fibroblast specific protein-1 (FSP-1) were procured from Sigma-Aldrich (Merk). All other important reagents were procured from sigma other than specified Sigma-Aldrich (Merk).
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bioRxiv - Microbiology 2021Quote: RNA-binding protein immunoprecipitation (RIP) was performed using a Magna RIP kit (Cat# 17-700, Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The capture sensor surface was prepared by covalently immobilizing Protein A (Sigma-Aldrich, St. Louis, MO) to the chip surface using (1¬Ethyl-3-[3-dimethylaminopropyl]carbodiimide-hydrochloride)/N-hydroxysuccinimide (EDC/NHS ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were separated by SDS-PAGE and blotted onto Immobilon-P PVDF membrane (0.45 μm, Millipore) using a Trans-Blot Semi-Dry transfer Cell (BioRad) ...
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bioRxiv - Cell Biology 2019Quote: ... The purified fragment was separated by SDS-PAGE and protein was excised after InstantBlueTM (Sigma-Aldrich) staining ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein 5-HT2C or HCA2 (3 μg) was immobilized on nickel agarose beads (Sigma) in the incubation buffer containing 50 mM HEPES ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against glial fibrillary acidic protein (GFAP; 1:400; cat. no. Mab360; Millipore, Burlington, MA) and IBA-1 (1:500 ...
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bioRxiv - Biophysics 2019Quote: ... Elution of the proteins was done by supplementing buffer A with 400 mM Imidazole (Sigma-Aldrich). The labelled proteins were further purified by size-exclusion chromatography (Superdex 200 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was separated using 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon FL, Millipore). After blocking the membrane was incubated with primary antibodies-anti-TRF2 antibody (Novus Biological) ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Molecular Biology 2020Quote: Liver nuclear and cytosolic proteins separated by SDS-PAGE were transferred to polyvinylidene difluoride membrane (Millipore). The blots were blocked in a blocking solution (1%[w/v] skim milk in TBS [50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: ... The immunoprecipitation was performed by incubating the protein lysate with 1ug of GluA1 antibody (Millipore, ab1504) or 1ug HA antibody (Santa Cruz ...
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bioRxiv - Genetics 2021Quote: ... Proteins were separated using SDS-PAGE and transferred to 0.45-μm pore size PVDF membranes (Millipore). Membranes were blocked in 5% BSA for 1 h before overnight incubation with primary antibodies diluted in Tris-buffered saline with 0.1% tween (TBST) ...
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bioRxiv - Genetics 2020Quote: ... Epitope-tagged proteins were immunoprecipitated with either anti-Flag- or anti-HA-coated magnetic beads (Sigma and Pierce ...
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bioRxiv - Immunology 2021Quote: ... or porcine trypsin (PAR2 agonist, Type IX-S, 13000-20000 BAEE units/mg protein, Sigma Aldrich) or HBSS in a volume of 50 μL for 15 minutes at 37 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentrations of the resuspended protein pellets were determined by Bradford assay (Sigma-Aldrich, Taufkirchen, Germany) using bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein were purified with Glutathione Sepharose 4B (GE17-0756-01, Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... The collected protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut off and frozen at −80 °C.
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bioRxiv - Microbiology 2021Quote: ... Protein samples for mass spectrometry were purified and concentrated using ZipTipC-18 (Merck, Millipore, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2021Quote: ... The total cell lysates were precleared via incubation with protein G-linked agarose beads (Sigma; #1124323300) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... the culture medium was supplemented with 1 μM Rho-associated protein kinase (ROCK) inhibitor (Y27632, Millipore). Recombination was induced with 100 nM 4-hydroxytamoxifen (4OHT ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membrane (Millipore, Burlington, MA, USA). The membrane was blocked with 5% nonfat milk (BD bioscience ...
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bioRxiv - Biochemistry 2021Quote: ... Total proteins in the gel or on the membrane were visualized using Coomassie (InstantBlue, Sigma-Aldrich, scanned with the Odyssey or BioRad Chemidoc imagers ...
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... Puromycin incorporation into neosynthesized proteins was assessd with Western blotting and an anti-puromycin antibody (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... The dialyzed protein mixture was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) and the complex was further purified by size-exclusion chromatography (Superdex 200 increase 10/300 ...
