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Citations for Millipore Sigma :
7201 - 7250 of 10000+ citations for 6 CHLORO 4' METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... the sperm pellet was washed in IVF–TALP medium and a final concentration of 1 × 106 spermatozoa/mL was adjusted using IVF–TALP medium enriched with BSA (Sigma A8806; 6 mg/mL) and heparin (25 mg/mL).
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bioRxiv - Microbiology 2022Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 supplemented with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO) at 37°C followed by stimulation with peptide pools for 12 h ...
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bioRxiv - Microbiology 2022Quote: ... 6-Carboxyfluorescein filled liposomes were separated from the unincorporated 6-Carboxyfluorescein by size exclusion chromatography using Sephadex G 25–300 (Sigma Aldrich, Saint Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... CreERT2 expression was activated in young adult mice (6-8 week-old) by administering tamoxifen (Sigma, T5648-1G, 20 mg/ml in corn oil) by subcutaneous injection (100mg/kg ...
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bioRxiv - Neuroscience 2024Quote: ... One group of mice (referred to as DMI + 6-OHDA) was pre-treated with one injection of desipramine hydrochloride (DMI, Sigma-Aldrich, 25mg/kg i.p.) 30 minutes before the 6-OHDA infusion in order to protect the noradrenergic system [50].
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bioRxiv - Cell Biology 2023Quote: Non-cornmeal molasses food was freshly made with or without 100 µM or 5 mM Phloroglucinol (PHG) (Sigma-Aldrich, CAS number 108-73-6) and solidified in a vial overnight ...
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bioRxiv - Immunology 2024Quote: ... and culture supernatants were harvested after 24 h for analysis of cytokines (CXCL1 and IL-6) and MMP2 activity (Luminex bead array, Millipore Sigma, St. Louis, MO).
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bioRxiv - Immunology 2023Quote: ... 300,000 WT T84 cells per well in a 6-well plate were treated with 20 µL lentivirus using 3 µL Polybrene transfection reagent (Sigma Aldrich #TR-1003-G) diluted in 3 mL media ...
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bioRxiv - Neuroscience 2023Quote: Rescue of cholesterol accumulation were performed by adding the drugs to cultures previously incubated overnight with Bodipy-cholesterol and further incubated for 6 h at the following concentrations: Evodiamine (Sigma, stock 1000x in DMSO) 10 µM ...
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bioRxiv - Physiology 2023Quote: Hepatocytes were isolated from C57BL/6 and SIRT4 KO mice (8–12 weeks old) by perfusing the liver tissue with collagenase type IV (Sigma-Aldrich, catalog no. C5138) containing media as described in Shaw et al ...
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bioRxiv - Microbiology 2023Quote: ... The extracts were diluted from 1:10-3 to 1:10-6 in 10 mM MgCl2 for plating so that spreading of 50 μl extract with a delta cell spreader (Sigma-Aldrich, St. Louis, USA) resulted in a density of 100-300 CFU on square plates (12 x 12 cm ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then centrifuged at 4,000 rpm for 22 min at 4°C and the supernatants were transferred to another set of 6 eppendorf tubes containing a mixture of pre-washed beads (Millipore Sigma, GE17-0886-01) and 12.5 uL (12.5 ug ...
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bioRxiv - Microbiology 2023Quote: ... the media was replaced and supplemented with 200 ng/mL aTc and either DMSO or 6 μg/mL brefeldin A (Sigma-Aldrich catalog number B6542). After an additional 16 h ...
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bioRxiv - Microbiology 2023Quote: ... Cells (5 × 104 cells/cm2) were grown in triplicate in 24-well plates pre-coated with 6 μg fibronectin (Sigma-Aldrich catalog number FC010) and transfected the next day with 500 ng pRL0381 or pRL0385 with TransIT-LT1 ...
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bioRxiv - Immunology 2023Quote: ... bone marrow cells were obtained from femurs and tibiae of WT C57BL/6 mice by flushing with complete RPMI 1640 media containing 10% heat inactivated fetal bovine serum (FBS) (Sigma-Aldrich, non-USA origin). Red blood cells were lysed using ACK lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... neurospheres were collected or attached to standard culture plates in media containing 5% FBS for 6 h and stained with crystal violet solution (Sigma-Aldrich, 548-62-9).
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bioRxiv - Cell Biology 2024Quote: ... or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Neuroscience 2021Quote: ... Cells fixed in 4% PFA were blocked in PBS containing 5% donkey serum (Sigma-Aldrich #D9663) and 0.3% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... after which cells were fixed with 4% paraformaldehyde (Fisher) and stained with Napthol Blue Black (Sigma). One image per well was collected using an Olympus OM-D E-M5 Mark II digital camera ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with following primary antibodies at 4 °C overnight: TRA-1-60 (Merck Millipore), OCT3/4 (H-134 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were rinsed with phosphate buffered saline (PBS) thrice and fixed with 4% paraformaldehyde (Sigma Aldrich) in PBS for 15min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cells were rinsed with phosphate buffered saline (PBS) thrice and fixed with 4% paraformaldehyde (Sigma Aldrich) in PBS for 15min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Bacteria were harvested by centrifugation and resuspended in BugBuster® Protein Extraction Reagent (Millipore #70584-4) supplemented with protease inhibitor cocktail (cOmplete EDTA-free ...
