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Citations for Millipore Sigma :
7301 - 7350 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... protein bands on the blots were developed with chromogenic substrate SigmaFast BCIP/NBT (Sigma). All blots were run at least twice to check for reproducibility ...
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred to a 0.2 μm polyvinylidene fluoride membrane (PVDF, Millipore, Billerica, USA) using a Bio-Rad semi-dry instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were semi-dry blotted onto a 0.2 µM nitrocellulose membrane (Sigma cat GE10600001) and protein levels were detected by specific antibodies directed against ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were separated by SDS-PAGE and were transferred to PVDF membranes (Millipore). All membranes were incubated with the indicated primary antibodies diluted in TBS-T (20mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2024Quote: ... iFluor 647-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake, Millipore Sigma SAE178-100UG); iFluor 488-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake ...
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bioRxiv - Bioengineering 2024Quote: ... iFluor 488-conjugated sAvPhire monovalent streptavidin (catalytic soluble protein uptake, Millipore Sigma SAE176-100UG); IRDye 680RD Donkey anti-goat (Western blotting ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were eluted with 50 µg/mL of 1x FLAG peptide (Sigma-Aldrich #F3290), concentrated using 30 kDa centricons (Millipore #UFC9030) ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using Amicon Ultra-0.5 10K Centrifugal Filter Devices (Merck Millipore). The protein concentration was measured on a Nanodrop 1000 spectrophotometer at 280 nm using the extinction coefficient of Trp and Tyr residues in the GST-LC3B sequence (48820 cm-1M-1).
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Biochemistry 2024Quote: ... Elution step of enriched proteins was performed using 2.25 M ammonia solution (Sigma, #5.33003) for 16 h ...
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bioRxiv - Biochemistry 2024Quote: ... The antibody-lysate complex was incubated with protein A- conjugated sepharose beads (Sigma #P3391) and eluted in 2X laemelli dye ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was filtered through a 0.45μm ultra-low protein binding filter (Merck Millipore). Aliquots were stored at −80 °C.
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 minutes at 60°C with 10 mM dithiothreitol (Sigma) in 25 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... the separated proteins were transferred onto a methanol activated 0.45 μM PVDF membrane (Millipore) at 70V for 120 minutes at 4 °C in Transfer Buffer (192 mM Glycine ...
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bioRxiv - Molecular Biology 2023Quote: ... The remaining protein was run through a 50 kDA cut off spin concentrator (Millipore) and dialyzed into 2X storage buffer (48 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... The M-protein solutions were diluted 1:1 with 8 M Urea (Sigma Aldrich) in Tris.HCl (pH=7 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted in two column volumes of 0.2 mg/ml 3xFLAG peptide (Sigma) and dialyzed against 50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were concentrated to >10 mg/mL using Amicon Ultra-15 Centrifugal Filters (Millipore). For some of the mutants assayed in this study ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with trypsin (1:50 µg/µg) (Sigma-Aldrich) and lysyl endopeptidase (1:75 µg/µg ...
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bioRxiv - Microbiology 2023Quote: ... Protein molecular weights were estimated from elution volumes using BSA (66 kDa) (Sigma-Aldrich) and ovalbumin (45 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... and glial fibrillary acidic protein (mouse anti-GFAP, 1:400, Sigma-Aldrich, cat. #: G3893), and cell nuclei were counterstained using Hoechst (10 µg/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were eluted in lysis buffer supplemented with 5% fresh β-mercaptoethanol (Sigma, M3148) and 1X SDS-PAGE loading buffer (5X SDS-PAGE loading buffer ...
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bioRxiv - Immunology 2022Quote: ... Proteins were reduced with the addition of 3 μL of 250 mM dithiothreitol (Sigma) at 37°C for 45 min and alkylated with 4.5 μL of 500 mM chloroacetamide for 30 min at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: ... The boiled proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The membranes were blocked in TBST (Tris-buffered saline ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM TCEP and mixed with 1:5000 SYPRO Orange Protein Gel Stain (Sigma). 50 μL of assay mixture was dispensed per well in a white bottom qPCR plate and each condition was performed in technical triplicate ...
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bioRxiv - Pathology 2023Quote: ... and proteins were electrophoretically transferred onto methanol-activated polyvinylidene fluoride (PVDF) microporous membranes (Millipore). Membranes were blocked for one hour with 5% bovine serum albumin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... the proteins were transferred onto PVDF membrane (Merck Millipore, cat. no. IPVH00010, Jerusalem, Israel). Prior to antibody incubation ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE and protein transfer to polyvinylidene difluoride (PVDF) membrane (Immobilon-P, Merck Millipore) was performed with the NuPAGE Novex and XCell II Blot Module systems (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with antibodies against OL markers proteins including Olig2 (MABN50, 1:500 Millipore, CA ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were concentrated by ultrafiltration on 10K Amicon® Ultra Centrifugal Filters (Merck Millipore), aliquoted ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: Total proteins were extracted from organoid cell pellets using 1X RIPA buffer (Sigma Aldrich) supplemented with 1X Halt™ protease and phosphatase inhibitor cocktail (Pierce) ...
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bioRxiv - Microbiology 2023Quote: ... Sized proteins were concentrated to >50 mg/ml using 10 kDa MWCO concentrators (Millipore), flash frozen on liquid nitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore-Sigma) centrifuged at 4,000 x g to no more than 3 ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore-Sigma) centrifuged at 4,000 x g to no more than 3 ml ...
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bioRxiv - Immunology 2023Quote: ... protein bands on the blots were developed with chromogenic substrate SigmaFast BCIP/NBT (Sigma).
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bioRxiv - Biophysics 2023Quote: ... Resolved proteins were then electrophoretically transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, USA) at 90 V for 2.5 h ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was discarded and the protein pellet was washed using guanidine hydrochloride (Millipore, #1.04220.0100 ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtrated using a 96-well filter plate (Millipore, Burlington, MA, USA) to remove precipitated protein and the A280 was remeasured before starting the binding assay ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein was then transferred to 0.4 μm polyvinylidene fluoride (PVDF, Merck Millipore) membrane ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore, Massachusetts, USA) and the membranes were blocked with PBS containing 5% non-fat dry milk for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by 10% SDS-PAGE gel and transferred onto PVDF membranes (Millipore). After blocked in 5% skim milk for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: Total protein from cortical organoids and HLCs were isolated using RIPA buffer (Sigma-Aldrich) consisting of protease and phosphatase inhibitors (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were collected from cells using ice cold RIPA buffer (R2078, Sigma Aldrich) with HaltTM 100x protease inhibitor cocktail added (78440 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were normalized with Milliplex® MAP Assay Buffer (EMD Millipore, Billerica, MA) to 6 μg protein per 12.5 μL for cytokine analysis and 1 μg protein per 12.5 μL for the MAPK pathway analysis ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted using 10 mM glutathione or 0.5 mg/mL 3XFLAG peptide (Sigma) in Lysis Buffer with 2 mM DDM.
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bioRxiv - Plant Biology 2023Quote: ... Expression of fusion proteins was verified by immunoblotting using either mouse anti-LexA (Sigma) or rat anti-hemagglutinin (HA ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K MWCO concentrator (EMD Millipore) to 500 µl and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing cleaved proteins was concentrated using a 30kDa MWCO concentrator (EMD Millipore) to 1mL ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated and transferred to Immobilon-P PVDF membrane (0.45 μm, Millipore Sigma). The membrane was blocked with 5% bovine serum albumin for 1 h and incubated overnight at 4°C with various antibodies (1:500 dilution for the PIKfyve antibody and 1:1000 dilution for all other antibodies) ...