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bioRxiv - Biochemistry 2021Quote: ... The dialyzed protein mixture was concentrated using a centrifugal filter (Amicon Ultra, MWCO 10 kDa, Sigma) and the complex was further purified by size-exclusion chromatography (Superdex 200 16/600 ...
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bioRxiv - Molecular Biology 2021Quote: Half of the native protein extract was pre-treated with 0.625 U/μL Benzonase (Millipore, E1014) at 37°C for 30 minutes ...
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bioRxiv - Zoology 2021Quote: ... The proteins were subjected to 8% polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Millipore). The membranes were then blocked in 5% (wt ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... The protein samples were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilon P, Millipore). The antibodies used are as follows ...
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bioRxiv - Neuroscience 2021Quote: ... irradiated 18% protein chow (Teklad Global) supplemented with 0.2% w/w bis(cyclohexanone) oxaldihydrazone (“cuprizone,” Sigma). Control mice received only the milled chow ...
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Biochemistry 2021Quote: ... Purified protein was concentrated using a 15-mL Amicon 10 kDa MWCO concentrator (Sigma Aldrich # UFC901024). Enzyme purity and identity were evaluated by SDS-PAGE and Western Blot (Anti-His).
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bioRxiv - Cancer Biology 2021Quote: ... 10 or 12% protein gels using SDS-PAGE and transferred onto PVDF membranes (EMD MIllipore-IPVH00010). Membranes were blocked in 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... 30 μg of proteins were mixed with 0.004% Bromophenol blue and 1% β-Mercaptoethanol (Sigma-Aldrich), incubated at 95°C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred to PVDF membrane by semi dry transfer apparatus in 45 minutes (Millipore). The blots were blocked for 1 hour in 5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants containing lentivirus were filtered through a low-protein binding syringe filter (0.45 μm, Millipore #SLHP033RS) before transductions ...
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bioRxiv - Biochemistry 2021Quote: ... The purified protein was concentrated using Amicon Ultra centrifugal filter devices (Millipore, cut off 10 kDa). The protein samples for dynamic light scattering (DLS ...
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bioRxiv - Cell Biology 2021Quote: ... The immune complexes were collected by incubation (2 h, 4°C) with protein G-agarose (Sigma). Co-IP assays were performed by using Pierce™ Protein G Agarose (Thermo Fisher ...
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bioRxiv - Genomics 2021Quote: ... Protein was transferred at 350 mA for one hour to Immobilon PVDF membrane (Millipore-Sigma # IPVH00010). Blots were blocked for one hour in PBST (1x PBS with 0.1% Tween ...
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bioRxiv - Genomics 2021Quote: ... Protein was transferred at 350 mA for one hour to Immobilon PVDF membrane (Millipore-Sigma # IPVH00010). Blots were blocked for one hour in PBST (1x PBS with 0.1% Tween ...
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bioRxiv - Immunology 2020Quote: ... Protein production was done in HEK293-6E cells by transient transfection with polyethylenimine (PEI, Sigma-Aldrich) and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were separated by SDS-PAGE on a 12% gel and transferred onto PVDF membrane (Millipore). Primary antibodies were ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to a PVDF membrane and incubated with mouse α- FLAG M2 monoclonal (Sigma) primary antibody ...
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bioRxiv - Microbiology 2020Quote: ... the protein was further concentrated using a 10 kDa Amicon Ultra-15 centrifugal unit (Millipore Sigma) and separated over a HiPrep 26/60 Sephacryl S100-HR sizing column (GE Healthcare Life Sciences) ...
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A Novel Mice Model of Catecholaminergic Polymorphic Ventricular Tachycardia Generated by CRISPR/Cas9bioRxiv - Molecular Biology 2021Quote: ... Proteins were resolved by SDS-PAGE and transferred onto polyvinylidene fluoride membrane (Millipore, Bedford, MA, USA). The membranes were incubated with a primary antibody against TECRL (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated on 12% SDS-PAGE gels and transferred onto 0.22 μm PVDF membranes (Millipore). Western Blots were performed in PBST with 0.5% milk using antibodies against the Myc tag (1:1000 ...