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Citations for Millipore Sigma :
7101 - 7150 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The proteins were then transferred onto a PVDF transfer membrane (Immobilon, Millipore, Bedford, MA). The presence of transferred proteins on the membrane was confirmed by staining with Ponceau S (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... The flow-through containing the target protein was concentrated (Amicon 50 kDa cutoff, Millipore) and loaded onto a size-exclusion column (SRT-3C SEC-300 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins collected from size-exclusion chromatography were concentrated (Concentrators, Amicon Ultra, Millipore, Darmstadt, Germany), aliquoted and directly used for crystallization or stored at −80◦C.
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bioRxiv - Cell Biology 2022Quote: ... and immunoreactive proteins were visualised using Immobilon® Forte Western HRP Substrate (Merck Millipore). Densitometric analysis was carried out using the ImageJ 1.53c software ...
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bioRxiv - Biophysics 2022Quote: ... Protein expressions were induced by 1 mM isopropyl β-D-thiogalactopyranoside (IPTG, Sigma-Aldrich) overnight at 25°C ...
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bioRxiv - Biophysics 2022Quote: ... and NpSxph I559Y) Protein samples were filtered through a 0.22 µm spin filter (Millipore) before loading into the sample cell and titrated with STX (300 µM STX for RcSxph D794 and 100 µM STX for RcSxph ...
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bioRxiv - Cell Biology 2022Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K MWCO concentrator (EMD Millipore) to 500 µl and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated proteins were transferred on to 0.45 µm PVDF membrane (Merk Millipore, #IPVH00010), followed by blocking with 5 % skim milk in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration for lysates was determined using BCA reagent (Sigma-Aldrich, St. Louis, MO), and equal amounts of protein (50 μg/lane ...
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bioRxiv - Cell Biology 2022Quote: RIP assay was done with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). Quantitative RT-PCR was used for determining total RNA levels.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinyl difluoride (PVDF) membranes (Millipore) according to standard protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... [1] using the protein A sepharose beads (GE Cat # 17-0780-01; Millipore-Sigma). The concentrated BCG-CM (containing 1.0 mg protein in the lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was eluted with sonication buffer containing 50 mM reduced glutathione (Sigma-Aldrich) and dialyzed against storage buffer [50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were developed using Luminata Classico or Luminata Forte HRP substrate (Millipore Sigma) and detected using an Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Tiam1 protein was purified using the ANTI-FLAG® M2 affinity gel (Sigma-Aldrich) followed by Superdex 75 ...
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bioRxiv - Cell Biology 2022Quote: Proteins were separated in polyacrylamide gel and then transferred to polyvinylidene difluoride membranes (Millipore). These membranes were incubated with primary antibodies ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein extracts were incubated with 5 μl T7 antibody (EMD Millipore; Cat # 69522) for 4 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were initially cross-linked to DNA with 1% formaldehyde (Sigma, Cat. No F8775) for 15 minutes in 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the clear protein lysate was deposited in a Microcon-30kDa filter unit (Millipore, MRCF0R030) and centrifuged at 14,000g for 20 min to pass the totality of the sample through the filter ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by a 30 min incubation with 30 µl protein-A Sepharose (Sigma- Aldrich). Protein complexes were washed three times by centrifugation (2.700×g ...
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bioRxiv - Microbiology 2020Quote: ... Test proteins were re-suspended as purified or dialysed with U-tube concentrators (Novagen) into 50 mM Tris-HCl pH 7.5 ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... and then pre-washed with 50 μl of protein G agarose bead slurry (Millipore) at 4° C for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were stained using primary antibodies against β-actin (1:10,000, AC-15, Sigma), strep II-tag (1:1,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... the lysate was mixed with 100 µl of protein A-sepharose beads (Sigma-Aldrich), pre-washed five times with 1 ml of 20 mM Tris-HCl ...
