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Citations for Millipore Sigma :
7101 - 7150 of 10000+ citations for 6 CHLORO 4' METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The reaction was quenched by incubating with 4 mM phenylmethylsulfonyl fluoride (PMSF; 52332, Sigma-Aldrich) for 5 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... 4 dpf larvae were exposed to 2.5 μg/ml of Neutral Red (Millipore Sigma: N7005) in embryo water for 3 hours at 28.5°C ...
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bioRxiv - Developmental Biology 2024Quote: ... stage 3 to 20 HH chicken embryos were fixed in 4% paraformaldehyde (PFA)(158127, Sigma) diluted in PBS 1X at 4 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated for 45min at 4°C with FLAG-magnetic beads (Sigma-Aldrich #M8823). The unbound fraction was discarded ...
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bioRxiv - Cancer Biology 2024Quote: Agents used include All-Trans retinoic acid (RA) (CAS no. 302-79-4 Sigma-Aldrich) and Palbociclib (Cat no ...
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bioRxiv - Neuroscience 2024Quote: ... mice were deeply anesthetized and perfused with cold saline followed by 4% paraformaldehyde (Sigma-Aldrich) in 0.1 M phosphate buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were visualised using Luminata™ Forte western HRP substrate (Millipore, WBLUR0100, 4 min exposure). To normalise the signal ...
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bioRxiv - Pathology 2024Quote: ... tissues were stained overnight at 4°C with rat anti-laminin (Sigma L0663, 1:1000) in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... slices were mounted on microscope slides using Mowiol 4-88 (Sigma Aldrich, cat no 81381). For both studies ...
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bioRxiv - Neuroscience 2024Quote: ... a specific KIX-KID interaction inhibitor was used (CAS 92-78-4; Millipore/Sigma Aldrich) was added at a concertation of 2.5µM.
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bioRxiv - Neuroscience 2024Quote: ... a specific KIX-KID interaction inhibitor was used (CAS 92-78-4; Millipore/Sigma Aldrich) was added at a concertation of 2.5µM.
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was performed at 4°C overnight with 120μl α-FLAG M2 beads (Sigma-Aldrich) followed by two 10-minute washes using lysis buffer without EDTA ...
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bioRxiv - Neuroscience 2024Quote: ... 0,2% sodium taurodeoxycholate hydrate and 3 mM 4-methylumbelliferyl β-D-glucopyranosidase (Sigma-Aldrich, M3633) and incubated at 37°C for 90 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... cells on coverslips were washed twice with PBS then fixed using 4% paraformaldehyde (Sigma Aldrich) for 15 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: The cells on 12-mm circular glass coverslips were fixed with 4% paraformaldehyde (Sigma-Aldrich) and washed with PBS ...
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bioRxiv - Bioengineering 2024Quote: ... followed by quenching with hydroxylamine hydrochloride (631.7 mg, 9.09 mmol, 4 eq., #159417 Sigma-Aldrich) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: EV preparations and 4 × 106 BV2 cells were lysed in RIPA lysis buffer 1X (Sigma) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation with PI (4 mM) and Hoechst (0.5 mg/mL) (Sigma-Aldrich, USA) at 37°C for 10 min ...
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bioRxiv - Systems Biology 2024Quote: ... each with 4 thoraces were homogenized in 100 µl of 5 M guanidine-HCl (Sigma) supplemented with 100 mM Tris pH 8.0 and 5 mM EDTA pH 7.8 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... overnight at 4 °C followed by cryopreservation in 30% (w/v) sucrose overnight (Sigma Aldrich) at 4 °C ...
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bioRxiv - Biophysics 2024Quote: Thoraces from 2–3-day-old adult Drosophila were dissected in 4% paraformaldehyde (PF, Sigma), and indirect flight muscles were isolated as described previously (Katti et al ...
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bioRxiv - Systems Biology 2024Quote: ... mouse tissue sections were thawed and fixed with 4% v/v Formaldehyde (Sigma-Aldrich F8775) in 1x PBS for 30 min at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: PAs from homozygous a2KO (n = 5) and WT (n = 6) embryos were manually dissected in ice-cold DEPC-treated phosphate-buffered saline (DPBS, Sigma catalog number D8537). Individual PAs were pooled into four samples (WT PA1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Up to 180mL of blood was collected into 3 60 mL syringes pre-loaded with 6 mL of anticoagulant acid citrate dextrose (ACD) (Sigma C3821 Lot#SLBW6172). Within 15 min of collection ...
