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Citations for Millipore Sigma :
7251 - 7300 of 10000+ citations for Recombinant Human CYB5A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... and the eluted protein was concentrated using the Amicon® Ultra 15 mL 3k device (Millipore) after being dialyzed for 24 h at 4°C against phosphate buffer saline (PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA sonication and immunoprecipitation of the protein/DNA complexes with specific antibodies (anti-Flag M2, Sigma) or without antibodies as a negative control ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were separated by SDS-PAGE and analyzed by immunoblotting using α-GFP (Sigma Aldrich, 11814460001) or α-FLAG (Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... Subsequent labeling of oxidative protein carbonyls was performed using an OxyIHC kit (EMD-Millipore, Cat#: S7450). Derivatization of protein carbonyl groups and all subsequent steps were performed in accordance with the manufacturers instructions ...
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bioRxiv - Biophysics 2020Quote: ... The diluted and refolded protein was concentrated to 20 ml using ultrafiltration Amicon stirred cell (Millipore) attached with a 10 kDa MWCO membrane (Stirred cell and Centriprep-10) ...
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bioRxiv - Immunology 2019Quote: ... We prepared protein lysates by scraping the cells in cold lysis buffer containing 1% Igepal (Sigma) and CompleteTM protease inhibitor cocktail and preclearing by centrifugation at 14,000 g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... proteins were separated and then transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-FL, EMD Millipore). Western blotting was performed using anti-HA primary (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: Protein extracts were separated on SDS-PAGE gel and were transferred onto polyvinylidene difluoride membrane (Millipore) using transfer buffer containing27mM Tris-Cl,48mM glycine,20% methanol for 1h in a Trans-Blot semidry electroblotter (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... the cells expressing FLAG fusion protein were harvested and lysed with CelLyticTM M lysis buffer (Sigma) containing protease inhibitor cocktail (Sigma ...
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bioRxiv - Biophysics 2019Quote: ... Protein was transferred overnight at 10 V to an Immobilon-P membrane (Merck Millipore; Burlington, MA) in 25 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... a 96-well plate pre-coated with a variety of ECM proteins was used (EMD Millipore; Collagen I ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies used were: rabbit anti-prosurfactant protein C (AB3786, EMD Millipore, Burlington, MA; 1:1000), rabbit anti-keratin 14 (KRT14 ...
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bioRxiv - Physiology 2020Quote: ... Protein was collected from the renal cortex and liver using CelLytic MT (Sigma-Aldrich, C-3228) plus protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant was collected and protein concentration was determined using the Direct Detect® Spectrometer (Merck Millipore). The proteins (30 µg ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for protein detection: anti-Flag M2 mouse 1/1,000 (F3165, Sigma), anti-myc 9E10 mouse 1/1,000 (sc-40 ...
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bioRxiv - Systems Biology 2020Quote: ... Protein samples were separated by SDS-PAGE and then transferred onto polyvinylidene difluoride membranes (Millipore, USA), before being blocked with 6% Rapid Block Buff II (Sangon Biotech ...
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bioRxiv - Bioengineering 2019Quote: ... proteins were separated by SDS-PAGE and transferred onto PVDF (polyvinylidene fluoride) membranes (Merck Millipore, DE). The membranes were then blocked for 1 h in 5% non-fat milk in PBST and incubated with CD64 and CD16 antibodies and β-actin antibody respectively overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... In order to reverse of TetR-CBX7 fusion protein binding 1 µg/ml Doxycycline (Sigma, D9891) was added to cell culture medium.
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Genetics 2020Quote: ... and diluted to measure protein concentration (1:10 diluted samples, Bradford Assay Kit, Sigma-Aldrich, B6916) and ATP level (1:2,000 diluted samples ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE and transferred to low fluorescence PVDF membranes (Immobilon-FL, Millipore). Membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
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bioRxiv - Neuroscience 2021Quote: ... Bottles were filled with either protein-containing solution (4% casein, sodium salt from bovine milk, Sigma #C8654 ...
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bioRxiv - Microbiology 2021Quote: ... proteins were then concentrated with a molecular filter (30 kDa cut-off, (Millipore China, Shanghai, China).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by 10% (wt/vol) SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore) that were then blocked in 5% (wt/vol ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins in the destained gel sections were reduced by incubation with 10 mM dithiothreitol (Sigma-Aldrich) at 56 °C for 1 h ...