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bioRxiv - Systems Biology 2020Quote: ... Fusion proteins were then expressed in the Rosetta P3 DE LysS E.coli strain (Novagen) using an autoinduction protocol (Jolma et al ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to H3K27me2/3 ChIP samples and Protein A agarose (40 µL; Sigma) was added to GFP ChIP samples and incubated for 3 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS–PAGE and transferred to PVDF membranes (Immobilon-P; Millipore) in a submerged tank ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated with the Quantipro BCA assay kit (Sigma-Alrich, United States), and lysates were resolved on a 10% sodium dodecyl sulfate polyacrylamide gel prior to transfer onto polyvinylidene fluoride membranes ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then concentrated using a 10 kDa cutoff centrifugal filter unit (Millipore) and chromatographed on a Superdex 200 gel filtration column in a final buffer of 20 mM HEPES pH 7.0 ...
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bioRxiv - Neuroscience 2021Quote: ... dilute proteins were concentrated using Amicon Ultra 15mL centrifugal filters (Merck Millipore, 10kDa NMWCO) and subjected to single-step buffer exchange into SEC running buffer (see SAXS method ...
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bioRxiv - Biochemistry 2021Quote: ... and the protein was concentrated using Amicon 3 kDa MWCO centrifugal filters (Merck Millipore). The protein concentration was calculated using the measured absorbance at 280 nm under native conditions and assuming an extinction coefficient of 19940 M-1 cm-1.
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bioRxiv - Immunology 2020Quote: ... 40 uL of Salmon Sperm DNA/Protein A Agarose-50% (Millipore, Cat #16-157C) for 30 min spinning at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Supernatant was first incubated with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h to remove background signal ...
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bioRxiv - Neuroscience 2020Quote: ... 30-40µg of the total protein was combined with 2X sample buffer (Sigma-Aldrich) and boiled for 10 mins before loading in 11% SDS-PAGE ...
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bioRxiv - Biophysics 2020Quote: ... pH 7) and filtration of the labeled protein through 0.22 µm filter (Millex, Millipore).
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bioRxiv - Neuroscience 2022Quote: ... Protein was diluted in Laemmli sample buffer with 5% 2-Mercaptoethanol (Sigma-Aldrich M6250) and denatured for 5 mins at 95°C ...
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bioRxiv - Neuroscience 2022Quote: ... The protein samples were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were blocked with 3% BSA + 0.05% Tween-20 for 1 h before incubation with primary antibodies overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Immunoreactive proteins were visualized using an enhanced chemiluminescence detection system (Millipore, Bedford, MA, USA).
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bioRxiv - Immunology 2022Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were transferred from the gels to Immobilon PVDF membranes (Merck Millipore Ltd.). The membranes were immersed in appropriate blocking buffer (Tris-buffered saline 0.05% Tween 20 (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... Purified proteins were concentrated using Amicon Ultra® 10,000 MWCO centrifugal filter units (Millipore) or Microsep™ Advance 3kDa (PALL ...
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bioRxiv - Genetics 2022Quote: ... Protein bands were developed with Immobilon Western Chemiluminescent HRP Substrate solutions (Millipore Sigma #WBKLS0050) and visualized under UV light using BioRad’s ChemiDoc MP™ Imaging Platform ...
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bioRxiv - Developmental Biology 2022Quote: ... The proteins were transferred from polyacrylamide gel onto a polyvinylidene difluoride membrane (Millipore IPVH00010). After the transfer ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21(DE3) as a fusion protein containing an N- terminal His6-tag (Novagen) and purified by metal-affinity chromatography (Ni-NTA ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were eluted from pooled beads with 0.5mg/ml FLAG peptide (#F3290, Millipore Sigma) by shaking at 1000 rpm for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... and immunoprecipitated proteins were eluted 3 times with 150µL 3x-FLAG peptides (Sigma, F4799) for 25 min each at 25°C ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Immobilon-FL, EMD Millipore, Billerica, MA) and probed using antibodies against tau (Tau-5 ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies used included anti-rabbit glial fibrillary acidic protein (GFAP, Sigma, 1:400), mouse anti-Zrf1 (Gfap ...