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bioRxiv - Cell Biology 2019Quote: Cold KIF3A purification buffer (50 mM NaPi, 400 mM KCl, 2 mM MgCl2 × 6 H2O, 0.001% Brij35, 0.75 mM β-mercaptoethanol, Sigma#M3148, 0.2 mM ATP) supplemented with protease inhibitors and DNaseI was added to frozen BL21-RIL cell pellets expressing a KIF3A construct ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated for 30 min with 10 µM 6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) and 1mg/L Hoechst 33342 dye (Sigma Aldrich, United States) diluted in media ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore, Billerica, Massachusetts, USA). The resulting 1 ml sample was loaded on top of a DG ...
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bioRxiv - Genomics 2020Quote: ... We followed the protocol as described previously [6] with one alteration: instead of using one paperdisc (Whatman qualitative filter paper, WHA1001070, SIGMA-ALDRICH CO LTD) per extraction ...
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bioRxiv - Neuroscience 2020Quote: Male CD-1 mice aged 6-10 weeks were given an ip injection of 3 mg/kg LPS from Salmonella typhimurium (Sigma, St. Louis, MO) dissolved in sterile normal saline at 0 ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2021Quote: ... per well of a 6 well plate of differentiated human neural cultures (~2 months old) along with 3μg/ml of polybrene (Sigma-Aldrich #TR-1003-G), pipetting the LV concentrate directly onto the culture (drop-wise) ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... primary cortical neurons were cultured for six days in 6-well-plates followed by treatment with the mTOR inhibitor temsirolimus (10 μM in DMSO; Sigma, St. Louis, USA) or Torin 1 (500 nM in DMSO ...
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bioRxiv - Plant Biology 2020Quote: ... and their petioles dipped in 1 mM 5(6)-Carboxyfluorescein diacetate (CFDA, Ex/Em 492/517 nm, Millipore-Sigma, St. Louis, MO, USA) for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... and their petioles dipped in 1 mM 5(6)-Carboxyfluorescein diacetate (CFDA, Ex/Em 492/517 nm, Millipore-Sigma, St. Louis, MO, USA) for 5 min ...
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bioRxiv - Plant Biology 2021Quote: ... medium, containing 3% sucrose, 10−6 M potassium indole acetate (Nacalai Tesque, Kyoto, Japan) and 10−5 M kinetin (Sigma, St. Louis, MO) at 25 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred to 6 well culture dishes and rehydrated with MilliQ water and labeled in H2O for 5 min with Dapi (Sigma-Aldrich, Cat # 9542) 1:10,000 dilution of 1 mg/ml aqueous stock and Syber Gold 1:20,000 dilution of DMSO stock (ThermoFisher Cat # S11494) ...
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bioRxiv - Bioengineering 2022Quote: ... Wild type C57BL/6 mice were sacrificed and were perfused ventricularly with sodium heparin solution in PBS (H3393-100KU, Sigma-Aldrich, MO, USA). The lungs were then excised and fixed in 4% PFA overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: 12- week-old C57BL/6 male mice were injected intraperitoneally with CCl4 (HPLC grade, Cat#270652, Sigma, diluted 1:3 in mineral oil) or mineral oil (control ...
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bioRxiv - Neuroscience 2021Quote: AIA was induced in male and female mice on a C57BL/6 background using the antigen methylated bovine serum albumin (mBSA, Sigma, St Louis, MO) as described previously26 ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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bioRxiv - Biophysics 2019Quote: ... Membrane was washed another 6 times in TBS-T then developed in the dark for 5 min with chemiluminescent reagent (Immobilon Forte, Millipore, Burlington, MA USA).
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bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore, Tullagreen, Carrigtwohill, IRL). The retentate was recovered and incubated with 100 µl of Micrococcus luteus peptidoglycan (1 mg/ml ...
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bioRxiv - Developmental Biology 2019Quote: Fasting plasma samples collected from offspring at PN30 and during the GTT at 6-7 months of age were analysed for insulin levels using a rat insulin radioimmunoassay kit (SRI-13K, Millipore Australia, Kilsyth, VIC). Insulin samples were run in duplicate at 1:5 ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were seeded in 6-well plates (3.0 × 105 cells/well) coated with poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 24 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... female mice received subcutaneous injections (s.c.) of corn oil (CO) (25 ml/kg, vehicle control; n = 6) (#C8267-2.5L, Sigma-Aldrich, St Louise, MO, USA) or a low-dose of TCDD (20 ng/kg/d ...
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bioRxiv - Bioengineering 2019Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG 2000, Laysan Lipids, Arab, AL) with 6:1:2:1 ratio in propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80 °C until all the lipids were dissolved ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were infected with the viruses in the presence of 6 μg/mL polybrene (Cat. #107689; Sigma-Aldrich, St. Louis, MO, USA). The overexpressing vectors were transfected into Ink4a/Arf-/- astrocytes using the NeonTM Transfection System 10μl Kit (Cat ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... boric acid and ascorbic acid as well as reagents required for 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) synthesis were obtained from Sigma-Aldrich (Bangalore, India). Acetone was obtained from Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...