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bioRxiv - Cell Biology 2020Quote: ... Resulting supernatants were incubated with anti-FLAG M2 magnetic beads (Sigma, 3 h at 4°C). Beads were washed thrice with TUBE lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Wako Lab Chemicals) for 4 hours at RT before dilution with ammonium bicarbonate (20 mM; Sigma) and digestion with trypsin (1/50 (w/w) ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in the same buffer (4 ml each) containing HRV 3C protease (32 units each; Novagen), and incubated without agitation (4°C ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM EGTA overnight at 4°C and immunolabeled with phosphorylated histone H3 (serine10) antibody (Millipore). To remove fixation solution ...
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bioRxiv - Cell Biology 2020Quote: ... cultured hippocampal slices were cut on both sides of CA1 and 4 µM 2-chloroadenosine (Sigma) was present in all recording experiments ...
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bioRxiv - Cell Biology 2020Quote: ... before being filled with 4 l of 0.5x phosphate buffered saline (PBS) made from tablets (Sigma) dissolved in ultrapure water ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... cells were fixed with 4% paraformaldehyde for 20 min and stained with DAPI (D9542, Sigma-Aldrich) in 1xPBS for 10 min ...
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bioRxiv - Immunology 2021Quote: ... was loaded at 4 μM for 60 min in the presence of 0.04% Pluronic F127 (Sigma)and 1.25 mM probenecid (Cayman) ...
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bioRxiv - Developmental Biology 2021Quote: ... The larvae were flipped and transferred to a microcentrifuge tube containing 4% Paraformaldehyde (PFA, Sigma, P6148) in 1X PBS for 25 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted octamer fractions were pooled and concentrated using centrifugal filters (Amicon Ultra-4, 10k M.W.C.O., Millipore) to a final concentration of 5-15 µM ...
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bioRxiv - Developmental Biology 2020Quote: ... oocytes were fixed for 10 min at 4°C with pre-cooled 10% trichloroacetic acid (Sigma). Next ...
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bioRxiv - Developmental Biology 2020Quote: ... the mice were anaesthetized and injected (intraperitoneal) with BrdU [dissolved in 4 mg BrdU (Sigma-Aldrich) in 300 µl of saline/per mouse] ...
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bioRxiv - Microbiology 2021Quote: ... The blocks were infiltrated with a 4:1 mixture of propylene oxide (PO):Durcupan resin (Sigma) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: Mice were deeply anesthetized with pentobarbital (Eutasil) and perfused transcardially with 4% paraformaldehyde (P6148, Sigma-Aldrich). The brain was removed from the skull ...
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bioRxiv - Neuroscience 2021Quote: ... at 4°C followed by a contrasting step with 1% thiocarbohydrazide (Sigma-Aldrich, St. Louis, USA) for 1 h at room temperature and 1.5 h incubation with 2% OsO4 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibodies overnight at 4°C: sheep anti-TH (Millipore, #AB1542) at 1:1000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The left hemisphere was immediately fixed in 4% paraformaldehyde (PFA; Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany) for immunohistochemistry ...
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bioRxiv - Neuroscience 2021Quote: ... and were killed by transcardial perfusion / fixation with 4% paraformaldehyde (Cat number P6148 Sigma-Aldrich, UK) in 0.1 M phosphate buffer.
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bioRxiv - Bioengineering 2022Quote: ... and 4 μL of nuclease-free water in each well on the 1.2% agarose gel (Sigma) stained with Ethidium Bromide (1 mg/μL ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos were washed with PBS-0.2% Tween and incubated overnight at 4 °C with DAPI (Sigma) diluted 1:1000 in PBS-0.2% Tween ...
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bioRxiv - Genomics 2020Quote: ... The supernatant was stored at 4°C with 0.02% sodium azide and protease inhibitors (Sigma, P8849).
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bioRxiv - Molecular Biology 2019Quote: ... at 1μM concentration 4) Deferiprone (L1, Sigma Aldrich, Saint Louis, MI, USA, CAS: 30652-11-0) at 50μM concentration ...
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bioRxiv - Developmental Biology 2019Quote: ... the chicken embryos were first chemically fixed using 4% of formaldehyde buffered solution (Sigma, ref. 1004965000) for 2 or 16 hours at 4 °C and then washed twice with PBS ...
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bioRxiv - Developmental Biology 2020Quote: Hair cells in neuromasts were visualised using the vital dye 4-Di-2-ASP (Sigma-Aldrich) as previously described (Sapède et al. ...
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bioRxiv - Neuroscience 2019Quote: ... All cells used in this study were exposed to 1 µM 4-hydroxytamoxifen (Sigma, Cat#: H6278) for 24 hours prior to studies of Ucp2 function.
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bioRxiv - Cell Biology 2019Quote: ... Membranes were blocked in 4% milk for 20 minutes and were probed with anti-FLAG (Sigma) for 4 hours at room temperature or overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... 4°C for 5 min and washed once with a PBS containing 1% BSA (Sigma Aldrich). The single cell suspensions used for 10X Genomics scRNAseq analysis were cleared of red blood cells by using a Red blood cell lysis buffer (eBioscience ...