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bioRxiv - Bioengineering 2021Quote: ... Four different target Fc-proteins were used in this study: hIgG (Sigma-Aldrich, St. Louis, MO), plant-expressed rCMG2-Fc ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoprecipitated proteins were eluted overnight at 4 °C with 50 μg of Flag peptide (Sigma-Aldrich) in 100 μL complemented non-denaturing lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The protein was quantified according to the Coomassie blue method using bovine serum albumin (Sigma-Aldrich) as a standard (Bradford ...
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bioRxiv - Neuroscience 2021Quote: We follow our previous publication [19] to perform total protein extraction using RIPA buffer (Sigma-Aldrich), SDS-polyacrylamide gel electrophoresis and western blotting ...
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bioRxiv - Biophysics 2021Quote: ... GST-GFP-RGG protein aliquots were concentrated with a centrifugal concentrator (Amicon Ultra Centrifugal Filters, Millipore). Proteins were then mixed with 20 mM Tris ...
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bioRxiv - Cancer Biology 2020Quote: ... The pre-cleared protein solution was incubated with 3 μg anti-FLAG antibody (Sigma Aldrich, #F1804) or IgG (Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... Membrane-immobilized protein detection used ECL Western Blotting Detection Reagents (GE Healthcare RPN2106; Millipore Sigma GERPN2106).
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bioRxiv - Cancer Biology 2020Quote: ... Protein was eluted twice from resin using 100μL of 500ng/μL [3x flag peptide (Sigma F4799), Myc (Pierce 20170) ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant containing proteins was collected after centrifugation and incubated in benzonase (EMD Millipore/Novagen, Country) at 37 °C for 30 min to shear chromatin ...
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bioRxiv - Cancer Biology 2019Quote: ... The supernatant containing proteins was collected after centrifugation and incubated in benzonase (EMD Millipore/Novagen, Country) at 37 °C for 30 min to shear chromatin ...
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bioRxiv - Biochemistry 2021Quote: ... the concentrated urinary proteomes were quantified via a 96 well plate Bradford protein assay (Sigma-Aldrich) using and Bovine Serum Albumin to generate the calibration curve.
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bioRxiv - Biochemistry 2021Quote: ... was initially subjected to affinity chromatography using a protein-A/anti-BSA mAB matrix (Sigma-Aldrich) to remove excess albumin (a heme-binding protein contaminant) ...
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bioRxiv - Neuroscience 2021Quote: ... and mouse monoclonal anti-glial fibrillary acidic protein antibody (G3893, Sigma-Aldrich, St Louis, MO, USA). Immunoreactivity to the antigen was visualized using Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Molecular Probes ...
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bioRxiv - Cell Biology 2021Quote: ... The calibration curve was established under the same conditions using the following standard proteins (Sigma MWGF1000): carbonic anhydrase (CAN ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... USA) or the fluorescent signal of each protein was measured with a chemiluminescence detection system (Millipore) using Scion Image software (Scion Corp. ...
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bioRxiv - Plant Biology 2020Quote: ... Bound effectors and associated plant proteins were competitively eluted by 3XFLAG peptides (Sigma-Aldrich, Dorsett, England) and separated by SDS-PAGE ...
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bioRxiv - Plant Biology 2020Quote: ... the mixed liposome and protein samples were analysed in Lumox®24-well plate (Millipore, USA) by live-cell imaging on Axio Examiner.Z1 (Zeiss microscope).
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bioRxiv - Systems Biology 2021Quote: ... the proteins the samples were reduced with 5 mM Tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich) for 20 minutes in 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were captured in ELISA plates pre-coated with 5 µg/mL of Avidin (Sigma) in Na2CO3/NaHCO3 buffer pH 9.6 ...
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bioRxiv - Microbiology 2020Quote: ... CCL2 and CCL5 protein levels were quantified using a sandwich ELISA procedure (Sigma, Cat# RAB0055 & RAB0077) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mg of total protein was then incubated with anti-Flag antibody (M2 monoclonal clone, Sigma) for 1 h with gentle rotation at 4°C and then protein-G coated magnetic beads (Thermo Fisher ...